| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 3,676,832 | G→T | intergenic (‑25/+158) | dctA ← / ← yhjK | C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,676,832 | 0 | G | T | 98.2% | 169.5 / NA | 57 | intergenic (‑25/+158) | dctA/yhjK | C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (28/27); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGCCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATC > CP009273/3676686‑3676977 | aaGCCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGt < 1:383980/149‑1 (MQ=255) ccGTCGCCAAGCGGTTTCATTTGCTCGCCTATTACAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTCTTAAACAGAGAGGTTTTCATAGGGTGTCCTGTAGTAAAACCACATGTCtt > 1:324596/1‑149 (MQ=255) gCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAg < 2:264663/149‑1 (MQ=255) cAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGACAGCAAAGGCATTTGAGAAAACAGGCTTTCATGGCGTGTCCTTTAGTAAAACCACATGCCTTGTAAGGt > 1:442185/1‑149 (MQ=255) aGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCCTTAGTAAAACCACATATCTTGTAAGGtca > 2:37050/1‑147 (MQ=255) aGCGGTTTCATTTGCTCGCCCATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTa < 2:603873/149‑1 (MQ=255) cGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATg < 2:479764‑M1/149‑2 (MQ=255) cGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATg < 1:323740‑M1/149‑2 (MQ=255) cGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCCATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATg < 1:510422‑M1/149‑2 (MQ=255) gTTTCATTTGCTCGCCGATTTGAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggg < 1:754141‑M1/149‑4 (MQ=255) cATTTGCTCGCCTATTTCAGGATAGGAATGGGCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATTTCTTGTAAGGTTATggggtac < 2:953978‑M1/149‑8 (MQ=255) aTTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGGTTTCATAGGGTGTCCGTTAGTAAAACGACAGGTCTTGTAAGGTTATggggtacc > 2:985057‑M1/1‑141 (MQ=255) aTTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATAGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACACAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACTACATGTCTTGTAAGGTTATggggtacc > 2:985034‑M1/1‑141 (MQ=255) cTCGCCTATTTCAGTATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcgg < 1:769971‑M1/149‑14 (MQ=255) cTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcgg < 1:100987‑M1/149‑14 (MQ=255) tCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggg < 2:314372‑M1/149‑15 (MQ=255) cGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAGCCACATGTCTTGTAAGGTTATggggtacctgcgggg < 2:422534‑M1/149‑16 (MQ=255) tCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcg < 2:379832‑M1/149‑24 (MQ=255) cAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGGTATggggtacctgcgggggacctgggg > 1:157840‑M1/1‑125 (MQ=255) cggaTAGAGATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCAAATGTTTTAATTTTTTAGttggtacctgcggggtacctgcggc < 1:243983‑M1/148‑26 (MQ=255) aGGATAGAAAGGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggc < 1:130405‑M1/149‑26 (MQ=255) tAGAAATGGCCAAGGAGAATACCAATGGCTATCGGTGGCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGGTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggcctta > 2:92532‑M1/1‑120 (MQ=255) aaaTGGCCAAGGAGAATACCAATGGATATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGAGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaa < 1:616249‑M1/149‑33 (MQ=255) tGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataa < 1:454908‑M1/149‑36 (MQ=255) tGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataa > 1:402891‑M1/1‑114 (MQ=255) cAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCCTAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacc > 1:140203‑M1/1‑110 (MQ=255) aaGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggagaacatgcgagttaccttgggcctacccaacacactc > 1:229861‑M1/1‑109 (MQ=255) aaGGAGAATACCAATGGCTATCGCTGGCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtaccggcggccttaaaataacaccc > 1:173264‑M1/1‑109 (MQ=255) cAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatca < 1:414014‑M1/149‑52 (MQ=255) aTGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcaca < 1:713491‑M1/149‑54 (MQ=255) tATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGGGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaatgacagccagacaacatccaagaaat > 1:419638‑M1/1‑91 (MQ=255) tATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGATTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcgggggacctgcggccttaaaatgacaccccgacaacatcacaggaat > 1:576895‑M1/1‑91 (MQ=255) tCGCTGTAAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcccagaaatgt > 2:263691‑M1/1‑89 (MQ=255) aGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATgggatacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggat > 2:135687‑M1/1‑81 (MQ=255) gACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTCTggggtacctgcggggtaccctcgtccctaaaataacacccagacaacatcacagaaatgtacctggatca > 1:935356‑M1/1‑79 (MQ=255) ctggaggtaaaggCGGTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACTTGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataa > 2:739406‑M1/7‑76 (MQ=255) cTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataa > 2:436889‑M1/1‑76 (MQ=255) tGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTCGTAACACTACATGTCTTGTAAGGTTATgcggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataat < 1:741688‑M1/149‑75 (MQ=255) gAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgcacctggatcataatt > 2:994630‑M1/1‑74 (MQ=255) gTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaa < 1:869479‑M1/149‑80 (MQ=255) gTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaa < 1:823850‑M1/149‑80 (MQ=255) gCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaa > 1:419448‑M1/1‑64 (MQ=255) aaaCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagcaatgtacccggatcataattgaaacaacaaggttaa > 2:428409‑M1/1‑58 (MQ=255) aaaCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaacgttaa > 2:428322‑M1/1‑58 (MQ=255) aaCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcatacttgaaacaaaaaggttaaa > 2:626274‑M1/1‑57 (MQ=255) cAGAGAGGTTTTCATGGGGGGGCCTTTAGTAAAACCACATGTCTTGTAAGGTTATgggttacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataatttaaacaaaaaggttaaaaa < 2:271810‑M1/149‑95 (MQ=255) gagGTTTTCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagttt < 1:436889‑M1/149‑99 (MQ=255) tCATAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtcacaggtttatatttttaaccaaaacgttaaaaagtttgaactgg > 1:931006‑M1/1‑44 (MQ=255) aTAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagtttgagctggct > 1:660760‑M1/1‑42 (MQ=255) tAGGGTGTCCTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagtttgagctggctc < 1:37050‑M1/149‑109 (MQ=255) cTTTAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggctcataattgaaacaaaaaggttaaaaagtttgagctggctcggacaaacc > 1:514362‑M1/1‑32 (MQ=255) tttAGTAAAACGACATGTCTTGTAACGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagtttgagctggctcgcacaaacca < 2:543955‑M1/149‑119 (MQ=255) tttAGTAAAACCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagtttgagctggctcgcacaaacca > 2:209825‑M1/1‑31 (MQ=255) tAAAACCAAATGGCTTGTAGGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagtttgagctggctcgcacaaaccagcact < 2:140203‑M1/149‑124 (MQ=255) aaaCCACATGTCTTGTAAGGTTATggggtacctgcggggtacctgcggccttaaaataacacccagacaacatcacagaaatgtacctggatcataattgaaacaaaaaggttaaaaagtttgagctggctcgcacaaaacagcacttt > 1:176890‑M1/1‑24 (MQ=255) ccACACGTCTTTTAAGGTTATggggtacctgcggtgtacctccggccttaaaataacacccagacaacatcacataaaggtacctggatcataattgaaacaaaaaggttaaaaagtttgagctggctctcacaaaccagcacttttta < 2:886742‑M1/149‑129 (MQ=255) acaTGTCTTGTAAGGTTATggggtacgtgcggggtacctgcggccttaaaataacacccagacaacagcacagaaatgtaccgggatcataattgaaacaaaaaggttaaaaagtttgggctggctcgcacaaaccagccctttttaaa > 2:468635‑M1/1‑19 (MQ=255) | AAGCCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATC > CP009273/3676686‑3676977 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |