Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,911,381 A→G 7.4% intergenic (+209/+23) barA → / ← gudD hybrid sensory histidine kinase, in two‑component regulatory system with UvrY/(D)‑glucarate dehydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,911,3810AG 7.4% 211.7 / 7.5 81intergenic (+209/+23)barA/gudDhybrid sensory histidine kinase, in two‑component regulatory system with UvrY/(D)‑glucarate dehydratase 1
Reads supporting (aligned to +/- strand):  ref base A (34/41);  new base G (2/4);  total (36/45)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

TGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACTT  >  CP009273/2911234‑2911528
                                                                                                                                                   |                                                                                                                                                   
attggttttgtaggCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATag                                                                                                                                                    >  1:789498/14‑149 (MQ=255)
         ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggttt                                                                                                                                           <  2:179314/149‑1 (MQ=38)
         ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttt                                                                                                                                           >  2:1015672/1‑149 (MQ=255)
           attgcGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATCAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCACTCTCAAACCGGCTCTATCTGGAGCCggtttga                                                                                                                                         <  2:889228/145‑3 (MQ=38)
           aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCTATCTGGAGCCggtttga                                                                                                                                         <  1:780432/149‑3 (MQ=38)
           aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATGAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCATTCTCAAACCGGCTCTATCTGGAGCCggtttga                                                                                                                                         <  2:889277/149‑3 (MQ=38)
            tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttgg                                                                                                                                        >  2:164122/1‑149 (MQ=255)
               cGGATTCTGCGTTAACGCCTTATCCGGCCTACAAACCCAATTAAATTCAATGCATTAAAAATTGAGGTAGACCTGATAGATAGCACAACAGGCTATGTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggttt                                                                                                                                     <  2:748803/149‑1 (MQ=255)
                ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCggttttggtttt                                                                                                                                    <  1:1148710/149‑1 (MQ=255)
                      ggcgTTAACGCCTTATCCGGCTCACAAAACCAATTAAATTCCATGCCTTCATCGCTCTTGTAGACGTGTTCCATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGTGCCGGTTTTGGTTTTCTGTCt                                                                                                                              <  1:236538/149‑1 (MQ=255)
                      ggcgTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATGAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGACCCGGTTCTGGCTTTCTGTCt                                                                                                                              >  2:84794/1‑149 (MQ=255)
                       gcgTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCtt                                                                                                                             <  1:566996/149‑1 (MQ=255)
                         gTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTaa                                                                                                                           <  2:1042640/149‑1 (MQ=255)
                              cGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGCGCCGGTTTTGGTTTTCTGTCTTAACGCAc                                                                                                                      <  2:11175/149‑1 (MQ=255)
                              cGCCTAAACCGGCCAACAAAACCAAACAAAACCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTATCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCAc                                                                                                                      <  2:50202/149‑1 (MQ=255)
                                  ttttCCGGCCCACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATg                                                                                                                  <  2:1008315/146‑1 (MQ=255)
                                  ttATCCGGCCTACAAAACCAATTACATTCAATGAATTAAAAAAGGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATg                                                                                                                  <  1:412265/149‑1 (MQ=255)
                                    aTCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGTGTTTCTGTCTTAACGCACACTGCa                                                                                                                >  1:1067235/1‑149 (MQ=255)
                                       cGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACgg                                                                                                             >  1:652081/1‑149 (MQ=255)
                                       cGGCCTAAAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGGCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACgg                                                                                                             <  1:268670/149‑1 (MQ=255)
                                        ggCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACggg                                                                                                            <  2:965310/149‑1 (MQ=255)
                                         gCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCCTGCACGGgc                                                                                                           >  2:671676/1‑149 (MQ=255)
                                         gCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAACACAACAGGCTATTTTGCGTTTGGCATAAGTCTCAAACCGACTCCAGATAGAGCCGGATTTGGTTTTCTGTCTTAACGCACCCTGCACGGgc                                                                                                           >  2:605552/1‑149 (MQ=255)
                                           cTACAAAACCGATTAAATTCAATGAGTTAAAAGATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGgcgc                                                                                                         <  2:716102/149‑1 (MQ=255)
                                             aCAAAACCAATTAAATTCAATGACTTAAAAAATGATGTAGACCTCATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCTGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCtt                                                                                                       <  2:778579/149‑1 (MQ=255)
                                               aaaaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGt                                                                                                     >  2:56430/1‑149 (MQ=255)
                                                aaaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCCCGGGCGCTTGtt                                                                                                    >  1:1067108/1‑149 (MQ=255)
                                                 aaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTa                                                                                                   >  1:970740/1‑149 (MQ=255)
                                                 aaCCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTa                                                                                                   >  1:603781/1‑149 (MQ=255)
                                                  aCCAATTAAAGTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTAt                                                                                                  <  1:809636/149‑1 (MQ=255)
                                                      aTTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGaa                                                                                              <  1:715974/149‑1 (MQ=255)
                                                       ttgaaTTAAAAGTATTAAAAAAGGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAAACGGCTCCAGCTAGGGCCGGTTTCGGTCTTCTGTCTTAATGCACCATTCACGGGCTCTTGTTATCGAAc                                                                                             <  2:421681/146‑1 (MQ=255)
                                                         aaaTTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATGTGACGGTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGt                                                                                           >  1:966927/1‑149 (MQ=255)
                                                             tCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccag                                                                                       <  2:370413/149‑1 (MQ=255)
                                                              cAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccagc                                                                                      <  2:1067233/149‑1 (MQ=255)
                                                               aaTGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTccagcc                                                                                     >  1:290706/1‑149 (MQ=255)
                                                                 tGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCGGGCTTAACGCACCAGGCACGGGCGCTTGTTATCGAACGTcccgccag                                                                                   >  1:115484/1‑149 (MQ=255)
                                                                     ttAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAAt                                                                               <  2:918419/149‑1 (MQ=255)
                                                                     ttAAAAAATGATGTAGACCGGATACATAGCACAACAGGCTATTTTGCGTTCAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAAt                                                                               <  1:286080/149‑1 (MQ=255)
                                                                         aaaaTGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGa                                                                           >  1:167829/1‑149 (MQ=255)
                                                                         aaaaTGACGTAGCCCTGATACATAGCACAACCGGCTATTTTGCTTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGa                                                                           <  1:734272/149‑1 (MQ=255)
                                                                           aaTGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATa                                                                         <  2:997332/149‑1 (MQ=255)
                                                                              gatgTCGACCTGTTACATCGCCCAACAGGCTATTTTGCGTTGGGCATCAGTCTCAAACCGGCTCCAGATAGGGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTg                                                                      <  2:551295/149‑1 (MQ=255)
                                                                              gatgTAGCCTTGCTACATAGCACAACCGGCTATTTTGCGTTTCGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTg                                                                      <  2:357527/149‑1 (MQ=255)
                                                                              gatgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTg                                                                      <  2:594211/149‑1 (MQ=255)
                                                                              gatgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTg                                                                      <  2:1222088/149‑1 (MQ=255)
                                                                               atgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGc                                                                     <  1:15744/149‑1 (MQ=255)
                                                                                tgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTGCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCa                                                                    <  2:839259/149‑1 (MQ=255)
                                                                                tgTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCa                                                                    <  1:526987/149‑1 (MQ=255)
                                                                                tgTAGACCAGATCCATATCACCACAGTCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTGGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCa                                                                    <  2:917978/149‑1 (MQ=255)
                                                                                 gTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCag                                                                   >  1:62174/1‑148 (MQ=255)
                                                                                     aCCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTccc                                                               >  2:865566/1‑149 (MQ=255)
                                                                                     aCCGGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTccc                                                               <  2:1141271/149‑1 (MQ=255)
                                                                                       cTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACTGGCTCCGGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATCCTGCATTCCcat                                                             >  2:806817/1‑149 (MQ=255)
                                                                                       cTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGACCGTCCAGCCCGGAATCAGATCCTGCATTCCcat                                                             >  2:1051911/1‑149 (MQ=255)
                                                                                       cTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCcat                                                             <  2:1120482/149‑1 (MQ=255)
                                                                                       cTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACAGGCACCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAAAGTCCAGCCAGGAATCCGAGAAGTCATTCCcag                                                             >  2:32317/1‑148 (MQ=255)
                                                                                        tGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTccccca                                                            >  2:283169/1‑146 (MQ=255)
                                                                                               tAGCACAACAGGCTGTTTTGCGTTTAGCATCAGTCTGAAACCGGCTCCAGGTAGAGCCAGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGGGCTTGTTATGGATGGTCCGGCGTGGGATCAGATTCTGCATTGCcatggctgcgt                                                     >  2:816440/1‑144 (MQ=255)
                                                                                               tAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCACGAATCAGATACTGCATTCCCATCGCCTCGt                                                     >  2:171526/1‑149 (MQ=255)
                                                                                                  cacaACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGGACCATGCACGGGCGCTTGTTATCGAACGCCCAGTCATGAATCAGATACTGCATTCCCATCGCATCGTCAc                                                  <  2:211732/149‑1 (MQ=255)
                                                                                                    caacaGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACACACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCAcgc                                                >  1:503644/1‑149 (MQ=255)
                                                                                                           ttATTTTGCGTTTTGCATCAGGCTCAAACCGGCTCGAGATAGAGGCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCaa                                         <  1:198183/148‑1 (MQ=255)
                                                                                                           cTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTACAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCaa                                         <  1:686317/149‑1 (MQ=255)
                                                                                                            tATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAg                                        <  2:136783/149‑1 (MQ=255)
                                                                                                                         gCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGa                           <  1:580667/149‑1 (MQ=255)
                                                                                                                            tCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCCAGCCCGTGTTTCTGATAc                        >  1:1197926/1‑149 (MQ=255)
                                                                                                                            tCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCCAGCCCGGGTTTCTGATAc                        >  1:314821/1‑149 (MQ=255)
                                                                                                                            tCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATAc                        >  1:641291/1‑149 (MQ=255)
                                                                                                                            tCAGTCCCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATAc                        <  1:67061/149‑1 (MQ=255)
                                                                                                                               gTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGc                     >  1:501391/1‑149 (MQ=255)
                                                                                                                               aaccaaaaCCGGCTCTACCTGGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCCGGAATCAGATACTGCATTCCCATCGCATCGTGCCGCGCGCCACGCCCGTGTTTCTGAtgcggc                     >  2:81871/6‑144 (MQ=255)
                                                                                                                                  tcAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCGCGTGTTTCTGATACAGCTCa                  >  1:1205978/1‑149 (MQ=255)
                                                                                                                                  tcAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACAGCATTCCCATCGCATCGTCACACGCGCCAAGCCAATGTTTCAGATACACCTCa                  >  1:471541/1‑149 (MQ=255)
                                                                                                                                   cAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACCGCTCAt                 >  1:314024/1‑149 (MQ=255)
                                                                                                                                    aaaCCGGCTCTATCTGGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTCATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCACATg                >  2:905254/1‑149 (MQ=255)
                                                                                                                                    aaaCCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTCATGCAGCTCATg                >  2:122271/1‑149 (MQ=255)
                                                                                                                                       ccGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGGGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCCCGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGc             >  1:43378/1‑149 (MQ=255)
                                                                                                                                        cGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCt            <  1:1015672/149‑1 (MQ=255)
                                                                                                                                         ggCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCtt           <  2:789498/149‑1 (MQ=255)
                                                                                                                                                gATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACCCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCAc    >  1:586358/1‑149 (MQ=255)
                                                                                                                                                  tAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACtt  <  1:746706/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCCTGTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAACCAATTAAATTCAATGAATTAAAAAATGATGTAGACCTGATACATAGCACAACAGGCTATTTTGCGTTTAGCATCAGTCTCAAACCGGCTCCAGATAGAGCCGGTTTTGGTTTTCTGTCTTAACGCACCATGCACGGGCGCTTGTTATCGAACGTCCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACTT  >  CP009273/2911234‑2911528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: