Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,702,919 C→G 7.4% Q582E (CAA→GAA)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,702,9190CG 7.4% 217.0 / 8.4 88Q582E (CAA→GAA) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base C (43/38);  new base G (2/5);  total (45/43)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

AGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCC  >  CP009273/1702771‑1703065
                                                                                                                                                     |                                                                                                                                                   
aGCGGAAGCCAGAGGCAAACAGGCGGGAATGCAGCAAACTAACGACGCAGCAACCGCTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGaac                                                                                                                                                     <  1:833911/149‑1 (MQ=37)
aGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGaac                                                                                                                                                     >  1:203496/1‑149 (MQ=255)
aGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGaac                                                                                                                                                     >  1:819587/1‑149 (MQ=255)
   gcAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGaacaac                                                                                                                                                  <  1:124607/149‑1 (MQ=255)
    cacgCCAGAGCGAAACAGGCAGAACCGCAGCAAACTAACGACGCAGCACCCGTTGCTGATCCACGTAAACCTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAacaaca                                                                                                                                                 <  1:1065589/146‑1 (MQ=255)
     aaGCCAGCGCGAAACAGGCAGAAGTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGaacaaccg                                                                                                                                                >  2:846311/1‑147 (MQ=255)
      aGCCAGACCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCCGCTATCGCCCGCGCCAAAGCGCGGAAGCGGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAgg                                                                                                                                               <  2:381074/149‑1 (MQ=255)
         cAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCg                                                                                                                                            >  1:564335/1‑149 (MQ=255)
         cAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCg                                                                                                                                            >  1:300530/1‑149 (MQ=255)
           gggCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGAt                                                                                                                                          <  2:25844/147‑1 (MQ=255)
            agCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATc                                                                                                                                         >  2:718985/1‑149 (MQ=255)
                  aCAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGGACCgcgcg                                                                                                                                   >  1:865962/1‑148 (MQ=255)
                    aGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCaaa                                                                                                                                 <  1:321014/149‑1 (MQ=255)
                       cAGAACGGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCATCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCGACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAgct                                                                                                                              <  2:925773/149‑2 (MQ=21)
                         gAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGATCGATCCCCGCAAAgcggc                                                                                                                            >  2:383662/1‑149 (MQ=255)
                           aCTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGGAAACAGCGACTCCCGGGCCGCATCAGTCAAGCTGGAACAGGAACAGGCCAAAGCGCAACCAGAACAACAGGACGATCCGCGCAAAGccgccg                                                                                                                          >  1:293716/1‑149 (MQ=255)
                            cTGCAGCAACCTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGt                                                                                                                         <  1:886300/149‑1 (MQ=255)
                             tGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTc                                                                                                                        <  2:1094803/149‑1 (MQ=255)
                             tGCACCAAACCAACGACGCAGCAACCTTTGCTGATCCACGGAAAACGGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAACCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTc                                                                                                                        <  2:121296/149‑1 (MQ=18)
                              gcagcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCg                                                                                                                       >  1:832542/1‑149 (MQ=255)
                              gcagcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCg                                                                                                                       >  1:1108606/1‑149 (MQ=255)
                                 gccaacgacggacgCAGCAACCGGGGCCGATCCACGCAAAGCCGCCGTTGAAGCAGCTATCGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                    <  2:819587/139‑1 (MQ=11)
                                     aCTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGGCGAAGCCGc                                                                                                                >  2:136885/1‑149 (MQ=255)
                                          cgacgCAGCCACCGTGGCTGAGCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCATAGCCGCCGTCGAAGCCGCTATTg                                                                                                           <  1:680001/149‑1 (MQ=255)
                                          cgacgCAGCAACCCTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTg                                                                                                           <  1:280805/149‑1 (MQ=255)
                                           gacgCAGCAACCGTTGCTGATCCACGTAAAACTGCAGGCGTAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCCATTGc                                                                                                          >  1:605757/1‑149 (MQ=18)
                                              gcagcaACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGTGACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCg                                                                                                      >  1:1212373/1‑149 (MQ=255)
                                              gcagcaACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCg                                                                                                      <  1:951421/149‑1 (MQ=255)
                                              gcagcaACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCg                                                                                                      >  1:1121576/1‑149 (MQ=255)
                                                    aCCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGACCCGCGCAGACACGCCGTCGCAACCGCTATTGCCCGCGCCag                                                                                                >  2:286644/1‑148 (MQ=18)
                                                      cgcgtttccgcgctgTAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGACCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAg                                                                                              <  2:1065469/135‑1 (MQ=1)
                                                       gTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGGCGTCGAAGCCGCTATTGCCCGTGCCAAAgc                                                                                             >  1:284421/1‑149 (MQ=255)
                                                       gTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgc                                                                                             >  1:1001982/1‑149 (MQ=255)
                                                              aTCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAg                                                                                      >  1:793101/1‑149 (MQ=255)
                                                              aTCCACGTAAAACCGCCGTGGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAg                                                                                      <  1:308069/149‑1 (MQ=255)
                                                                 cACGTAAAACTGCCGTTGAAGCGGCTAGCGCCCGCGCCACAGCGCGCAAGCTGGCACAGCAACAGGCTAATGCGGAACCAGGACACCGGGTCGTTCCGCGCATAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCagcgctgtctggggg                                                                                   <  2:324273/149‑15 (MQ=17)
                                                                   cGTAAGACCGCCGTTGAAGAGGCTATCGCCCGCGCCAAAGCGCGCAAGCCGGACCAGCAACAGGCTCATGCGGAACCAGAACAACAGGTCGATCCGCGCAACGCCGCCGTCGAAGCCGCTACTGCCCGTGCCAAAGCGCGCAAGCTGGa                                                                                 <  2:336841/149‑1 (MQ=14)
                                                                   cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGa                                                                                 <  1:421143/149‑1 (MQ=255)
                                                                   cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGa                                                                                 <  1:421044/149‑1 (MQ=255)
                                                                   cGTAAAACTGCCGGTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGa                                                                                 <  2:246477/149‑1 (MQ=255)
                                                                    gTACAACTGCCGTGGAAGCAGCTACCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGaa                                                                                <  2:834345/149‑1 (MQ=255)
                                                                     tAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCGAGCTGGGAc                                                                               >  1:1065469/1‑149 (MQ=255)
                                                                      aaaaCTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAACGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACa                                                                              <  2:922473/149‑1 (MQ=255)
                                                                           tgacGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAAc                                                                         <  2:1164119/146‑1 (MQ=255)
                                                                           tGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCATGCTGGAAGAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCGAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAGCCGCGCAAGCTGGAACAGCaaa                                                                         >  2:926681/1‑148 (MQ=14)
                                                                           ggCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAAc                                                                         <  2:293716/148‑1 (MQ=255)
                                                                             ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAg                                                                       >  1:86155/1‑149 (MQ=255)
                                                                               gTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGGACAGCAACAGGCTAATGCGGAACCAGAACAACAGCACCATCCGCGCTAAACGGCCGACGCAGCCGCTATTACACGACCCAAAGCACGCAAGCTGGAACAGCAAccgcc                                                                     >  2:4155/1‑145 (MQ=14)
                                                                               gTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGGGCCAGAACAACAGGACGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGACCGTGCCAAAGAACGCAAGCGACAACAGCAACAGGc                                                                     >  2:346507/1‑149 (MQ=14)
                                                                               gTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATGGCCCGGGCCAAAGCACGCAAGCGGGAACAGCAACAGGc                                                                     >  2:775693/1‑149 (MQ=255)
                                                                                ttGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTg                                                                                                           <  2:993849/111‑1 (MQ=255)
                                                                                ttGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTg                                                                                                           >  1:993849/1‑111 (MQ=255)
                                                                                   gAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGTACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTaa                                                                  <  1:301210/149‑1 (MQ=255)
                                                                                    aaGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGt                                                                                                                         >  1:509807/1‑94 (MQ=255)
                                                                                    aaGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGt                                                                                                                         <  2:509807/94‑1 (MQ=255)
                                                                                     agcagcTATTGACCGCGCCAAAGCGCGCAAGCTGGATCAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATg                                                                <  2:549526/149‑1 (MQ=255)
                                                                                     agcagcTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCACGCTGGAACAGCAACAGGCTAATg                                                                <  1:679880/149‑1 (MQ=255)
                                                                                     agcagcCAGCGCCCGCGCCAACGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATg                                                                <  2:700140/149‑1 (MQ=255)
                                                                                      gcagcTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAGGCGCGCAGGCTGGAACAGCAACAGGCTAATGc                                                               >  1:10955/1‑149 (MQ=255)
                                                                                      gcagcTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCCGGAACAGCAACAGGCTAATGc                                                               >  1:679129/1‑149 (MQ=255)
                                                                                      gcagcTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGc                                                               >  2:1015263/1‑149 (MQ=255)
                                                                                      gcagcTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGCCGAAGCCGCTATTGCACGGGCCAAAGCGCGCAAGCTGAAACAGCAACAGGCGAATGc                                                               >  2:288931/1‑149 (MQ=14)
                                                                                        agcTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGTAGCATCACGCCGAGCCAACGCGCGCAAACAGCAACAGCAACAGGCTAATGCgg                                                             >  1:897530/1‑149 (MQ=14)
                                                                                            aTCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCGGGAACAGCAACAGGCTAATGCGGAAcg                                                         >  1:141593/1‑148 (MQ=255)
                                                                                             tCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCaaa                                                                                               <  2:201273/110‑1 (MQ=38)
                                                                                             tCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCaaa                                                                                               >  1:201273/1‑110 (MQ=40)
                                                                                              cGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCag                                                       <  2:793101/149‑1 (MQ=12)
                                                                                                     cacgaaGCGCCCAAGCTGGCACAGCAACAGGCAAATGCGGAACCAGAACCACCGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACCGCAATAGGCTATTGCGGAACCagaattaca                                                <  2:728016/145‑6 (MQ=14)
                                                                                                      ccAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAg                                               >  1:44262/1‑149 (MQ=255)
                                                                                                          aGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCg                                           <  1:718985/149‑1 (MQ=255)
                                                                                                                 aaGCTGGACCAGCAACAGGCTAATGCGTACCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCcgcg                                    <  1:706315/149‑1 (MQ=255)
                                                                                                                  aGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCgcgc                                   <  1:200908/149‑1 (MQ=255)
                                                                                                                    cTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAAGAGGTCGATCCGCGaaa                                 >  1:142656/1‑149 (MQ=255)
                                                                                                                    cTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCaa                                 >  1:697169/1‑149 (MQ=255)
                                                                                                                    cTGGAACAGCAACAGGCTAATCCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCaa                                 >  1:879023/1‑149 (MQ=255)
                                                                                                                        aaCAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGCACCAGACGCACTGGTCGATCAGAGCAAAgcc                             >  1:281327/1‑149 (MQ=14)
                                                                                                                        aaCAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCGGAAGAAAAGGCGGATCCGCGCGAAggc                             >  2:292004/1‑147 (MQ=14)
                                                                                                                             ccaCAGCCAAAGGCGGAACCAGAACCCCAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATCGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGt                        <  2:194247/147‑1 (MQ=14)
                                                                                                                             cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACCGGCTACTGCGGAGCCAGAAAGCCGGGGGCATCCGCGCAAAGCAGCAGt                        >  2:930520/1‑149 (MQ=11)
                                                                                                                              aaCAGGCTAATGCGGACCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTc                       <  1:528918/149‑1 (MQ=255)
                                                                                                                              aaCAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTc                       <  1:782357/149‑1 (MQ=255)
                                                                                                                                  ggcaaccgCGGACCCAGCACCACTGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                   <  2:203496/142‑1 (MQ=14)
                                                                                                                                    cTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCGGCCGTCGCAGcc                 >  1:319898/1‑149 (MQ=255)
                                                                                                                                    cTAATGCGGAACCAGAACAACAGGTCGATCCGAACCAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGcc                 >  1:1225243/1‑149 (MQ=255)
                                                                                                                                     tAATGCGGAACCAGAACCACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCg                <  1:490571/149‑1 (MQ=255)
                                                                                                                                     tAATGCGGAACCAGAACCACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCg                <  1:1189888/149‑1 (MQ=255)
                                                                                                                                     tAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGa                                                            >  1:21505/1‑105 (MQ=40)
                                                                                                                                     gaaTGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACCGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCg                <  1:352624/148‑1 (MQ=255)
                                                                                                                                         gCGGAAGCAGAACACCAGGCCGACCCCCGGAAAGCCGCCGTCGAAGCCGCGATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAt            <  2:648646/149‑1 (MQ=14)
                                                                                                                                         gCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACCGGCTAACGCGGTTTTACTAGAGCAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCtgt            >  1:711142/1‑147 (MQ=14)
                                                                                                                                            gAACCAGCACAACGGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGc         <  1:1127598/149‑1 (MQ=255)
                                                                                                                                             aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGcc        >  1:1145217/1‑149 (MQ=255)
                                                                                                                                             aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCGGCTATTGca        >  1:574020/1‑148 (MQ=255)
                                                                                                                                               gcacaacaacTGGTCGACCCGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGAAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCg      <  1:460944/145‑1 (MQ=14)
                                                                                                                                                 aGAACAACAGGTCGATCCGCGCAAAGCCACCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTg    >  1:1189963/1‑149 (MQ=255)
                                                                                                                                                   aacaaaAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCCACCGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGcc  >  2:1135439/1‑149 (MQ=11)
                                                                                                                                                     |                                                                                                                                                   
AGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCC  >  CP009273/1702771‑1703065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: