Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,809,552 T→G intergenic (+59/‑65) ygaY → / → ygaZ pseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein/putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,809,5520TG98.3% 217.9 / NA 59intergenic (+59/‑65)ygaY/ygaZpseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein/putative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (27/31);  minor base C (1/0);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTAACCAGACGGTAATTTATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGG  >  NC_000913/2809269‑2809834
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                          
aCTAACCAGACGGTAATTTATCGGATACATCCTGATTCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGAt                                                                                                                                                                                                                                                                                       <  1:1190756/289‑1 (MQ=255)
    aCCAGACGGTAATTTATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCATCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGCTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATCAGGGTGTTAAGAAGCCCATCAGGCTGATAAgg                                                                                                                                                                                                                                                                                   >  1:648219/1‑289 (MQ=255)
          cGGTAATTTTTGGGTTACATCCTGATGCGCGCGATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGGGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTaa                                                                                                                                                                                                                                                                               <  2:1217988/287‑1 (MQ=255)
          cGGTAATTTATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTCCTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTaa                                                                                                                                                                                                                                                                               <  2:1361886/287‑1 (MQ=255)
          cGGTAATTTATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGTGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTaa                                                                                                                                                                                                                                                                               <  1:663483/287‑1 (MQ=255)
                       gttACATCCTGATGCGCGGAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTCTTGGCGGTGCGGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGt                                                                                                                                                                                                                                                                 <  1:725389/286‑1 (MQ=255)
                       ggtaaaTCCTGATGCCGGAAATCGCCTGACCGCAGGGTACTTGACTGGCTCCTTGATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGt                                                                                                                                                                                                                                                                 <  2:859864/283‑1 (MQ=255)
                             tCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCGCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATattcattc                                                                                                                                                                                                                                                                    <  1:1740402/279‑1 (MQ=255)
                                 gATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACCTTATTGGCGGTGCCGCCGGTTCGCTAATTTAAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGCTTGCCCTGGTTAACTTACTGGTCTGGTGGCGGGGTTTTAATCGCCAGGGAGCCGCAAATTAATCATTCAGCAACAGTTTTAGTGGCTTTACACTTATAAGCGTGTTAAGAAGCCGATCAGGCAGGTACGGTTAAGTACTACATTCAGGCTATTTataat                                                                                                                                                                                                                                                      >  2:725389/1‑289 (MQ=255)
                                  aTGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTataata                                                                                                                                                                                                                                                     >  1:626875/1‑289 (MQ=255)
                                    gcgcgaAATCGCCTGACCGAAGGTTACATTACTAGCTACGTTATTGGGGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGGCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATAtt                                                                                                                                                                                                                                                   <  2:626875/289‑1 (MQ=255)
                                       cgcAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGTCTGGCGTGACGATTGCCCTGGTTCACTTACTGGCCTGGAGGAGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGCTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCAATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTataa                                                                                                                                                                                                                                                       >  1:362201/1‑282 (MQ=255)
                                       cgcAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAAc                                                                                                                                                                                                                                                >  1:588758/1‑289 (MQ=255)
                                       cgcAATCGCCTGACCGCAGGTTACATGACTAGCCACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTACCTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATAAGCAACCGTTTTCGTGGCTTTTCACTTATAAGGGTTTTAAGAAGCCCATAAGCCTTATAAGGTTAAACAATTCATTACGTCTAtttttttttccctt                                                                                                                                                                                                                                                >  2:1740402/1‑278 (MQ=255)
                                              gCCTGACCGCAGGTTAGATGACTGTATACTTGTTTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACTTGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGTTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGtt                                                                                                                                                                                                                                         <  2:737185/289‑1 (MQ=255)
                                               ccTGACCGCAGGTTACATGACTGGCTACTGTATTGGCGGGTCCGCCGGTTCGCTAATTTCAGCCCGCGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTa                                                                                                                                                                                                                                        <  2:863994/289‑1 (MQ=255)
                                                  gACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGAt                                                                                                                                                                                                                                                                                       <  2:529451/239‑1 (MQ=255)
                                                  gACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGAt                                                                                                                                                                                                                                                                                       >  1:529451/1‑239 (MQ=255)
                                                             aCATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACAATCTAt                                                                                                                                                                                                                          >  1:875048/1‑289 (MQ=255)
                                                                 gACTAGCTACTTTTTTGGCGGGGCGGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTTGCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTGTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAATGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGa                                                                                                                                                                                                                       <  2:648219/288‑1 (MQ=255)
                                                                     aGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGcc                                                                                                                                                                                                                  <  2:588758/289‑1 (MQ=255)
                                                                      gCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTTGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATattcatt                                                                                                                                                                                                                                                                     >  1:566151/1‑237 (MQ=255)
                                                                                    ggTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCTCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCTGCTAATTACGCAATCAGCCACCGTTTTCGTGGCTTTCCCTTTATAAGTGCGTTAATAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAACGTACACTCTATGAAAAGCCCTACACCACACCCt                                                                                                                                                                                                    >  2:384470/1‑288 (MQ=255)
                                                                                     gTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGata                                                                                                                                                                                                                                                                           <  1:717396/216‑1 (MQ=255)
                                                                                     gTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGata                                                                                                                                                                                                                                                                           >  2:717396/1‑216 (MQ=255)
                                                                                     gTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCt                                                                                                                                                                                                    >  1:1574381/1‑287 (MQ=255)
                                                                                       gccgccGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCt                                                                                                                                                                                                 >  2:1706890/1‑288 (MQ=255)
                                                                                               ttagCTAATTTCAGCCTCGGCCTGGAAACGTGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCg                                                                                                                                                                                        <  2:875048/286‑1 (MQ=255)
                                                                                                    tAATGTAAGCCTCAGCCTGCCGTGTTGGCGGTTCGGCTGTGGGTTGACTGGCTGGCGTGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAATCCGCAAATTAATCAATCAGCAACCGTTTTCGTTGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGAc                                                                                                                                                                                   <  2:166814/289‑1 (MQ=255)
                                                                                                               tcagcctGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAg                                                                                                                                                                        <  2:1574381/289‑1 (MQ=255)
                                                                                                                             tGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTa                                                                                                                                                                                                                                  >  2:1046308/1‑217 (MQ=255)
                                                                                                                             tGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTa                                                                                                                                                                                                                                  <  1:1046308/217‑1 (MQ=255)
                                                                                                                                            cGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGttat                                                                                                                                            <  1:1706890/288‑1 (MQ=255)
                                                                                                                                                          gcgcGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAgg                                                                                                                                                                                                                                                                                   <  1:1036940/139‑1 (MQ=255)
                                                                                                                                                          gcgcGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAgg                                                                                                                                                                                                                                                                                   >  2:1036940/1‑139 (MQ=255)
                                                                                                                                                                cgaTTGCCCTGGTTAACTTATTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACAGGTTTCGTGGCTGTAAACTTATAAGGCTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAACGCCCTACTCCCCAGCCTGCTCCTGGTTCGTCCACCTTCATGGAAGGCTGCCAAGACAGTTTACCGATTGTTTTTAGTTATATTCCGGTCGCCtt                                                                                                                       >  2:1571293/1‑289 (MQ=255)
                                                                                                                                                                       ccTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCGATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGtt                                                                                                                 <  1:1571293/288‑1 (MQ=255)
                                                                                                                                                                                    ctggtctggtGGCGTGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTAGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCATTTGCGTTCGGTCTGAATGCGa                                                                                                   <  1:384470/289‑1 (MQ=255)
                                                                                                                                                                                     tggtctggtGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATAtt                                                                                                                                                                                                                                                   <  2:1136835/144‑1 (MQ=255)
                                                                                                                                                                                     tggtctggtGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATAtt                                                                                                                                                                                                                                                   >  1:1136835/1‑144 (MQ=255)
                                                                                                                                                                                                 gAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGAt                                                                                                                                                                                                                                                                                       >  2:1366966/1‑96 (MQ=255)
                                                                                                                                                                                                 gAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGAt                                                                                                                                                                                                                                                                                       <  1:1366966/96‑1 (MQ=255)
                                                                                                                                                                                                  aGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTac                                                                                                                                                                                                                                 <  2:1752994/149‑1 (MQ=255)
                                                                                                                                                                                                  aGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTac                                                                                                                                                                                                                                 >  1:1752994/1‑149 (MQ=255)
                                                                                                                                                                                                              tCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTaa                                                                                                                                                                                                                                                 >  2:1043300/1‑121 (MQ=255)
                                                                                                                                                                                                              tCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTaa                                                                                                                                                                                                                                                 <  1:1043300/121‑1 (MQ=255)
                                                                                                                                                                                                               cAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGAt                                                                                                                                                                                                                                                                                       >  1:138549/1‑82 (MQ=255)
                                                                                                                                                                                                               cAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGAt                                                                                                                                                                                                                                                                                       <  2:138549/82‑1 (MQ=255)
                                                                                                                                                                                                               cAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGt                                                                                                                                                                                                                                                                 <  2:375009/104‑1 (MQ=255)
                                                                                                                                                                                                               cAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGt                                                                                                                                                                                                                                                                 >  1:375009/1‑104 (MQ=255)
                                                                                                                                                                                                                                      cAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTac                                                                                                                                                                                                                                 <  2:1646427/113‑1 (MQ=255)
                                                                                                                                                                                                                                      cAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTac                                                                                                                                                                                                                                 >  1:1646427/1‑113 (MQ=255)
                                                                                                                                                                                                                                                                  ataAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGttat                                                                                                                                            <  1:592623/170‑1 (MQ=255)
                                                                                                                                                                                                                                                                  ataAGGGTGTTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGttat                                                                                                                                            >  2:592623/1‑170 (MQ=255)
                                                                                                                                                                                                                                                                          gtTAAGAAGCCCATCAGGCTGATAAGGTTAAGTTATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCg                                                                                                                                                    >  2:1344784/1‑154 (MQ=255)
                                                                                                                                                                                                                                                                          gtTAAGAAGCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCg                                                                                                                                                    <  1:1344784/154‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  gCCCATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAg      >  2:1719475/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                     cATCAGGCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCgg  >  2:1209264/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                     cATCAGGCTGATAAGGTTAAGATATTAATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATCCAAACACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCCCGAAAGCGTTTTTTTCTCCTCCATCCTTTATGCAGGCTCGCGCCCGTTCGTCATCACCGCGACTCTCGCAGCCgg  >  2:1228567/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                          
ACTAACCAGACGGTAATTTATCGGATACATCCTGATGCGCGCAATCGCCTGACCGCAGGTTACATGACTAGCTACTTTATTGGCGGTGCCGCCGGTTCGCTAATTTCAGCCTCAGCCTGGCAACATGGCGGTTGGGCTGGCGTTTGTCTGGCTGGCGCGACGATTGCCCTGGTTAACTTACTGGTCTGGTGGCGAGGTTTTCATCGTCAGGAAGCCGCAAATTAAGCAATCAGCAACCGTTTTCGTGGCTTTACACTTATAAGGGTGTTAAGAAGCCCATCAGTCTGATAAGGTTAAGATATTCATTCAGTCTATTTATAATATTAACAATCGTTAAGCGTACACTCTATGGAAAGCCCTACTCCACAGCCTGCTCCTGGTTCGGCGACCTTCATGGAAGGATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGG  >  NC_000913/2809269‑2809834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: