Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 2,400,257 IS5 (+) +4 bp 36.4% intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)9 (0.260) 8/270 NT 29.4% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 2400257 = 21 (0.630)intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 = 2283592NA (NA)15 (0.430) 14/270 NT 40.9% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 21 (0.630)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

TGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCT  >  CP009273/2400109‑2400399
                                                                                                                                                    |                                                                                                                                              
aaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctagaacgaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccc                                                                                                                                                >  2:600400‑M2/149‑149 (MQ=255)
 aaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCt                                                                                                                                               <  2:634123‑M2/2‑1 (MQ=255)
   aactggcgataacgccttaaatggcgaagaaacggtctaaataggctcattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCtag                                                                                                                                             <  2:378257‑M2/4‑2 (MQ=255)
    actggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTaaa                                                                                                                                            <  2:174346‑M2/5‑1 (MQ=255)
      tggggataacgccttaaatggcgaagaaccggtctaaataggctgatccaaggcatttacgggagaaaaaaacggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttacccgcgtattcgcaccttccc                                                                                                                                                >  1:243812‑M2/143‑143 (MQ=255)
      tggggataacgccttaaatcgcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttatgcgcaccttccc                                                                                                                                                >  1:386235‑M2/143‑143 (MQ=255)
       ggggataacgcctcaactggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacaggaagaaaggaaattactgagtcagccgagaagaatttccccgcttattcgcaccttccCt                                                                                                                                               <  1:113083‑M2/2‑1 (MQ=255)
        gggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgcgaagaatttccccgcttattcgcacattccCTa                                                                                                                                              >  1:551897‑M2/141‑143 (MQ=255)
         ggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcgCTaa                                                                                                                                             <  1:236402‑M2/4‑1 (MQ=255)
         ggataacgccttaaagggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTaa                                                                                                                                             <  1:118617‑M2/4‑1 (MQ=255)
          gngaacgccgtaagtggcgaagaaacggtgtaaataggctgattcaaggcatttacgggagaaaaaagcggcgcaaacatgaagaaatgaaatgactgagtcagccgagaagaatctccccgcttattcgcaccttccCTaaa                                                                                                                                            <  1:476778‑M2/5‑1 (MQ=255)
           tatatTATCACTGACTGGCGGCTCAGTCTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTaaaa                                                                                                                                           <  1:414678/143‑1 (MQ=255)
             tatgatCACTGACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATGCGGAACCCGGACGAAAGTAAAGATGCATATGAGTTGCACTaaaaaa                                                                                                                                         <  1:21358/143‑1 (MQ=255)
             aacgccttaaatggcgaagaaacggtctaaataggctgattaaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagcagagaagactttccccgcttattcgcaccttacctagtttt                                                                                                                                         >  1:412969‑M2/136‑138 (MQ=255)
              attatCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTTCACTAAAAAAGCGACTc                                                                                                                                  >  2:74873/1‑149 (MQ=255)
              acgccttaaatggcgaagaaaccggctaaataggcttattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAg                                                                                                                                        <  1:264746‑M2/9‑1 (MQ=255)
               cgccttaaatggcgaagaaacggactaaataggctgattcaaggcaaaaaagagaaaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGc                                                                                                                                       <  1:35552‑M2/10‑1 (MQ=255)
                  tCACTTACGGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaa                                                                                                                              >  2:180675‑M2/1‑134 (MQ=255)
                   cACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGATTTGCACTAAAAAAGCGACTCACAtt                                                                                                                             <  2:381239/149‑1 (MQ=255)
                    taaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaacaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaacaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTg                                                                                                                            <  2:357345‑M2/21‑1 (MQ=255)
                    aCTTACTGGCGGCTCATACGGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGAAAAAAAAAAGCGACTc                                                                                                                                  <  1:577640/143‑1 (MQ=255)
                            ccagctcagtatGAGCCGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTACACTAAAAAAGCGACTCACAGTGTTCCGttc                                                                                                                    >  2:353643/12‑148 (MQ=255)
                              tgcttctACTGAGCTGGTTAATGCTGTGCACACACAAACAAGTAATTCCTTTTGTAATGTGCCTTCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTtata                                                                                                                  <  2:10880/143‑1 (MQ=255)
                              ggcTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatg                                                                                                                  <  2:565979‑M2/149‑28 (MQ=255)
                                   aTACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGcc                                                                                                             >  2:629126/1‑149 (MQ=255)
                                    ggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaaggaaatgactgagccagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTtata                                                                                                                  >  1:269042‑M2/113‑143 (MQ=255)
                                        gAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagat                                                                                                              >  1:82744‑M2/1‑112 (MQ=255)
                                         aaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGt                                                                                                       <  2:553339‑M2/42‑1 (MQ=255)
                                                         gCACACACCAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctgga                                                                                             >  1:152363‑M2/1‑95 (MQ=255)
                                                          cacacacaAGCTAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatag                                                                                      >  2:347778‑M2/1‑94 (MQ=255)
                                                          cacacacaAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatag                                                                                      >  2:347807‑M2/1‑94 (MQ=255)
                                                           acacacaAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAATTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACGAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccataga                                                                                     <  2:528057‑M2/149‑57 (MQ=255)
                                                            cacacaAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAata                                                                                          >  1:359954/1‑143 (MQ=255)
                                                                 aaacaaGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAatatcact                                                                                     >  1:59005/1‑139 (MQ=255)
                                                                      gaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAgg                                                                                >  1:393563‑M2/79‑143 (MQ=255)
                                                                         aaTTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggtt                                                                             >  1:510396‑M2/1‑79 (MQ=255)
                                                                               aTTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgaggacaggtttggcagctggagccatagaacagggttcatcat                                                                       <  1:400759‑M2/143‑71 (MQ=255)
                                                                                 ttGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccgtagaacagggttcatcatga                                                                     <  1:369394‑M2/143‑73 (MQ=255)
                                                                                      acgggcgcccctgggagttTCATCCGGAACCCGGACGAAAGGAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcaggtttgtcatctggagccatagaacagggttcatcatgagtcat                                                                >  1:458089‑M2/9‑66 (MQ=255)
                                                                                             tCCCTGGCCGTGTCATCCGGAACCCGAACGAAAGTAAAAATGAAGATAAGTAGCAATAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCTCCCAATCGATCTTCAGGGATCGCATATCTATTAAGTTcct                                                         <  1:376632/143‑1 (MQ=255)
                                                                                             tCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCAATTAAGTTACTCActct                                                   >  2:603487/1‑149 (MQ=255)
                                                                                               ccTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATACTGCCTGAAGTAGATCAAAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCttt                                                 <  2:91428/149‑1 (MQ=255)
                                                                                                cTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCCCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccg                                                <  2:43774‑M2/149‑94 (MQ=255)
                                                                                                       tttCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGCATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTc                                               >  1:174331/1‑143 (MQ=255)
                                                                                                        ttCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcgtctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaa                                        >  2:423667‑M2/1‑48 (MQ=255)
                                                                                                           aTCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGAc                                     <  2:359833/149‑1 (MQ=255)
                                                                                                               ggAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgccaactattccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgaaagtgaattcagca                                 >  2:435642‑M2/1‑41 (MQ=255)
                                                                                                               cagccgagaagaatttccccgcttattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATg                                       <  1:164426‑M2/106‑1 (MQ=255)
                                                                                                                   cccGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCgt                             <  2:612510/149‑1 (MQ=255)
                                                                                                                    ccGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATg                                  >  1:324383/1‑143 (MQ=255)
                                                                                                                     cGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATgc                                 <  1:569743/143‑1 (MQ=255)
                                                                                                                     cGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCgtgt                           >  2:374116/1‑149 (MQ=255)
                                                                                                                         cGAAAGTAAAAATGCATCTGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAACGCCTGAAGTAGATCACAGAATATATCTTCAGGGATAGCATATCTATTAAGTTACTCACTGTTTTCTATTTATGACATGCGCgt                             >  1:421359/1‑143 (MQ=255)
                                                                                                                          gAAAGTAAAAATGCATATGAGTTGCACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgt                      >  2:128739‑M2/1‑30 (MQ=255)
                                                                                                                               atcagataattcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGGCTTTGt                       >  1:444539‑M2/22‑143 (MQ=255)
                                                                                                                                  aaaTGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGGGTTTGTAtaaatgta              >  2:83081/1‑149 (MQ=255)
                                                                                                                                     ttagtcgcaccttccCTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGTATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaatgtaaat           <  2:610218‑M2/134‑1 (MQ=255)
                                                                                                                                     tGCCTATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTAtaaat                 <  1:312780/143‑1 (MQ=255)
                                                                                                                                       cATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTCCAGGGATCGCATATCTATTAAGTTACTCACTCTTATCTCTTATGGACTTGCGCGTGTTTGCATaaatgtaaatgt         <  2:470135/149‑1 (MQ=255)
                                                                                                                                                   aCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgcgtcatcaacttaccttcgccgacagagaattcagcagtaagcgccgtcagaccagaaaagagattt   <  1:308739‑M2/143‑139 (MQ=255)
                                                                                                                                                    cTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaacgaggtttt  <  1:154603‑M2/143‑140 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
TGCACTTATCATATATTATCACTTACTGGCGGCTCATACTGAGCTGGTTAATGCTGTGCACACACAAACAAGCAATTCCATTTGTAATGTGCCTCCCTGGCAGTTTCATCCGGAACCCGGACGAAAGTAAAAATGCATATGAGTTGCACTAAAAAAGCGACTCACATTGTTCCGTTATAATGCCTGAAGTAGATCACAGAATATATCTTCAGGGATCGCATATCTATTAAGTTACTCACTCTTTTCTATTTATGACATGCGCGTGTTTGTATAAATGTAAATGTGAGTCCT  >  CP009273/2400109‑2400399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.