Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,371,135 G→T intergenic (‑356/+39) sspA ← / ← rpsI stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,371,1350GT96.9% 169.1 / NA 65intergenic (‑356/+39)sspA/rpsIstringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base T (33/29);  minor base A (2/0);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCA  >  CP009273/3370991‑3371279
                                                                                                                                                |                                                                                                                                                
tGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGGCTGTCAGGCGGCAACAAAAAGCAACTTTCCAGTTTTTACGCCGAATCAGACTTTAGCTATAAAAACACCCGCCGAAGCGGAtttt                                                                                                                                              >  2:469826/1‑149 (MQ=255)
 gcgcTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGtttttt                                                                                                                                             >  1:418382/1‑149 (MQ=255)
          tCTTAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAAtt                                                                                                                                    <  2:895703/149‑1 (MQ=255)
          tCTGAACTAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCTAAAAGCAACTTTCCAGTTTTTACTCTTATTCAGATTTTAGCTATAAAAAAACCCGCCGAATCGGTTATTTTAGAAAAtt                                                                                                                                    >  1:509133/1‑149 (MQ=255)
              aaaTAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGttt                                                                                                                                >  1:1154551/1‑149 (MQ=255)
                        cTTAGTCCCTTATTCGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCAGATTCTGATTTTATCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAg                                                                                                                      <  1:750734/149‑1 (MQ=255)
                           aGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAg                                                                                                                   >  1:10904/1‑149 (MQ=255)
                               ccTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAAACCGCCGAAGCGGATTTTTTCAAAAATTGTTTTCTGCCGGAGCAGAAGc                                                                                                               >  1:435800/1‑149 (MQ=255)
                                  tATTGGCTATGTGGTTTTTGTTTTACCTGTCTGTCTGTTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCaa                                                                                                            <  1:554837/149‑1 (MQ=255)
                                          aTGTGTTTTTTGTTTTACCTGTCTGTCAGGTGGAAGCAAAAAGGAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGTTATAAAAAAACCCGCCGAAGCAGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGtt                                                                                                    <  1:464626/149‑1 (MQ=255)
                                          aTGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGtt                                                                                                    <  2:418382/149‑1 (MQ=255)
                                              ggTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGACAATTAACGTTTGga                                                                                                >  1:464750/1‑149 (MQ=255)
                                              ggTTTTTGTTTTACCTGTCGGTCAGGTGGCAGCAAACAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGga                                                                                                <  2:1073280/149‑1 (MQ=255)
                                               gTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgag                                                                                               >  1:994379/1‑149 (MQ=255)
                                               gTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACCCTGATTCAGATGTTAGCTATAAAAAAACACGCCAAAGCGATTTTATTCGAAATTTGTTTAGTACCGGGGCAGAAGACAATTAACGttgagag                                                                                               >  1:4581/1‑144 (MQ=255)
                                                 ttttGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGaa                                                                                             >  1:587373/1‑149 (MQ=255)
                                                   ttGTTTTTCCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACt                                                                                           <  1:294788/149‑1 (MQ=255)
                                                   ttGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACt                                                                                           >  1:398247/1‑149 (MQ=255)
                                                     tttttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACTCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGc                                                                                         <  1:1019529/148‑1 (MQ=255)
                                                     gTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAATTTTACTGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGTTGTGTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGc                                                                                         <  1:1024593/149‑1 (MQ=255)
                                                            ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTCTAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGGGAACGGCGaacgac                                                                                  >  2:69082/1‑149 (MQ=255)
                                                            ctgtctgtCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgac                                                                                  >  2:1079978/1‑149 (MQ=255)
                                                              gtctgttAGGTGGGAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacga                                                                                <  2:465280/149‑1 (MQ=255)
                                                              gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacga                                                                                >  1:1101145/1‑149 (MQ=255)
                                                              gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAAAGTTTGGAGAACTGCGaacggcga                                                                                >  2:838373/1‑149 (MQ=255)
                                                               tctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGATGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgacgac                                                                               <  1:506334/149‑1 (MQ=255)
                                                                 tgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTTGATTTTTTTTTTCTGCCGGAGCCACAGCCAATTAACGTTTGGAGAACTGCGgccgcggcggg                                                                             >  2:187576/1‑142 (MQ=255)
                                                                   tcAGGTGGCAGCAAAAAGCGACTTTCCAGTTTTTACGCTGATTCGGATTTTAGCTATAAAAAACCTCGCGGCGGCGGTTTTTTTCGAACATTGTTTTGTGCCGGAGCAGAAGCCAGTTTACGTTTGGTGACCTGTGGAGGAGGGCGTgg                                                                           >  2:161926/1‑148 (MQ=255)
                                                                   tcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTATGACGGAGCAGAAGCCAATTAACATTTGCAGAACTGCGGACAACGACGTGc                                                                           >  1:415850/1‑149 (MQ=255)
                                                                       gTGGCAGCAAAAAGCAACTTTCCAGATTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTCTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATAAACGTTAGGAGAACAGCGGACGACGACGTGCttaa                                                                       >  2:117571/1‑147 (MQ=255)
                                                                          gcagcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTAAGc                                                                    >  1:220861/1‑149 (MQ=255)
                                                                          gcagcaAAAAGCAACTTTCCAGTATTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGGGCTTTACGc                                                                    >  1:685258/1‑149 (MQ=255)
                                                                            agcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAg                                                                  >  1:1010283/1‑149 (MQ=255)
                                                                            agcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAg                                                                  >  1:809356/1‑149 (MQ=255)
                                                                             gcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGa                                                                 >  1:1107625/1‑149 (MQ=255)
                                                                              taaaaaGCAATTTTCCAGTTTTTATGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGAc                                                                <  1:787382/148‑1 (MQ=255)
                                                                              caAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGAc                                                                <  1:983833/149‑1 (MQ=255)
                                                                              caAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGAc                                                                <  1:1019572/149‑1 (MQ=255)
                                                                              caAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTAACGCAGAc                                                                >  1:714424/1‑149 (MQ=255)
                                                                               aaaaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGAcc                                                               >  2:215323/1‑149 (MQ=255)
                                                                                      aaCTTTCCAGGTGTTACGCTGATTCAGATATTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTTTTTTCTGCGGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTc                                                        <  2:1025407/149‑1 (MQ=255)
                                                                                             cAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGtt                                                 <  1:978810/149‑1 (MQ=255)
                                                                                             cAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCCGTCGGAGACGGGCTTTACGCAGACCGACTTTCTTACGtt                                                 <  1:907860/149‑1 (MQ=255)
                                                                                                tttgtACTCTGATTCATATTTTGACTATAAAAAACCCCGCCGAAGCTGTTTTTTTCGAAAATTTTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCaa                                              <  2:669603/149‑1 (MQ=255)
                                                                                                        cTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCTGAAGCAGAAACCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTGTCTTAAGTTCAACCTGACGa                                      >  1:541713/1‑149 (MQ=255)
                                                                                                         tGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACAACGATCTATACGCAGACCGACTTTCTTACGTTCAACCTGACGAg                                     >  1:1146831/1‑149 (MQ=255)
                                                                                                         tGATGCAGATTTTAGCTATAAAAAACCCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAg                                     <  2:509305/149‑1 (MQ=255)
                                                                                                          gagtCAGATTTTAGCTAAAAAAAAACCCGGCGAAGCGGTGTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGc                                    <  1:212929/146‑1 (MQ=255)
                                                                                                             tCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGGGTc                                 >  1:1074380/1‑149 (MQ=255)
                                                                                                             tCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTc                                 >  1:822791/1‑149 (MQ=255)
                                                                                                              cAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCa                                <  2:464750/149‑1 (MQ=255)
                                                                                                               aGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCAc                               <  2:10904/149‑1 (MQ=255)
                                                                                                               aGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCAc                               <  1:215323/149‑1 (MQ=255)
                                                                                                               aGATTTTAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGAAGACGAAAAATAACGTTTGGAGAAATGCGGACGACGACAGGCTTTACGCAGAGCGGCTTTGTTACGGTAAACCGGACGAGCGTcgc                               >  2:242384/1‑147 (MQ=255)
                                                                                                               aGATTTTAGCTAGAAAAGAACCCGCTTGAGCGTTTTTTTTCGAAAATTGTTTTCGGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCAc                               >  2:724028/1‑149 (MQ=255)
                                                                                                                  ttttAGCTATAAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAg                            <  2:1154551/149‑1 (MQ=255)
                                                                                                                         tataAAACAATACGCCGATGTGGTTTATTTCGAAAATTTTTTGCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGaa                     <  2:1157716/149‑1 (MQ=255)
                                                                                                                         tataAAAAAACCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGaa                     >  1:1139060/1‑149 (MQ=255)
                                                                                                                              aaaaaCCCGCCGAAGCGGTTTTTTTCGAAAATTGTTTTATGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGCGTAACGAAGCCAg                >  2:887428/1‑149 (MQ=255)
                                                                                                                                 aaaccgcttcggCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTCTCGCGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCGGACGAGCGTCACGAGTAACGAAGCCGGCtt             >  2:506028/12‑149 (MQ=255)
                                                                                                                                    ccgccgAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGAGTCACGAGTAAAGAAACCACCTTTAc          >  1:262306/1‑149 (MQ=255)
                                                                                                                                    ccgccgAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACAAGCGTCACGAGTAACGAAGCCAGCTTTAc          >  1:104546/1‑149 (MQ=255)
                                                                                                                                     cgccgAAGCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACg         <  2:587373/149‑1 (MQ=255)
                                                                                                                                      gccgAAGGGGTTGTTTTCGAAAATGGGTTTCTGGCGGAGCAGAAGCCAATTAACGTTGGGAGAACTGCGGACGACGATGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGc        <  1:30204/149‑1 (MQ=255)
                                                                                                                                         gAAGGGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACTTTTGGAGAACTGCGGACGACGACGTGCTTTACCCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAATCCAGCTTTACGCAGt     <  2:777819/149‑1 (MQ=255)
                                                                                                                                            gCGGTTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCTGCTTTACGCAGTTCa  <  2:685258/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCA  >  CP009273/3370991‑3371279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: