| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,420,353 | A→C | 22.7% | intergenic (‑86/+135) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,420,353 | 0 | A | C | 22.7% | 42.8 / 11.6 | 31 | intergenic (‑86/+135) | hisJ/argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (15/8); new base C (4/3); total (20/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.50e-01 | |||||||||||
AACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCA > CP009273/2420206‑2420494 | aaCGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCaaaa < 2:577414/149‑1 (MQ=255) ttGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAGAATTGACCATCAAGGCAAAATATCCCGccc > 2:213006/1‑149 (MQ=255) ttGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATAGTGCCATAAAATTGACCATCCAGGCAAAATATc > 1:288468/1‑143 (MQ=255) cTGGAGAAGGCCAGAACCAGAGAGAGTGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTa < 2:98513/149‑1 (MQ=255) ccAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAACGTTTACAGGTTTGTTAAACTTATCGTGCCATAAAATTGACCACCAAGACAAAATATCCCGCCCCGCTAGACg > 1:423280/1‑143 (MQ=255) cAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACCCTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGCTAGACGt > 1:55966/1‑143 (MQ=255) cAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTattatt > 1:153986/1‑143 (MQ=255) ggagcgCGATAGCATCAGTTTTTGCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTAttttt < 1:248655/142‑1 (MQ=255) agagagCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTaa > 2:554036/1‑149 (MQ=255) gagCGATAGCACCAGGTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGCCCATCAAGACAAAATATCCCGCCCCGTTAAACGTCTTATTTTTCAt > 1:332224/1‑143 (MQ=255) cGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTCGAGGTTTGTTAAGCTTATCGTGACATACACCCGAAAATCCAGGAAGGATATACCGCTCCGGCAGACGCATTATTTTTCACTaa > 1:190477/1‑143 (MQ=255) gATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAATCATTTTATACGGAATATTTACATGTTTGTTACACTTACCGTGCCATAAAAGTGACCATCAAGGCAAAATCTCCCGCCCCGTTTGACGTATTATTTTTCATTAAATATGCt < 2:252491/149‑1 (MQ=255) gATAGCACCAGTTTTTTCATAATGTCCTCAACTAACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCt > 2:329760/1‑149 (MQ=255) aTAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCtt > 2:543919/1‑149 (MQ=255) gccAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCt > 1:300534/2‑143 (MQ=255) cAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAAAGCTTAATTATg > 2:108907/1‑149 (MQ=255) aaCTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGcacca > 1:70984/1‑143 (MQ=255) tCTTTTTATAGGGAATATTTACCGGTTTGTTACACTTATCGTGCCATAAAATTGACCATAAAGGCAAAATATCCCGCCCCGATAGTCGTATTATTTTTCATTAAAAATGCTTAAGTATGCCGTTCAATGCCCCACGATAGTGc > 1:122570/1‑143 (MQ=255) aGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTg < 2:214068/149‑1 (MQ=255) aaTATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCAcc > 2:255677/1‑149 (MQ=255) tatTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGCCGTg > 1:133707/1‑143 (MQ=255) tttACGGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCatga < 2:635195/149‑1 (MQ=255) tttACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCatga < 2:635194/149‑1 (MQ=255) aGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATc < 2:346539/149‑1 (MQ=255) aGGTTTGTTACACTTATCGTGCAATAAAATTGACCATCAAGGCAAAATATCCCGCCTCGTTAGACGTATTATTCTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATc < 2:252649/149‑1 (MQ=255) tACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATc < 2:111209/149‑1 (MQ=255) acTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAgg > 2:12180/1‑149 (MQ=255) gCCATAAAATTGACCATCAAGGCAAAATAGCCCGCCCCGTTAGACGTCTTATTTTTCATTAAAAATGCTTAAGTATGCATTTCGATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATTATCATTCCATCAGGTAc < 1:422272/143‑1 (MQ=255) tAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATAAGGTACAGCt > 1:26965/1‑143 (MQ=255) gACAATTAAGGAAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTa < 2:267590/149‑1 (MQ=255) aCCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTAGCGTGAAGTGTGGGGATCATTCCATCAGGTACAGCTTCGCAGCGACGGAt > 2:276761/1‑149 (MQ=255) aaGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCa > 2:445448/1‑149 (MQ=255) | AACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCA > CP009273/2420206‑2420494 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |