Predicted mutation
evidence seq id position mutation annotation gene description
MC JC CP009273 4,145,501 Δ320 bp coding (573‑892/1005 nt) argC → N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 4145501 4145820 320 61 [0] [0] 61 argC N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 41455000 (0.000)57 (1.090) 45/278 0.2 100% coding (572/1005 nt) argC N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding
?CP009273 4145821 = 0 (0.000)coding (893/1005 nt) argC N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding

TCGGGTGTGACCCAGGCGCAAGTCGCGCAAGTGTTACAGCAGGCGTATGCCCATAAACCGCTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTG  >  CP009273/4145673‑4145967
                                                                                                                                                    |                                                                                                                                                  
aattaaaagaagcgaatttgattgcggtgccgggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcga                                                                                                                                                    <  1:870824‑M2/1‑1 (MQ=255)
  ttaaaagaagcgaatttgattgcggtgccgggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccgggtgtctacgccaccagcggcgtgagcggtgcagggcgAGc                                                                                                                                                  >  1:630208‑M2/147‑149 (MQ=255)
   taaaagaagcgaatttgattgcggtgccgggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCa                                                                                                                                                 >  1:129003‑M2/146‑149 (MQ=255)
       agaagcggattggagggcggggccggggtggtatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaagcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGc                                                                                                                                                  <  2:797298‑M2/3‑1 (MQ=255)
            cgaatttgattgcggtgccgggctgttaccagacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGattat                                                                                                                                        <  1:863528‑M2/13‑1 (MQ=255)
                tttgattgcggtgccgggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATtgtg                                                                                                                                    <  1:539012‑M2/17‑1 (MQ=255)
                tttgattccggtgccgggctgttatccgacggtggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATtgtg                                                                                                                                    <  1:474031‑M2/17‑1 (MQ=255)
                   gattgcggtgccgggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGCTTATtg                                                                                                                                      <  2:542065‑M2/15‑1 (MQ=255)
                     ttgcggtgccaggctgtaaaccgaaagcggaacagctggagctaaaaccattgattgatgccgatcttcttgacctcactcagtggccggtgatcaacgccaccagcggcgtgagctgtgcagggcgAGCATCTGATTATTGTGGCGAc                                                                                                                               <  1:886973‑M2/22‑1 (MQ=255)
                            gccgggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCg                                                                                                                             <  2:314064‑M2/24‑1 (MQ=255)
                             tcgggctgttatcggacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACa                                                                                                                       <  1:53500‑M2/30‑1 (MQ=255)
                                ggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGa                                                                                                                         <  2:315023‑M2/28‑1 (MQ=255)
                                ggctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACt                                                                                                                    <  1:128456‑M2/33‑1 (MQ=255)
                                 gctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGAc                                                                                                                        <  2:263625‑M2/29‑1 (MQ=255)
                                  ctgttatccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgccctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAAATTa                                                                                                                  >  1:243885‑M2/115‑149 (MQ=255)
                                   tgttatccgacggcggcacagctggcgcggaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatccacgcaacgagcggcatgcatgatccagggcgAGCATCTGATTATTGTGTCGACCGAAGACAACTTAc                                                                                                                 <  1:681010‑M2/36‑1 (MQ=255)
                                        tccgacggcggcacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCGTCTGAGTATTGTGGCGACCGAAGGCAACTTAc                                                                                                                 >  2:403826‑M2/109‑144 (MQ=255)
                                                cgggcccgcgggcgctgaaaccgttgattgatgccgatctccttgacctcaatcagtggccggtgatcaacgccaccaccggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCgcggc                                                                                                    <  1:67874‑M2/49‑1 (MQ=255)
                                                  gcacagctggcgctgaaaccgttgaatgataccgatcttcttgacctcaatcagtggccggtgatccacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGcggcgg                                                                                                  >  1:481619‑M2/99‑149 (MQ=255)
                                                   cacagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCggcggc                                                                                                 >  1:927018‑M2/98‑149 (MQ=255)
                                                     cagctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatccgtggccggtgatcaacgctaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCgcggc                                                                                                    >  2:820076‑M2/96‑144 (MQ=255)
                                                      agctggcgctgaaaccgtggattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCgcggcg                                                                                                   <  2:793425‑M2/50‑1 (MQ=255)
                                                        ctggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCggcggc                                                                                                 >  2:212597‑M2/93‑144 (MQ=255)
                                                         tggcgctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGc                                                                                           >  1:232664‑M2/92‑149 (MQ=255)
                                                              ctgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGCAAGGGGCGGCGGCCCAAGc                                                                                           >  2:207386‑M2/87‑144 (MQ=255)
                                                               tgaaaccgttgattgatgccgatcttcttgacctcaatccgtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCg                                                                                          <  2:840553‑M2/59‑1 (MQ=255)
                                                               tgaaaccgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGAAAGTTTTTTTGTTGGCGCGGCGGCACAAGCGGTACa                                                                                     <  1:225307‑M2/64‑1 (MQ=255)
                                                               tgaaaccgttgatcgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACa                                                                                     <  1:268003‑M2/64‑1 (MQ=255)
                                                               cgaaaccgctgattgatgcctatcttctcgccctcaatcagtggccggtgaccaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGCACGGTCTCAACCGACTGAAAGGCGCGGCGGCACAAGCGGTACa                                                                                     <  1:224156‑M2/64‑1 (MQ=255)
                                                                   accgttgattgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTgc                                                                                 >  1:462053‑M2/82‑149 (MQ=255)
                                                                            tgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATAtt                                                                        >  1:925896‑M2/73‑149 (MQ=255)
                                                                            tgatgccgatcttcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATAtt                                                                        >  1:608869‑M2/73‑149 (MQ=255)
                                                                                        tcttgacctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCg                                                                 <  2:418872‑M2/84‑1 (MQ=255)
                                                                                           tgactcctatcagtggccggtgatcaaccccaccacgggcgtgggcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCt                                                              <  2:124483‑M2/87‑1 (MQ=255)
                                                                                              cctccatcaggggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATCATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATg                                                           <  2:113610‑M2/90‑1 (MQ=255)
                                                                                               ctcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGAACGAAGACAACTTACTGAAAGGCGCGGCGGCACCAGCGGTGCAGTGCGCCAATATGGCTTGCGGCTAtgg                                                          >  2:32895‑M2/54‑143 (MQ=255)
                                                                                                tcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAAc                                                    >  1:603677‑M2/53‑149 (MQ=255)
                                                                                                tcaatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGAGCGGCGGCACAACCGGTACAGCCGGCCAGTCCCCGTGTCGGCTatgcggggag                                                    >  1:398019‑M2/53‑145 (MQ=255)
                                                                                                ccagtcggggtccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACCGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCg                                                         <  2:537209‑M2/92‑1 (MQ=255)
                                                                                                 caatcagtggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCgg                                                        <  2:472433‑M2/93‑1 (MQ=255)
                                                                                                        tggccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACGTAGTGAAGGGCAGGACGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCCGtctc                                            >  1:445666‑M2/45‑149 (MQ=255)
                                                                                                           ccggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTa                                         >  1:537443‑M2/42‑149 (MQ=255)
                                                                                                            cggtgatcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAACATGCGGTTCGGCTATGCGGAAACTCAgtcg                                             >  2:472095‑M2/41‑143 (MQ=255)
                                                                                                                gatcaaggcgaccagcggggtgagcggtgcggggcgAGCGTCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTa                                         <  2:56506‑M2/108‑1 (MQ=255)
                                                                                                                 atcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGcggaaacgcaccactccgc                                        <  2:46886‑M2/109‑10 (MQ=255)
                                                                                                                  tcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAgg                                  >  1:855183‑M2/35‑149 (MQ=255)
                                                                                                                  tcaacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGAGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAgg                                  >  1:663489‑M2/35‑149 (MQ=255)
                                                                                                                    aacgccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGACAATACTCGGTTCGGCTATGCGGAAACGCAGCCtcctactga                                     >  2:506430‑M2/33‑140 (MQ=255)
                                                                                                                    aacgccaccagcggcgtgagcggtgaagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTGCGGCTCTGCGGAACCGCAGCCCCTTCTTTAAGGGt                                >  1:554164‑M2/33‑149 (MQ=255)
                                                                                                                       gccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACACGCGGTGCAGTGCGCCAATATTCGTTTCGGCTATGAGGAAAAGCAGTCTCTTATTTAAGGGGGCa                             >  1:13679‑M2/30‑149 (MQ=255)
                                                                                                                       gccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGgtgga                             >  1:273686‑M2/30‑147 (MQ=255)
                                                                                                                        ccaccagcggcgtgagcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCaa                            >  1:802408‑M2/29‑149 (MQ=255)
                                                                                                                                    tgagcggttcagggcgAGGATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCat                <  1:59077‑M2/133‑1 (MQ=255)
                                                                                                                                       gcggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATCTTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCattaa             <  1:872792‑M2/136‑1 (MQ=255)
                                                                                                                                        cggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATgaatacc                 >  2:368220‑M2/13‑141 (MQ=255)
                                                                                                                                         ggtgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCat                <  2:521307‑M2/133‑1 (MQ=255)
                                                                                                                                           tgcagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGCAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAAGTAt         >  1:767613‑M2/10‑149 (MQ=255)
                                                                                                                                             cagggcgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCa       >  1:529659‑M2/8‑149 (MQ=255)
                                                                                                                                                ggcgGGCATCTGATTATTGTGGCGACCGAAGACAATTTACAGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTAt         <  2:88714‑M2/140‑1 (MQ=255)
                                                                                                                                                  cgAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGTCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCCTTAATTATCAAACTg  >  1:627512‑M2/3‑149 (MQ=255)
                                                                                                                                                    aGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTCTCcaa     <  2:784714/144‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCGGGTGTGACCCAGGCGCAAGTCGCGCAAGTGTTACAGCAGGCGTATGCCCATAAACCGCTGGTGCGGCTGTATGACAAAGGCGTTCCGGCGCTGAAAAATGTCGTTGGGCTGCCATTTTGCGATATCGGGTTTGCCGTTCAGGGCGAGCATCTGATTATTGTGGCGACCGAAGACAACTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTG  >  CP009273/4145673‑4145967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.