Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 444,779 C→T W440* (TGG→TAG)  cyoB ← cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273444,7790CT97.1% 432.2 / NA 141W440* (TGG→TAG) cyoBcytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (69/67);  total (71/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

CAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGA  >  CP009273/444636‑444924
                                                                                                                                               |                                                                                                                                                 
taaTCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCTAACGCTTTAGGCTACCAg                                                                                                                                              <  2:1456971/148‑1 (MQ=255)
   tCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAg                                                                                                                                           >  2:1252519/1‑149 (MQ=255)
   tCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGAAAAAAGGCAACGACGACGACGATGCTCCAGACACAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGATTTAGGctacc                                                                                                                                                >  1:749601/1‑144 (MQ=255)
     tgctgGCTCAAACGACGGGTAATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAg                                                                                                                                              <  1:539737/144‑1 (MQ=255)
      gctgGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGta                                                                                                                                        >  2:1025886/1‑148 (MQ=255)
         ggCTCAAACTACGGGGCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATc                                                                                                                                     <  2:206192/149‑1 (MQ=255)
          gCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATcc                                                                                                                                    >  2:139981/1‑149 (MQ=255)
            tCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCt                                                                                                                                  <  2:877034/149‑1 (MQ=255)
            tCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCt                                                                                                                                  >  2:773232/1‑149 (MQ=255)
            tCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAATAGGTCATCCCt                                                                                                                                  >  2:843935/1‑149 (MQ=255)
             cAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCAt                                                                                                                                      >  1:241354/1‑144 (MQ=255)
              aacctccgGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATc                                                                                                                                     <  1:1351041/138‑1 (MQ=255)
              aaaCGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATc                                                                                                                                     >  1:678032/1‑144 (MQ=255)
               aaCGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCGAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGCCCACCAGTAggcccccc                                                                                                                                    >  1:799123/1‑138 (MQ=255)
                 cgacgGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTACGCTACCAGTAGGTCATCCCTGCGaa                                                                                                                             <  2:1115735/149‑1 (MQ=255)
                  gacgGGTCATGCCCATGACGCCCAGCGCATACAGTGGCATAAAGGCAACCAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTg                                                                                                                                 <  1:679251/144‑1 (MQ=255)
                  gacgGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAag                                                                                                                            >  2:1489348/1‑149 (MQ=255)
                    cgGGTCAGGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAagca                                                                                                                          <  2:34672/149‑1 (MQ=255)
                     gggTCATGCCCAGGACGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAagcagc                                                                                                                        <  2:598507/149‑1 (MQ=255)
                     gggTCAGGCCCAAGACGCCCCGCGCATACAGGGGCATAACGGAAACGAAGAACCCGATGATCCAGAACCAGAACGCGCGTTTACCCCTGGTTTCGTTCAGTTTGAAACCGAACTCTTTAGGCTACCAGTAGGTCATCCCTGCGAagcag                                                                                                                         <  2:551076/149‑1 (MQ=255)
                      ggTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGaa                                                                                                                             <  1:1122753/144‑1 (MQ=255)
                       gccATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGcc                                                                                                                       >  2:751252/3‑149 (MQ=255)
                          aTGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAagcag                                                                                                                         <  1:56936/144‑1 (MQ=255)
                          aTGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGaa                                                                                                                    >  2:1416127/1‑149 (MQ=255)
                          aTGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGaa                                                                                                                    <  2:1009000/149‑1 (MQ=255)
                            gCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGa                                                                                                                  >  2:1125940/1‑149 (MQ=255)
                              ccATGAAGCCCAGCGCATGCAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGGTTTAGGCTACCAGTAGGCCATGCCGGCGAAGCAGCCGa                                                                                                                     >  1:1505359/1‑144 (MQ=255)
                                aTGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCAc                                                                                                              >  2:1774670/1‑149 (MQ=255)
                                     gCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCAcgccgc                                                                                                         <  2:1256973/149‑1 (MQ=255)
                                      cccAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACgccgcc                                                                                                        >  2:384705/1‑149 (MQ=255)
                                      cccAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACgccgcc                                                                                                        >  2:1735596/1‑149 (MQ=255)
                                      cccAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCACCCGAAGACCAcg                                                                                                             >  1:1476783/1‑144 (MQ=255)
                                       ccAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCAcgc                                                                                                            >  1:1201663/1‑144 (MQ=255)
                                             cATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATGCCTGGGCAGCAGCCGAAGACCACGCCGCCga                                                                                                      >  1:414422/1‑144 (MQ=255)
                                                   gcggCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTa                                                                                           <  2:1905671/147‑1 (MQ=255)
                                                   gTGGCATAAAGGTAATGACGAAGACGATGACCCAGGATCAGATCGCGTCTTTACCCCAGGTTTAGTTTTGTTTTAAACCGAACCCACTAAGCGACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTa                                                                                           <  2:1948432/149‑1 (MQ=255)
                                                   gTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTa                                                                                           <  2:920361/149‑1 (MQ=255)
                                                     ggCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACg                                                                                              <  1:1271262/144‑1 (MQ=255)
                                                       cATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGtt                                                                                            <  1:516857/144‑1 (MQ=255)
                                                        aTAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGaa                                                                                      <  2:221471/149‑1 (MQ=255)
                                                         tAAAGGCAACGAAGAAGCCGATGATCCAGGACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTAt                                                                                          <  1:874484/144‑1 (MQ=255)
                                                         tAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGACCGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTAt                                                                                          <  1:888619/144‑1 (MQ=255)
                                                             ggCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTACGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGATCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGAACACGCAATGGaa                                                                                      >  1:498966/1‑144 (MQ=255)
                                                               cAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAGACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCa                                                                               >  2:1860387/1‑149 (MQ=255)
                                                               cAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGt                                                                                    >  1:1044892/1‑144 (MQ=255)
                                                                aaCGAAGAAGCCGATGATCCAGAACCAGACCGCGCGTTTACCCCAGGGTTCGTTCAGTTTGAAACCGAACGCTTTAGGATACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTg                                                                                   <  1:599671/144‑1 (MQ=255)
                                                                aaCGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCCCCGATGATCCCGGTATGGagggg                                                                                   >  1:699966/1‑140 (MQ=255)
                                                                 aCGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTgc                                                                                  <  1:1303696/144‑1 (MQ=255)
                                                                  cGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATc                                                                            <  2:1254271/149‑1 (MQ=255)
                                                                   gaaggaGCCGATGATGCGGAACCAGGACGCGCGTTTGCCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGGCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTgcgc                                                                                <  1:871293/144‑1 (MQ=255)
                                                                     agaagCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAgg                                                                         <  2:262519/149‑1 (MQ=255)
                                                                      gaagCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGCCCACGCCGCCGATGATCACGTTATGGAAGGGCGCAATCAGGa                                                                        >  2:910941/1‑149 (MQ=255)
                                                                           cGATGATCCAGAACCAGAACGCGCGATTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAgg                                                                   >  2:1187049/1‑149 (MQ=255)
                                                                             atgatCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCt                                                                 <  2:281057/149‑1 (MQ=255)
                                                                                atCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAgg                                                                   <  1:1683448/144‑1 (MQ=255)
                                                                                 tCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGc                                                                  <  1:684623/144‑1 (MQ=255)
                                                                                    agaaccagaacGCGCGTTTACCCCAGGTTGCGTTCAGTTTGAAACCGAACGCTATAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACGATGCCGCCGCTGATCACGTTAAGGAAGTGGGCACTCACGAACAGGCTGt                                                               >  1:993947/1‑144 (MQ=255)
                                                                                     gaaccataacGCGCGTTTACCTCATGTTTCTTTAAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGCCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGCTCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCa                                                         <  2:52224/149‑1 (MQ=255)
                                                                                     gaaccaggacGCGCGTGCACCCGAGGTTTCGGTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGtt                                                              <  1:1484425/144‑1 (MQ=255)
                                                                                      aaccagaccGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTCTAGGCTACCAGTAGGTCAGCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTa                                                             <  1:1407747/144‑1 (MQ=255)
                                                                                        ccagaacGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATg                                                           <  1:1314577/144‑1 (MQ=255)
                                                                                        ccagaacGCGCGTTTACCCAAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGaa                                                      <  2:775565/149‑1 (MQ=255)
                                                                                         cagaacGCGCGTTTACACCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGAAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGACACCGTTATGGAAGTGCGCAATCAGAAGAAGACTGTTagac                                                          >  1:108939/1‑141 (MQ=255)
                                                                                         cagaacGCGCGTCTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGc                                                          <  1:560782/144‑1 (MQ=255)
                                                                                            aacGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGaa                                                  <  2:1144243/149‑1 (MQ=255)
                                                                                               gcgcgTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGGTGATCACGTTATGGAAGTGCGCAATCAGGGACAGGCTGTTATGCAGAACg                                                    >  1:1158919/1‑144 (MQ=255)
                                                                                               gcgcgTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCAGTTAGGCAGAACg                                                    >  1:110699/1‑144 (MQ=255)
                                                                                                 gcgTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCcg                                             >  2:1521188/1‑149 (MQ=255)
                                                                                                 gcgTTTACCCCAGGGTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGaa                                                  <  1:850205/144‑1 (MQ=255)
                                                                                                 gcgTTGACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGTCGAAGACCACGCCGCCGATGATCACGTTATGGGAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGaa                                                  <  1:1503298/144‑1 (MQ=255)
                                                                                                    tttACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTCCCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTc                                               <  1:1609715/144‑1 (MQ=255)
                                                                                                     ttACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGcc                                         <  2:697412/149‑1 (MQ=255)
                                                                                                         cccAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACACGCAGTTATGCAGAACGAAGTCCgcgc                                          >  1:243044/1‑144 (MQ=255)
                                                                                                          ccAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGcc                                         <  1:616247/144‑1 (MQ=255)
                                                                                                            ctaaagcGTTCGGTTTCAAACTGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGGTATGCAGAACGAAGTCCGCGCCCGGTACg                                  >  2:1583716/7‑149 (MQ=255)
                                                                                                             ggTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATTACGTTATGGAAGTGCGCAATCAGGCCCAGGCTGTTCTGCAGAACGCAGCCCGCGCCCgg                                      <  1:1346785/144‑1 (MQ=255)
                                                                                                                   gTTCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagca                           <  2:1179229/149‑1 (MQ=255)
                                                                                                                    ttCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagcag                          <  2:477277/149‑1 (MQ=255)
                                                                                                                    ttCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGACCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATACAGGACGAAGTCCGCGCCCGGTCGGGcc                               >  1:1155372/1‑144 (MQ=255)
                                                                                                                    ttCAGTTTGAAACCGAACGCTTTAGACTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTCCGGcc                               >  1:401819/1‑144 (MQ=255)
                                                                                                                     tCAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTGATGCAGGACGAAGTCCGCGCCCGGTGCGGCca                              >  1:48529/1‑144 (MQ=255)
                                                                                                                      cAGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGCCTGTTATGCAGAACGAAGTCCGCCCACGGTACGGccgg                             >  1:126283/1‑142 (MQ=255)
                                                                                                                       aGTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGAGCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCccgc                            >  1:636818/1‑144 (MQ=255)
                                                                                                                        gtgtGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGACGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGGACGGCcagcg                           >  1:1693489/4‑143 (MQ=255)
                                                                                                                        gTTTGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagca                           <  1:1084913/144‑1 (MQ=255)
                                                                                                                         tttGAAACCGAACGCTTTAGGCTACCCGTAGGTCATCCATGCGAAGCAGCCGAAGACCACGCCGCCGATGATCAAGTTATGGAAGGGCGCAATCGGAAACAGGCGGTTATGCGGAACGAAATAGTGGCGCGGTAAGGCccgcag                          >  1:379480/1‑144 (MQ=255)
                                                                                                                         tttGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAGCGAAGTCCGCGCCCGGTCCGCGCAGCGGCCCGc                     >  2:60923/1‑149 (MQ=255)
                                                                                                                         tttGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGc                     <  2:711364/149‑1 (MQ=255)
                                                                                                                         tttGAAACCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGc                     >  2:102371/1‑149 (MQ=255)
                                                                                                                              aaCCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGATATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCGAGTc                >  2:731231/1‑149 (MQ=255)
                                                                                                                               aCCGAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCa               >  2:1487581/1‑149 (MQ=255)
                                                                                                                               aCCGAACGCTTCAGCCTACCAGGAGGTCATCCCTCCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCa               <  2:589170/149‑1 (MQ=255)
                                                                                                                                  gAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGCACGAGATCCGCGCCCGGTACGGGCAGACGCACGCAAGTAATcc            >  2:49737/1‑149 (MQ=255)
                                                                                                                                  gAACGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATcc            >  2:1378166/1‑149 (MQ=255)
                                                                                                                                   aaCGCTTTAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATccc           <  2:1309809/149‑1 (MQ=255)
                                                                                                                                      gccttAGGGTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGcc        <  2:498029/146‑1 (MQ=255)
                                                                                                                                        tttAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCa      >  2:1651836/1‑149 (MQ=255)
                                                                                                                                          tAGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCAcc    >  2:1549157/1‑149 (MQ=255)
                                                                                                                                           aGGCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGCAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCAAGCAGCACGCCAGTCATCCCGCCCACCg   >  2:1555163/1‑149 (MQ=255)
                                                                                                                                            ggCTACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCga  >  2:673127/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGA  >  CP009273/444636‑444924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: