Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 916,181 G→C 5.5% E127Q (GAG→CAG)  macB → fused macrolide transporter subunits of ABC superfamily: ATP‑binding component/membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273916,1810GC 5.5% 222.4 / 3.9 80E127Q (GAG→CAG) macBfused macrolide transporter subunits of ABC superfamily: ATP‑binding component/membrane component
Reads supporting (aligned to +/- strand):  ref base G (28/47);  new base C (2/3);  total (30/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

CGCTGGCGCAACTGCGCCGCGAGCATTTCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGA  >  CP009273/916038‑916329
                                                                                                                                                |                                                                                                                                                    
tcgcggcgcagttGCGCCAGCGAGCATTTCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACGGCGACTGCTTCGTGCCCAGGAGtt                                                                                                                                                  >  2:720909/13‑149 (MQ=255)
      cgcAACTGCGCCGCGAGCATTTCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCaa                                                                                                                                           <  1:63852/149‑1 (MQ=255)
            tGCGCCGCGAGCATTTCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACggctg                                                                                                                                     >  2:978253/1‑149 (MQ=255)
            tGCGCCGCGAGCATTTCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACggctg                                                                                                                                     >  1:292258/1‑149 (MQ=255)
                 cgcgAGCATTTCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGCCCGGTTCGTGCCCAGGAGTTGCTGCAACggctggggct                                                                                                                                <  2:267280/149‑1 (MQ=255)
                 cgcgAGCATTTCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACggctggggct                                                                                                                                <  2:308422/149‑1 (MQ=255)
                 cgcgAGCATTTCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACggctggggct                                                                                                                                <  1:89091/149‑1 (MQ=255)
                 cgcgAGCATTTCCGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCTCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACggctggggct                                                                                                                                <  1:480608/149‑1 (MQ=255)
                    cgAGCATTTCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCAGTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACggctggggctgg                                                                                                                              <  2:459588/149‑1 (MQ=255)
                     gAGCATTTCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGCCGTTGCAGCAActcctgggcacgaa                                                                                                                             <  2:1214470/149‑14 (MQ=255)
                     gAGCATTTCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGCCGTTGCAGCAActcctgggcacgaa                                                                                                                             <  1:1110815/149‑14 (MQ=255)
                     gAGCATTTCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGCCGTTGCAGCAActcctgggcacgaa                                                                                                                             <  1:991966/149‑14 (MQ=255)
                      aGCATTTCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGaa                                                                                                                            <  2:16730/149‑1 (MQ=255)
                          tttCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGAcc                                                                                                                        <  1:411214/149‑1 (MQ=255)
                             cGGCTTTATTTTCCAGCGTTACCATTTGCGTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTa                                                                                                                     <  2:808462/149‑1 (MQ=255)
                              ggCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGACGACCGTAc                                                                                                                    >  2:150003/1‑149 (MQ=255)
                               gCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACa                                                                                                                   <  2:827825/149‑1 (MQ=255)
                                cTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACag                                                                                                                  <  2:340049/149‑1 (MQ=255)
                                 tttATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGGGTTGCTGCAACGGCTGGGGCTGGAAGACCGtcccga                                                                                                                 >  2:864355/1‑144 (MQ=255)
                                 tttATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGGACaga                                                                                                                 >  2:1003671/1‑149 (MQ=255)
                                 tttATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTAGGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACCGCTGGGGCTGGAAGACCGTCCagc                                                                                                                 >  2:143908/1‑148 (MQ=255)
                                 tttATTTTCCAGCGTTACCATTTGCTTTAAAATGCCATTTCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACaga                                                                                                                 >  1:299385/1‑149 (MQ=255)
                                       ttCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGtatta                                                                                                           <  2:792206/149‑1 (MQ=255)
                                         ccAGCGTTACCATTTGCTTTCGCATTTAACCGTCGAGCAGAACGTTGAAGGATCTGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCTTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATc                                                                                                         <  2:681725/149‑1 (MQ=255)
                                         ccAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATc                                                                                                         <  1:808806/149‑1 (MQ=255)
                                            gCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGTAGACCGTACAGAGTAATATCCgg                                                                                                      <  2:845789/149‑1 (MQ=255)
                                               ttACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGcac                                                                                                   >  2:358838/1‑149 (MQ=255)
                                                tACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGcaca                                                                                                  >  2:216723/1‑149 (MQ=255)
                                                     tttGCTTTCGCATTTACCCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCCGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCttt                                                                                             <  1:61398/149‑1 (MQ=255)
                                                     tttGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGGACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCttt                                                                                             <  1:199889/149‑1 (MQ=255)
                                                      ttGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTc                                                                                            <  2:1091490/149‑1 (MQ=255)
                                                       tGCTTTCGCATTTCACCGCCGAGCACAACCTTGAAGTACCCGTCGTCTATGCTGGTCTGGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCg                                                                                           <  2:659776/149‑1 (MQ=255)
                                                        gCTTTCGCATTTAACCGCCGGGCACAACGTTGAAGTACCCGCCGTCCATGCTGGGCTTGGGGGGAAACAGCGACTGCTTCGTGCCCAGGCGTTGCTGCGTCGGCGGGGGCTGGAATACCTGACAGATTATTTTCCGGCACAGCTTTCgg                                                                                          <  2:338329/149‑1 (MQ=255)
                                                          tttCGCATTTAACCGCCGAGTAGAACGTTGAAGTACCCTCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGggt                                                                                        <  1:1052885/149‑1 (MQ=255)
                                                           ttCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGggtg                                                                                       <  1:14978/149‑1 (MQ=255)
                                                            ccccctctAACCGCCGAGCAGAACGTTGGCGGCCCCGCCGTCTATGCTGGTCTTGAGCGGAAGCAGCGACTGCTTCGTGCCCAGGGGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGggtgg                                                                                      <  2:975705/142‑1 (MQ=255)
                                                             cGCAGTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGATTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGggtggt                                                                                     <  2:1087464/149‑1 (MQ=255)
                                                                aTTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGGGTTGCTGGCACGACTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGggtggacgg                                                                                  >  2:443025/1‑145 (MQ=255)
                                                                aTTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCGACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGAACAGCTATCGGGTGGTCAg                                                                                  >  1:411019/1‑149 (MQ=255)
                                                                    aaCCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAAc                                                                              <  2:292258/149‑1 (MQ=255)
                                                                       cgccgAGCAGAACGTTGAAGTACCCGCCGTCTATGCCGGCCTTGCGCGGAAACAGCGACTGCTTCGTGCCCAGCAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTCTTATCCGGCACAGCCTTCCGGTGGCCAGCAACacc                                                                           >  2:326792/1‑147 (MQ=255)
                                                                       cgccgAGCAGAACGTTGAAGTACCCGCCATCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGACCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTGTCCGGCACAGCTTTCGGGTGAGCAGCAACGgc                                                                           >  2:697362/1‑149 (MQ=255)
                                                                          cgAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCGTTCGGGTGGTCAGCAACAGcgcg                                                                        >  2:482696/1‑149 (MQ=255)
                                                                             gCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAGGACCGTACAGAGAATTATCCGGCAAGGCTTTCGGGGGGTCAGCAACAgcgactca                                                                     >  1:954781/1‑144 (MQ=255)
                                                                              cgcaaCGTTGAAGTCCGCGCCGTCTATGCTGGTCTTGGGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAg                                                                    <  2:827427/146‑1 (MQ=255)
                                                                              cAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAg                                                                    <  2:110604/149‑1 (MQ=255)
                                                                                   cGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGGAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGGCAGCAACAGCGCGCCAGCATcg                                                               >  1:120668/1‑149 (MQ=255)
                                                                                    gTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgc                                                              >  1:530382/1‑149 (MQ=255)
                                                                                     ttGAAGCACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACGGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcg                                                             <  2:185101/149‑1 (MQ=255)
                                                                                       gAAGTACCCGCCGTCTATGCTGCTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATcgcgcg                                                           <  2:324777/149‑1 (MQ=255)
                                                                                        gaGTACCCGCCGTCTATGCTGGTCTGGAGCGGAAACAGCGCCTGCTTCGTCCCCAGGCGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCTCAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCgg                                                          <  2:821491/148‑1 (MQ=255)
                                                                                            aCCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCGACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCAt                                                      >  1:352030/1‑149 (MQ=255)
                                                                                             cccGGCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCAtt                                                     <  2:1055922/149‑1 (MQ=255)
                                                                                              ccgccgTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATtg                                                    >  2:171893/1‑149 (MQ=255)
                                                                                                gccgTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATtgat                                                  >  2:196946/1‑149 (MQ=255)
                                                                                                     cgaTGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACg                                             <  1:1036154/147‑1 (MQ=255)
                                                                                                      tATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACgg                                            >  1:1066913/1‑149 (MQ=255)
                                                                                                        tGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACggtg                                          <  2:1010380/149‑1 (MQ=255)
                                                                                                        ggCTGGTCTTGAGCGGAAACAGGAACTGCTTCGGGCCCCGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCAGTGATGAACggtg                                          <  2:180862/148‑1 (MQ=255)
                                                                                                          cTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACggtggt                                        <  2:1045043/149‑1 (MQ=255)
                                                                                                             gTCTGGGGCGGAAACGGCGACTGTTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACGGCGCGTCAGCATCGCGCGGGCCTTGATGAACGGTGGTCAg                                     <  2:146999/149‑1 (MQ=255)
                                                                                                                       gAAACAGCGACTGCTTCGTGCCCAGGGGTTGCTGCAACGGCTGGGGCTGGAAGATCGTACAGAGTATTATCCGGCACAGCTTTCGGGGGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTg                           <  2:1061189/149‑1 (MQ=255)
                                                                                                                       gAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTg                           >  2:755622/1‑149 (MQ=255)
                                                                                                                       gAAACAGCGACTGCTTCGTGACCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTg                           <  1:864355/149‑1 (MQ=255)
                                                                                                                        aaaCAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGc                          >  1:160587/1‑149 (MQ=255)
                                                                                                                           cAGGGACCGCTTCGTGCCCAGGAGGTGCGGCGACTGCTGGAGCTGGAAGACCGTACAGAGTATTCTCCGGCACAGCTTTCTGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATGGATGAACGGTGGTCAGGTAATTCTTGCCGa                       <  1:533431/149‑1 (MQ=255)
                                                                                                                           cAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAGGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGa                       <  1:978253/149‑1 (MQ=255)
                                                                                                                           cAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGa                       <  1:324446/149‑1 (MQ=255)
                                                                                                                           cAGCGACTGCTTCGTGCCCAGCAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGGCAGGTAATTCTTGCCGa                       >  1:649191/1‑149 (MQ=255)
                                                                                                                            aGCGACTGCCTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGAt                      <  1:1134882/149‑1 (MQ=255)
                                                                                                                              cGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCGCAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGGCAGGTAATTCTTGCCGATGa                    >  2:978085/1‑149 (MQ=255)
                                                                                                                              cGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGa                    >  1:1139676/1‑149 (MQ=255)
                                                                                                                              cGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGa                    >  1:167179/1‑149 (MQ=255)
                                                                                                                               gACGGCCTCGGGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTGAttggtttaaataaa                   <  2:939657/149‑13 (MQ=255)
                                                                                                                                aCTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCCTTGATGAACGGTGGTCAGGTGATTCTTGCCGATGaac                  <  1:787585/149‑1 (MQ=255)
                                                                                                                                    cTTCGTGCCCAGGAGTTGCTGCACCGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCCGCATCGCGCGGGCATTGATGAACTGTGGTCAGGTAATTCTTGCCGATGaaccaac              <  2:727239/149‑1 (MQ=255)
                                                                                                                                    cTTCGTGCCCAGGAGTTGCTGCACCGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGaaccacc              <  2:829084/149‑3 (MQ=255)
                                                                                                                                            ccAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCAc      >  1:475144/1‑149 (MQ=255)
                                                                                                                                            ccAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCAc      >  2:591713/1‑149 (MQ=255)
                                                                                                                                              aGGAGTTGCTGGAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATGCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCACCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTg    <  1:915800/149‑1 (MQ=255)
                                                                                                                                                gAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGa  <  1:8870/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CGCTGGCGCAACTGCGCCGCGAGCATTTCGGCTTTATTTTCCAGCGTTACCATTTGCTTTCGCATTTAACCGCCGAGCAGAACGTTGAAGTACCCGCCGTCTATGCTGGTCTTGAGCGGAAACAGCGACTGCTTCGTGCCCAGGAGTTGCTGCAACGGCTGGGGCTGGAAGACCGTACAGAGTATTATCCGGCACAGCTTTCGGGTGGTCAGCAACAGCGCGTCAGCATCGCGCGGGCATTGATGAACGGTGGTCAGGTAATTCTTGCCGATGAACCAACCGGCGCACTGGA  >  CP009273/916038‑916329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: