Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,911,379 A→C 8.0% intergenic (+207/+25) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/(D)‑glucarate dehydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,911,3790AC 8.0% 219.9 / 8.8 85intergenic (+207/+25)barA/gudDhybrid sensory histidine kinase, in two‑component regulatory system with UvrY/(D)‑glucarate dehydratase 1
Reads supporting (aligned to +/- strand):  ref base A (35/42);  new base C (2/5);  total (38/47)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

AAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCAC  >  CP009273/2911231‑2911526
                                                                                                                                                    |                                                                                                                                                   
aaGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGa                                                                                                                                                     >  1:514149/1‑149 (MQ=255)
 aGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTATCATCAGTCTCAAACCGGCTCCAGAt                                                                                                                                                    <  1:694684/149‑1 (MQ=255)
 aGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGAt                                                                                                                                                    <  1:1058562/149‑1 (MQ=255)
 aGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGAt                                                                                                                                                    <  1:1119132/149‑1 (MQ=255)
   attggttttgtaggCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATag                                                                                                                                                  >  1:789498/14‑149 (MQ=255)
         tgtTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGCCTCAAACCGGCTCCAGAtgaagacgg                                                                                                                                            >  2:303019/1‑141 (MQ=255)
            ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggttt                                                                                                                                         <  2:179314/149‑1 (MQ=38)
            ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttt                                                                                                                                         >  2:1015672/1‑149 (MQ=255)
              attgcGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATCAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCACTCTCAAACCGGCTCTATCTGGAGCCggtttga                                                                                                                                       <  2:889228/145‑3 (MQ=38)
              aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttga                                                                                                                                       <  1:780432/149‑3 (MQ=38)
              aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATGAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCATTCTCAAACCGGCTCTATCTGGAGCCggtttga                                                                                                                                       <  2:889277/149‑3 (MQ=38)
               tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttgg                                                                                                                                      >  2:164122/1‑149 (MQ=255)
                  cGGATTCTGCGTTAACGCCTTATCCGGCCTACAAACCCAATTAAATTCAATGCATTAAAAATTGAGGTAGACCTGATAGATAGCACAACAGGCTATGTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggttt                                                                                                                                   <  2:748803/149‑1 (MQ=255)
                   ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggtttt                                                                                                                                  <  1:1148710/149‑1 (MQ=255)
                         ggcgTTAACGCCTTATCCGGCTCACAAAACCAATTAAATTCCATGCCTTCATCGCTCTTGTAGACGTGTTCCATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGTGCCGGTTTTGGTTTTCTGTCt                                                                                                                            <  1:236538/149‑1 (MQ=255)
                         ggcgTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATGAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGACCCGGTTCTGGCTTTCTGTCt                                                                                                                            >  2:84794/1‑149 (MQ=255)
                          gcgTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCtt                                                                                                                           <  1:566996/149‑1 (MQ=255)
                            gTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTaa                                                                                                                         <  2:1042640/149‑1 (MQ=255)
                                 cGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGCGCCGGTTTTGGTTTTCTGTCTTAACGCAc                                                                                                                    <  2:11175/149‑1 (MQ=255)
                                 cGCCTAAACCGGCCAACAAAACCAAACAAAACCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTATCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCAc                                                                                                                    <  2:50202/149‑1 (MQ=255)
                                     ttttCCGGCCCACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATg                                                                                                                <  2:1008315/146‑1 (MQ=255)
                                     ttATCCGGCCTACAAAACCAATTACATTCAATGAATTAAAAAAGGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATg                                                                                                                <  1:412265/149‑1 (MQ=255)
                                       aTCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGTGTTTCTGTCTTAACGCACACTGCa                                                                                                              >  1:1067235/1‑149 (MQ=255)
                                          cGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACgg                                                                                                           >  1:652081/1‑149 (MQ=255)
                                          cGGCCTAAAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGGCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACgg                                                                                                           <  1:268670/149‑1 (MQ=255)
                                           ggCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACggg                                                                                                          <  2:965310/149‑1 (MQ=255)
                                            gCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCCTGCACGGgc                                                                                                         >  2:671676/1‑149 (MQ=255)
                                            gCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAACACAACAGGCTATTTTGCGTTTGGCATAAGTCTCAAACCGACTCCAGATAGAGCCGGATTTGGTTTTCTGTCTTAACGCACCCTGCACGGgc                                                                                                         >  2:605552/1‑149 (MQ=255)
                                              cTACAAAACCGATTAAATTCAATGAGTTAAAAGATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGgcgc                                                                                                       <  2:716102/149‑1 (MQ=255)
                                                aCAAAACCAATTAAATTCAATGACTTAAAAAATGATGTAGACCTCATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCTGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCtt                                                                                                     <  2:778579/149‑1 (MQ=255)
                                                  aaaaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGt                                                                                                   >  2:56430/1‑149 (MQ=255)
                                                   aaaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCCCGGGCGCTTGtt                                                                                                  >  1:1067108/1‑149 (MQ=255)
                                                    aaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTa                                                                                                 >  1:603781/1‑149 (MQ=255)
                                                    aaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTa                                                                                                 >  1:970740/1‑149 (MQ=255)
                                                     aCCAATTAAAGTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTAt                                                                                                <  1:809636/149‑1 (MQ=255)
                                                         aTTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGaa                                                                                            <  1:715974/149‑1 (MQ=255)
                                                          ttgaaTTAAAAGTATTAAAAAAGGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAAACGGCTCCAGCTAGGGCCGGTTTCGGTCTTCTGTCTTAATGCACCATTCACGGGCTCTTGTTATCGAAc                                                                                           <  2:421681/146‑1 (MQ=255)
                                                            aaaTTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATGTGACGGTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGt                                                                                         >  1:966927/1‑149 (MQ=255)
                                                                tCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccag                                                                                     <  2:370413/149‑1 (MQ=255)
                                                                 cAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccagc                                                                                    <  2:1067233/149‑1 (MQ=255)
                                                                  aaTGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccagcc                                                                                   >  1:290706/1‑149 (MQ=255)
                                                                    tGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCGGGCTTAACGCACCAGGCACGGGCGCTTGTTATCGAACGTcccgccag                                                                                 >  1:115484/1‑149 (MQ=255)
                                                                        ttAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAAt                                                                             <  2:918419/149‑1 (MQ=255)
                                                                        ttAAAAAATGATGTAGACCGGATACATAGCACAACAGGCTATTTTGCGTTCAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAAt                                                                             <  1:286080/149‑1 (MQ=255)
                                                                            aaaaTGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGa                                                                         >  1:167829/1‑149 (MQ=255)
                                                                            aaaaTGACGTAGCCCTGATACATAGCACAACCGGCTATTTTGCTTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGa                                                                         <  1:734272/149‑1 (MQ=255)
                                                                              aaTGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATa                                                                       <  2:997332/149‑1 (MQ=255)
                                                                                 gatgTCGACCTGTTACATCGCCCAACAGGCTATTTTGCGTTGGGCATCAGTCTCAAACCGGCTCCAGATAGGGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTg                                                                    <  2:551295/149‑1 (MQ=255)
                                                                                 gatgTAGCCTTGCTACATAGCACAACCGGCTATTTTGCGTTTCGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTg                                                                    <  2:357527/149‑1 (MQ=255)
                                                                                 gatgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTg                                                                    <  2:1222088/149‑1 (MQ=255)
                                                                                 gatgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTg                                                                    <  2:594211/149‑1 (MQ=255)
                                                                                  atgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGc                                                                   <  1:15744/149‑1 (MQ=255)
                                                                                   tgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTGCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCa                                                                  <  2:839259/149‑1 (MQ=255)
                                                                                   tgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCa                                                                  <  1:526987/149‑1 (MQ=255)
                                                                                   tgTAGACCAGATCCATATCACCACAGTCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTGGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCa                                                                  <  2:917978/149‑1 (MQ=255)
                                                                                    gTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCag                                                                 >  1:62174/1‑148 (MQ=255)
                                                                                        aCCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTccc                                                             >  2:865566/1‑149 (MQ=255)
                                                                                        aCCGGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTccc                                                             <  2:1141271/149‑1 (MQ=255)
                                                                                          cTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACTGGCTCCGGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATCCTGCATTCCcat                                                           >  2:806817/1‑149 (MQ=255)
                                                                                          cTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGACCGTCCAGCCCGGAATCAGATCCTGCATTCCcat                                                           >  2:1051911/1‑149 (MQ=255)
                                                                                          cTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcat                                                           <  2:1120482/149‑1 (MQ=255)
                                                                                          cTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACAGGCACCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAAAGTCCAGCCAGGAATCCGAGAAGTCATTCCcag                                                           >  2:32317/1‑148 (MQ=255)
                                                                                           tGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTccccca                                                          >  2:283169/1‑146 (MQ=255)
                                                                                                  tAGCACAACAGGCTGTTTTGCGTTTAGCATCAGTCTGAAACCGGCTCCAGGTAGAGCCAGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGGGCTTGTTATGGATGGTCCGGCGTGGGATCAGATTCTGCATTGCcatggctgcgt                                                   >  2:816440/1‑144 (MQ=255)
                                                                                                  tAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCACGAATCAGATACTGCATTCCCATCGCCTCGt                                                   >  2:171526/1‑149 (MQ=255)
                                                                                                     cacaACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGGACCATGCACGGGCGCTTGTTATCGAACGCCCAGTCATGAATCAGATACTGCATTCCCATCGCATCGTCAc                                                <  2:211732/149‑1 (MQ=255)
                                                                                                       caacaGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACACACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCAcgc                                              >  1:503644/1‑149 (MQ=255)
                                                                                                              ttATTTTGCGTTTTGCATCAGGCTCAAACCGGCTCGAGATAGAGGCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCaa                                       <  1:198183/148‑1 (MQ=255)
                                                                                                              cTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTACAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCaa                                       <  1:686317/149‑1 (MQ=255)
                                                                                                               tATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAg                                      <  2:136783/149‑1 (MQ=255)
                                                                                                                            gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGa                         <  1:580667/149‑1 (MQ=255)
                                                                                                                               tCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCCAGCCCGTGTTTCTGATAc                      >  1:1197926/1‑149 (MQ=255)
                                                                                                                               tCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCCAGCCCGGGTTTCTGATAc                      >  1:314821/1‑149 (MQ=255)
                                                                                                                               tCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATAc                      >  1:641291/1‑149 (MQ=255)
                                                                                                                               tCAGTCCCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATAc                      <  1:67061/149‑1 (MQ=255)
                                                                                                                                  gTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGc                   >  1:501391/1‑149 (MQ=255)
                                                                                                                                  aaccaaaaCCGGCTCTACCTGGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCCGGAATCAGATACTGCATTCCCATCGCATCGTGCCGCGCGCCACGCCCGTGTTTCTGAtgcggc                   >  2:81871/6‑144 (MQ=255)
                                                                                                                                     tcAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCGCGTGTTTCTGATACAGCTCa                >  1:1205978/1‑149 (MQ=255)
                                                                                                                                     tcAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACAGCATTCCCATCGCATCGTCACACGCGCCAAGCCAATGTTTCAGATACACCTCa                >  1:471541/1‑149 (MQ=255)
                                                                                                                                      cAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACCGCTCAt               >  1:314024/1‑149 (MQ=255)
                                                                                                                                       aaaCCGGCTCTATCTGGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTCATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCACATg              >  2:905254/1‑149 (MQ=255)
                                                                                                                                       aaaCCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTCATGCAGCTCATg              >  2:122271/1‑149 (MQ=255)
                                                                                                                                          ccGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGGGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCCCGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGc           >  1:43378/1‑149 (MQ=255)
                                                                                                                                           cGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCt          <  1:1015672/149‑1 (MQ=255)
                                                                                                                                            ggCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCtt         <  2:789498/149‑1 (MQ=255)
                                                                                                                                                   gATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACCCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCAc  >  1:586358/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAGTGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCAC  >  CP009273/2911231‑2911526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: