| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,506,454 | C→T | 28.5% | intergenic (‑269/‑67) | mntH ← / → nupC | manganese/divalent cation transporter/nucleoside (except guanosine) transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,506,454 | 0 | C | T | 28.5% | 50.0 / 19.4 | 35 | intergenic (‑269/‑67) | mntH/nupC | manganese/divalent cation transporter/nucleoside (except guanosine) transporter |
| Reads supporting (aligned to +/- strand): ref base C (9/16); new base T (3/7); total (12/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCACTGCTGGTAAGCAGCGACCGC > CP009273/2506320‑2506595 | gAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCa > 1:425871/1‑149 (MQ=255) ggttatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATa > 1:118402/1‑149 (MQ=255) gttatgTTTAATGTTAGCACCTTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACAtcg < 2:135464/149‑3 (MQ=255) tatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGaa < 2:25840/149‑1 (MQ=255) tatgTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGaa < 1:115983/149‑1 (MQ=255) tatgTTTAATGTTAGCACATCTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGaa < 2:365494/149‑1 (MQ=255) atgCTTAATGTAAGCACATTTACATAACTTTCAGCTTCCATACACAATCTAGCAGAAACGTATGACAGATCACTATTTTGGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAAt < 1:130255/149‑1 (MQ=255) tttAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAAtgtg > 1:444687/1‑149 (MQ=255) aGCACATTTACATCACTTTCAGCTTCCATACACAACACAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGCCCCATGCCGCCATTATACTCTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGaact < 2:157076/149‑3 (MQ=255) cacaTTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAAttt < 2:142079/149‑1 (MQ=255) acaTTTACATAACTTTCAGCTTCGATACACAACAAAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTa > 1:257524/1‑149 (MQ=255) tttACATAACTTTCAGCTTCCACACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCATATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAAcc < 2:125259/149‑1 (MQ=255) tAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCTTGTTCCCTCGAACGTCCACGGAACTTTAACCTGcccc > 2:229835/1‑147 (MQ=255) cAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTg < 1:422856/149‑1 (MQ=255) cAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTg < 2:286032/149‑1 (MQ=255) gCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGa < 2:260530/149‑1 (MQ=255) cATACAAAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTGGCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCaaa < 1:225072/149‑1 (MQ=255) tACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCGTTGTAGTGGGTGTCAGATATCGTTGTCCTTAACCATGTTGCATAGGATGTGCACGGAAATTTAACCTGCCTCATGTTGGGAGCAAata > 2:515/1‑149 (MQ=255) acaacaTAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTCGAGCAAATATgg > 1:244833/1‑149 (MQ=255) aacaTAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGAc < 1:324124/149‑1 (MQ=255) gAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCtt < 2:118402/149‑1 (MQ=255) gACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTa > 1:436739/1‑149 (MQ=255) cAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACt < 1:29357/149‑1 (MQ=255) aGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATTTTCACGTAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTg < 2:425871/149‑1 (MQ=255) aGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTGACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTg < 2:261869/149‑1 (MQ=255) tATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTg > 1:291615/1‑149 (MQ=255) gCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGc > 1:133465/1‑149 (MQ=255) atgACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGc < 1:393639/147‑1 (MQ=255) ggACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCa < 2:369319/149‑1 (MQ=255) gTCATCAGAGTGTGTGTCAGATCTTGTCTTCCTTAACCATTTTACATAGCATGTTCACGGAAATTTACCCCGCCTCATTTTTGGAGCAACTATGGACCGCGTTCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCCctgc < 2:178199/149‑1 (MQ=255) atCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCActgc < 2:380565/148‑1 (MQ=255) aTTATAGTGTGTGTCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGACTCATCTTTGGAGCAACTCTGGACCGCGTCCTTCATTTTGTAATGGCACTTGCCGTTGTTGCGATTCTCGAACTGCggg > 1:96682/1‑149 (MQ=255) gtgtgtgtCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCACTGCTGGTAagca < 2:18640/149‑1 (MQ=255) tgtgtCAGATCTTGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCACTGCTGGTAAGCAGCg < 2:436739/149‑1 (MQ=255) cAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTCCGATTCTCGCACTGCTGGTAAGCAGCGACCGc > 2:59458/1‑149 (MQ=255) | GAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCAGCTTCCATACACAACATAGCAGAAATGTATGACAGATCACTATTTTTGAAGCCTGTCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCTGCCTCATATTTGGAGCAAATATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCACTGCTGGTAAGCAGCGACCGC > CP009273/2506320‑2506595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |