| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 982,566 | C→A | 10.6% | intergenic (‑128/+475) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 982,566 | 0 | C | A | 10.6% | 106.5 / 3.5 | 44 | intergenic (‑128/+475) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (23/16); new base A (3/2); total (26/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACA > CP009273/982418‑982705 | cAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGGTTGGTGTCTTAATGTGTCTGCAGGCATCTTTCAATTc > 2:826400/1‑149 (MQ=255) atTTCGCTTAATCCTTCTTTATTACCCTCAGGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTa < 2:500794/149‑1 (MQ=255) gcTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTATCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGtt > 1:749444/1‑149 (MQ=255) tcgtcatTTTGTATTACCACCATGTTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCg < 2:43854/146‑1 (MQ=255) ccatcttgttttttctcATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGACTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCt < 2:832141/140‑1 (MQ=255) gTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGCGTGTTTATGTGGCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagat < 1:1095552/149‑1 (MQ=255) tttttttATGACACCTGCCAATGCCGTCAATAACACGAATCCCTAAAAATTTACGTAACTTTTAATGAGAGATTGGTGTCTTTCTGTGTCTGCAGGCATCTTTACATTAAAACTAACGTTTCGATACCGTGAAAGTGCTACcaagataa > 2:1134190/1‑149 (MQ=255) ttttttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTCTCGCTACCGTGAAAGTGCTACaaagataaa < 2:98605/149‑1 (MQ=255) aTGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTg < 1:825696/149‑1 (MQ=255) aTGACACCTGCCAATGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGTGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTTCCGTGAAAGTGCTACAACAATAAAAATTTg > 2:759960/1‑149 (MQ=255) gaactccggcACGGCTGTCAATGAGGTCTGGCAATAAAAATTTACGGAACCATTGATGAGAGTTTGGTGTTTTTATGTGTCTTCAGGCATTTTTCCATTCAAACCAAGGTTTCGCTACCGTGAAAGTGCTATAAAGATAAAGATTTGGt < 2:325296/140‑1 (MQ=255) ccTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCa > 2:937033/1‑149 (MQ=255) cGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaaaa > 1:713887/1‑149 (MQ=255) gTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTATGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTAGCTACGGTGCAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAat > 2:133954/1‑149 (MQ=255) tCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTTGTTTCAAAAAGAAAAAata > 2:227498/1‑149 (MQ=255) cAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAatat > 1:1232238/1‑149 (MQ=255) aaGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTACTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTaa > 2:1232067/1‑149 (MQ=255) cTGGCAATAAAAATTTACGTATCTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCCAACTAACGTTGTGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCaaa < 1:826400/149‑1 (MQ=255) tGTCAATAAAAATTTACGGAACTATTGATGAGTGTTTGGTGTCGTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTTAAAGTTCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCaaaa < 2:184021/149‑1 (MQ=255) aaaaaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAAt > 1:965971/1‑149 (MQ=255) aaaaaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAAt > 1:928122/1‑149 (MQ=255) aaaaaTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATAGGTAACCAAAAGTAAAAt > 1:934135/1‑149 (MQ=255) aTTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTaa < 1:53952/149‑1 (MQ=255) tttACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAg > 1:1147823/1‑149 (MQ=255) tttACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATGTAAg > 2:246418/1‑149 (MQ=255) acagAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGaa < 1:618286/146‑1 (MQ=255) tATTGATAAGAGTTTGGTGTCTTTATGTTTCAGGAGGCATCCTACCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGAGAAAAAAAAAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgtg < 2:809032/149‑1 (MQ=255) atgaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAacac > 2:647634/1‑149 (MQ=255) agagTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGt > 1:941751/1‑149 (MQ=255) agTTTGGTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCa > 2:1101169/1‑149 (MQ=255) tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCCAACAGAAAAAAGATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCata > 1:1158681/1‑149 (MQ=255) tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCata > 1:248575/1‑149 (MQ=255) ggTGTCTTTATGTGTCTGCAGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATAttt > 1:1170635/1‑149 (MQ=255) gtgcccttATGTGTCTGGAGGGGACGGTCCACTCAATCTACCGTTTCCCGACCGTGAAAGTGCTAAAGAGATAAAGATTTGGAAAAAAAAAAAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTc < 2:335631/143‑1 (MQ=255) gtCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACAGTCATATTTCCa > 1:966114/1‑149 (MQ=255) gtCTTTATGTGTCAGCAGGCATCTTTCCATTAAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTAAAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCa < 2:1227370/149‑1 (MQ=255) tCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGCTTTGGTTTCAAAAAGAAAAAAGATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCAt > 1:664963/1‑149 (MQ=255) tttATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATag > 1:267640/1‑149 (MQ=255) tCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAGGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtga > 1:1024124/1‑149 (MQ=255) attGCAGGCAGCTTTCCAGTCAAAGTAACGTGTCGGTGCCGTGAAAGTGATAAAAAGATAAAGATTTAAATAAAAAAAAAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtga < 2:444280/147‑1 (MQ=255) gCAGGCCTCTTTCCATTCAAACTAACGTTTCGCCACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGCAAAAATATTTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGtgatga < 1:292509/149‑1 (MQ=255) caagCATCTTTCCATTCAAACTAACGTTTCACAACCGTGATAGTGCTACAAAGATAAAGATTCGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTgatgat < 1:63744/146‑1 (MQ=255) aGGCATCTTACCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGAACTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGata > 2:450010/1‑149 (MQ=255) tCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTAc > 1:1018091/1‑149 (MQ=255) cTTTCCATTCAAACTAACGTTTCGCTACCGTGAAATTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATACAGACGTGATGATATTTACa < 1:898689/149‑1 (MQ=255) | CAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACA > CP009273/982418‑982705 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |