BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,050,832	G→A	S188L (TCG→TTG)	glnA ←	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,050,832	0	G	A	99.0%	322.5 / NA	103	S188L (TCG→TTG)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (54/48); total (55/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

AATTTCGTCAGCTTTTTTGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATCCGA  >  CP009273/4050687‑4050973

aaTTTCGTCAGCTTTTTTGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGt                                                                                                                                            <  1:812292/149‑1 (MQ=255)

    tCGTCAGCTTTTTTGGTCATGGTATTGAAG‑AGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTAcc                                                                                                                                       <  2:1227694/149‑1 (MQ=255)

    tCGCCAGCTTTTTTGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTAc                                                                                                                                        <  1:81882/149‑1 (MQ=255)

       tCAGCTTTTTTGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCgg                                                                                                                                     >  1:1008378/1‑149 (MQ=255)

        taGCTTTTTTGGTCACGGTATTGAAGCGGGGAGCCACGTCGTTCTGACCAGCAGTCTCGACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGt                                                                                                                                    <  2:369275/148‑1 (MQ=255)

           cTTTTTTGGTCATGGTATTGACGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCCGTCCCATTTCAGAACGAATATCCTGAGCAAAGTCTACCGGTGGa                                                                                                                                 >  1:694735/1‑149 (MQ=255)

           cTTTTTTGGTCATGGTATTGAAGCGGGTAGCCACTGCGGTCTGACCAGCAGTCGCTACTTCGTGGTGATGAGCTTCAACCACCAGACCCATCGGTTCGTTCACCAGACACATTTAAGCACGAATATCCTGAGCCAAGTCTACGGGTGGa                                                                                                                                 >  1:129321/1‑149 (MQ=255)

            ttttttGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCCCCAGACACATTTCAGAACGAATATCCTGGGCCAAGTCTACCGGTGGaa                                                                                                                                >  2:739923/1‑149 (MQ=255)

            ttttttGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACCTTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTggga                                                                                                                                >  2:740028/1‑147 (MQ=255)

                 tGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCGGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCggg                                                                                                                           >  1:1146331/1‑149 (MQ=255)

                 tGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCggg                                                                                                                           <  2:514175/149‑1 (MQ=255)

                   gTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGaa                                                                                                                         <  2:135948/149‑1 (MQ=255)

                   gTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGaa                                                                                                                         <  2:761070/149‑1 (MQ=255)

                   gTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGaa                                                                                                                         <  2:252311/149‑1 (MQ=255)

                    tCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAg                                                                                                                        >  2:816380/1‑149 (MQ=255)

                    tCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCGGAGCCAAGTCCACCGGTGGAGCCGGGAAg                                                                                                                        >  2:584693/1‑149 (MQ=255)

                     cATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGt                                                                                                                       <  2:1093834/149‑1 (MQ=255)

                      aTGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGACGCTACTTCGAGGCAATGGGTTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATACTGAGCCAAGTCTACCGGTGACACCGGGAAGTa                                                                                                                      >  2:483214/1‑149 (MQ=255)

                       tGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTCGAACCGGGGAGTaa                                                                                                                     >  2:285076/1‑149 (MQ=255)

                        ggTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAAc                                                                                                                    >  1:542234/1‑149 (MQ=255)

                           atggaggCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCATGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGc                                                                                                                 <  2:189157/143‑1 (MQ=255)

                           aTTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGc                                                                                                                 >  1:1209438/1‑149 (MQ=255)

                           aTTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTGCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCTTCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGc                                                                                                                 <  2:262553/149‑1 (MQ=255)

                           aTTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCCACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGc                                                                                                                 <  2:262517/149‑1 (MQ=255)

                             tGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCt                                                                                                               <  1:450359/149‑1 (MQ=255)

                              gAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCtt                                                                                                              >  2:406283/1‑149 (MQ=255)

                               aaGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACGGCCttt                                                                                                             >  1:764727/1‑149 (MQ=255)

                               aaGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCttt                                                                                                             >  2:1111784/1‑149 (MQ=255)

                                 gCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTTCTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCa                                                                                                           >  2:846383/1‑149 (MQ=255)

                                 gCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCa                                                                                                           <  2:866918/149‑1 (MQ=255)

                                 gCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCa                                                                                                           <  2:1179012/149‑1 (MQ=255)

                                   gggTAGCCACTTCGTTCTGACCAGCAGCCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACt                                                                                                         <  1:161932/149‑1 (MQ=255)

                                     gTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCAACTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGACGTAACCGCCTTTCACTGc                                                                                                       >  1:409025/1‑149 (MQ=255)

                                          cACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTGACCGCCTTTCACTGCCGGAc                                                                                                  >  1:1174880/1‑149 (MQ=255)

                                          cACTTCGTTCTGACCAGCAGTCGCTACACCCTCGCCAGTTTCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGAc                                                                                                  >  1:1311923/1‑149 (MQ=255)

                                           aCTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACg                                                                                                 >  1:836037/1‑149 (MQ=255)

                                           aCTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACg                                                                                                 >  2:524812/1‑149 (MQ=255)

                                              tCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTg                                                                                              <  2:495930/149‑1 (MQ=255)

                                                 ttCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGAcc                                                                                           >  2:479557/1‑149 (MQ=255)

                                                     gACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGAAACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGCGCGCTTTg                                                                                       >  1:330457/1‑149 (MQ=255)

                                                       ccAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTATCACTGCCGGACGGTGACCTTTGtt                                                                                     <  1:669847/149‑1 (MQ=255)

                                                        cagcagTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTa                                                                                    >  1:1121138/1‑149 (MQ=255)

                                                        cagcagTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTa                                                                                    >  1:137978/1‑149 (MQ=255)

                                                        cagcagTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACAAATCAGAACGAATATCCTGAGCCAAGTCTACCGGAGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTa                                                                                    >  1:559111/1‑149 (MQ=255)

                                                         agcagTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGATCTTTGTTac                                                                                   <  1:228527/149‑1 (MQ=255)

                                                          gcagTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCACCGGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGATCCGGGAAGTAACCGCCTTTCACTGCCAGACGGCGACCCTTGTTacc                                                                                  >  2:409929/1‑149 (MQ=255)

                                                           cagTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTacca                                                                                 <  2:1137833/149‑1 (MQ=255)

                                                              tCGCTGCTTCGTGGTGATGTGCTTTAACCGCCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCt                                                                              <  2:396706/149‑1 (MQ=255)

                                                              tCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTaaacaat                                                                              >  2:988580/1‑143 (MQ=255)

                                                                gCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCCTCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGCCCTTTGGTACCACCTTc                                                                            <  2:818773/149‑1 (MQ=255)

                                                                gCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTc                                                                            <  1:820160/149‑1 (MQ=255)

                                                                       cGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTgg                                                                     >  2:1062763/1‑149 (MQ=255)

                                                                        gtggtgATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACAGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACTTTCGCATTggg                                                                    >  1:497452/1‑149 (MQ=255)

                                                                        gtggggAGGGGCTTCCACCACGAGACCCATCTGTTCCATCACCAGACACCATTCAGAACGAATATCCTGGGCCAAGTCTAGCGGTGGAACCGGGAAGTAACCGTCTTTCACTGCCGGACGGTGACCTTTGTTACCAACTTCGTATTggg                                                                    <  2:491151/149‑1 (MQ=255)

                                                                         tggtgATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTgggg                                                                   >  2:180964/1‑148 (MQ=255)

                                                                          ggtgATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTCACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTg                                                                  <  1:1016192/149‑1 (MQ=255)

                                                                          ggtgACGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTg                                                                  <  1:722607/149‑1 (MQ=255)

                                                                             gATGGGCTCCAACCACCAGGCCGCGCTGTTCCCTCACCAGACACCTTGCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTggag                                                               <  2:1161179/149‑1 (MQ=255)

                                                                              aTGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACATTCGTGTTGGGTggagg                                                              >  2:584668/1‑149 (MQ=255)

                                                                                gggCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGgaggag                                                            >  2:101832/1‑149 (MQ=255)

                                                                                 ggCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTCCGTATTGGGTGGAGGAGt                                                           <  1:974708/149‑1 (MQ=255)

                                                                                  gCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGtt                                                          <  1:239604/149‑1 (MQ=255)

                                                                                  gCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGtt                                                          <  2:3957/149‑1 (MQ=255)

                                                                                   cTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGACGTAACCGCCTTTCACTGCCGGACGGGTGCCTTTGTGACCACCTTCGTACTGGGTGGAGGAGTTc                                                         >  2:417463/1‑149 (MQ=255)

                                                                                     tCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGCGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCGTTCACTCCCGGACGGTGACCTTTGTTCCCACCTTCGGCTTGGGGGGAGGAGTTGCa                                                       >  2:621223/1‑149 (MQ=255)

                                                                                      cacccaccaGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCAt                                                      <  2:394582/146‑1 (MQ=255)

                                                                                       aaCCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATg                                                     <  1:1005126/149‑1 (MQ=255)

                                                                                        accaccAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTGCCTgc                                                    >  1:451181/1‑149 (MQ=255)

                                                                                        accaacAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATgc                                                    <  1:479557/149‑1 (MQ=255)

                                                                                          caccaGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTTCCGGTCGCTGACCCTTTTTACCACCTTCTTATTGGGTGGAGGTGTTCCCTgcgc                                                  >  2:352088/1‑149 (MQ=255)

                                                                                          caccaGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATgcgc                                                  >  1:1186884/1‑149 (MQ=255)

                                                                                            ccaGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCt                                                >  2:427609/1‑149 (MQ=255)

                                                                                              aGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTc                                              >  2:1154471/1‑149 (MQ=255)

                                                                                               gACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGGCCTTGGTTACCACCTGCGTATTGGGTGGAGGAGTTCCATGCGCCTTCg                                             >  2:47333/1‑149 (MQ=255)

                                                                                               gACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCg                                             <  1:78414/149‑1 (MQ=255)

                                                                                                 cccATCTGTCCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGat                                           <  2:129321/149‑1 (MQ=255)

                                                                                                   cATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGGGTTCGATGCGCCTTCgcaat                                         >  2:137118/1‑145 (MQ=255)

                                                                                                    aTCTGTTCCATCACCAGACACATGTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGTGGAGTTCCATGCGCCTTCGATAtc                                        <  1:988580/149‑1 (MQ=255)

                                                                                                        gTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCGTTCACTGTCGTACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATAtcgtcg                                    <  2:478587/149‑1 (MQ=255)

                                                                                                        gTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCGACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATGGGGTGGAGGAGTTCCATGTGCCTTCGATAtcgccg                                    >  2:876180/1‑149 (MQ=255)

                                                                                                            cATTACCAGACACATTTCAGTACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAg                                <  1:351511/149‑1 (MQ=255)

                                                                                                            cATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGACCCGGGAAGTAACCGCCTTTCACTCCCGGACCGTGACGTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAg                                <  1:584693/149‑1 (MQ=255)

                                                                                                            cATCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGATGGCACCGGGAAGGAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAg                                >  2:220448/1‑149 (MQ=255)

                                                                                                             aTCACCAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGAAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGc                               >  2:854850/1‑149 (MQ=255)

                                                                                                                  cAGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACAGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGt                          >  2:536035/1‑149 (MQ=255)

                                                                                                                   aGACACATTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGCCGGTGACCTTTGTTACCACCTTCGTATTGGTTGGAGGAGTTGCATGGGCCTTCGGTATCGTGGATTGCGACGTg                         >  2:238266/1‑149 (MQ=255)

                                                                                                                      cacaTTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGa                      >  2:1120719/1‑149 (MQ=255)

                                                                                                                       acaTTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGGGGAGGAGTTCCATGCGCCTTCGATATCGTCCATAGCAACGCGGGaa                     >  1:1018439/1‑149 (MQ=255)

                                                                                                                        caTTTCAGAACGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAAc                    <  1:406283/149‑1 (MQ=255)

                                                                                                                               gAACGAATATCCTGAGCCAAGTCTCCCGGTGGCACCGGGAAGTAACCGCCTCTGACCGCAGGACCGTGGACTCTGTTACCACCTTCGTACTGGGTGGCGGGGCTCCTTGCGCCTTCGATATCGTCGAGACGAGGGTGGGAACCGGagat             >  2:615049/1‑149 (MQ=255)

                                                                                                                                aaCGAATATTCTGAGCCAAGTCTAACGGTGGAACCGGGAAGGAAGCGTCTTGCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGagata            <  1:366215/149‑1 (MQ=255)

                                                                                                                                 aCGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCTTCGATAGCAACGTGGGAACCGGagatag           <  1:797430/149‑1 (MQ=255)

                                                                                                                                  cGAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGagataga          <  1:1053405/149‑1 (MQ=255)

                                                                                                                                   gAATATCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGagatagat         >  1:1160097/1‑149 (MQ=255)

                                                                                                                                      tatCCTGAGCCAAGTCTACCGGTGGAACCGGCAAGGAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCGACGTGGGAACCGGAGATAgatgat      <  1:670778/149‑1 (MQ=255)

                                                                                                                                        tCCTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATcc    <  1:524812/149‑1 (MQ=255)

                                                                                                                                         ccTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATCCg   <  1:740028/149‑1 (MQ=255)

                                                                                                                                          cTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATCCGa  >  2:81218/1‑149 (MQ=255)

                                                                                                                                          cTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGGGGGAACCGGGGATAGATGATCCGa  >  2:105597/1‑149 (MQ=255)

                                                                                                                                          cTGAGCCAAGTCTACCGGTGGAACCGGGAAGTAACCGCCTATCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGCGGAGTTCCATGCGCCTTCCATATCGTCGCTAGCCACGTGGCAGCCGGAGATAGATGAGCCGa  >  2:992286/1‑149 (MQ=255)

AATTTCGTCAGCTTTTTTGGTCATGGTATTGAAGCGGGTAGCCACTTCGTTCTGACCAGCAGTCGCTACTTCGTGGTGATGGGCTTCAACCACCAGACCCATCTGTTCCATCACCAGACACATTTCAGAACGAATATCCTGAGCCGAGTCTACCGGTGGAACCGGGAAGTAACCGCCTTTCACTGCCGGACGGTGACCTTTGTTACCACCTTCGTATTGGGTGGAGGAGTTCCATGCGCCTTCGATATCGTCGATAGCAACGTGGGAACCGGAGATAGATGATCCGA  >  CP009273/4050687‑4050973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`