| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,600,471 | G→A | 12.9% | A221T (GCC→ACC) | zntA → | zinc, cobalt and lead efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,600,471 | 0 | G | A | 12.9% | 69.3 / 3.5 | 33 | A221T (GCC→ACC) | zntA | zinc, cobalt and lead efflux system |
| Reads supporting (aligned to +/- strand): ref base G (15/13); new base A (2/2); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-01 | |||||||||||
TCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTGGGCG > CP009273/3600334‑3600613 | tCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCcgcgcg > 1:175014/1‑149 (MQ=255) aaaTCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCcgcgcgcg > 2:106220/1‑149 (MQ=255) aaTCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGt > 1:49740/1‑149 (MQ=255) aaTCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCGGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCcgcgcgcgg > 1:376665/1‑148 (MQ=255) ccGGCAGCTACTTCGCCATTGAAACCGTAATGACCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCcgcgcgctgcag < 2:56917/149‑6 (MQ=255) ttCGCCATTGAAACCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAgcgc < 1:306188/149‑1 (MQ=255) tCGCCATTGAAACCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGcgcg > 1:373263/1‑149 (MQ=255) tCGCCATTGAAACCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATAGGCGCAACTGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGcgcg < 1:130779/149‑1 (MQ=255) aTTGAAACCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAAt < 1:75085/149‑1 (MQ=255) aaaCCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGgcg > 1:199769/1‑149 (MQ=255) ccTTAATGAGCGTAGCCGCTATTGGGGCACGGTTTATTGGCGCAACGGCTGAAGCTGCGATGGGGGTGCTGCTGGTTTTGGTTGGTGAACCGCTGGAAGGCGCGGCCGCCAGCCGCGCGCGGCAGGGCGGTAGCGCGTTAATGGCGCTg > 2:242152/1‑149 (MQ=255) aaTGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAAc < 2:270457/149‑1 (MQ=255) gggCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAg < 2:246860/147‑1 (MQ=255) gCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGaa < 1:106220/149‑1 (MQ=255) cACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGt < 2:293173/149‑1 (MQ=255) cTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTaa < 1:321948/149‑1 (MQ=255) tttATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCACCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAggg > 2:18016/1‑149 (MQ=255) tttATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCACCAGCCGCGCGCGTCAGGGCGTTAGCGCGATAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAggg > 2:240440/1‑149 (MQ=255) cAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGaa > 1:127351/1‑149 (MQ=255) gATGGTGTTGCTGCTGTTTTTGGGTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGATACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACa < 2:300726/149‑1 (MQ=255) gtgtTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCt > 1:238773/1‑149 (MQ=255) ttttGAGTTGTCAACGCCCGGAAGGTCGGTCCCCCAGCCGCCCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCCGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGAt < 2:15978/149‑1 (MQ=255) ttGATTGGTGAACGACTGGAAGGCTGGGCCACCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGAtgt < 1:18016/149‑1 (MQ=255) ttGATTGGTGAACGACTGGAAGGCTGGGCCACCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGCGGCGCTTAACAGCCTGCGCCCTGGCGAtgt < 1:240440/149‑1 (MQ=255) tGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTg < 1:21693/149‑1 (MQ=255) gTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGaa > 1:288010/1‑149 (MQ=255) gTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGaa < 2:397114/149‑1 (MQ=255) cGACTGGAAGGCAGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCCCGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTcgc < 1:80223/149‑1 (MQ=255) aCTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGCTGTGATTGAAGTcgccg > 2:102934/1‑149 (MQ=255) tGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCa > 1:125246/1‑149 (MQ=255) gCTGGGCCGCCAGCCGCCCGCGTCAGGGCGTTAGCGCGTTGGCGCCGCTGAAACCCGAAAGCTACACAAACTTGCGTAAGGGTGACCGGGAAGAGGGGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTggg > 2:450514/1‑149 (MQ=255) cTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTCATTGAAGTCGCCGCAGGTGGGc > 1:72224/1‑149 (MQ=255) tGGGCCGCGAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGGGAGCGGGAAGAGGTGGCACTTAACGGCCTGCGACCTGTCGATGTGATTGAACTCCCCGCACGTGGGCg > 2:10993/1‑149 (MQ=255) | TCAAATCCGGCAGCTACTTCGCCATTGAAACCTTAATGAGCGTAGCCGCTATTGGTGCACTGTTTATTGGCGCAACGGCTGAAGCTGCGATGGTGTTGCTGCTGTTTTTGATTGGTGAACGACTGGAAGGCTGGGCCGCCAGCCGCGCGCGTCAGGGCGTTAGCGCGTTAATGGCGCTGAAACCAGAAACCGCCACGCGCCTGCGTAAGGGTGAGCGGGAAGAGGTGGCGATTAACAGCCTGCGCCCTGGCGATGTGATTGAAGTCGCCGCAGGTGGGCG > CP009273/3600334‑3600613 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |