| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,123,175 | C→T | 6.1% | T55T (ACG→ACA) | ftsN ← | essential cell division protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,123,175 | 0 | C | T | 6.1% | 258.6 / 4.5 | 93 | T55T (ACG→ACA) | ftsN | essential cell division protein |
| Reads supporting (aligned to +/- strand): ref base C (39/45); new base T (3/3); total (43/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
CTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCC > NC_000913/4122896‑4123456 | gtGTTCCGGCGTCTGTTCCTTCCACGGAACTTCAGCCAGCTTCGTTGGCTGTTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGGTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCg < 2:247605/280‑1 (MQ=255)cTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCg < 1:558247/281‑1 (MQ=255) tGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCg < 2:981315/280‑1 (MQ=255) tGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCg > 1:981315/1‑280 (MQ=255) ttCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGGGTTGGCTGCTGGGGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACGTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTa < 2:1058048/280‑1 (MQ=255) ttCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCCTCTCGGACTCTTCTTTCTTGTGATGCGTaa > 1:483137/1‑281 (MQ=255) cGGCGTGTTGTCATTCCACGGGAAGTCAACCAGCTGGGTGGGCTGCTGGCGCATATCGGCCTGCATTTGTTACAGAAGCTGACGTTGTTCTGGTGTCAGTGGCTCCGGCTTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCGCTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCGTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATg < 2:1029349/280‑1 (MQ=255) ggcggcTGTTCATTCCCCGGAACTTCAACCGGCGGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATg < 1:983396/279‑1 (MQ=255) tGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCCCTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCGGCGTGCTTCTGGGTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCGCGGACGCTGCTTTCTTGTGATGCGTAATTAAGTACa > 2:983396/1‑280 (MQ=255) ttCATTCCGCGGAACTTCAGCTAGCTGTTTTGGCTGGTGGGGCACATCAGACTGCTGTTGTTCAAGAAGCTGACGTTGGTCTGGTGTCGGTTGCGCCGGCGTTAGCACTTCGCCACGGGCAGAAGGTTCTG‑TGCGCGTACGCACTCCCGGCTGGCGACTTTCCAGCTCGTTGATGT‑AGCGCGAGCGTTTTTCTGGTTTTGGGGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGTGTAATGAAGTACAGac < 2:763970/281‑1 (MQ=255) cATTCCACGGAAGTTCAAACAGCTGCGTTGGCTGCTGGCGCAGATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGacc < 1:49754/280‑1 (MQ=255) gaCTTCGACGAGCTTCGGTGGCTGTTGGCGCAGTGCAGCGTGGGTTTTTTCAGGGGACTGTCGTTGTTCGTGTGTGATTTGCTGTGGCGTTTTCGATTCACCGCCGGC‑GAAGGGTTTGATGGGCGACCGGGCTCCCGGCTTGCGACTTTCCAGCTCTGTGATGT‑GGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGTTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTAATGCGTAATGAGGGACAGACCACCGATAAAg < 2:264851/279‑1 (MQ=255) cAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGGTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACGCTTCTTTCTCGTGATGGGTAATGAAGTCAACACCACCGATAAAGGTcaca > 2:13565/1‑281 (MQ=255) tGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTCCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAAcggc < 1:139786/281‑1 (MQ=255) cGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAAcggcgg < 1:264886/281‑1 (MQ=255) gctgGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATa > 1:1026083/1‑281 (MQ=255) tgGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGacc > 1:383595/1‑245 (MQ=255) tgGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGacc < 2:383595/245‑1 (MQ=255) tgGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTGTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTCCAGACCACAGATAAAGGTCACAAGAACGGCGGCCGCAATAg > 1:558238/1‑280 (MQ=255) tatCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGacc > 2:580963/1‑238 (MQ=255) tatCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGacc < 1:580963/238‑1 (MQ=255) ccTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTGTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGTCTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGCTAAAGGTCGCAAGAACGGCGGCAGCAAAACGGACCATAGCggg > 2:371393/1‑280 (MQ=255) gttttttgtaaagcagCTGAGGGTGTTGTGGTGTTGGTTGCTCCGGGGTTTTCATTTCACCACCCGCGGAAGGTTCTG‑GGGGCGCACGCACTCCCGGGTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTTGTGGTATTCCGGTTCCGGTCACTTTCTGGCTGTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGgaga < 2:885595/270‑1 (MQ=255) cATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTCCTGGTGTTGGTGGTAGTCCGTTCCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACCACTCTTTCTTGTGATGCGTAATGAAGTACAGACAACCGATAAAGGTACCAAAAACGGCGGCAGCAATAGCGCCCAGAGCGGGAGaaa > 2:452451/1‑281 (MQ=255) ttCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCgca > 2:478903/1‑280 (MQ=255) cTGACGGTGTTTTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCATCGTTATTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTc < 2:916712/281‑1 (MQ=255) gACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGt < 1:478903/281‑1 (MQ=255) aGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGTCGACTTTCCAGCTCTTTCATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGT‑ATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGCTTTCGTTGCTTTTTCCGTGAGGTg > 1:1018802/1‑281 (MQ=255) aGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCTGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACATGAACGGCGGCAGCAATAGCGACAATAGCGGGAGAAACTGCAGGCAGATTTCGTTGTGTTTTCCGCGAGGt > 2:410323/1‑281 (MQ=255) gCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACGCTTCTTTCTTGTGATTCGTAATGAAGTACAGACCACCGATAAAGGTCAAAAGAACGGCGGCCGCAATAGCCACCATAGCGGGAGAAACCGTCGGCAGCGTTGGGTGCTTTTTCAGGGGGGGGCCAtttttt > 2:374501/1‑281 (MQ=255) agttcgcCGCCGGCAGGAGGTTGTA‑GGGGCGCTCAGAATCCCGGATGAGCACTTTCAGGCTCTTGAAGTT‑GGGGCCGACGTTCTTTGGGTGTTGGGGGTAGTCCGGTTGCGGTCACTTTCGGGCTTGGCAGCGTCTCGGACTGTGCTTTCTTGTGATGAGTAATGAAGTACAGACCACCGATAGAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCcgcg < 2:61671/278‑1 (MQ=255) ctgcccccccGGGAGCAGGTTGTG‑TGGTGGAACGCACTGCCGGCTGGCGACGTTCCAGCTCTGTAATGT‑AGCGACGGCGTTTTTCTGGTTTGTGTGGTAGTCCGTTTCCGGTCACTTTCTGACTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGa < 2:746227/272‑1 (MQ=255) accaccGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTg > 1:360071/1‑281 (MQ=255) cGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCCGATTTCGATGCTTTTTTCGTGAGGTGCTCTTTTTTG‑GCCGCGAAGGTGCCGGt > 1:326681/1‑281 (MQ=255) aaGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTTGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTg < 1:695723/281‑1 (MQ=255) ggTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGGCTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCTGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACATGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTgcg < 1:410323/281‑1 (MQ=255) cTG‑TGCGCGCGCACCCTCCCTGCTCGCGACTTTCCAGC‑GTTTGACGTCAGCGCCACCTTTTTTCTGGTTTTGTTGGACTTCCGTTTCCGGTCAGTTGCGGGCTGTGCAGGGTCTCGGACTCTGCTTTCTTGTTGTGCGTAATGAGGGACAAACCACCGATAAAGGGCACAAGAACGGCGGCAGCAATAGCGACCAGAGCGGGAGAAACCGCAGGCAGATGTCGTTGCTTTTTCCGTGGGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTgcggcg < 2:491054/280‑1 (MQ=255) tg‑tgGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGt > 2:138791/1‑280 (MQ=255) gttggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTGTTGGTGATAGTCCGTTGCCGGGCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACAGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCGGAGTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTa < 1:220972/277‑1 (MQ=255) ttggCCCACGCCCTCCCGGCTTGCGGCTTTCCATCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACa < 2:307443/279‑1 (MQ=255) tgGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTa < 1:554314/279‑1 (MQ=255) gggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCCGATTTCGTTGATTTTTCCGTGAGGGGCTCTTTTTTC‑GCCGCGACTGTGCCGGTTGGCTGCGGGGTACAt > 1:720335/1‑281 (MQ=255) gggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACAt > 1:669314/1‑281 (MQ=255) gcgcACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAACGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCTCTTTCTGGCTTTGCAGCGCCTCGCACTCTTCTCTCTTGTTATGCGTACTGATTTACAGATAACCGATAACGGTCACAAGAACGGCGGCCGTAATAGCGACTATAGCCGGAGAAACCGCAGGCAGCTTTCGTTGCTTTTTCCCTGTGGTGC‑CTTCTTTCAGCCGCCATGGTGCCCGCTGGGTGGCGCGTatccaa > 2:223998/1‑276 (MQ=255) gcacgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGCTGGCTGCGGCGTACATAAt > 1:918218/1‑280 (MQ=255) gcacacacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCACCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAAt > 1:795057/1‑280 (MQ=255) cacgcacTCCGCTCTGGCGACTTGCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTTGTAGTCCGTTTCCTGTCACTGTCTGGCTTTGCAGCGTCTCTGTCTCTTCTTTCTTGTGATGCGTAATGAAGTACCGACCACCGATAAAGGTCACAGGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAAtct < 2:667275/281‑1 (MQ=255) cTCCCGGCTTGCGCTTTTCCAGCCCTTTATTGT‑AGGTCCGGTGTTCTTCTGGTTTTTGTGTTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGATTCTTCTTTCTTGTGATGCATAATAAAGTACAGACCACCGATAAAGGTCACAAGAACAGCGGCAGCAATAGCGACCATAGCGGGAGAGGCCGCAGGCAGATTTCGTTGCTTTTTCCGTAAGGTGCTCGTTTTTC‑GCAGCGAAGGTGCCGGTTGACTGCGGCGTACATAATCTCGTtg < 2:640336/280‑1 (MQ=255) cTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTtg < 1:214678/280‑1 (MQ=255) ccGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGTGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCCGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTCGGCTGCGGCCTACATacttctgttttgcc > 1:505972/1‑268 (MQ=255) ggCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTg < 1:727379/251‑1 (MQ=255) ggCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTg > 2:727379/1‑251 (MQ=255) ggCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATACCGGGAGAAACCGCAGGCAGATTTCGTTTCTTTTTCCGTGGGGTGCTCTTTTTTC‑GCCGCCAAGGTTCCGGTTGGCTGGGGCTGTCCTTATCTCGTTGTGCCACTTTc > 2:549449/1‑281 (MQ=255) aCTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCCGCGTCTCGGACTCTTCTTGCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTGAGTTGCTTCTTCCGTGCGCTGCTCTTTTTTA‑GCGGCGCAGGTGCCGGTTGGCTGCGGCGTACATAATCTCTTGGTGCCCCTATCGttt > 2:774565/1‑281 (MQ=255) aCTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGTGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGttt > 1:1035670/1‑281 (MQ=255) ttCCATCTCTTTAATTT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCg < 1:371393/280‑1 (MQ=255) ttCCAGCTCTTTAATGT‑ATCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCg < 1:1009329/280‑1 (MQ=255) ttCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCg < 1:549449/280‑1 (MQ=255) tCCGGCTCTTTCATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCt < 1:718672/281‑1 (MQ=255) tCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACGATCGTTTCGCt > 1:986754/1‑281 (MQ=255) tAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTAttc > 1:563659/1‑281 (MQ=255) cgcCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCa > 1:249578/1‑281 (MQ=255) cgcCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCGGTATTTATTCGTTCGTCa > 2:1039728/1‑281 (MQ=255) aGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATATCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTTAGccc > 1:921453/1‑281 (MQ=255) cttctGTTTTTGGTGTTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGGGGTGCTCTTTTGTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCAt < 1:1056495/280‑1 (MQ=255) ttttGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTa < 1:138791/281‑1 (MQ=255) ggtggtAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGTCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGGCGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTGGCGGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAgcg > 1:739029/1‑281 (MQ=255) ggtggtAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCCCTTTTTTC‑GCCGCGAGGGTGCCGGTTGGCTGCGGCGTACATCATCTCGTTGTGCCACTATTGGTTCTCGGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAgcg > 2:826108/1‑281 (MQ=255) gtAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGCTGCGTAATTAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAgcggcg < 1:774565/280‑1 (MQ=255) aGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAACGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGc > 1:420745/1‑281 (MQ=255) tttCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGTGGCGGGCCTTTGa > 1:945445/1‑281 (MQ=255) tttCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTCCCGGTTGGCTGCGGCGTACATCATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCCGCATGTTACTTAAGCGGCGGTCCTTTg > 2:327263/1‑280 (MQ=255) ttCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGTGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGa < 2:1035670/280‑1 (MQ=255) ttCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGa < 2:669314/280‑1 (MQ=255) cACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGc > 2:155491/1‑207 (MQ=255) cACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGc < 1:155491/207‑1 (MQ=255) tttggtgttttGAAGCCGCTCTGCCTCTTCTTTGTTGTGATGCGTAATGAAGTCACGACAACCGATAAAGGTCACAAGGACGGCGGCAGCAATATCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTGTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGc < 2:720335/273‑1 (MQ=255) tttCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTTATGCATACTGAAGTACAGACCACCGATAAAGGTCACAGGACCGGCGGCAGCAATAGCGACCATAGCGGGAGAACCCGCAGGCAGATTTCGTTGCTTTTTCCCTGAGGTACTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGc < 1:167169/281‑1 (MQ=255) tccTGGCTTTACGCCGTATCAGACTGTTCTTTGTTGTGATGCGTGGTGAACTACACACCCCCGAAAAGGGTCACAAGAGCGGCGGCAACAATTGGGACCATGGCGGGAGAAACCGACGGCAGATTTCGTGGCTTTTTCCGTGGGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGTCCAGTCACGGCa < 2:326681/279‑1 (MQ=255) cTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTa < 1:1051714/281‑1 (MQ=255) tGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GTCGCGAAGGCGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGGATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCAGGGCAGTCCTAAAg > 1:404420/1‑281 (MQ=255) cAGCGTGTCGGTCTCTTCTTTCTTGTGATCCTTAATGAAGTACAGACAACCGATACAGGGCACAAGTACGGCGGCATCAATAGCGACCATCGCGGGAGAAACCGCAGCCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCGACTATCGTTTCGCTGTAGTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGt < 2:739029/281‑1 (MQ=255) aGCGTCTCGGACTCTTCTTTATTGTGGTGCGTAATGAAGTGCAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGtt < 1:223998/281‑1 (MQ=255) aGCGTCTCCGACTCTTCTTTCTTGTGATGCGTAATGAAGTCCATACCACCCATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGtt < 2:986754/281‑1 (MQ=255) agctctcGGACTCTTCTTTCTTGGGATGCGTAATGAACGACAGACCACCAAAAAAGGTAACAAGAACGACGGCAGCAATAGCGACCATGGCGGGGGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGGGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGtt < 2:483137/277‑1 (MQ=255) tcGGCCCCTTCTTTCTGTGGTTGCTTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACttt < 2:360071/281‑1 (MQ=255) attttctttctGGTGGTGGGTAATGGAGTAACAACCGCCGATCAAGGTCACAAGAACGGCCGCAGCAAAAGCGACCATAACGGGAGAAACCGCAGGATGATTTGGTTTGTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGGGTACAAAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGt < 2:945445/278‑1 (MQ=255) tcttGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCGCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGTAGTCTTAAAGGTTTACCTTCAGGTAAcgcgc > 1:446003/1‑280 (MQ=255) tgtgATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCCCGTTGTGCCACTATCGTGTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCgtgt > 1:487192/1‑281 (MQ=255) tgATGTGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGa > 2:143941/1‑233 (MQ=255) tgATGTGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGa < 1:143941/233‑1 (MQ=255) tgATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGtt < 1:821016/257‑1 (MQ=255) tgATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGtt > 2:821016/1‑257 (MQ=255) cGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGGGAAGGTGCCGGTTGGCTGGGGCGTACATAATCTCGTTGTGCCCCTATCGGTTCGCTGTATTTATTCGTTCGTCAGCCCCCACTGTTACTTAAGCGGCGGGCCTTTGACCAGGCACGGCAGTCTTAAAGGGTTAATTTAAGGGAAcgggggggtttgaccc > 2:564214/1‑267 (MQ=255) cGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTCTTTATTCGGTCGTCAGCCCGCCATGTTCCTTAAGCGTCTGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTCACTTTAATGTAACGTGCGTGTTG‑Tcccc > 1:627226/1‑281 (MQ=255) cGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATccc < 1:1039728/281‑1 (MQ=255) cGTAAGGAAGTCCCGACCCCCGATAAAGTTCACAAGAACGGCGGCAGCAATCGCGACCGTAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATccc < 2:563659/281‑1 (MQ=255) | CTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCC > NC_000913/4122896‑4123456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |