Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,394,665 T→G 29.3% H54Q (CAT→CAG purA → adenylosuccinate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,394,6650TG29.3% 68.2 / 41.9 64H54Q (CAT→CAGpurAadenylosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base T (25/20);  new base G (10/9);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.73e-01

TCGTCGTACTGGGCACCCAATGGGGTGACGAAGGTAAAGGTAAGATCGTCGATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCAT  >  CP009273/4394517‑4394813
                                                                                                                                                    |                                                                                                                                                    
tcgtcgtACGGGGCACCCAATGGGGTGACGAAGGTAAAGGTAAGATCGTCGATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCAt                                                                                                                                                      <  1:1120606/149‑1 (MQ=255)
  gtggcaatGGGCCCCCAATGGGGTGACGAAGGTAAAGGTAAGGCCGTCGATCTTCTGACTGAACGGGCTAAATAGGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCCCGTAATCAACGGTGAAAAAACCGTTCTCCATCt                                                                                                                                                    <  1:573350/142‑1 (MQ=255)
    cgtACTGGGCACCCAATGGGGTGACGAAGGTAAAGGTAAGATCGTCGATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTa                                                                                                                                                  <  2:126726/149‑1 (MQ=255)
            tcACCCCATTGCGTGACGAAGGTAAAGGTAAGATCGTCGATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCa                                                                                                                                          >  2:729660/2‑149 (MQ=255)
                ccAATGGGGTGTCGAAGGTAAAGGTAAGATCGCCGATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTa                                                                                                                                      <  1:52539/149‑1 (MQ=255)
                ccAAAGGGGAGCCGTTGGTACAGGTAAGATCGTCGATTATCTCACTGAACGGGCTAAATATGTCGTACGCGACCTGGGCGTTCACAACGCACGCCATACTCTCGTAAAAAAAAAAAAAAAAACCGTTCTCCATCTTATTCCATCAGGTa                                                                                                                                      <  2:291249/149‑1 (MQ=255)
                   aTGGGGTGACGAAGGTAAAGGTAAGAGCGTCGATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATtc                                                                                                                                   >  2:630075/1‑149 (MQ=255)
                    tGGGGTGACGAAGGTAAAGGTAAGATCGTCGATCTTCTGACTGAACGGGCTGAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCCCCATCTTATTCCACCAGGTATtcc                                                                                                                                  >  1:399707/1‑148 (MQ=255)
                          gACGAAGGTAAAGGTAAGATCTCCGATCGGGTGACGGAACGGCCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAAAAAAAAAAAAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCga                                                                                                                            <  2:131339/149‑1 (MQ=255)
                           aCGAAGGTAAAGGTAAGATCGTCGATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCgag                                                                                                                           >  1:225398/1‑149 (MQ=255)
                              aaggtaaaggtaAGATCGTCGATCTTCTGACTGAACGGGCTAAATATGTCGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAAt                                                                                                                        >  2:183891/1‑149 (MQ=255)
                                  taaaggtagGATCGTCGGTCTTCGGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATCCTCTCGTAATCAACGGTGAAAAAACTGTCGGCCTGCGGATTCCATCAGGTATTCTCCGCGAGAATGTaa                                                                                                                    <  1:1224368/149‑1 (MQ=255)
                                       gtaaGATCGTCGATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGc                                                                                                               >  2:997306/1‑149 (MQ=255)
                                            aTCGTCGATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGGGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGcatcat                                                                                                          >  2:1229696/1‑149 (MQ=255)
                                               gtcgATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCgg                                                                                                       >  1:206899/1‑149 (MQ=255)
                                               gtcgATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCgg                                                                                                       >  1:638470/1‑149 (MQ=255)
                                                   aTCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTCTTATCCGCGAGAATGTAACCAGCATCACCGGTAAc                                                                                                   >  2:870636/1‑149 (MQ=255)
                                                   aTCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAAc                                                                                                   >  1:994817/1‑149 (MQ=255)
                                                   aTCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGAATCATCGGTAAc                                                                                                   >  1:586650/1‑149 (MQ=255)
                                                   aTCTTCTGACTAAACGGGCTAAATATGTTCTACGAATGCAGTTCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCGGATATTCTCCGCGAGAATGTAACAAGCATCCTCGGTAAc                                                                                                   >  2:574769/1‑149 (MQ=255)
                                                            ctgaACGGGCTAAGTTCGTTGTACGCGCCCAGGGCGGCCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTtgtg                                                                                          <  2:338167/149‑1 (MQ=255)
                                                              gaACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCt                                                                                        <  2:910640/149‑1 (MQ=255)
                                                                   ggCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGAGTTGTGCttgatc                                                                                   >  2:66452/1‑144 (MQ=255)
                                                                   ggCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGtctc                                                                                   >  1:117972/1‑149 (MQ=255)
                                                                   ggCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGtctc                                                                                   >  1:422606/1‑149 (MQ=255)
                                                                       aaaTATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGc                                                                               <  1:409590/149‑1 (MQ=255)
                                                                         atatGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCcg                                                                             <  2:1226331/149‑1 (MQ=255)
                                                                              ttgtACGCTACCAGGGCGGTCACAACGCACGCCATACTCTCGTAATCAACGGTGAAAAAACCGCTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGAGATGGGCTGCCCCCGGgcgccgtg                                                                        >  2:931294/1‑145 (MQ=255)
                                                                                gtACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCtgat                                                                      >  1:1142854/1‑149 (MQ=255)
                                                                                gtACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCtgat                                                                      <  1:729660/149‑1 (MQ=255)
                                                                                    gCTACCAGGGCGGTCACAACGCGGGCCATCCTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGTTgatgaaa                                                                  <  2:1004486/149‑1 (MQ=255)
                                                                                    gCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGGGCTgatgaaa                                                                  >  2:925577/1‑149 (MQ=255)
                                                                                    gCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGCTGAAAAAACCGTTCTCCATCTTATTCCATCCGGCATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGCGTTGTGCTGTCTCCGGGCGCGCTgatgcaa                                                                  >  2:132361/1‑149 (MQ=255)
                                                                                    gCTACCAGGCCGGTCACAACGCAGGCCATCCCCCCGTAATCAACGGTGAAAAAACCGTTCTCCAGCTTATTCCATCCGGTATTCTCCGCGAGAAGGTAACCCGCATCATCGGTAGCGGGGTTGTGCTGTCTCCGGCCGCGCtgacgcac                                                                  >  2:1097299/1‑144 (MQ=255)
                                                                                     cTACCAAGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGGGAAAAAACCATTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGATAACGGTGTTGTGCTGTCTCCGGCCGCGCTgatgaaag                                                                 >  2:949518/1‑149 (MQ=255)
                                                                                         cAGGGCGGTCACAACGCAGGCCCTACTCTCGTAATCCACGGTGAAAAAACCGTTCTCCATATTATTACAACAGGTGTTCTCCGCGAGAATATAACCAGAATCACCCGTCACGGTGTTGTGCGGTCTCCTGCAGCGCTgcggaaagagca                                                             >  2:525927/1‑147 (MQ=255)
                                                                                          aGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCCCGCTgatgaaagggatg                                                            >  1:361300/1‑149 (MQ=255)
                                                                                          aGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCACGCGAATGTAACCATCATCGTCGGTAACGGTGTTGTGCTGTCTTCGGCCGCGCTgatgaaagagatg                                                            >  2:951428/1‑149 (MQ=255)
                                                                                            ggCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGGATGTAACCAGCATCATCGTTAATGGTGTTGTGCTGTCTCCGGCCGCGCTgatgaaagagatgaa                                                          <  1:485690/149‑1 (MQ=255)
                                                                                            ggCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAATGTACCCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTgatgaaagagatgaa                                                          <  2:205128/149‑1 (MQ=255)
                                                                                                gtccaaaCGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCAGCTTATTCCATCAGGTATGCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGaa                                                      <  1:16304/144‑1 (MQ=255)
                                                                                                gTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGATGTCTCCGGCCGCGCTGATGAAAGAGATGCAAGaa                                                      >  2:592051/1‑149 (MQ=255)
                                                                                                 taacaACGCAGGCCATACCCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATGCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAAc                                                     <  1:759313/147‑1 (MQ=255)
                                                                                                 tCACAACGCAGGCCATACTTTCGTAACCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAAc                                                     <  2:166107/149‑1 (MQ=255)
                                                                                                    caACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCAGCAACGGTGTTGTGCTGTCTCGGGCCGCGCTGATGAAAGAGAGGAAAGAACTgg                                                  >  2:684852/1‑149 (MQ=255)
                                                                                                     aaCGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGCTGAAAGCGATGAAAGAAGTGGa                                                 >  1:702630/1‑149 (MQ=255)
                                                                                                     aaCGCAGGCCATACTCTCGCAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTggc                                                 >  1:455697/1‑148 (MQ=255)
                                                                                                      aCGCAGGCCATACTCTCGGAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGaa                                                <  1:558168/149‑1 (MQ=255)
                                                                                                              cATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTgg                                        <  1:515122/149‑1 (MQ=255)
                                                                                                                         aaTCAACGGTGAAAAAACCGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTc                             <  1:1133393/149‑1 (MQ=255)
                                                                                                                            cAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTg                          <  1:754535/149‑1 (MQ=255)
                                                                                                                              aCGGTGAAAAAACCGTTCTCCATCTTTTTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAg                        <  2:399707/149‑1 (MQ=255)
                                                                                                                              aCGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAg                        <  1:410503/149‑1 (MQ=255)
                                                                                                                                 gtaaaaaacCCGGTCTCCCGCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATCACCGAGATGAAACAACTGGTAGACCGTGGCATCGCCGTTCTTGTGCGt                     <  2:1224512/146‑1 (MQ=255)
                                                                                                                                 gTGAAAAAGCCGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGt                     <  2:971988/149‑1 (MQ=255)
                                                                                                                                  tGAAAAAACCGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTc                    >  2:632545/1‑149 (MQ=255)
                                                                                                                                   gAAAAAACCGTTCTCCCTCCTATTCCCTCAGGGATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTct                   <  1:468886/149‑1 (MQ=255)
                                                                                                                                    aaaaaaCCGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTctg                  >  2:737441/1‑149 (MQ=255)
                                                                                                                                    aaaaaaCCGTTCCCCATCCTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTctg                  <  1:48141/149‑1 (MQ=255)
                                                                                                                                         tccGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTctgctgct             <  2:701008/148‑1 (MQ=255)
                                                                                                                                         aCCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGGAGACCGTGGCATCCCCGGTCGTGAGCGTctgctgct             >  1:268922/1‑149 (MQ=255)
                                                                                                                                           cGTTCTCCAGCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATAGGTAACGGTGTTGTGCTGTGTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGt           >  1:1198573/1‑149 (MQ=255)
                                                                                                                                              tctcCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTg        <  1:51579/149‑1 (MQ=255)
                                                                                                                                                tcCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGaa      >  1:49497/1‑149 (MQ=255)
                                                                                                                                                 ccATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAGGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGCAg     >  1:455153/1‑149 (MQ=255)
                                                                                                                                                    tCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCAt  >  2:1011538/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCGTCGTACTGGGCACCCAATGGGGTGACGAAGGTAAAGGTAAGATCGTCGATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCAT  >  CP009273/4394517‑4394813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: