| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,420,394 | G→T | 33.4% | intergenic (‑127/+94) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,420,394 | 0 | G | T | 33.4% | 26.2 / 23.8 | 33 | intergenic (‑127/+94) | hisJ/argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (11/11); new base T (5/6); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01 | |||||||||||
GATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTC > CP009273/2420248‑2420535 | gATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAATCATTTTATACGGAATATTTACATGTTTGTTACACTTACCGTGCCATAAAAGTGACCATCAAGGCAAAATCTCCCGCCCCGTTTGACGTATTATTTTTCATTAAATATGCt < 2:252491/149‑1 (MQ=255)gATAGCACCAGTTTTTTCATAATGTCCTCAACTAACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCt > 2:329760/1‑149 (MQ=255) aTAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCtt > 2:543919/1‑149 (MQ=255) gccAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCt > 1:300534/2‑143 (MQ=255) cAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAAAGCTTAATTATg > 2:108907/1‑149 (MQ=255) aaCTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGcacca > 1:70984/1‑143 (MQ=255) tCTTTTTATAGGGAATATTTACCGGTTTGTTACACTTATCGTGCCATAAAATTGACCATAAAGGCAAAATATCCCGCCCCGATAGTCGTATTATTTTTCATTAAAAATGCTTAAGTATGCCGTTCAATGCCCCACGATAGTGc > 1:122570/1‑143 (MQ=255) aGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTg < 2:214068/149‑1 (MQ=255) aaTATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCAcc > 2:255677/1‑149 (MQ=255) tatTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGCCGTg > 1:133707/1‑143 (MQ=255) tttACGGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCatga < 2:635195/149‑1 (MQ=255) tttACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCatga < 2:635194/149‑1 (MQ=255) aGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATc < 2:346539/149‑1 (MQ=255) aGGTTTGTTACACTTATCGTGCAATAAAATTGACCATCAAGGCAAAATATCCCGCCTCGTTAGACGTATTATTCTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATc < 2:252649/149‑1 (MQ=255) tACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATc < 2:111209/149‑1 (MQ=255) acTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAgg > 2:12180/1‑149 (MQ=255) gCCATAAAATTGACCATCAAGGCAAAATAGCCCGCCCCGTTAGACGTCTTATTTTTCATTAAAAATGCTTAAGTATGCATTTCGATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATTATCATTCCATCAGGTAc < 1:422272/143‑1 (MQ=255) tAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATAAGGTACAGCt > 1:26965/1‑143 (MQ=255) gACAATTAAGGAAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTa < 2:267590/149‑1 (MQ=255) aCCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTAGCGTGAAGTGTGGGGATCATTCCATCAGGTACAGCTTCGCAGCGACGGAt > 2:276761/1‑149 (MQ=255) aaGGCAACATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCa > 2:445448/1‑149 (MQ=255) acatatCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCGTCTACCCATACGTGCACCATGATGATCATTTAATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAg < 2:597372/147‑1 (MQ=255) cGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGa > 2:381491/1‑149 (MQ=255) aCGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCCCGATAGTGCAACAAACCTGGCGAGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTACCAGTCCCCGTAGACATTAAACTCGAAATACt > 2:536164/1‑149 (MQ=255) cGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACtt < 2:156048/149‑1 (MQ=255) tattatTTTTCATTAAAAATGCTTAAGTATGCGTTTCAATGCACGACGATAGTGCAACAAACCTGGTGTGCACCATGATGATCATTCCATCAGGTACTGCTTCCCAGCGACATATCAGTCACCGTAGCCATAAAAGACGAAATACttac > 2:479607/1‑147 (MQ=255) tttttCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACATACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGcc < 2:340615/149‑1 (MQ=255) ttgttCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACttt < 1:97577/140‑1 (MQ=255) aTTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATCCTTTTTCgac > 1:432438/1‑141 (MQ=255) aTTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTg < 2:179384/149‑1 (MQ=255) tAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCAt < 1:197511/143‑1 (MQ=255) gaaacaTGCTTAAGTATGCATTTCAACGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTc < 2:374887/148‑1 (MQ=255) tGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTc < 1:132479/143‑1 (MQ=255) | GATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTC > CP009273/2420248‑2420535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |