Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,440,869 Δ1 bp 5.3% coding (672/906 nt) feaR ← transcriptional activator for tynA and feaB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,440,8690A. 5.3% 191.9 / 6.7 75coding (672/906 nt)feaRtranscriptional activator for tynA and feaB
Reads supporting (aligned to +/- strand):  ref base A (39/29);  new base . (2/2);  total (43/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-01

AATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCACGACGAGGTTGAACAGATTCCCGCTGATGAAGTACCGGGCGCAGCAGACACACCATC  >  CP009273/1440724‑1441016
                                                                                                                                                   |                                                                                                                                                   
aaTGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTCCTCACAGAccg                                                                                                                                                  >  1:1146144/1‑148 (MQ=255)
aaTGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAAAATTCGGTAAAAACTACGTACTGACATACCt                                                                                                                                                  <  1:647625/149‑1 (MQ=255)
aaTCAAATCTGATGCCTTCCAGTTTTTCATCATCTGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACTGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CCGCAAACATTCGGTACAAACTACGTACTGACATACCt                                                                                                                                                  <  1:609636/149‑1 (MQ=255)
 aTGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCACAACTTTAA‑‑CGGCAAACATTCGGTACAAACTACGTACTCACATACCTg                                                                                                                                                 >  2:660149/1‑149 (MQ=255)
   gAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGtc                                                                                                                                               <  1:1161976/149‑1 (MQ=255)
     aaGCCGATGCCTGCCAGTTTTTCATCATCTGCGGCATGGCGCATCGCATCTGCACAAAAATCGAGACGACGGTTCCGAATATATTGCGCGCCTACCACACCTTTAT‑‑CGGCAAACATTCGGGACAAACTACGTACTGATGTACCTGtctc                                                                                                                                             >  1:520120/1‑149 (MQ=255)
     aaGCCGATGCCTGCCAGTTTTTCATCATCTGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGGACTGACATACCTGtctc                                                                                                                                             >  1:418994/1‑149 (MQ=255)
     aaGCCGATGCCTGCCAGTTTTTCATCATCTGCGGCATGGCGAATCGCATCTGCACAAAAAGCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTAATGACATACCTGtctc                                                                                                                                             >  2:1127387/1‑149 (MQ=255)
             gcctccaatttttgCACCATTCGCGGCATGCCGAGTCGCATCTGCACAAAAACCGAGCCGACGGTTACGAATATATTGCGCTACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTa                                                                                                                                     <  2:332938/140‑1 (MQ=255)
               ctgccAGTTTTTCATCATCTGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATc                                                                                                                                   <  2:664136/149‑1 (MQ=255)
                tgtcAGTTTTTCATCATCTGAGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCGTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATcc                                                                                                                                  <  1:236980/146‑1 (MQ=255)
                      tttttCATCATCTGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCg                                                                                                                            >  1:1145021/1‑149 (MQ=255)
                        tttCATCATCTGCGGCATGGCGAATAGCATCTGCACAAAAATCGAGACGACGGTTACGACTGTATTACGCGACTACCAAACCTTTAT‑‑CGGAAAACATTCGGTACAAACTACGTACTTACCTACCTGTATCCCCGGCTATGCAATCCggg                                                                                                                          >  1:742333/1‑149 (MQ=255)
                           catcatCTGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCCGGCGt                                                                                                                       >  1:661416/1‑149 (MQ=255)
                           catcatCTGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCGCTCCGGCTATCCACCCCGGGCGt                                                                                                                       >  2:319995/1‑149 (MQ=255)
                             tcatcTGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTaa                                                                                                                     >  1:1217730/1‑149 (MQ=255)
                                  tGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCCCGCCGGGCGTAGTAtct                                                                                                                >  1:661376/1‑149 (MQ=255)
                                  tGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATAtct                                                                                                                >  2:807295/1‑149 (MQ=255)
                                    cGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAACCTACGTACTGCCATCCCTGTCTCTCCGGCTATCCACTCCGGGCGTCATATctct                                                                                                              >  2:477400/1‑149 (MQ=255)
                                    cGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATctct                                                                                                              <  1:319508/149‑1 (MQ=255)
                                      gCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTc                                                                                                            >  1:885031/1‑149 (MQ=255)
                                        aTGGCGAATCGCATCGGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTcgc                                                                                                          <  1:80086/149‑1 (MQ=255)
                                         tGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTCCAAACTACGTACTGACATACCTGTCTCTCCGGCGATCCACACCGGGCGTAATATCTCTTcggg                                                                                                         >  2:170477/1‑147 (MQ=255)
                                          ggCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATACTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTCATAGCCCTTCGCGa                                                                                                        >  2:180416/1‑149 (MQ=255)
                                           gCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTcgcgga                                                                                                       >  1:757243/1‑147 (MQ=255)
                                           gCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGaa                                                                                                       <  2:30466/149‑1 (MQ=255)
                                            cGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCCTACTATCCCTTGGCGAat                                                                                                      >  2:486995/1‑149 (MQ=255)
                                                    tcttGCACAAAAATGCGGCGGACGGTCACCAACATATTGCGCGACTACCAAGCCTTGAT‑‑CGGCAACCACTCGGTGCTAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTGCGCGAATATTATCGt                                                                                              <  2:109335/146‑1 (MQ=255)
                                                    aTCTGCACAAAAATCGAGACGACGGTTACGAATAGATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTGCGTACTGACATACCTGTCTCTCCGGCTATCCCCTCCGGGCGTAATATCTCTTCGCGAATATTATCGt                                                                                              <  2:1169135/149‑1 (MQ=255)
                                                      cTGCACAAAAATCGAGACGACGGTTACGAATATTTTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCt                                                                                            >  1:796659/1‑149 (MQ=255)
                                                      cTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCCCTTCGCGAATATTATTGTCt                                                                                            >  1:707007/1‑149 (MQ=255)
                                                       tGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTCCAAACTACGTACTGACATCCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTAt                                                                                          >  2:1153536/1‑149 (MQ=255)
                                                       tGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATCCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTAt                                                                                          >  2:786278/1‑149 (MQ=255)
                                                          acaAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAACCCTTTAT‑‑CGGCAAACAATCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTGTCCACTCCGGGCGGAATATCTCTTCGCGGATATTATCCTCAATcg                                                                                        >  1:700576/1‑148 (MQ=255)
                                                          acaAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCa                                                                                        <  1:767192/149‑1 (MQ=255)
                                                          acaAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCa                                                                                        >  1:949558/1‑149 (MQ=255)
                                                          acaAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCa                                                                                        <  1:307026/149‑1 (MQ=255)
                                                          acaAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCCCTTCGCGAATATTATCGTCTCTCa                                                                                        >  1:462322/1‑149 (MQ=255)
                                                          acaAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTCTAT‑‑CGGCAAACATTAGGAACCAACAACGTACTGCCATACCCGTCTCTCCGGCTATCCACTCCGGGCGTGATATCACTTCGCCCATATTTTCGTCCATCa                                                                                        >  2:687902/1‑149 (MQ=255)
                                                            aaaaaTCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTCCGCGAATATTATCGTCTATCAAc                                                                                      >  1:275586/1‑149 (MQ=255)
                                                             acaaTCGAGCCGACGGTTACGAATACATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACg                                                                                     <  1:357670/147‑1 (MQ=255)
                                                                 tCGAGACGACGGGTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAAc                                                                                 <  1:7607/149‑1 (MQ=255)
                                                                 gcGAGACGACGGTTACGAATATACTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACAGTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAAc                                                                                 <  2:95715/148‑1 (MQ=255)
                                                                       cgacgGTTACGAATATATGGCGCGACTACCAAACCTTTATGCCAGCAAAATCGAAAAGAAATTTTACCTAACATCCCTGTCTCTCCGGCTATCCACTCCGGGCGTAGTATCTCTTGGCGAATTTTATCGTCTATCACCGTAACCACtgc                                                                           >  1:909350/1‑147 (MQ=255)
                                                                        gacgGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACtttt                                                                          >  2:733054/1‑149 (MQ=255)
                                                                             ttACGAATATATTGCGCGACTACCAACCCTTTAC‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGaaa                                                                     <  2:98119/149‑1 (MQ=255)
                                                                                 gAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCAACTCCGGGCGTAATATCCCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTGTAAACtta                                                                 >  2:14029/1‑147 (MQ=255)
                                                                                  aaTATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACACCTTTTTGAAACTGAc                                                                >  2:1180682/1‑149 (MQ=255)
                                                                                          gcgcGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCCCTTTTTGAAACTGACGTTCacga                                                        >  2:774450/1‑149 (MQ=255)
                                                                                           cgcgACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCacgac                                                       <  1:807295/149‑1 (MQ=255)
                                                                                            gcgACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCCCACGGCGAAAATTATCGTCTAGCAACGTAACCACTTTTTGAAACCGGCGATAacgacg                                                      >  2:603222/1‑149 (MQ=255)
                                                                                             ccaCTACAAACCTTTTAC‑‑CGGCAAACATTCGGTACAAACTACGTACTGCCATCCCTGTCTCTCCGGCTATCCTCTCCGGGCGTAATACCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCAcgacga                                                     <  2:175797/147‑1 (MQ=255)
                                                                                              gACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGCATATTATCGTCTTTCAACGTAACCATTTTTTGATAGTGACGTTCACGACGAg                                                    >  1:998063/1‑149 (MQ=255)
                                                                                                    cAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCACGACGAGGTTGaa                                              >  2:1144950/1‑149 (MQ=255)
                                                                                                       aCCTTTAT‑‑CGGCAAACATTCGGTACAAACCACGTACTGACATACCTGCCTCTCCGGCTAGCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCACGACGAGGTTGAACAg                                           <  1:235878/149‑1 (MQ=255)
                                                                                                        aactgtaatttgCAAAAATTCTTTACAAATGACGTACTGCGATACCTGTCTCTTCGGCTATCCACTTCGGGCGTAATATATCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCACGACGAGGTTGAACa                                            <  1:1181734/138‑1 (MQ=255)
                                                                                                         cTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTAACGCCGAGGTTGAACAGAt                                         >  2:543267/1‑149 (MQ=255)
                                                                                                         cTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTCAACGACGGGGTTGAACAGAt                                         >  2:121450/1‑149 (MQ=255)
                                                                                                              t‑‑CGGCAAACATTCGGTACTAACTACCTACTGCCATACCTTGCTCTCCGGCTATCCACTTCGGTCGTAATATCGCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTCACGTTCACGACGAGGTTGAACAGATTCCCg                                    <  2:854257/149‑1 (MQ=255)
                                                                                                                   gCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACACCGGGCGTAATACCTCTTCGCGAATACTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCACGACGAGGTTGAACACACTccccccg                                 >  2:1189782/1‑145 (MQ=255)
                                                                                                                   gCAAACATGCGGTACAAACGACGGACTGACATACCTGTCTCTCCGGCTGTCCACGCCGGGCGGAATATCTCTTCGCGAAGATTATCGTCTATCAACGTAACCACTTTTTGACACTGACGTTCACGACGGGGATGAACAGATTCCCGccg                                 >  2:1088958/1‑147 (MQ=255)
                                                                                                                     aaaCATTCGGTACAAACTACGTACTGACATACCTGGCTCTCCGGCTATACACTCCGGGCGTAATATCTCTTCGCGCATATTATCTTCTAGCAACGTAACCACTTTTTAAAATTGACTTTCACGACCAGGGAGAACCGATTcccagagat                               >  2:857024/1‑143 (MQ=255)
                                                                                                                        cATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTAACGACGAGGTTGAACAGCTTCCCGCTGATGaa                            >  2:524742/1‑149 (MQ=255)
                                                                                                                         aTTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCACGACGAGGTTGAACAGATTCCCGCTGATGAAg                           <  1:436133/149‑1 (MQ=255)
                                                                                                                          ttCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAATCTGTCGTTCACGACGAGGTTGAACAGATTCCCGCtcaacacgt                          >  2:127683/1‑141 (MQ=255)
                                                                                                                              gTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCACGACGAGGTTGAACAGATTCCCGCTGATGAAGTACCg                      >  2:727098/1‑149 (MQ=255)
                                                                                                                                aCAAATCACGTACTTACCGACCTGTCTCTCAGGCTATCCACTCCGGGCGTAATATCTCTTCGCGCATATTATCGTCCATCAACGTCACCTCTTTTTGCGACTGACTTTCACGACGGGGTTGAACAGATTCCCGCTGATGAAGTACCggg                    <  1:186411/149‑1 (MQ=255)
                                                                                                                                aCAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCTAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCACGACGAGGTTGAACAGATTCCCGCTGATGAAGTACCggg                    <  2:900558/149‑1 (MQ=255)
                                                                                                                                aCAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCACGACGAGGTTGAACAGATTCCCGCTGATGAAGTACCggg                    <  1:160854/149‑1 (MQ=255)
                                                                                                                                   aaCTACGTACGTACATACCTGTCTCTCCGGCTATCCACTCCGTGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCACGACGAGGTTGAACAGATTCCCGCTGATGAAGTACCGGgcgc                 <  2:66634/149‑1 (MQ=255)
                                                                                                                                     cTACGTACTGACATCCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCACGACGAGGTTGAACAGATTCCCGCTGATGAAGTACCGGGCgcagc              <  1:1153536/149‑1 (MQ=255)
                                                                                                                                     cTACGTACTGACATCCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCACGACGAGGTTGAACAGATTCCCGCTGATGAAGTACCGGGCgcagc              <  1:786278/149‑1 (MQ=255)
                                                                                                                                                 aTACCTGTCTCTCCGGCTATCCCCTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGGCGTTCACGACGAGGTTGAACAGATTCCCGCTGATGAAGTACCGGGCGCAGCAGACACACCAt   <  1:548489/149‑1 (MQ=255)
                                                                                                                                                 aTACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCACGACGAGGTTGAACAGATTCCCGCTGATGAAGTACCGGGCGCAGCAGACACACCAt   <  2:1041696/149‑1 (MQ=255)
                                                                                                                                                  tACCTGTCGTTTCTGTTATCCCTTCCGGGCGTAATAGCTCTTGGCGAATAGTATCGTCTATCAACGTAACCACTTTTGGAAACTGACGTTCACGACGAGGTTGAACAGCTTCCCGCTGATGAAGTACCGGGCGCAGCAGACACACCATc  <  1:382931/149‑1 (MQ=255)
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AATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGCATGGCGAATCGCATCTGCACAAAAATCGAGACGACGGTTACGAATATATTGCGCGACTACCAAACCTTTAT‑‑CGGCAAACATTCGGTACAAACTACGTACTGACATACCTGTCTCTCCGGCTATCCACTCCGGGCGTAATATCTCTTCGCGAATATTATCGTCTATCAACGTAACCACTTTTTGAAACTGACGTTCACGACGAGGTTGAACAGATTCCCGCTGATGAAGTACCGGGCGCAGCAGACACACCATC  >  CP009273/1440724‑1441016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: