| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,854,442 | A→G | 11.8% | L51P (CTG→CCG) | ygbI ← | DeoR family putative transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,854,442 | 0 | A | G | 11.8% | 78.3 / 4.7 | 34 | L51P (CTG→CCG) | ygbI | DeoR family putative transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (15/15); new base G (2/2); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.04e-01 | |||||||||||
ATACAACTTCCCGGCTGAACAAGACTTGCCGCCAGCTTGCCAATAGCCGCTTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTA > CP009273/2854307‑2854583 | ataCAACTTCCCGGCTGAACAAGACTTGCCGCCAGCTTGCCAATAGCCGCTTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTcc < 2:99977/149‑1 (MQ=255) acactttCCCGGCTGACAAAGACTTGCCGCCGGCTTGCCAATAGCCGCTTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACgg < 1:22335/138‑1 (MQ=255) aCTTCCCGGCTGAACAAGACTTGCCGCCAGCTTGCCAATAGCCGCTTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTc < 1:85171/143‑1 (MQ=255) cAAGACTTGCCGCCAGCTTGCCAATAGCCGCTTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGt > 1:499032/1‑143 (MQ=255) aCTTGCCGCCAGCTTTCCAATAGCCGCTTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGttttt < 1:413196/143‑1 (MQ=255) aCTTGCCGCCAGCTTGCCAATAGCCGCTTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCGGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGttttt > 1:188266/1‑143 (MQ=255) aCTCGCCGCCAGCTTGCCAATAGCCGCGTTTCGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGttttt < 1:413220/143‑1 (MQ=255) tGCCCCCAGCTTGCCAATAGCCGCTTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTa < 1:146481/143‑1 (MQ=255) gccgccAGCTTGCCAATAGCCGCTTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCGGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTaa > 1:314379/1‑143 (MQ=255) gccgccAGCTTGCCAATAGCCGCTTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCGGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTaa > 1:314414/1‑143 (MQ=255) ttGCCAATAGCCGCTTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCcgacg > 1:565909/1‑143 (MQ=255) cAATAGCCGCGTTTTGCGTCATGGCCAGCGCAGTCTTTGCCTGATGAGAAGTTTCATGCGCCACGCGTCCCGGAGACTGGACGCCCCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCata < 2:513268/149‑1 (MQ=255) actggCCGCGTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTACATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTATGTAAATCCCGACGAATGGTCatat < 2:379339/145‑1 (MQ=255) attgCCGCTTTTGGCGTCATTGCCCGCGCAGTTTTTACCTGCTGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGt < 1:157879/140‑1 (MQ=255) gCTTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGCTGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGGCGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAACTCCCGAACAATGGTCataag > 1:162960/1‑141 (MQ=255) cTTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGAcaca > 2:249285/1‑149 (MQ=255) cATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGt < 2:166658/149‑1 (MQ=255) cAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTa > 2:60076/1‑149 (MQ=255) gcAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTc > 2:17597/1‑149 (MQ=255) tgaCCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATa < 2:521214/147‑1 (MQ=255) cTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGTCTGGACGCCTCCGGACACCGGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGa < 2:355349/149‑1 (MQ=255) cTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGTCTGGACGCCTCCGGACACCGGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATCCTGTCCGTTAGTTCAGCAATACTGa < 2:355385/149‑1 (MQ=255) gatgaGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACa < 2:121349/149‑1 (MQ=255) gagaAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAAAACTg > 1:466494/1‑143 (MQ=255) gTTCATGCGCCACGCGTCCCGGAGCCTGGACGCCTCCGGACACCAGCACAAAGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGCCGAATGGTCATATGTGAAACATTCATTCTGTCCGTTAGTTCAGCAATACTGAAAATg > 1:232887/1‑143 (MQ=255) ttCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGc < 1:15434/143‑1 (MQ=255) aTGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCtt > 1:499795/1‑143 (MQ=255) cACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCCGTTTTTGTAAATCCCGACGAATGGTAATATGTGACACATTCATTCTGTCCGTTAGTTCAGGAATACTGACAATGCCTTTTTCTGCTACCa > 2:17855/1‑149 (MQ=255) ccGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGGTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTACGTTAGTTCAGCAATACTGAGAATGCCTTTTTTAGCTACCAtc > 1:154792/1‑143 (MQ=255) tGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 2:90818/1‑149 (MQ=255) ggACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTgg < 2:169037/149‑1 (MQ=255) ggACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTgg < 2:128463/149‑1 (MQ=255) gACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGTCGACg > 1:297362/1‑143 (MQ=255) cAGCACAACGGCTCCCTGCTGCGCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCATCTACCATCTCCAGGATGATTTCGCGACGCTCTa > 1:358843/1‑143 (MQ=255) | ATACAACTTCCCGGCTGAACAAGACTTGCCGCCAGCTTGCCAATAGCCGCTTTTTGCGTCATTGCCAGCGCAGTTTTTACCTGATGAGAAGGTTCATGCGCCACGCGTCCCGGAGACTGGACGCCTCCGGACACCAGCACAACGGCTCCCTGCTGCTCCAGTTTTTGTAAATCCCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTA > CP009273/2854307‑2854583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |