| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 982,560 | T→A | 100% | intergenic (‑122/+481) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 982,560 | 0 | T | A | 93.7% | 40.2 / ‑3.1 | 16 | intergenic (‑122/+481) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (6/9); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Not supported by required number of reads on both strands. | |||||||||||
CAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATT > CP009273/982418‑982701 | cAGCATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGAAGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTc < 1:134057/149‑1 (MQ=255)cAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGAAACCTGCCCCTGCCCTCCATAAGTTCTGTAAATAAAAATTTACGGAAATATTGATAAGAGTCTGGTTTCTTTATGTTTCTGCAGACCTCTTACCATTc > 2:142915/1‑149 (MQ=255)cAGAATATCGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTc < 2:370941/149‑1 (MQ=255) tcctcatTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCg < 1:170558/146‑1 (MQ=255) tatttattACCCGCATGGTTTTTTTTATGACACCTGCCACTGCCGTCCATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGt < 1:294080/149‑1 (MQ=255) tccTGCCACTGCGGTCAATAAGTGCGGTTAATAAAAATTTAGGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGGCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTc < 1:38578/148‑1 (MQ=255) cTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAATTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaa > 2:304253/1‑149 (MQ=255) cAATAAGGTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAatat < 1:216753/149‑1 (MQ=255) tAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTaaaa < 1:115197/149‑1 (MQ=255) tAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTaaaa > 1:226709/1‑149 (MQ=255) aaaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCGGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAAttt > 1:76398/1‑149 (MQ=255) tacgaatgaGAGTTTTGGCTCCTTATCTTTTTCGAGCCATCTTACCATTCAAACTAACGACTAGCTACCGTAAAAGTGCCAAACACAAAAAAATATTTATACAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgtg < 2:284254/144‑1 (MQ=255) gagaGTTGGGTGTCTTTATGCGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTAAAAAAAAAAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCg < 2:454437/149‑1 (MQ=255) agTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCa > 1:46121/1‑149 (MQ=255) tgtCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTcc < 1:316925/149‑1 (MQ=255) gCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGCGACGTGATGATAtt > 2:443189/1‑149 (MQ=255) | CAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATT > CP009273/982418‑982701 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |