Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	344,799	A→T	7.1%	E136V (GAG→GTG)	prpB →	2‑methylisocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	344,799	0	A	T	7.1%	165.9 / 2.9	68	E136V (GAG→GTG)	prpB	2‑methylisocitrate lyase
Reads supporting (aligned to +/- strand):  ref base A (31/30);  new base T (2/3);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

GATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTG  >  CP009273/344651‑344947                                                                                                                                                       |                                                                                                                                                     gATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAagaaga                                                                                                                                                      <  1:766644/149‑1 (MQ=255)                  ttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGGTCGTCTCGAAAGAAGAGATGGTGGATCGGAGcc                                                                                                                                     >  2:950390/1‑149 (MQ=255)                       ccTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCcgcgcg                                                                                                                                >  1:773665/1‑149 (MQ=255)                         tttAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCgcggc                                                                                                                              >  1:435697/1‑149 (MQ=255)                          ttAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGGAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGTGATGGTGGATCGGATCCGCgcggcg                                                                                                                             >  1:978075/1‑149 (MQ=255)                          ttAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGTGATGGTGGATCGGATCCGCgcggcg                                                                                                                             >  1:978143/1‑149 (MQ=255)                             aCGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTg                                                                                                                          >  1:141568/1‑149 (MQ=255)                              cGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGGGCGGGAGGATTGCCGATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTgg                                                                                                                         <  2:318811/149‑1 (MQ=255)                                   cgcgcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGCAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑GT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCg                                                                                                                    >  1:1099047/1‑149 (MQ=255)                                   cgcgcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGC‑AT‑ACAGCGCTCGTCTCGCACGCAGAGATGGTGGATCGGCTCCGCGCGGCGCTCGCTGCg                                                                                                                    >  1:334662/1‑149 (MQ=255)                                    gcgcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGa                                                                                                                   >  2:1096186/1‑149 (MQ=255)                                       cACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTg                                                                                                                          <  2:1062033/139‑1 (MQ=255)                                       cACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTg                                                                                                                          >  1:1062033/1‑139 (MQ=255)                                        aCCGTGAAATCGAGGACTAAAGCCGGTGCGGCAGGATTGCATGCTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAAc                                                                                                               <  1:30290/149‑1 (MQ=255)                                        aCCGTGAAACCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAAc                                                                                                               <  1:1025304/149‑1 (MQ=255)                                         ccGTGAAATCGATGATTACAGCCGGTGCGGCAGGATTGCATATTGACGATCAGGTTCGTGCGCAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGCGATGGTGGATCGGATCCGCGCGGCGAGGGATGCGAAGAcc                                                                                                              >  2:797052/1‑149 (MQ=255)                                         ccGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGCGAAcc                                                                                                              >  1:728501/1‑149 (MQ=255)                                          cGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGCTGCGAAAACCg                                                                                                             >  1:134871/1‑149 (MQ=255)                                            tGAACTCGACGGTTCAAGCCGGTGCGGCAGGAATGCATGTTGAAGATCAGGGTGTTGCGAAACGCGGCTGTCAGCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGGGATGGTGGATCGGATCCGCGCGGCGGTGGATGGGAAAACCGAt                                                                                                           >  2:422012/1‑149 (MQ=255)                                                  cgttgatTAAAGCCGGTGCGGCAGGATTGCATCTTGAAGAGTGGGTTGGGTCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCTATCGTCTCGAAAGAAGAGATGGTGGATCGGTTCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAt                                                                                                     <  2:991729/146‑1 (MQ=255)                                                   gatgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtt                                                                                                    >  2:982772/1‑149 (MQ=255)                                                   gatgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTAATTGGCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGGGGATGCGAAAACCGAGcccgctt                                                                                                    >  1:236995/1‑144 (MQ=255)                                                      ggttAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGACAGCAGAGCTGCTGGTTCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtg                                                                                                 <  2:933559/147‑1 (MQ=255)                                                      gatTAAGGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCGGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtg                                                                                                 <  2:157941/149‑1 (MQ=255)                                                      gatTAAAGCCGGTGCGGGAGGATTGCATATTGAAGATCAGGTTGGTGCGAAGCGCTGCGGTCATCGTCCGA‑AT‑AGTGCGATCGTCTCGAAAGAAGAGATGGTGGTTCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtg                                                                                                 <  2:157898/149‑1 (MQ=255)                                                       atTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGTGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtgt                                                                                                <  2:978143/149‑1 (MQ=255)                                                       atTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGTGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtgt                                                                                                <  2:978075/149‑1 (MQ=255)                                                        ttAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAGCGCTGCTGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtgtg                                                                                               <  1:235455/149‑1 (MQ=255)                                                                     ggCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGCTCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCa                                                                                  <  1:660826/149‑1 (MQ=255)                                                                      gCAGGATTGCATATTGAAGGTCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCAc                                                                                 <  2:79763/149‑1 (MQ=255)                                                                      gCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCAc                                                                                 >  2:492057/1‑149 (MQ=255)                                                                       cAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGcccc                                                                                >  2:461030/1‑149 (MQ=255)                                                                        aGGATTGCATTTTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCg                                                                               <  1:644972/149‑1 (MQ=255)                                                                          gATTGCATATTGAAGTTCAGGTTGGTGCGAAACGCGGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGAt                                                                             <  1:147700/149‑1 (MQ=255)                                                                          gATTGCATATTGAAGACCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGAt                                                                             <  1:365486/149‑1 (MQ=255)                                                                           aTTGCCTATGGAGGACCAGGTTGGTGCGAAACGCGGCGGTCCTCGTACGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCAGCGATg                                                                            <  2:586341/149‑1 (MQ=255)                                                                             tgactcgtgaacgacAGGTTGGTGCGCTACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGGCTCGCAAGAAGAGACGGTGGATCGGAGCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGct                                                                          <  2:863696/135‑1 (MQ=255)                                                                             tGCATATTGGAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑TAAGCGGTCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGct                                                                          <  2:504533/149‑1 (MQ=255)                                                                                 tatTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGCTGCGCAAGCCGATCCTGCTTTTGTGATCATGGGGCGGACCGATGCTCTgg                                                                      >  1:711276/1‑149 (MQ=255)                                                                                  atTGAATATCAGGGTGGGGCGAAACGTTGTGTTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGc                                                                     <  2:235250/149‑1 (MQ=255)                                                                                      aaGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTa                                                                 <  1:978841/149‑1 (MQ=255)                                                                                        ggtCAGGTGGGGGCGAAACGCTGCGGTCATCGTCCGA‑AT‑CAAGGCCTCGGCTCGACAGATGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGAGCCTGAGTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTaga                                                               <  2:1056908/147‑1 (MQ=255)                                                                                        gaccAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTCGATCGGATCCGCGCGGCGGTGGATGCGCAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGGGGTgga                                                               >  1:1110303/4‑149 (MQ=255)                                                                                        gATCAGGTTGGTGCGAAACGCTGAAGACA‑‑GTCAGTTATGGAAGCGATCGTCTCGCCAGAAGAGATGGTGGATAGGATCCGCGCGGCGGTGGAAGGGAAGACCGATCATGGTGTTGTGAACCGGGAGCGCACCCATGCTCTCGCggcgga                                                               >  2:576384/1‑145 (MQ=255)                                                                                               ttggcggggaatgttGCGGTCTGCGTCGGA‑GT‑AAAGCGGTCTGCTCGTAAGCAGAGATGTTGGGTCGGCTCCGCGCGGCGGTGGACGGGTCACCCGATCCTGATTTTGGGATCATGGCGGGCACCGATGCTCTGGCGGTAGAGGGGCTg                                                        <  2:437700/135‑1 (MQ=255)                                                                                               ttGGTGCGAAACGCTGCGGTCATCGTCCGA‑CT‑AAAGCGATCGTCACGAAAGAAGAGATGGTGGATCGGAAGCGCGCGGCGGTGGAGGCGAAAACCGAGCCTGCACATGTGGTGATGGCGGGCGCCGATGCCCTGGCGGTGGAGAGGCTg                                                        >  2:1106973/1‑149 (MQ=255)                                                                                                 gtttcGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGGAGAGGGGCTGGa                                                      <  2:236995/145‑1 (MQ=255)                                                                                                 ggTGCGAAACGCTGCGGTCATCGTCCCA‑AC‑AAAGCGATCGCCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGa                                                      <  1:1061790/149‑1 (MQ=255)                                                                                                 ggTGCGAAACGCCGCGGTCATCGTCCGA‑AT‑AAACAGATCGTCTCGAAAGAAGTGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATCTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGa                                                      <  2:690716/149‑1 (MQ=255)                                                                                                      gAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGAGGCGAAAACCGATCCTGATGTTGTGATCGTGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATgcag                                                 >  2:706799/1‑147 (MQ=255)                                                                                                          cGCTGCGGTCATCGCCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGAt                                             <  2:771549/149‑1 (MQ=255)                                                                                                           gCTGCCGTCATCGGCCGA‑AT‑AAAGCGATCGTCGCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATc                                            <  2:263973/149‑1 (MQ=255)                                                                                                               cGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGc                                        >  2:465355/1‑149 (MQ=255)                                                                                                                  tCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTg                                     <  1:982772/149‑1 (MQ=255)                                                                                                                    aTCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTgcg                                   >  1:1187528/1‑149 (MQ=255)                                                                                                                    aTCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTgcg                                   >  1:1187557/1‑149 (MQ=255)                                                                                                                     tCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTgcgc                                  >  1:220109/1‑149 (MQ=255)                                                                                                                          cGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGcc                             <  1:1111975/149‑1 (MQ=255)                                                                                                                          cGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATGTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGACGGGCTGGATGCGGCGAGCGAGCGTGCGCAGGcc                             >  2:938889/1‑149 (MQ=255)                                                                                                                                 aaaGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGCACGACGGTGCGCAGGccgaggt                        >  2:15229/1‑144 (MQ=255)                                                                                                                                 aaaGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGt                        >  1:439970/1‑149 (MQ=255)                                                                                                                                 aaaGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTACGt                        >  2:290539/1‑149 (MQ=255)                                                                                                                                   aGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCCGGCCTATGTTg                      >  1:737089/1‑149 (MQ=255)                                                                                                                                   aGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTCTGTTg                      >  1:222301/1‑149 (MQ=255)                                                                                                                                      gATCGTCTCGAAAGAAGAGATGGGGGCTCGGCTCCGCGCGGCGGTGTATGCGAACACCGCTCCTGATCTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAg                   <  2:141568/149‑1 (MQ=255)                                                                                                                                         cGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCgg                <  2:237857/149‑1 (MQ=255)                                                                                                                                         cGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGGGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCgg                <  2:134871/149‑1 (MQ=255)                                                                                                                                               gAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCGCCGATGCTCTGGCGGTAGAGGGGCTGGATGGGGCGATAGAGCGTGCGCCGGCCTATGTTTGAGAGGGTGcgg          >  2:833554/1‑147 (MQ=255)                                                                                                                                               gAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCg          >  2:1150949/1‑149 (MQ=255)                                                                                                                                                  agaagaGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGCTCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGAGCTGGATGCGGCGATCGAGCGGGCGCAGACCTATGTTGAAGCGGGTGCCgcga       >  1:413061/1‑149 (MQ=255)                                                                                                                                                       agaTGGTGGATCGGATCCGCGCGGCGGTGGATGCGACAACCGATCCTGATATTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGCGGGGCGGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGACGCGGGTGCCGAGCtgttg  >  2:46756/1‑149 (MQ=255)                                                                                                                                                       |                                                                                                                                                     GATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGA‑AT‑AAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTG  >  CP009273/344651‑344947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑