Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 777,280 C→T 6.1% intergenic (+172/‑261) lysQ → / → nadA tRNA‑Lys/quinolinate synthase, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273777,2800CT 6.1% 263.2 / 6.1 99intergenic (+172/‑261)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
Reads supporting (aligned to +/- strand):  ref base C (41/52);  new base T (3/3);  total (44/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

Only 100 of 103 total aligned reads displayed.

GCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGA  >  CP009273/777132‑777422
                                                                                                                                                    |                                                                                                                                              
gcTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGTGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGccc                                                                                                                                                <  1:39766/149‑1 (MQ=17)
gcTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAATAAGCGccc                                                                                                                                                >  1:642446/1‑149 (MQ=17)
gcTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGccc                                                                                                                                                <  1:932490/149‑1 (MQ=32)
  ttttttGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTa                                                                                                                                              >  1:943764/1‑149 (MQ=32)
   tttttGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaa                                                                                                                                             <  1:634999/149‑1 (MQ=32)
          tATCTGCGATACTCAAAGATTCGAACCTGCCGCAGGGTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCCCGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCT                                                                                                                                      >  2:692105‑M1/1‑149 (MQ=255)
          tATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGTGAGTAATCCTGCTCGCACCACCAATGTAAAAAAGCAACCTAAAAGCGCT                                                                                                                                      >  1:165751‑M1/1‑149 (MQ=255)
          tATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCCCGACCCACCAATGTAAACAAGCCCCCTAAAGGCGCT                                                                                                                                      >  1:45069‑M1/1‑149 (MQ=255)
          tATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCT                                                                                                                                      >  1:1114198‑M1/1‑149 (MQ=255)
           aTCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTT                                                                                                                                     >  2:207219‑M1/1‑149 (MQ=255)
            tCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTT                                                                                                                                    <  1:1217546‑M1/149‑1 (MQ=255)
            tCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGCAAAAAAGCGCCTTTAGGGCGCTTT                                                                                                                                    >  2:1063447‑M1/1‑149 (MQ=255)
              tGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTT                                                                                                                                  >  2:1005570‑M1/1‑149 (MQ=255)
              tGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTT                                                                                                                                  >  1:938576‑M1/1‑149 (MQ=255)
              tGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTT                                                                                                                                  >  2:194382‑M1/1‑149 (MQ=255)
              tGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTT                                                                                                                                  >  1:162782‑M1/1‑149 (MQ=255)
               gCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTT                                                                                                                                 <  2:32258‑M1/149‑1 (MQ=255)
               gCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCCTATTT                                                                                                                                 >  2:935768‑M1/1‑149 (MQ=255)
                      tCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGACATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGCCCCTTTTTTACTATTc                                                                                                                          >  2:383892/1‑149 (MQ=9)
                       cAAAGATTCGCACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGCCGCCGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCa                                                                                                                         <  1:289496/149‑1 (MQ=12)
                       cAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCa                                                                                                                         <  2:1023133/149‑1 (MQ=32)
                       cAAACATTCGCAGCTGCAGCAGGTTTTAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCa                                                                                                                         <  1:1090575/149‑1 (MQ=255)
                        aaaGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAg                                                                                                                        >  2:385073/1‑149 (MQ=32)
                             tttgtaCCCGCAGCAGGTTTGGGTTGAGCGCAGGGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATc                                                                                                                   <  2:314910/144‑1 (MQ=255)
                             ttCGAACCTGACGGAGGTTTGAGTTGAGCGCAGCCAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACTCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATc                                                                                                                   <  2:815303/149‑1 (MQ=11)
                             tcccaaCCTACAGCAATTTTCAGTCGAGCGCACCGAAACAACGGAGCCCCACGCGGCCACGGCCCAAAGAGCGATCGAAGCTAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCATGCATc                                                                                                                   <  2:521586/145‑1 (MQ=11)
                                gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTc                                                                                                                >  1:1209755/1‑149 (MQ=39)
                                gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCAGCCTc                                                                                                                >  1:628386/1‑149 (MQ=37)
                                     tGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAAttt                                                                                                           <  2:922917/149‑1 (MQ=255)
                                        cgcagGTTTGGGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCAc                                                                                                        <  1:249039/148‑1 (MQ=255)
                                        agcagTTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCCAAAGGCGCTTTTTTACTATTCAGGCATCCTCAATTTCAc                                                                                                        >  2:868637/1‑149 (MQ=255)
                                          cagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAGTTTCACtt                                                                                                      >  1:194346/1‑149 (MQ=255)
                                          cagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACtt                                                                                                      >  1:1117339/1‑149 (MQ=255)
                                            ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGCAAAAAAGCGCCTTTAGGGCGCTTTTTTacattggtgggtcgtgcaggatgactcgc                                                                                                    <  1:1063447‑M1/149‑30 (MQ=255)
                                               ttgagttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTaa                                                                                                 >  1:319575/1‑149 (MQ=255)
                                                 gggttgggCGCGGGGAAACAACGGAGCCGGTCAGGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCTCCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAc                                                                                               <  2:697290/147‑1 (MQ=17)
                                                 gggttgagCGCCGCGCAACAAGGGCGCCGCTCGCGGCGACGGCTCGAACGGCGAGCGACGCGTCTATGCCTGTATTACCCACCAATGTAAAAAAGCGCCCTAACCGCGCTTTCTTGCTATTCAGGCATCCTTAATTTCACtttttccac                                                                                               <  2:571627/147‑7 (MQ=9)
                                                 gagttgcgCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAc                                                                                               <  2:735217/149‑1 (MQ=255)
                                                 gagttgagCGCCGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGAGCGAGCGAAGCGACTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAc                                                                                               <  2:886736/149‑1 (MQ=255)
                                                 gagttgagCGCAGCGAAACAACGGCGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAc                                                                                               <  2:894774/149‑1 (MQ=255)
                                                  agttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCTTTTTTacattggtgggtcgtgcaggatgactcgcttcgct                                                                                              <  2:1103703‑M1/149‑36 (MQ=255)
                                                   gttgagCGCAGCGAAACAACGGAGCCGCTAGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCt                                                                                             >  1:556480/1‑149 (MQ=255)
                                                   ggtgagcgGAGCGAAGCAAGGGGGCCGGTCGCGGCGACGGCCCGATGGGCGGGGGAGGCGAGTCATCCTGCAGGACCCACGTATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTacattggtgggtcgtgcaggatgactcgcttcgctc                                                                                             <  2:1084301‑M1/147‑37 (MQ=255)
                                                    ttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGTGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtg                                                                                            >  1:550191/1‑149 (MQ=255)
                                                    ttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCCCGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTAACTTTGTAAACCtg                                                                                            >  1:582197/1‑149 (MQ=255)
                                                    ttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCCCTTTGTCAACCtc                                                                                            >  1:618843/1‑148 (MQ=255)
                                                    ttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtg                                                                                            >  1:489856/1‑149 (MQ=255)
                                                    ttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtg                                                                                            >  1:458886/1‑149 (MQ=255)
                                                     tgagCTCCGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGTGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGCAAAAACGCGCCTTTAGGGCGCTTTTTTacattggtgggtcgtgcaggatgactcgcttcgctcgc                                                                                           <  1:1121106‑M1/149‑39 (MQ=255)
                                                      gagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtgat                                                                                          <  2:331148/149‑1 (MQ=255)
                                                        gcgcAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtgctga                                                                                        >  1:103728/1‑149 (MQ=255)
                                                         cgcAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGAc                                                                                       >  1:1128919/1‑149 (MQ=255)
                                                          gcAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACa                                                                                      <  2:497805/149‑1 (MQ=255)
                                                                caacaccGGAGCCGCTCGTGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGCCa                                                                                <  2:1038066/148‑1 (MQ=255)
                                                                  acaacGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCaga                                                                              <  1:385073/149‑1 (MQ=255)
                                                                   caacGGTGCCGCTCGCGGCGGCGGACCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCagag                                                                             <  1:553121/149‑1 (MQ=255)
                                                                   caacGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCagag                                                                             <  2:336256/149‑1 (MQ=255)
                                                                       ggAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACCTTGTAAACCTGATGACATCGTCAGAGCTTa                                                                         <  1:650342/149‑1 (MQ=255)
                                                                       ggAGCCGCCCGCGGCGGCGCCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAACCGCCCTTTGTTTCTATTATGGGATCCTCAATTTCACTTTGTAAACCTTATGACATCGTCAGAGCTTa                                                                         <  1:601591/149‑1 (MQ=18)
                                                                        gtgCCGTTCGCGGCGCCGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTAc                                                                        <  2:173923/147‑1 (MQ=255)
                                                                        gAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTAc                                                                        >  1:983307/1‑149 (MQ=255)
                                                                         aGCCGCTCGCGGCGCCGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGAGGACATCGTCAGTGCTTACt                                                                       <  2:843255/149‑1 (MQ=255)
                                                                               tCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaa                                                                 <  2:642446/149‑1 (MQ=255)
                                                                                cgcgGCGACGGCCCGAAGGGCGAGCGAAGCTAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaag                                                                <  1:500528/149‑1 (MQ=255)
                                                                                  cggcgACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaagca                                                              >  1:1156430/1‑149 (MQ=255)
                                                                                     cgACGGCCCGAAGGGCGAGCTAAACTACTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAAct                                                           <  2:1178495/149‑1 (MQ=255)
                                                                                            ccGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTCAAGGCGCTTTTTTGCTATTCCGGAATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAAGCCTTACTGTGCAAGCAACTCTATGTc                                                    >  1:289881/1‑149 (MQ=25)
                                                                                             cGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCg                                                   <  2:372798/149‑1 (MQ=255)
                                                                                              gAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCgg                                                  <  1:655541/149‑1 (MQ=255)
                                                                                               aaGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGt                                                 <  2:279349/149‑1 (MQ=255)
                                                                                                aGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTg                                                <  2:559777/149‑1 (MQ=255)
                                                                                                   gcgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGaa                                             >  2:1154635/1‑149 (MQ=255)
                                                                                                   gcgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGaa                                             <  2:556480/149‑1 (MQ=255)
                                                                                                   gcgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGaa                                             <  1:328722/149‑1 (MQ=255)
                                                                                                   gcgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAAGCGCTTTTTTGATATTCAGGCATCCTCAATTTACCTTTGTAAACCTGATCAAATCGTCAAAGCTTCCTGTGACGGCAAATCTATGTCGGTGGaa                                             >  1:723852/1‑149 (MQ=21)
                                                                                                   gcgagcgaAGCGAGTCATCCGGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTCTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGaa                                             <  2:556511/149‑1 (MQ=255)
                                                                                                    cgagggaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAAt                                            <  2:1209811/149‑1 (MQ=255)
                                                                                                     gagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACACTCTGTCGCTGGAAtt                                           >  2:730613/1‑149 (MQ=255)
                                                                                                      agcgaagcgaGTCATCCTGCACGACCCACCCCTGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCTTCCCCAAGTTCACTTTGTAAACCTCATGAACTGGTCAGTGCTTACTGTGCAAGCAACTCTATGTCGATGGCAtga                                          >  2:619741/1‑147 (MQ=21)
                                                                                                        cgaagcgaGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCCTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAgg                                        <  2:619935/149‑1 (MQ=255)
                                                                                                                 tCATCCTGCACGACCCACCAATGTACCAAGGAAACTTTTAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCAATTTGTAAACCTGATGAAATCGTCAGAGCGTACTGTGCAACCAGCTCTCCGTCGGTGTGAGTAGGCGTAAAATg                               >  1:299396/1‑149 (MQ=255)
                                                                                                                 tCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAACGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTATACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTGGGCGTAAAATg                               <  2:147059/149‑1 (MQ=255)
                                                                                                                  cATCCTGCACGACCCGCCAATGTAATAAAGCGCCCTTAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGa                              <  1:748721/149‑1 (MQ=255)
                                                                                                                  cATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGa                              <  1:202515/149‑1 (MQ=255)
                                                                                                                   aTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGAc                             <  2:1209755/149‑1 (MQ=255)
                                                                                                                    tCCTGCACTACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACg                            <  1:1004232/149‑1 (MQ=255)
                                                                                                                     cgtGCACGACCCACCAGTAAAAAATGTCGCCCTAATGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGc                           <  2:1178117/147‑1 (MQ=255)
                                                                                                                      cTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCa                          >  2:209575/1‑149 (MQ=255)
                                                                                                                          ccGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACACAGTCAGAGCTTACTGTGCAAGCAACCCTATGTCCGTGGAATTAGGCGTAAAATGCCggacccc                      >  2:40366/2‑143 (MQ=38)
                                                                                                                          aCGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCt                      <  1:1085687/149‑1 (MQ=255)
                                                                                                                          aCGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGATTACCTTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCt                      <  1:659528/149‑1 (MQ=255)
                                                                                                                            gACCCACCAATGTAAAAAAACGACCTAAAGGCGCTGTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCCGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGc                    >  2:912603/1‑149 (MQ=255)
                                                                                                                                 accaATGTAAAAAAGCGCCCTAAAGGCGCGTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACAtt               <  2:491072/149‑1 (MQ=255)
                                                                                                                                 accaATGGAAAAAAGCGCCCTAAAGGCGCTTTTTTTCTAATCAGGCATCCTCAATTTCCCTTTGTAAACCTGAAGACATCGTCAGAGCTTACTGTTCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACAtt               <  1:21717/149‑1 (MQ=255)
                                                                                                                                     aTGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGc           <  1:271950/149‑1 (MQ=255)
                                                                                                                                        tAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCGTCCTGCACATTAGGCGTa        >  1:93544/1‑149 (MQ=255)
                                                                                                                                        tAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTa        >  1:93388/1‑149 (MQ=255)
                                                                                                                                          aaaaaGCGCCTTTAGGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAAt      >  2:814983/1‑149 (MQ=255)
                                                                                                                                            aaaGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTc    <  1:880263/149‑1 (MQ=255)
                                                                                                                                              aGCGCCCTAAAGGCGCTTTTTTacattggtgggtcgtgtaggatgactcgcttcgctcgcccttcgggccgtcgccgcgagcggctccgttgtttcgctgcgctcaactcaaacctgctgcaggttcgaatcttttagtatcgcagata  >  2:267227‑M1/1‑22 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGA  >  CP009273/777132‑777422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: