New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1828864 =	143 (0.860)	8 (0.050)	8/444	12.1	5.4%	coding (871/1479 nt)	astD	succinylglutamic semialdehyde dehydrogenase
	?	NC_000913	1828903 =	143 (0.900)					coding (832/1479 nt)	astD	succinylglutamic semialdehyde dehydrogenase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGCTGAACCTGGTGCAGGGCGGGCGTGAAACGGGTCAGGCGCTGAGTGCGCTGGAGGATCTCGACGGTTTGCTGTTTACCGGTAGCGCCAATACAGGCTACCAGTTGCATCGCCAGCTCTCCGGTCAGCCGGAGAAAATTCTCGCCCTTGAGATGGGCGGTAATAATCCGCTAATTATCGATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAAGCGGGGCGCAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1829157‑1828864 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggcgcaggTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCAT  >  NC_000913/1828903‑1829186                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    TGCTGAACCTGGTGCAGGGCGGGCGTGAAACGGGTCAGGCGCTGAGTGCGCTGGAGGATCTCGACGGTTTGCTGTTTACCGGTAGCGCCAATACAGGCTACCAGTTGCATCGCCAGCTCTCCGGTCAGCCGGAGAAAATTCTCGCCCTTGAGATGGGCGGTAATAATCCGCTAATTATCGATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAAGCGGGGC                                                                                                                                                                                                                                                                                                   >  1:739770/1‑289                                                                      TGCTGTTTACCGGTAGCGCCAATACAGGCTACCAGTTGCATCGCCAGCTCTCCGGTCAGCCGGAGAAAATTCTCGCCCTTGAGATGGGCGGTAATAATCCGCTAATTATCGATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACTTGCGCCCGCCGTTTATTGCTGAAAAGCGGGGCGC                                                                                                                                                                                                                                                                                                 >  2:442979/1‑222                                                                      TGCTGTTTACCGGTAGCGCCAATACAGGCTACCAGTTGCATCGCCAGCTCTCCGGTCAGCCGGAGAAAATTCTCGCCCTTGAGATGGGCGGTAATAATCCGCTAATTATCGATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAAGCGGGGCGC                                                                                                                                                                                                                                                                                                 <  1:442979/222‑1                                                                                                           GCATCGCCAGCTCTCCGGTCAGCCGGAGAAAATTCTCGCCCTTGAGATGGGCGGTAATAATCCGCTAATTATCGATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAAGCGG                                                                                                                                                                                                                                                                                                      >  2:540900/1‑180                                                                                                           GCATCGCCAGCTCTCCGGTCAGCCGGAGAAAATTCTCGCCCTTGAGATGGGCGGTAATAATCCGCTAATTATCGATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAAGCGG                                                                                                                                                                                                                                                                                                      <  1:540900/180‑1                                                                                                                                            TCTCGCCCGTGGGATGGGCGGTAATAATCCGCTAATTATCGGTGTGGTGTCGGCTGTCGACGCGGCTGTCCGTCTGGCCATTCAGTCGTCTTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGACGTTTATTGCTGAAAAGCGGGGCGCAAGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATG                                                                                                                                                                                                                                                        <  2:733262/193‑1                                                                                                                                                            GGCGGTAATAATCCGCTAATTATCGATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAAGCGGGGCGCAGGTG                                                                                                                                                                                                                                                                                            <  1:624422/141‑1                                                                                                                                                            GGCGGTAATAATCCGCTAATTATCGATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAAGCGGGGCGCAGGTG                                                                                                                                                                                                                                                                                            >  2:624422/1‑141                                                                                                                                                            GGCGGTAATAATCCGCTAATTATCGATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAAGCGGGGCG                                                                                                                                                                                                                                                                                                  <  1:238676/135‑1                                                                                                                                                            GGCGGTAATAATCCGCTAATTATCGATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAAGCGGGGCG                                                                                                                                                                                                                                                                                                  >  2:238676/1‑135                                                                                                                                                                       TCCGCTAATTATCGATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGATTGTCACAGCCGGACAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAACCGGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCTACTGAATGGTCAG                                                                                                                                                                                                                                                   >  1:1340634/1‑171                                                                                                                                                                           CTAATTATCGATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAAGCGGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCC                                                                                                                                                                                                                                                                 >  2:1042084/1‑153                                                                                                                                                                           CTAATTATCGATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAAGCGGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCC                                                                                                                                                                                                                                                                 <  1:1042084/153‑1                                                                                                                                                                                     ATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAAGCGGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGA                                                                                                                                                                                                                                                           <  1:1595121/149‑1                                                                                                                                                                                     ATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAAGCGGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGA                                                                                                                                                                                                                                                           >  2:1595121/1‑149                                                                                                                                                                                                                                                                                                 CGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCA   >  1:1344938/1‑289                                                                                                                                                                                                                                                                                                   cgGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCAT  <  1:1040963/286‑1                                                                                                                                                                                                                                                                                                   CAGGTGCAGCTTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTGTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCAT  <  1:1508900/288‑1                                                                                                                                                                                                                                                                                                   CAGGGGCAGCGTTGACCGGCCGGGACAAACGCCGACTGAATGGTCAGATGGAAAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCAT  <  2:435115/288‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    TGCTGAACCTGGTGCAGGGCGGGCGTGAAACGGGTCAGGCGCTGAGTGCGCTGGAGGATCTCGACGGTTTGCTGTTTACCGGTAGCGCCAATACAGGCTACCAGTTGCATCGCCAGCTCTCCGGTCAGCCGGAGAAAATTCTCGCCCTTGAGATGGGCGGTAATAATCCGCTAATTATCGATGAGGTGGCGGATATCGACGCGGCTGTCCATCTGACCATTCAGTCGGCGTTTGTCACAGCCGGTCAACGCTGCACCTGCGCCCGCCGTTTATTGCTGAAAAGCGGGGCGCAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1829157‑1828864 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggcgcaggTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCAT  >  NC_000913/1828903‑1829186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.