| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 927,772 | Δ1 bp | 12.5% | intergenic (‑266/‑279) | trxB ← / → lrp | thioredoxin reductase, FAD/NAD(P)‑binding/leucine‑responsive global transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 927,772 | 0 | G | . | 12.5% | 102.0 / 13.0 | 40 | intergenic (‑266/‑279) | trxB/lrp | thioredoxin reductase, FAD/NAD(P)‑binding/leucine‑responsive global transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (15/18); new base . (2/3); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 | |||||||||||
CCCGTCATTATCACCTCTGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGTCTTC > CP009273/927633‑927920 | cccGTCATTATCACCTCGGCTACTTAAATTTCCCGCTTTATATGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGC‑AAAACATTCg < 2:562806/149‑1 (MQ=255) gTCATTATCACCTCTGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAg > 2:525152/1‑149 (MQ=255) tATCACCTCTGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGa < 1:562322/143‑1 (MQ=255) tccccTCTGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaa < 2:210516/146‑1 (MQ=255) ccTCTGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaa < 2:296098/149‑1 (MQ=255) tctGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCCTGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGgaagaa > 1:490870/1‑143 (MQ=255) tctGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaa < 2:221874/149‑1 (MQ=255) cTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAAc > 1:464995/1‑143 (MQ=255) ttAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTGTTGCGAAAACATTCGAGGAAGAAAAAAAACa > 1:128265/1‑143 (MQ=255) ctataTTTCCCGCTTTATAAGTCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTGTTGCGAAAACATTCGAGGAAGAAAAAAAACa < 1:322132/139‑1 (MQ=255) ttCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTc > 1:419104/1‑143 (MQ=255) ccGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGC‑AAAACATTCGAGGAAGAAAAAAAACAGTATTCTtat > 1:485515/1‑143 (MQ=255) ccGCTTTATAAGCAGATTAAATGATGAAGAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGC‑AAAACATTCGAGGAAGAAAAAAAACAGTATTCTtat > 1:37784/1‑143 (MQ=255) cGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGACGAAACAAAACCGTATTCTtat > 1:330608/1‑143 (MQ=255) ataAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATgcgc < 1:248177/143‑1 (MQ=255) ccGATAAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACcatgca < 2:545375/149‑1 (MQ=255) gaATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACACTCGCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGAATTCTTAGATGCGCATAACCATGCATGTAAAt > 1:371175/1‑143 (MQ=255) aaaCGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGAGAACCAATTCAAGGAAGAAACAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTa < 2:151249/149‑1 (MQ=255) aaCGCCCCTGCTAATGAATATCTGGCATGGTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAAAATTAAAGGAAAAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTAc < 2:599871/149‑1 (MQ=255) aCGCCCCGGTTAATGAATATCTGGCAACTTGTAATACAAGGCCGTTGTTGGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTAcc < 2:276190/149‑1 (MQ=255) cccTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATAACATGTTTa > 1:431522/1‑143 (MQ=255) cccTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTCTTGCTTTGGCAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTa < 1:216461/143‑1 (MQ=255) tatCTGGCATGTTGTACTAAAAGTCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTAc < 2:404091/149‑1 (MQ=255) aacTGGCATGTTGCACTAAAAATCGATGTTTTGCTTCGAAAATCCCCTGGTGTTTTGCGAAAGCATTAAAAAAAAAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGaaa < 1:566873/141‑1 (MQ=255) tgtctgtttgtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTGTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTAt < 2:143172/142‑1 (MQ=255) gttgtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTAt < 1:383729/143‑1 (MQ=255) tAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAGGAAAAAAAACAGTATTCTGATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTgg > 1:479333/1‑143 (MQ=255) aaaaTCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGc > 2:217731/1‑149 (MQ=255) tCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAAGCTACGTATGGCGTGGACAGACGCCAtt > 2:444022/1‑149 (MQ=255) gATGTTTTGCTTTGACAATCCCCTGGGGTTTTGCGAAAACAGTCGAGGAAGAAAAAAAACAGGATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCg < 2:267731/149‑1 (MQ=255) gTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGa < 2:517121/149‑1 (MQ=255) ttttGCTTTGACATTCCCCTGGTGTTTTGCGAAAACATTCGCGGAAGAAAAAAAACAGTCTTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACGGACGCCAtt > 1:117131/1‑143 (MQ=255) ttttGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCAtt > 1:170210/1‑143 (MQ=255) tgtttctctGACAATCACCTGGTGTTTTGCTAAAACAGTCGAGGAAGAAAAAAAACAGTATTCTGATATGCGCATAACCAGGCAGGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCAtt < 1:36890/135‑1 (MQ=255) ttggtatGACAACCCCCTGGTGTTTTGC‑AACACATTCGAGGAAGAAAAAAAACAGTATTCGTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTc < 2:42868/143‑1 (MQ=255) ttGCTTTGACAATCCCCTGGTGTTTTGC‑AAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTc < 2:539292/149‑1 (MQ=255) cTTTGACAATCCCCTGATTTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGa < 2:432104/149‑1 (MQ=255) tGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACGGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGt > 1:282482/1‑143 (MQ=255) ccccTGGGGTTTAGCAAAAACATGCGAGGAAGAAAAAAACCAGTATTCTTATATTCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATTGCGTGGAGAGACGCCATTCGTGATGTCGATAGc < 1:264837/143‑1 (MQ=255) gCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGtct > 2:412379/1‑149 (MQ=255) gAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGGTAGCTGCCACAAGGCAACGGtcttc > 2:326447/1‑149 (MQ=255) gAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGgcttc > 2:326524/1‑149 (MQ=255) | CCCGTCATTATCACCTCTGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGTCTTC > CP009273/927633‑927920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |