Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,406,114 A→T 5.3% intergenic (‑5/+144) yjfN ← / ← bsmA DUF1471 family periplasmic protein/bioflm peroxide resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,406,1140AT 5.3% 198.2 / 2.0 69intergenic (‑5/+144)yjfN/bsmADUF1471 family periplasmic protein/bioflm peroxide resistance protein
Reads supporting (aligned to +/- strand):  ref base A (34/31);  new base T (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01

CCCTGAAATATTGCTAACTGAGATCTGACCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAGCAATGTAAAGCTGACTATGTAAGTTGTCTGATTTA  >  CP009273/4405966‑4406260
                                                                                                                                                    |                                                                                                                                                  
cccTGAAATATTGCTAACTGAGATCTGACCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGGATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGCGGGTGAGGCAAGTAATTGTTTCATCGTCa                                                                                                                                                    >  1:663994/1‑149 (MQ=255)
 ccTGAAATATTGCTAACTGAGATCTGACCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGTCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAg                                                                                                                                                   >  1:244368/1‑149 (MQ=255)
         atTGCTGACTGAGATCCGACCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCAttt                                                                                                                                           <  2:80209/149‑1 (MQ=255)
         actccTAACTGAGATCTGACCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCAttt                                                                                                                                           <  2:990259/145‑1 (MQ=255)
           tGCTAACTTAGATCTGACTTATTACATTCAGGCCCGTCACGCAGTCATGCCTGGGCAATTCTTCAGACCGTTCCTTTGCGCTTGCAGAATTAGTTACCAATTGCAGCTAGGGTGAGGCAAGTAATTCTTTCCTTGATAGCTCCAttttt                                                                                                                                         <  2:150887/149‑1 (MQ=255)
             cTAACTGAGATCTGACCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGGGAGGCATGTAATTGTTTCATCGTCAGCTCCCTTTTTCg                                                                                                                                       >  2:1202467/1‑149 (MQ=255)
                  tGAGATCTGACCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTGTCGTTTcc                                                                                                                                  >  1:158888/1‑149 (MQ=255)
                    agaTCTGACCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCACGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTcccc                                                                                                                                >  2:357061/1‑149 (MQ=255)
                      aTCTTACCTATTTCATTCAGGCCCGTCACGCAGTCAGCGCTTGCGAATTCTGCAGACTGTGCCTTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGATGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCcac                                                                                                                              <  2:435409/149‑1 (MQ=255)
                              tATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCa                                                                                                                      <  1:810786/149‑1 (MQ=255)
                              tATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCa                                                                                                                      <  1:1197509/149‑1 (MQ=255)
                                tttCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAgg                                                                                                                    >  1:813966/1‑149 (MQ=255)
                                tttCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAgg                                                                                                                    >  1:500897/1‑149 (MQ=255)
                                   cattcaGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCa                                                                                                                 <  1:762203/149‑1 (MQ=255)
                                   cattcaGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGCGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCa                                                                                                                 <  1:569946/149‑1 (MQ=255)
                                     ttcaGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGCCTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCTGCTCCATTTTTCGTTTCCCCCCATGTTGCAGGGCAtt                                                                                                               >  2:68210/1‑149 (MQ=255)
                                     ttcaGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCTGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCAtt                                                                                                               >  2:408083/1‑149 (MQ=255)
                                             cGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTGTTCGTTTCCCCACATGTTGCAGGGCATTCCATcgcg                                                                                                       >  1:154173/1‑149 (MQ=255)
                                                 aCGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATAGTCAGCTACATTTTTCGTTTCATCGCATGGTTCAGGGCAGTCCAGCTCGGtaa                                                                                                   <  2:1036319/149‑1 (MQ=255)
                                                    gaGTAAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCACGTCGAATAACTCAGTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGtaataa                                                                                                <  1:711197/148‑1 (MQ=255)
                                                     aGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCAATTTTGGGAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATaaa                                                                                               <  1:1213695/149‑1 (MQ=255)
                                                         aGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCGCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCA‑TTTTCGTTTCCCCACATGTTGCAAGGCATTCCATCGCGGTACTAAACTccc                                                                                          <  2:1197190/149‑1 (MQ=255)
                                                          gCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCCCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGccc                                                                                          <  1:1043330/149‑1 (MQ=255)
                                                           cACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGCGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCAACGCCCGACGGGCATTCCAGCGCGGGAGTAAGCGCCCa                                                                                         >  1:285883/1‑149 (MQ=255)
                                                           cACTGGCGAAGTCTGCGGCCTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGCGGGGCAAGTAATTGTTTCATCGGAAGCGCCATTTTTCGTTGCGCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCa                                                                                         >  2:327168/1‑149 (MQ=255)
                                                             cgggCTAAATCCGCAGACAGGGGCGTGGCGCTAGCAAGATAATTCACTAATGGCATCGAGGGTCAGGCAAGTAAGTGTTGCATCGTCAGCTCCACTTTTCGTTTCCCCACATGTTGCAGGGCATGCCATCGCGGTAATAAACGCCCAgg                                                                                       <  2:745374/147‑1 (MQ=255)
                                                                gCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACCTGTTGCAGGGCATTCCATCGCGGTAATAACCGGCACGGCtt                                                                                    >  2:395964/1‑149 (MQ=255)
                                                                gCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTACATCGCGGTAATAAACGCCCAGGCtt                                                                                    >  2:159670/1‑149 (MQ=255)
                                                                     ttCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGc                                                                               >  1:213609/1‑149 (MQ=255)
                                                                     ttCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACAGGTGGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGc                                                                               <  2:1129662/149‑1 (MQ=255)
                                                                     ttCTGCAGACGGGGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAACCGCCCAGGATTGCAGc                                                                               <  2:940258/149‑1 (MQ=255)
                                                                       cTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCCCCATTTTTCGTTTCCCCACATGTTGCAGGGCCTTCCATCGCGGTCGTAAAGGGCCAGGCTgccggccg                                                                             >  2:310679/1‑149 (MQ=255)
                                                                        tGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTccggccgg                                                                            >  1:976545/1‑149 (MQ=255)
                                                                        tGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCgg                                                                            >  1:976639/1‑149 (MQ=255)
                                                                         gCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGt                                                                           >  2:440639/1‑149 (MQ=255)
                                                                          cAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTa                                                                          >  2:143722/1‑149 (MQ=255)
                                                                             aCTGTGCCGTCGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGGGAGGCAAGGAATTGTTTCATCGTCAGCTGCATTTTTCGTTTCCCCCCATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAAtt                                                                       <  2:144507/149‑1 (MQ=255)
                                                                                   ccGTGGCGCTCGCAGGATAAGTCACTAATGGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATtgt                                                                 <  2:677214/149‑1 (MQ=255)
                                                                                   ccGTGGCCCTCGCAGGATAAGTCACTAATTGAAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATtgt                                                                 <  1:420850/149‑1 (MQ=255)
                                                                                        tgcgtCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGcaca                                                            >  2:783765/5‑149 (MQ=255)
                                                                                        gcgcTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGcaca                                                            <  1:1042158/149‑1 (MQ=255)
                                                                                          gcTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAgg                                                          >  2:1060364/1‑149 (MQ=255)
                                                                                              gCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGGATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTaaa                                                      >  1:567018/1‑149 (MQ=255)
                                                                                              gCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTTATTTATTGTGCACAGGTcaa                                                      >  1:129381/1‑149 (MQ=255)
                                                                                                     aaGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATTTTGCAGGGCATTCCATCGCGGTAGAAAACGCCCAGGATTACGGCCGGGAGATTATTGGGCAGAGGTtaaaagaaag                                               >  2:253267/1‑142 (MQ=255)
                                                                                                     aaGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAg                                               <  1:1060364/149‑1 (MQ=255)
                                                                                                     aaGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTGGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAg                                               <  1:260239/149‑1 (MQ=255)
                                                                                                            tAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCa                                        <  1:1022700/149‑1 (MQ=255)
                                                                                                               ttGCAGCGAGGGTGGGGCAAGTATTTGTTTCATCGTCAGCTCCTTTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAg                                     <  1:39573/149‑1 (MQ=255)
                                                                                                                 gCAGGGAGGGTGAGCCGAGTAAGCGTTTCATCGGCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCAACGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAGCa                                   <  1:1032261/149‑1 (MQ=255)
                                                                                                                    gCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCGGCCGGTAATTTATTGTTCACAGGTAAACGCATACGAGCGCAAAGCACTg                                >  1:325715/1‑149 (MQ=255)
                                                                                                                      gAGGGTGAGGCACGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCTCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAATCAATGTa                              >  2:146213/1‑149 (MQ=255)
                                                                                                                        gggTGAGGCAAGTAATTGTTTCATCGTCTGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAGCAATGTaaa                            <  1:68210/149‑1 (MQ=255)
                                                                                                                        gggTGAGGCAAGTAATTGTTTCATCGTCTGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAGCAATGTaaa                            <  1:408083/149‑1 (MQ=255)
                                                                                                                         ggTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTACACGCATAGGGACGCAAAGCCATGttacg                           >  2:799381/1‑145 (MQ=255)
                                                                                                                         ggTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCACAGCAATGTAAAg                           <  2:1169413/149‑1 (MQ=255)
                                                                                                                                cAAGTAATTGTTTCATCGTCAGCTCCATTTTTTTTTTTTTTTCATGTCGCAGGCCGTTCCCTCGCGATAATACACGGACAGGGCTCCAGCCGGGGATTTATTGTGCACACGTAAACGCATAGCCGCGGCAAGCAATGCaaatttgatga                    >  2:356987/1‑141 (MQ=255)
                                                                                                                                cAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAACCGCCCAGGCTTCCCGCCGGTAATTTATTGTGCACGGGTAAACGCATGGGGACGCACAGCAATGTAAAGCTGCCTa                    >  1:339072/1‑149 (MQ=255)
                                                                                                                                    tAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATCGTGCACAGGTAAACGAATAGGGACGCAAAGCAATGTAAAGCTGACCATGTa                >  1:779543/1‑149 (MQ=255)
                                                                                                                                     aaTTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAGCAATGTAAAGCTGACTATGTaa               <  1:1057794/149‑1 (MQ=255)
                                                                                                                                       ttGTTTCATCGTCAGCTCCATTTTTCGTTTCCTTTCATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTTTTGTGCACAGGTAAACGCATAGGGACGCAAAGCAATGTAAAGTTTACTATGTGAGt             >  2:774207/1‑149 (MQ=255)
                                                                                                                                        tGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAGCAATGTAAAGCTGACTATGTAAGtt            <  2:244368/149‑1 (MQ=255)
                                                                                                                                        tGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAGCAATGTAAAGCTGACTATGTAAGtt            >  1:795932/1‑149 (MQ=255)
                                                                                                                                          tttCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAGCAATGTAAAGCTGACTATGTAAGTTGt          >  1:1026879/1‑149 (MQ=255)
                                                                                                                                           ttCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAGCAATGTAAAGCTGACTATGTAAGTTGTc         >  2:1040420/1‑149 (MQ=255)
                                                                                                                                            tCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAGCAATGTAAAGCTTACTATGTAAGTTGTCt        <  1:783765/149‑1 (MQ=255)
                                                                                                                                            tCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAGCAATGTAAAGCTGACTATGTAAGTTGTCt        <  1:440639/149‑1 (MQ=255)
                                                                                                                                               tCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAGCAATGTAAAGCTGACTATGTAAGTTGTCTGat     <  2:394390/149‑1 (MQ=255)
                                                                                                                                               tCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGACTAGGGCCGCAAAGCAATGTAAAGCTGACTAGGTAAGTTGGCTGat     >  2:495208/1‑149 (MQ=255)
                                                                                                                                                  tCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAGCAATGTAAAGCTGACTATGTAAGTTGTCTGatttg  >  1:650930/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCCTGAAATATTGCTAACTGAGATCTGACCTATTTCATTCAGGCCCGTCACGCAGTCAGCACTGGCGAATTCTGCAGACTGTGCCGTGGCGCTCGCAGGATAAGTCACTAATTGCAGCGAGGGTGAGGCAAGTAATTGTTTCATCGTCAGCTCCATTTTTCGTTTCCCCACATGTTGCAGGGCATTCCATCGCGGTAATAAACGCCCAGGCTTCCAGCCGGTAATTTATTGTGCACAGGTAAACGCATAGGGACGCAAAGCAATGTAAAGCTGACTATGTAAGTTGTCTGATTTA  >  CP009273/4405966‑4406260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑