Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,911,664 A→G 8.2% intergenic (‑39/+12) atpG ← / ← atpA F1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,911,6640AG 8.2% 223.5 / 8.8 88intergenic (‑39/+12)atpG/atpAF1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (42/38);  new base G (5/3);  total (47/41)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.48e-01

TGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCG  >  CP009273/3911519‑3911811
                                                                                                                                                  |                                                                                                                                                   
tGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGAGCTTTTGAGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgcc                                                                                                                                                  >  2:806432/1‑149 (MQ=255)
 gCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGgccg                                                                                                                                                 <  1:857556/149‑1 (MQ=255)
    aTTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGgccgcaa                                                                                                                                              <  1:1117476/149‑3 (MQ=255)
    aTTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCa                                                                                                                                              >  1:1235731/1‑149 (MQ=255)
     tttACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGGCCGCAAg                                                                                                                                             <  2:131668/149‑1 (MQ=255)
     tttACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGAAAACCGCCAg                                                                                                                                             >  1:1170274/1‑149 (MQ=255)
           cATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTCCGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCGGCCCTAAGGCAGGCCGCCAgcgctta                                                                                                                                       >  1:670448/1‑143 (MQ=255)
              tttGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCGTAAGGCAAGCCGCCAGACGTTACCa                                                                                                                                    <  1:622186/149‑1 (MQ=255)
               ttGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAg                                                                                                                                   >  1:271477/1‑149 (MQ=255)
                     gcggcgACCATCTCCATCGCTTTAGTGGTCTTTTGCGTGTTCAG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTgg                                                                                                                             <  2:1163036/149‑1 (MQ=255)
                     gcggcgACCATCTCCAGCGCCTTAGTGACCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTgg                                                                                                                             <  1:267438/149‑1 (MQ=255)
                      cggcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTATC‑GGCGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCCGACGTTACCAGGATTggg                                                                                                                            >  1:177335/1‑149 (MQ=255)
                        gcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGCTCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGtt                                                                                                                          >  1:169699/1‑149 (MQ=255)
                        gcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGtt                                                                                                                          >  1:974439/1‑149 (MQ=255)
                        gcgACCATCTCCATCGCTTTAGTGAGCTTTAGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGtt                                                                                                                          >  1:640783/1‑149 (MQ=255)
                         cgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTg                                                                                                                         <  1:1114199/149‑1 (MQ=255)
                              atgtgcATCGCTTTAGTGTTCTTTTGCGTGTTCTG‑TACGCTTGCGATCTTACTACGTATCTCTTTGGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGGGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTg                                                                                                                    <  2:986480/144‑1 (MQ=255)
                               tctcCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGa                                                                                                                   <  2:825623/149‑1 (MQ=255)
                                tcccATCTCTGTAGTTTTCTTTTGCCGGTTCTT‑GACGCTTGCGATCTAACTACGTATCTCTTTTGCGCCGTCCATGCGCTTCTCCTCGATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGaa                                                                                                                  <  2:63394/147‑1 (MQ=255)
                                ctctATCGCCTTAGCGATGTGTTGGCTGGTCTT‑GACGCTTGCGATCTTACTACGTTTCTTCTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCGTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGaa                                                                                                                  <  2:549082/149‑1 (MQ=255)
                                 tcCATCGCTTTAGTGCTCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGAATTGGGTTGCTTTGAAg                                                                                                                 >  2:143806/1‑149 (MQ=255)
                                 tcCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAg                                                                                                                 >  1:211235/1‑149 (MQ=255)
                                 tcCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAACCCGCCAGACGTTACCAGGCTTGGGTTGCTTTGAAg                                                                                                                 >  2:498956/1‑149 (MQ=255)
                                  ccATCGCTTTAGTGATCTTTTGCGAGTTATG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATTAGATTCTGCGCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAgg                                                                                                                >  1:926594/1‑149 (MQ=255)
                                   cATCGCTTTAGTGATCTTTTGCGTGAAATGAGAAG‑TTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGa                                                                                                               >  1:1226397/1‑149 (MQ=255)
                                        cTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAAttgg                                                                                                          >  1:167340/1‑146 (MQ=255)
                                        cTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGa                                                                                                          <  1:857455/149‑1 (MQ=255)
                                           tcgTGATCTTTTGCGTGTTATG‑GACGCTTGCGATCTTACTACGTGTCTCTTTTGCGCCGGCCGTGAGCTTCTCCTCAATGCCTTGCGGCGTGCGCTAAGGCAAGGCGCCAGACGTTGCCAGGGTTGGGTTGCTTTGAAGTGATCGAGGa                                                                                                       >  2:212281/3‑149 (MQ=255)
                                           tAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTCAGGCAAGCCGCCACACGTTACCAGGCTTGGGTTGCTTTGAAGGACACGAGGa                                                                                                       >  2:469569/1‑149 (MQ=255)
                                           tAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAggg                                                                                                       >  2:1131692/1‑148 (MQ=255)
                                               taTCTTTTGCCGTTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGcc                                                                                                   <  2:57171/148‑1 (MQ=255)
                                                aTCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGTGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTACCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCt                                                                                                  >  1:837474/1‑149 (MQ=255)
                                                aTCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCt                                                                                                  >  1:74014/1‑149 (MQ=255)
                                                aTCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGGATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCt                                                                                                  >  1:728615/1‑149 (MQ=255)
                                                    tttGCGTGTTCTG‑CACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCCCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCa                                                                                              <  1:26151/149‑1 (MQ=255)
                                                        cGTTTTCTG‑GACGCTTGCGATCTTACTACGTATCCCTTGTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTAAAAAAAAACGAAGGAATCGAGGATGCCTTTCAGCtt                                                                                          <  2:269117/149‑1 (MQ=255)
                                                        cGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCtt                                                                                          >  1:929730/1‑149 (MQ=255)
                                                          tgtTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGc                                                                                        <  1:767330/149‑1 (MQ=255)
                                                              cgg‑gACGCTTGCGTTTTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTc                                                                                    <  1:1028478/147‑1 (MQ=255)
                                                                  gACGCTTGCGATCTTATTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCTTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAt                                                                                 <  1:8335/149‑1 (MQ=255)
                                                                     tcTTTCGATTTTATTCCGTTTTTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATCCCTTGCGGCCTGCCCTAAGTTAAGCTGTCACACGTTACCATGATTGCGTTGCTTTGAAGGAATCGAGTATTCCTTTCAGCTTGCCTTCGATTtc                                                                              <  2:666203/148‑1 (MQ=255)
                                                                     gCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtc                                                                              <  2:395690/149‑1 (MQ=255)
                                                                      cTTGCGATCTTACTACGTACCTCTTTTGCGCCGGCCATGATCTTCTCCCCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTTGATTtcg                                                                             <  2:842314/149‑1 (MQ=255)
                                                                         tcGACCTTACTACGTATCTCCTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTGCAGCTTGCCTTCGATTtcgtcg                                                                          <  2:167396/148‑1 (MQ=255)
                                                                         gCGATCTTACTACGTATCTCTTTTGCGGCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCGAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCATTTCAGCTTGCCTTCGATTtcgtcg                                                                          <  1:619695/149‑1 (MQ=255)
                                                                         gCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcgtcg                                                                          <  1:1006427/149‑1 (MQ=255)
                                                                         gCGATCTTACTACGTATCTCTGTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcgtcg                                                                          <  1:505105/149‑1 (MQ=255)
                                                                          cGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTcgtcgt                                                                         <  1:112521/149‑1 (MQ=255)
                                                                          cGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTcgtcgt                                                                         <  1:79844/149‑1 (MQ=255)
                                                                            aTCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCGCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGGTTGCCTTCGATTTCGTCGTTg                                                                       >  2:266813/1‑149 (MQ=255)
                                                                               ttACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGGCGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGtgg                                                                    >  2:133178/1‑147 (MQ=255)
                                                                               ttACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAg                                                                    >  1:461820/1‑149 (MQ=255)
                                                                                  ctacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCa                                                                 <  2:974439/149‑1 (MQ=255)
                                                                                     cGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCg                                                              <  1:926815/149‑1 (MQ=255)
                                                                                           tctTTTGCGCCGGCCATGAGCTTCTCCGCAAGGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTgg                                                        <  1:205955/149‑1 (MQ=255)
                                                                                             ttttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGtt                                                      >  1:584570/1‑149 (MQ=255)
                                                                                             ttttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGtt                                                      >  2:771461/1‑149 (MQ=255)
                                                                                             ttttGCGCCGGCCATGAGCTTCACCTCAATGACTTGCGGCCTGCACTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGCAGGAATCGAGGCAGCCGTTCCGCTTGCCTTCGATTTCGGCGTGGTAGCCACCGGGCTGGtg                                                      >  2:929729/1‑148 (MQ=255)
                                                                                              tttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCCGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGGTTGCCTTCGATTTCGTCGTTGTAGCCACCGGGCTGGTTg                                                     >  1:373723/1‑149 (MQ=255)
                                                                                               ttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGa                                                    >  1:217633/1‑149 (MQ=255)
                                                                                                tGCGCCCGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGAt                                                   <  2:493981/149‑1 (MQ=255)
                                                                                                   gcCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCAGCGGGCCGGTTGAtctc                                                >  1:188006/1‑149 (MQ=255)
                                                                                                     cGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAGGCCGCCAGACGTTACCGGGGTTGGGCTGCTTTGAAGGAATCGAGGAGGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCCCCGGTCTGCTTGATCTCtt                                              >  2:949954/1‑149 (MQ=255)
                                                                                                        tcATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCa                                           <  1:178740/148‑1 (MQ=255)
                                                                                                        ccctGAGCTTCCTCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTCGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCa                                           <  1:254071/146‑1 (MQ=255)
                                                                                                           tGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAAGACGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTAATCTCTTGCATCa                                        >  1:239258/1‑149 (MQ=255)
                                                                                                           tGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATGGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCCCTTGCATCa                                        >  2:28230/1‑149 (MQ=255)
                                                                                                             aacTTCGCCGCAATGCCTTGCGGCCGGCCGTAAGGGCAGCCGCCAGACGTTACGAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTCTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGGTGATCTCTTGCATCAAc                                      <  2:916400/147‑1 (MQ=255)
                                                                                                                  ctcctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGc                                 <  2:584570/149‑1 (MQ=255)
                                                                                                                     ctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTTATCTCTTGCATCAACGGAGCGTg                              >  1:1105266/1‑149 (MQ=255)
                                                                                                                      tcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTCGTAACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGa                             >  1:1152506/1‑149 (MQ=255)
                                                                                                                        aaTGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCGTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATc                           <  1:824019/149‑1 (MQ=255)
                                                                                                                           gCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCAACGGTCTGGGTGATCTCTTGCGTCAACGGAGCGTGATCACg                        >  1:679464/1‑149 (MQ=255)
                                                                                                                            ccTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGGCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGACAACgg                       >  2:922953/1‑149 (MQ=255)
                                                                                                                            ccTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAAACGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGCTCTCTTGCATCAACgggcagtgagaccgg                       >  1:834615/1‑136 (MQ=255)
                                                                                                                             cTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGt                      <  2:1226397/149‑1 (MQ=255)
                                                                                                                             cTTGCGGCCTGCCCTAAGGCAAGCCGCCACACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGt                      <  2:271477/149‑1 (MQ=255)
                                                                                                                               ggCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCg                    >  1:1142785/2‑149 (MQ=255)
                                                                                                                                gCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGAGCACGGTCGa                   >  1:98212/1‑149 (MQ=255)
                                                                                                                                 cGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGCTCACGGTCGAc                  >  2:629972/1‑149 (MQ=255)
                                                                                                                                 cGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGAc                  <  2:24142/149‑1 (MQ=255)
                                                                                                                                  ggccggccCTAAGGCAAGCCGCCAGACGTTACCAGGATGGGGTAGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTGGCCACCGGTCTGGTTGATCTCTTGCTTCAACGGAGCGTGATCACGGTCGACg                 <  2:641892/149‑1 (MQ=255)
                                                                                                                                  ggCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACg                 >  2:584223/1‑149 (MQ=255)
                                                                                                                                          cTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGTATCAACGGAGCGTGATCACGGTCGACGTAAGCcag         <  2:772474/149‑1 (MQ=255)
                                                                                                                                          cTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGCCGTAAGCcag         >  1:1067033/1‑149 (MQ=255)
                                                                                                                                          cTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGACGACGTAAGCcag         >  1:519408/1‑149 (MQ=255)
                                                                                                                                          cTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTGCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGGCGTAAGCcag         >  1:1183071/1‑149 (MQ=255)
                                                                                                                                              ggCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCaga     >  1:149234/1‑149 (MQ=255)
                                                                                                                                                 aaGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCg  >  1:1095256/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTG‑GACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCG  >  CP009273/3911519‑3911811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑