Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	CP009273	2,160,620	IS5 (+) +4 bp	intergenic (+110/+160)	yegQ → / ← ogrK	putative peptidase/positive regulator of P2 growth (insertion of P2 ogr gene into the chromosome)

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	2095230 =	NA (NA)	54 (0.990)	42/292	0.3	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	CP009273	2160620 =	0 (0.000)					intergenic (+110/+163)	yegQ/ogrK	putative peptidase/positive regulator of P2 growth (insertion of P2 ogr gene into the chromosome)
*	?	CP009273	= 2096424	NA (NA)	40 (0.730)	38/292	0.5	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	CP009273	= 2160623	0 (0.000)					intergenic (+113/+160)	yegQ/ogrK	putative peptidase/positive regulator of P2 growth (insertion of P2 ogr gene into the chromosome)

AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/2096283‑2096424 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAATTTTCCATCCGGGAAAAATAATCGAAATTAATCACTTACCGTGGGGATTACGCGTGGTTTCCCCGG  <  CP009273/2160623‑2160476                                                                                                                                                                                                                                                                                                     AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCT                                                                                                                                                     <  2:721524/144‑1    AAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTT                                                                                                                                             >  1:660023/1‑149       cTCTCGTTTCTGGTCTGACGGCGCCTACTGCTGAATTCACTGTCGGCGCAGGGAATTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATG                                                                                                                                          <  1:584357/148‑1       ATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATG                                                                                                                                          <  1:109320/149‑1        TCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGG                                                                                                                                         <  1:65431/149‑1             TTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTCTGGGTTAT                                                                                                                                    >  1:307156/1‑149                      TGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAG                                                                                                                          <  1:197681/149‑1                      TGACGGCGCTTACTGCTGAATTCACTGGCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TT                                                                                                                               <  2:441524/144‑1                        gCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTTATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTT                                                                                                                             <  2:57186/143‑1                              CTTACTGCTCAATTCACTGCTGGCGAAGGTAAGTTGATGACTCATGATGCACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGA                                                                                                                       <  2:39998/144‑1                                     CTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAA                                                                                                           >  1:192301/1‑149                                     CTGAACTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGAT                                                                                                                <  2:567761/144‑1                                      TGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAAC                                                                                                          <  1:734261/149‑1                                          TTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAA                                                                                                           >  2:229784/1‑144                                                 TCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAAT                                                                                                    <  2:854467/144‑1                                                 TCGGCGAAGGTAAATTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCACTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAAT                                                                                                    >  2:511502/1‑144                                                    GCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAA                                                                                                 >  2:523240/1‑144                                                         GGTAATGTGATGATCCATGATGAACCCTGTCATATGGCTCCACTTGACAAACATGATCGCATATCAGGGACGTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAG                                                                                            <  2:834273/144‑1                                                         GGTAAGTTGATTACTCATGATTAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGTATTATTTGC‑AATCGGATGATAACCTTAATAAATGCAG                                                                                            <  2:600971/144‑1                                                          GTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAAACTTAATAAAGGCAGCTGTAT                                                                                      >  1:478530/1‑149                                                                       TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGG                                                                              >  2:849738/1‑144                                                                         ATGATGAACCCTGTCCTATGGCTCCAGATGTCAATCATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAA                                                                       <  1:471727/149‑1                                                                            ATGAACCCTGTTCTATAGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGGTTCTAAGAA                                                                    >  1:584929/1‑149                                                                               AACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAG                                                                      >  2:36678/1‑144                                                                                 CCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAA                                                                    <  2:175423/144‑1                                                                                     GTTCTATGGCTCCAGATGACAAACATGATCTTATATCAGGGCCTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAATTTTCCATC                                                           <  1:353800/149‑1                                                                                      TTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAATTTTCCATCC                                                          >  1:129614/1‑149                                                                                           TGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAATTTTCCATCCGGGAA                                                     <  1:321069/149‑1                                                                                             GCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAATTTTCCATCCGGGAAAA                                                   >  1:763551/1‑149                                                                                                  AGATGACAAACATGGTCTCATATCAGGGACTGGTTCGCACCTTCCCTAGCATTTTATAGGTTATGT‑TTTTCAGCGAAACGGATGATAACATTAATAAATGCAGATGTATGTGATCGGTTTCTAAGAATTTTCCATCCGGGAAAA                                                   >  2:430001/1‑144                                                                                                      GACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAATTTTCCATCAGGGAAAAATAA                                               >  2:564348/1‑144                                                                                                        CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTAGGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATGGGTTTCTAAGAATTTTCCATCCGGGAAAAATGATCGAAgt                                        >  1:1183/1‑147                                                                                                         AAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAATTTTCCATCCGGGAAAAATAATCGAAATT                                       >  1:583224/1‑149                                                                                                           ACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAATTTTCCATCCGGGAAAAATAATCGAA                                          <  2:429427/144‑1                                                                                                                   TCATATCAGGGACTTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAATTTTCCATCCGGGAAAAATAATCGAAATTAATCA                                  >  2:109366/1‑144                                                                                                                                TTGTTCGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAATTTTCCATCCGGGAAAAATAATCGAAATTAATCACTTACCGTGGGGA                     >  2:610965/1‑144                                                                                                                                     CGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAATTTTCCATCCGGGAAAAATAATCGAAATTAATCACTTACCGTGGGGATTACGCTTGG           >  1:382563/1‑149                                                                                                                                     CGCACCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAATTTTCCATCCGGGAAAAATAATCGAAATTAATCACTTACCGTGGGGATTACGCGTGG           <  1:801902/149‑1                                                                                                                                         CCTTCCCTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAATTTTCCATCCGGGAAAAATAATCGAAATTAATCACTTACCGTGGGGATTACGCGTG            <  2:790642/144‑1                                                                                                                                              CCTAGCATTTTATGGCTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGCTCGGTTTCTAAGAATTTTCCATCCGGGAAAAATAATCGGCAATCTGCACTTACCGTGGGGATTACGCGTGGTTTCCCCGG  <  1:224332/149‑1                                                                                                                                                                                                                                                                                                     AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/2096283‑2096424 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAGCATTTTATGGGTTATGT‑TTTTCAGCGAAACGGATGATAACCTTAATAAATGCAGCTGTATGTGATCGGTTTCTAAGAATTTTCCATCCGGGAAAAATAATCGAAATTAATCACTTACCGTGGGGATTACGCGTGGTTTCCCCGG  <  CP009273/2160623‑2160476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.