New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3528142	146 (0.850)	4 (0.020)	4/496	15.0	2.7%	coding (1674/2136 nt)	yrfF	putative RcsCDB‑response attenuator, inner membrane protein
	?	NC_000913	= 3528152	145 (0.870)					coding (1684/2136 nt)	yrfF	putative RcsCDB‑response attenuator, inner membrane protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3527855‑3528142 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcgccgtttCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAGATTGACCAGCGCATTTTTCAGTCGCACACAGTCATCTTTGGCAGAACATAAATCCGCTGTCTTCAGTACAATGTCGCCAAAATCATCAAGCAATACCATGCCGGATTTTTGAATTGCTGAACGTAATGAGGCA  <  NC_000913/3528152‑3527875                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        GAAGATGACTCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAACAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAAAGCGCCACCGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCACGGTGAGCGCGGACTCGCTGGATAACCTGGTGGCAACCTCCACAGC                                                                                                                                                                                                                                                                                               >  1:1105969/1‑281         ATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCAGGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAACCGCGCCAACGCACGCAAGCTCGATGGCGTGAATGTTTTATTACTCGCCGTGATTCCGGAATCACTGGTTAACCTGGTGGCAACCCTCACCGCCCCcgtta                                                                                                                                                                                                                                                                                       >  2:257707/1‑276                   AGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCG                                                                                                                                                                                                                                                                                              >  1:817318/1‑264                   AGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCG                                                                                                                                                                                                                                                                                              <  2:817318/264‑1                                                                                                                                                                                                                                                     CGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGC                                                                                                                                                                 <  1:1524060/163‑1                                                                                                                                                                                                                                                     CGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGC                                                                                                                                                                 >  2:1524060/1‑163                                                                                                                                                                                                                                                                          TGGCAACCTCCCCCGCGACGGTCCATGCGGGGTCGACGGCGCGGTTGAGGTTGCCACCAGCTTAGCCAGCGATTCCGCACTCACCGGGCGTAATGACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAGATTGAACAGCGCATTTTTCAGTCGCACACAGTCATCTTTGGCAGAACATAAATCCGCTGTCTTCAGTACAATGTCGCCAAAATCATCAAGCAATACCATGCCGGATTTTTGAA                      <  1:1178834/281‑1                                                                                                                                                                                                                                                                                    CACCGCGCCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAGATTGACCAGCGCATTTTTCAGTCGCACACAGTCATCTTTGGCAGAACATAAATCCGCTGTCTTCAGTACAATGTCGCCAAAATCATCAAGCAATACCATGCCGGATTTTTGAATTGCTGAACG            >  2:319291/1‑281                                                                                                                                                                                                                                                                                       CGCGCCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAGATTGACCAGCGCATTTTTCAGTCGCACACAGTCATCTTTGGCAGAACATAAATCCGCTGTCTTCAGTACAATGTCGCCAAAATCATCAAGCAATACCATGCCGGATTTTTGAATTGCTGAACG            >  1:1444015/1‑278                                                                                                                                                                                                                                                                                        GCGCCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTGTACTGTTGCCGAGATTGACCAGCGCATTTTTCAGTCGCACACAGTCAGTTTTGGCAGAACATAAATCCGCTGTCTTCAGTACAATGTCGCCAAAATCATCAAGCAACACAATGCCGGATTTTTGAATTGATGAACGTAAT        >  2:1280085/1‑281                                                                                                                                                                                                                                                                                          GCCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAGATTGACCAGCGCATTTTTCAGTCGCACACAGTCATCTTTGGCAGAACATAAATCCGCTGTCTTCAGTACAATGTCGCCAAAATCATCAAGCAATACCATGCCGGATTTTTGAA                      >  1:782499/1‑265                                                                                                                                                                                                                                                                                          GCCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAGATTGACCAGCGCATTTTTCAGTCGCACACAGTCATCTTTGGCAGAACATAAATCCGCTGTCTTCAGTACAATGTCGCCAAAATCATCAAGCAATACCATGCCGGATTTTTGAA                      <  2:782499/265‑1                                                                                                                                                                                                                                                                                          GCCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAGATTGACCAGCGCATTTTTCAGTCGCACACAGTCATCTTTGGCAGAACATAAATCCGCTGTCTTCAGTACAATGTCGCCAAAATCAT                                                 >  1:1584118/1‑238                                                                                                                                                                                                                                                                                          GCCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAGATTGACCAGCGCATTTTTCAGTCGCACACAGTCATCTTTGGCAGAACATAAATCCGCTGTCTTCAGTACAATGTCGCCAAAATCAT                                                 <  2:1584118/238‑1                                                                                                                                                                                                                                                                                           CCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAGATTGACCAGCGCATTTTTCAGTCGCACACAGTCATCTTTGGCAGAACATAAATCCGCTGTCTTCAGTACAATGTCGCCAAAATCATCAAGCAATACCATGCCGGATTTTTGAATTGCTGAACGTAATGAG     <  2:1255352/281‑1                                                                                                                                                                                                                                                                                              TTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAGATTGACCAGCGCATTTTTCAGTCGCACACAGTCATCTTTGGCAGAACATAAATCCGCTGTCTTCAGTACAATGTCGCCAAAATCATCAAGCAATACCATGCCGGATTTTTGAATTGCTGAACGTAATGAGGCA  >  1:578794/1‑281                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        GAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3527855‑3528142 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcgccgtttCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAGATTGACCAGCGCATTTTTCAGTCGCACACAGTCATCTTTGGCAGAACATAAATCCGCTGTCTTCAGTACAATGTCGCCAAAATCATCAAGCAATACCATGCCGGATTTTTGAATTGCTGAACGTAATGAGGCA  <  NC_000913/3528152‑3527875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.