Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,515,090 Δ1 bp 11.1% coding (531/789 nt) yjhI ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,515,0890C.11.1% 104.6 / 9.0 36coding (532/789 nt)yjhIputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (17/14);  new base . (2/2);  total (20/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAGTTTTTTGCCA  >  CP009273/4514953‑4515234
                                                                                                                                        |                                                                                                                                                 
gaatagCCAGAAAGTGACCTTGCTTTGTTACCTCCGCTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGCTTGATTCTTCGTTGTTTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAAttt                                                                                                                                       <  2:207813/144‑1 (MQ=255)
   tCGCCAGAAAGTGAGATTGCATAGTTACCTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGATTTTTGTATTTCTTCCAGAAACAAtttttt                                                                                                                                    <  2:332322/149‑1 (MQ=255)
      ccAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTGGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAAAAATTTTTTATc                                                                                                                                 >  2:295402/1‑149 (MQ=255)
      ccAGAAAGTGAGATTGCATAGTTAACAATGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAAttt                                                                                                                                       >  1:556549/1‑143 (MQ=255)
        aGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCAGAAACAATTTTTTATCGGt                                                                                                                              >  2:140029/1‑149 (MQ=255)
        aGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCAGAAACAATTTTTTATCGGt                                                                                                                              >  2:139913/1‑149 (MQ=255)
                     gCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTgcg                                                                                                                  >  2:128671/1‑149 (MQ=255)
                                    ttATACATATTGAATACCGTCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTaa                                                                                                   <  2:131617/149‑1 (MQ=255)
                                       tccctatTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGtt                                                                                                     <  1:125368/139‑1 (MQ=255)
                                       taCATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGtt                                                                                                     <  1:125472/143‑1 (MQ=255)
                                        aCATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGtt                                                                                                     >  1:438380/1‑143 (MQ=255)
                                           tatTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCGTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAAc                                                                                                  >  1:376347/1‑143 (MQ=255)
                                                  tACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTg                                                                                           >  1:105212/1‑143 (MQ=255)
                                                  tACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTa                                                                                     >  2:314855/1‑149 (MQ=255)
                                                      ggCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCAGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTTCGCGTATGTGGAGTTAACGTGAGTGCTTCTaaaaa                                                                                 >  2:308970/1‑149 (MQ=255)
                                                       gCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTaaaaaa                                                                                >  2:327896/1‑149 (MQ=255)
                                                       gCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTaaaaaa                                                                                <  2:183714/149‑1 (MQ=255)
                                                       gCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTaaaaaa                                                                                >  2:10691/1‑149 (MQ=255)
                                                         aTACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAaata                                                                              <  2:494323/149‑1 (MQ=255)
                                                            cTTAAACAGCCCCCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTaaaaa                                                                                 <  1:186957/143‑1 (MQ=255)
                                                             ttAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTCATCATTGTCATTCTTCATGAAACAATTTTTTATCGGTAAAGGTATTGCGCGGATTTGGAGTTAACGTGAGTGCTCCTaaaaac                                                                                >  1:230635/1‑142 (MQ=255)
                                                                 aaaTACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGtt                                                                      <  2:34057/147‑1 (MQ=255)
                                                                   aTACCGCCCCATAGGTTGATTCTTCGTTGTCTAAGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGttct                                                                    >  2:631553/1‑149 (MQ=255)
                                                                         ccccATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGttct                                                                    >  1:393232/1‑143 (MQ=255)
                                                                                 gTTGATTCCTCGTTGTCTAGGGCCCATCCCCGAAGGCGTGTTTTGTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCa                                                      <  2:10008/149‑1 (MQ=255)
                                                                                         ttcGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCtgata                                              >  2:253203/1‑149 (MQ=255)
                                                                                                tCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCtaataa                                             <  1:403416/143‑1 (MQ=255)
                                                                                                 cTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTa                                      >  2:419513/1‑149 (MQ=255)
                                                                                                  tATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTAATTATAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATg                                           <  1:115318/143‑1 (MQ=255)
                                                                                                          aTCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGGGTTAACGTGAGGGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTAccc                                   >  1:144719/1‑143 (MQ=255)
                                                                                                             cccGAAGGCGTGTTTTTTGTAATTCTTGTAGTCACGATGTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGATAATAATGCCTTACCCAAc                                >  1:405606/1‑143 (MQ=255)
                                                                                                             cccGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAAc                                >  1:322894/1‑143 (MQ=255)
                                                                                                               cGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAat                        <  2:611970/149‑1 (MQ=255)
                                                                                                                  aGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAATTAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATg                     <  2:422708/149‑1 (MQ=255)
                                                                                                                     cGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACTTGAGTGCTTCTAAAAAATAATCCAGTGCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAat                        <  1:271772/143‑1 (MQ=255)
                                                                                                                     cGTGTTTATTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCaa                  <  2:170749/149‑1 (MQ=255)
                                                                                                                                    tcttcCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTACTATGCAACTCAAGttt         >  1:199357/1‑143 (MQ=255)
                                                                                                                                    tcttcCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAGTTTTTTGcc   >  2:416713/1‑149 (MQ=255)
                                                                                                                                    tcttcCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAGTTTTTTGcc   >  2:616377/1‑149 (MQ=255)
                                                                                                                                     cttcCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAGTTTTTTGCCa  <  2:564561/149‑1 (MQ=255)
                                                                                                                                      gtcCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTACTTCTGCACTACCCCGCGCGGTCCTACGCAACTCTAGttttt       <  1:480434/142‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                 
GGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAGTTTTTTGCCA  >  CP009273/4514953‑4515234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: