Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,831,581	A→T	7.6%	intergenic (‑117/+32)	hydN ← / ← ascG	formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,831,581	0	A	T	7.6%	209.4 / 6.7	75	intergenic (‑117/+32)	hydN/ascG	formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit/asc operon transcriptional repressor; prpBC operon repressor
Reads supporting (aligned to +/- strand):  ref base A (37/32);  new base T (2/4);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01

CTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGA  >  CP009273/2831436‑2831727                                                                                                                                                  |                                                                                                                                                   ccctcgTCAGCAATGATGAAACGGTGCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGacac                                                                                                                                                 <  1:994770/145‑1 (MQ=255)   cgggTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGTTACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTa                                                                                                                                               <  2:75385/146‑1 (MQ=255)      gTCAGCAATGGTGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAAGCATGCCAGTTTTTAATTTACTGTTATTTAAGGGAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTg                                                                                                                                            <  1:1166712/149‑1 (MQ=255)       ttaGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGa                                                                                                                                           <  1:990074/147‑1 (MQ=255)       tCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTgc                                                                                                                                           >  2:1189935/1‑148 (MQ=255)          gCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGGTTTTAATTTCCTGTTATTTAAGGGAATTAATTTCTGTAATGCAGGATAAACGATGTCATCGACACGAGTGACgc                                                                                                                                        >  1:495211/1‑148 (MQ=255)           cAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGAt                                                                                                                                       <  2:1172851/149‑1 (MQ=255)           cAATGAGGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGAt                                                                                                                                       <  2:934187/149‑1 (MQ=255)              tgatgaAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGAc                                                                                                                                    <  2:1075852/149‑1 (MQ=255)                 tgaAACGGTTCATCAAATTCGCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGTCACTAGTGTCGATGACAtc                                                                                                                                 <  2:349831/149‑2 (MQ=255)                     aCGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAATGACGATGACATGtgat                                                                                                                             >  1:127918/1‑149 (MQ=255)                      cGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACAGTCACAAATCATGCCAGTTTTTAATTTAATGTTATTTAAGGAAATAAATTTCTGTAAGGCAGGCAAATCGATGTACTCGACACCAGGGCCGATGGCATGtgatg                                                                                                                            >  2:68549/1‑149 (MQ=255)                         ttCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACa                                                                                                                         >  1:116791/1‑149 (MQ=255)                            atcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATg                                                                                                                      >  2:1132871/1‑149 (MQ=255)                             tcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGCCGATGACATGTGATGACAATGt                                                                                                                     >  2:454635/1‑149 (MQ=255)                             tcaAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTGAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACGAGTGACGATGACATGTGATGACAATGt                                                                                                                     >  1:862769/1‑149 (MQ=255)                               aaaTTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGAGGACATGTGATGACAATGttt                                                                                                                   >  2:1208659/1‑149 (MQ=255)                               aaaTTCTCCAGCAATGACAGTTAATACGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGttt                                                                                                                   >  1:620881/1‑149 (MQ=255)                                     tcCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTCCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGAGATGACAATGTTTATcgcg                                                                                                             >  2:710276/1‑149 (MQ=255)                                     tcCAGCAATGACAATTAATGCGCCGATACATTCCCAAATCATGCCAGTTTTTAATTTACTGTTATTTCCGGAAATTAATTTCTGTAATGCAGGAAAAACGACGTCATCGACACTAGTGACGATGCCAGTGGATGACAAGGtttcgagcg                                                                                                             >  2:124183/1‑143 (MQ=255)                                       cAGCAATGACAGTTAATGCGCCGATACATTCACAAATCCTGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAACACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGaa                                                                                                           <  1:311504/149‑1 (MQ=255)                                       cAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGaa                                                                                                           <  1:311456/149‑1 (MQ=255)                                       cAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGAAGACAATGTTTATCGCGaa                                                                                                           >  1:1082983/1‑149 (MQ=255)                                           aaTGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGAAATGTGATGACAATGTTTATCGCGAAGGAg                                                                                                       <  2:629959/149‑1 (MQ=255)                                            aTGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGc                                                                                                      >  1:528333/1‑149 (MQ=255)                                             tGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGGTGACATGTGATGACAATGTTTATCGCGAAGGAGCa                                                                                                     >  1:984400/1‑149 (MQ=255)                                             tGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGaaga                                                                                                     >  2:398509/1‑146 (MQ=255)                                             tGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACAAGTGATGACAACGATTATCGCGAAGGAGCa                                                                                                     >  2:1020635/1‑149 (MQ=255)                                              gACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGACATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCaa                                                                                                    <  2:896677/149‑1 (MQ=255)                                                 aGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtga                                                                                                 <  1:1020849/149‑1 (MQ=255)                                                 aGTTAATGCGCCGATACATGCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGATAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtga                                                                                                 <  1:1159435/149‑1 (MQ=255)                                                  gTTAATCGGACGATACATTCACAAGTCATGCCAGTTTTGAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAAtgag                                                                                                <  2:259845/149‑1 (MQ=255)                                                        gcgcCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTGAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTc                                                                                          >  2:625804/1‑149 (MQ=255)                                                                  aTTCACAAATCATGCCAGTTTTTAATTTATTTTTATTTAAGAAAATTAATTGCTGTAATGCAGGAAAAACGTTATCCTCGACACTATTGACGTTGCCATTTAATGACAATGTTTATCGCGATGGCCCAAggagggatcgccggaggatc                                                                                >  2:264087/1‑136 (MQ=255)                                                                   ttCACAAATCCTGCCAGTTTTCAATTTACTGTTATTTAAGGAAATTAACTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTCTACCGCGAAGGAGCAATGAGTGCGTCGCGGCGGATCa                                                                               <  1:655592/149‑1 (MQ=255)                                                                    tCACAAATCATGCCAGTTTTTAATTTACGGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAg                                                                              >  1:744202/1‑149 (MQ=255)                                                                     cacaAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGt                                                                             >  1:931072/1‑149 (MQ=255)                                                                      acaAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGtt                                                                            >  1:1080023/1‑149 (MQ=255)                                                                        aacttgcggcgatgtttAATGAACTGTTATTTAAGGAAATTAATTTCTGTAGTGCAGGAAAAACGATATCATCGACACTAGTGACGATGACATGTGATGACAATGTTTAACGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGtttt                                                                          >  2:939891/15‑149 (MQ=255)                                                                            cATGCCTGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGc                                                                      <  2:1088040/149‑1 (MQ=255)                                                                            cATGCCATTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGGAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGc                                                                      <  2:88651/149‑1 (MQ=255)                                                                               gCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGAAAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGa                                                                   <  2:1128834/149‑1 (MQ=255)                                                                                 cAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTACCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGagg                                                                 >  1:174234/1‑147 (MQ=255)                                                                                 cAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGGAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGTGGCGGATCAGTTTTCTGCTGAAg                                                                 <  2:843174/149‑1 (MQ=255)                                                                                 cAGTTTTGAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAACGAGCCACGATTGAGGCGCGGCGGATCAGTTTTCCGCTGAAg                                                                 <  1:299132/149‑1 (MQ=255)                                                                                       ttAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACGGTGTGTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTc                                                           >  1:1215378/1‑149 (MQ=255)                                                                                        tAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcg                                                          >  2:1137083/1‑149 (MQ=255)                                                                                           tttaacgTTATTTAAGTAAATTAAGTTCTTTAATGCAGGAAACACGATGTGATCCACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGTGCAATGAGTTAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcggcg                                                       <  1:54800/143‑1 (MQ=255)                                                                                            ttACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTcggcgg                                                      <  2:238928/149‑1 (MQ=255)                                                                                             tACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGt                                                     <  2:1004431/149‑1 (MQ=255)                                                                                              aCTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTg                                                    >  2:398028/1‑149 (MQ=255)                                                                                              aCTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGAGCAGTTTTCCGCTGAAGGTTTTCGGCGGTg                                                    >  1:402264/1‑149 (MQ=255)                                                                                                   tATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGATACTAGTGAGGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGaaa                                               <  1:793689/149‑1 (MQ=255)                                                                                                    aTTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAAt                                              >  1:1161775/1‑149 (MQ=255)                                                                                                          ggAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGc                                        >  2:735591/1‑149 (MQ=255)                                                                                                           gAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAACGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGcc                                       <  2:993683/149‑1 (MQ=255)                                                                                                            aaatatattTCTGCAATGCAGGAAAAACGATGGCACCGTCACTAGTGACGATGACATGTGATGACAATGTGTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCa                                      <  2:392321/143‑1 (MQ=255)                                                                                                             aattaattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCTCTGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCAt                                     >  1:286745/1‑149 (MQ=255)                                                                                                             aattaattTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCAt                                     >  1:410090/1‑149 (MQ=255)                                                                                                                   tttCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCa                               <  2:1138297/149‑1 (MQ=255)                                                                                                                    ttCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCCAGCAt                              >  1:1053458/1‑149 (MQ=255)                                                                                                                    gtCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGAAAAGGGTGAGACGCGGCGGATCCGGTTTCCGCTGAAGGTGTTCGGCGGTGAGAAATCCCCGCCATCGAGcgt                              >  2:462734/2‑147 (MQ=255)                                                                                                                        ctAATGCCGGAAAAACGATGCCAGTCACACTAGTGACGATGACATGTGCTGACAATGTTTATCGCGAAGGAGCAATGAGTGAGGCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGTTGAGAAAGCCCCGCCATCGAGCATaaaa                          <  2:1196432/148‑1 (MQ=255)                                                                                                                         tAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCGATGAGTGAGTCGCGGCGGGTCAGTTTTCCGCTGAAGGCTTTCGGCGGTGAGGAATCCACGCCAGCGAGCATaaaaa                         >  1:536820/1‑149 (MQ=255)                                                                                                                            tGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGGGAGAAATCCCCGCCATCGAGCATAAAAATCa                      >  2:127144/1‑149 (MQ=255)                                                                                                                                  aaaaaCGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTc                <  1:1132871/149‑1 (MQ=255)                                                                                                                                       cGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCaata           <  2:1201917/149‑1 (MQ=255)                                                                                                                                         gtttCATCGACACTAGTGATGATGACTTGTGATGATAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCaataat         <  1:463189/146‑1 (MQ=255)                                                                                                                                         aTGTCACCGACACTAGGGACGATGACATGGGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCaataat         <  2:557220/149‑1 (MQ=255)                                                                                                                                            tCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGGTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTc      >  1:51946/1‑149 (MQ=255)                                                                                                                                              aTCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCt    >  2:56366/1‑149 (MQ=255)                                                                                                                                              aTCGTCACTAGTGTCGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCt    >  2:1235131/1‑149 (MQ=255)                                                                                                                                              aTCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCt    <  1:1116763/149‑1 (MQ=255)                                                                                                                                               tCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTg   <  1:397566/149‑1 (MQ=255)                                                                                                                                               tCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTg   <  2:195098/149‑1 (MQ=255)                                                                                                                                                cGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAACGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGa  <  1:527506/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   CTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAATCAGCCGTCCAATAATTTCCTGA  >  CP009273/2831436‑2831727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑