| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,855,442 | T→G | 10.3% | pseudogene (1522/1617 nt) | glvC ← | pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,855,442 | 0 | T | G | 10.3% | 132.3 / 4.3 | 53 | pseudogene (1522/1617 nt) | glvC | pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component |
| Reads supporting (aligned to +/- strand): ref base T (20/27); new base G (2/4); total (22/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGGTGCTTCCACCGCCTGCGACAACCACTGAGAATTTGGTCATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCC > CP009273/3855304‑3855588 | aaGGTGCTTCCACCGCCTGCGACAACCACTGAGAATTTGGTCATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAAGGATCACCTGa > 2:574738/1‑149 (MQ=255)aaGGTGCTTCCACCGCCTGCGACAACCACTGAGAATTTGGTCATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTCAGATACATGCAGAACAAGGaac > 1:117524/1‑141 (MQ=255) ttCCACCGCCTGCGACAACCACTGAGAATTTGGTCATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAAGTAAGCTATCGAGCTGCTCGCGGCGCTGGGATACATGCAGACCAATGATCACCTGAATGGCAt > 2:432804/1‑149 (MQ=255) ttCCACCGCCTGCGACAACCACTGAGAATTTGGTCATTATACTGCCTCCGTAATGGCAACAGTTTCTGCTGATGGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGaa < 1:56915/143‑1 (MQ=255) tCCACCGCCTGCGACAACCACTGAGAATTTGGTCATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGGTCCCCTGAAt > 1:452997/1‑143 (MQ=255) tCCACCGCCTGCGACAACCACTGAGAATTTGGTCATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAAt < 1:378049/143‑1 (MQ=255) ccGCCTGCGACAACCACTGAGAATTTGGTCATTATTCTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCa < 2:157118/149‑1 (MQ=255) gCCTGCGACAACCACTGAGAATTTGGTCATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCCCCTGAATGGCATCGCCACt > 2:209894/1‑149 (MQ=255) tGCGACAACCACTGAGAATTTGGTCATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATCAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGcc < 1:467126/143‑1 (MQ=255) gaaaaCCACTGAGAATTTGGTCATTATACTGCCGCCGTAATGGCAACATTTTCTGCTGATTGATGAGAAGTAATTAATCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGTATggattagcagta < 1:210224/140‑11 (MQ=255) gACAACCACTGAGAATTTGGTCATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGaa < 2:185488/149‑1 (MQ=255) gACAACCACTGAGAATTTGGTCATTATACTGCCTCAGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATAATCACCTGAATGGCATCGCCACTATGGaa < 2:386989/149‑1 (MQ=255) cAACCACGGAGAATTTGGTAATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGa < 2:168448/149‑1 (MQ=255) aCCACTGAGAATTTGGTCAGTATACTCCCTCCGTAATAGCAACCTTTTCTGCTGATTGATGAGGATTAATTTAGCTATCGAGCAGTTCACGCAGCTGAGATACAAGCAGACCAATGCTCACCTGAATAGCCTCGCCACTACgg > 1:270155/1‑143 (MQ=255) aCTGAGAATTTGGTCATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCg < 2:263534/149‑1 (MQ=255) tGAGAATTTGGTCATTATACTGCCTCCGTAAAGAACAGAATATCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCCCCTGAATGGCATCGCCACTACGGACGACGCCGTg > 2:630713/1‑149 (MQ=255) gagaAGTGGGTCATTATACTGCCTCCGTAGTGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAAGGATCACCTGAATGGCATCGCCAATACGGAAGACg < 1:119848/143‑1 (MQ=255) tttGGTCATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCTGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTcc < 2:439024/149‑1 (MQ=255) ggTCATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAg > 2:97080/1‑149 (MQ=255) gTCATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGc > 2:42560/1‑149 (MQ=255) tGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGa < 2:179235/149‑1 (MQ=255) cgccGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGTTTTTTaa < 1:387248/141‑1 (MQ=255) cTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACtt < 2:556495/149‑1 (MQ=255) cAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGc < 2:292295/149‑1 (MQ=255) aaCATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCGACTACGGAAGACGCCGTGCGCTCCCGACTGGTGAAATACTTCGtant > 1:521390/1‑140 (MQ=255) ccttgtcggcggcttgagGGGAATTAATGGAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCGGAATGGCATCGCCACTACGGAAGACGCCGTGCGCCCCCAGCTTTTTAAAGACTTCGTCATCCAGCGtt < 2:541002/136‑1 (MQ=255) cATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGtt < 2:595222/149‑1 (MQ=255) ctgctgATTGATGAGAATTAATTAAGCTATCGAGCAGTTTACGCATCTTAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGAc < 2:432703/149‑1 (MQ=255) gctgATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGCCGCCGTGCGCTCCCAGCTTTTTAAAGCCTTCGTCATCCAGCGGTtg > 1:487638/1‑143 (MQ=255) tatctccagaATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGAc > 1:341153/8‑143 (MQ=255) tgatgaGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAAGGTTCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATg < 2:131027/149‑1 (MQ=255) tgatgaGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAAGGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATg < 2:131048/149‑1 (MQ=255) atgaGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCGGCGTTTGTGACATGt > 1:193270/1‑143 (MQ=255) gaATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAAGGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATg < 1:518689/143‑1 (MQ=255) attaattaAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCa < 1:253975/143‑1 (MQ=255) cTATCGAGCTGGTCCCGCAGCGGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAAcg < 2:146627/149‑1 (MQ=255) tATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCCATACGt > 1:511484/1‑143 (MQ=255) gAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTcg > 2:365623/1‑149 (MQ=255) gtcTGGTCACGAAGCTGAGCTACATGCAGACCAATGATCCCCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAAcg < 1:335963/141‑1 (MQ=255) aGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCGGCGTTTGTGACATGTCATGCAGTGCAATACTTAAAAGCGTcgc > 2:2732/1‑149 (MQ=255) aGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGATTGTGACATGTCATGCAGTGCAATACGtcaccc > 1:246602/1‑138 (MQ=255) cTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGCCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCgcgc < 2:306999/149‑1 (MQ=255) ttCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAAt > 2:637026/1‑149 (MQ=255) aCGCAGCTGAGATAAATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCACTGCAATACGTAAACGCGTCGCGCAATTGt < 2:400238/149‑1 (MQ=255) tGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCt > 2:182429/1‑149 (MQ=255) cATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGata < 2:384555/149‑1 (MQ=255) aTGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTgg > 1:181933/1‑143 (MQ=255) aGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGcc > 2:553241/1‑149 (MQ=255) gACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGata < 1:86919/143‑1 (MQ=255) ccAATGATCACCTGAATGGCATCGCCACTACGGACGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGAc < 2:88180/149‑1 (MQ=255) cAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGAcc > 2:636637/1‑149 (MQ=255) cAATGATCACCTGAACGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTg < 1:186833/143‑1 (MQ=255) aaTGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGAccc < 2:396975/149‑1 (MQ=255) | AAGGTGCTTCCACCGCCTGCGACAACCACTGAGAATTTGGTCATTATACTGCCTCCGTAATGGCAACATTTTCTGCTGATTGATGAGAATTAATTAAGCTATCGAGCTGTTCACGCAGCTGAGATACATGCAGACCAATGATCACCTGAATGGCATCGCCACTACGGAAGACGCCGTGCGCTCCCAGCTTTTTAAAGACTTCGTCATCCAGCGTTTGTGACATGTCATGCAGTGCAATACGTAAACGCGTCGCGCAATTGTTAATGCTGGAGATATTGCCGACCC > CP009273/3855304‑3855588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |