Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,184,809 G→T 82.8% G1189C (GGT→TGT)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,8090GT82.8% 302.7 / 61.4 148G1189C (GGT→TGT) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (12/14);  new base T (52/69);  total (65/83)
Fisher's exact test for biased strand distribution p-value = 8.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 151 total aligned reads displayed.

CGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGC  >  NC_000913/4184532‑4185076
                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                            
cGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACtgtg                                                                                                                                                                                                                                                                           >  1:354239/1‑281 (MQ=255)
cGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGGTCGACtgtg                                                                                                                                                                                                                                                                           >  1:1133707/1‑281 (MQ=255)
cGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGAAACGCCGGTGTTCGACtgtg                                                                                                                                                                                                                                                                           >  1:261398/1‑281 (MQ=255)
  tACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGATCCTCGAAACCCACCTTGGTATGGCTGCGAAAGCTATCGGCGACAAGATCAACGCCATGCTGAAACAGCACCAAGAAGTAGCTAAACTCAGCGACTCCATCCAGAGTGCGTACGCGCGGGGAGCCGACGTTCGACAGAAAGTCGACCTGAGTATCCTCAGCGATGAATAACGTATCCGTCTGGCTGAACACTTGATAAAAGCTATGCCAACCGCAACGCCGTTGCTCGACCATGCg                                                                                                                                                                                                                                                                         >  2:662015/1‑281 (MQ=255)
   aCTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATTGCAACGCCGGTGTGCGACGGTTCGa                                                                                                                                                                                                                                                                        >  1:977041/1‑281 (MQ=255)
     tGAACCCGCTGGGCGTACCGTCTCGTATGAAAAACGGTCAGATCCGCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGaa                                                                                                                                                                                                                                                                       <  2:450451/280‑1 (MQ=255)
           cGCTGGGCGTCCCGTCTCGAATGAACATCGGTCGAATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACTATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTAAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGc                                                                                                                                                                                                                                                                 <  2:397262/280‑1 (MQ=255)
            gCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAACGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAg                                                                                                                                                                                                                                                               >  1:363906/1‑281 (MQ=255)
                        ggctctgaTGAACACCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAagag                                                                                                                                                                                                                                                    <  2:786/273‑1 (MQ=255)
                        gTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAagag                                                                                                                                                                                                                                                    <  1:1086913/280‑1 (MQ=255)
                                     aTCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTg                                                                                                                                                                                                                                       >  1:289448/1‑280 (MQ=255)
                                        ggTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGAGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGCAGAAGTTATGCGTCTGGCCGAAAACCTGCGCAAACGTATGCCAACCGCAACGCCGGGGTTCGACTGTGCGAACGAAGGCGAAATTAAAGAGCCGCTGAAACTTGGcc                                                                                                                                                                                                                                    >  2:813575/1‑279 (MQ=255)
                                         gTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCg                                                                                                                                                                                                                                    >  1:421615/1‑279 (MQ=255)
                                              aTCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGGGCTGCAGAAAGTTGGCCACCTGcc                                                                                                                                                                                                                             >  2:295481/1‑281 (MQ=255)
                                                   cGAAACCCACCTGGGGAGGGCGGCGAAAGGTATCGGCGAAAAGATCAACGCCATGCTGAAACAGCAGCCAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACtt                                                                                                                                                                                                                        <  2:261398/281‑1 (MQ=255)
                                                    gAAACCCACCGGGGGATGGCTGCAAAAGGTGGAGGCGACAAGATCAAGCCCAAGCTGAAACAGCAGGAAGGAGTCGCGAAACTGCGCGAATTCATCCAGCTTGCGTACGATCTGGGCGCTGACGTGCGTCAGAAAGTTGACCTGGGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACtt                                                                                                                                                                                                                        <  2:977041/280‑1 (MQ=255)
                                                       aCCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCgg                                                                                                                                                                                                                    <  1:295186/281‑1 (MQ=255)
                                                           aCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAACGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGGGTTCGACTGGGCGAAAGAAGCAGAAATTAAAGAGCTGCGGAAATTTGGCGACCTGCCGACTTACGGt                                                                                                                                                                                                                   >  2:685793/1‑278 (MQ=255)
                                                           aCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGt                                                                                                                                                                                                                   >  2:725731/1‑278 (MQ=255)
                                                            ccTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGa                                                                                                                                                                                                               <  1:132147/281‑1 (MQ=255)
                                                            ccTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGa                                                                                                                                                                                                               <  1:1065589/281‑1 (MQ=255)
                                                                ggTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACtt                                                                                                                                                                                                                        >  1:852356/1‑268 (MQ=255)
                                                                ggTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACtt                                                                                                                                                                                                                        <  2:852356/268‑1 (MQ=255)
                                                                 ggaaggCTGAAAAAGGTATCACGGACAAGCTCAACGCCATGCTTGAACCGCGGACAGAAGTCGCGAAACTGCGCGAATTCATCCAGCTTGGGTCCGCTCTGTGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGATAACCTGCACAAAGGTATGCCAATCGCAACGCGGATGTTCGTCGGTGCGAAGGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCAGCTTCCGGTCAGATCCGc                                                                                                                                                                                                          <  2:723893/277‑1 (MQ=255)
                                                                  tATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCTTGCGTACGATCTGGGCGCTTACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTATGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACCTCAGGTCAGATCCGcc                                                                                                                                                                                                         >  2:276009/1‑281 (MQ=255)
                                                                  aagcgacaggAAAGGTATCTCCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGAGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGGAGTTTTGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCATAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGGCTTCCGGTCAGATTCGcc                                                                                                                                                                                                         <  1:949970/272‑1 (MQ=255)
                                                                      gCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTa                                                                                                                                                                                                     >  1:1185396/1‑281 (MQ=255)
                                                                      gCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTa                                                                                                                                                                                                     >  1:696177/1‑281 (MQ=255)
                                                                        tGCGAAAGGTATCGACGACAAGATCAACGCCATGCTGAAACAGCAGCAAGGAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTAc                                                                                                                                                                                                    <  1:1174089/280‑1 (MQ=255)
                                                                          cGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGAGACCTGACGACTTCGGGTCAGATCCGCGTGTACGAt                                                                                                                                                                                                 >  1:105100/1‑281 (MQ=255)
                                                                          cGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGAATGTGCGAACGAAGCAGAAATAAAAGAGCTGCTGAAACTTGGCGACTCGAGGACTTCCGGTCAGATCCGCCTGTACGa                                                                                                                                                                                                  >  2:309225/1‑280 (MQ=255)
                                                                           gAAAGGATTAGGCGAAAAGATAAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAGTGCGCGAATTCAGCCAGCGTGCGTACGATCTGGGCGCTGAAGTTGGGAAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCGACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGAt                                                                                                                                                                                                 <  2:1198773/280‑1 (MQ=255)
                                                                              aGGTATCGGCGAAAAGATCAACGACATGCTGAAGCAGCGACAAGAATTCGCGAAACTGCGCGAATTCATCCGGCGTGCGTATGATCTGGGCGGTGACGTTCGTCAGAAAGTTGACCTGAGTACATTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGAATGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGt                                                                                                                                                                                              <  1:856831/280‑1 (MQ=255)
                                                                               ggTATCGGGCACAAGGTCAACGACATGCTGAAACCACAGGAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCTTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGGTGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGTTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTc                                                                                                                                                                                             <  2:543779/280‑1 (MQ=255)
                                                                                gTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCg                                                                                                                                                                                            <  1:1034284/280‑1 (MQ=255)
                                                                                    cGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGTGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGcc                                                                                                                                                                                                                             >  2:332458/1‑243 (MQ=255)
                                                                                        gACAAGATCAAGGGCATGCTGAAACAGCAGCAAGAAGTCGTGAAACTTCGCGAATTCATTCATGTTGCATACGATCGGGGCGCGGACGTTCGTCAGAAATTTGTCCTGAGTAACTACAGCGATGAAGAAGTTGTGCGTCTGGCTGAAAACCTGCGCAAAGGTGTGCCAATCGCAACGCCGGTGGTCGACTGTGCGAAAGGAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGGCGACTTCCGGTCAGATCCGCCTGTACGAGGGTCGCACTGGTg                                                                                                                                                                                    <  2:823079/280‑1 (MQ=255)
                                                                                          caataccAACGGCATGCGCAAACAGCAGCAAGAATGCGCGAAACTGCTCGAATTCATCCAGCTGGCGTACGATCTGCGCGGTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCGGCGATGAAGAAGTTCTGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCTCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAAGTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGaa                                                                                                                                                                                  <  2:774579/274‑1 (MQ=255)
                                                                                          cAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCg                                                                                                                                                                                            <  1:476410/270‑1 (MQ=255)
                                                                                          cAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGaa                                                                                                                                                                                  <  2:1012653/280‑1 (MQ=255)
                                                                                           aaGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTACGGTCAGAATCGCCTGTACGATGGTCGCACTGGTGAACa                                                                                                                                                                                >  1:536964/1‑281 (MQ=255)
                                                                                                       aTGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACATGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTcc                                                                                                                                                                    >  1:1089004/1‑281 (MQ=255)
                                                                                                        tGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCg                                                                                                                                                                   >  1:1186717/1‑281 (MQ=255)
                                                                                                         gctagagcagcagGAAGAAGGCGCGAAACTTCGCGAGTTCATCCAGCGTGCGCACGAGTTTGGCGCGGACGTTCGTCAGAAAGTTAACCGTAGTACCTGCAGCGTGGAAGAAGGTATGCGTCTGGCTGAAAGCCTGCGAAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATGAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATGCGCCTGTACGGTGGTCGCACTGGTGAACAGTTCGAGCGTCCgg                                                                                                                                                                  <  2:192757/274‑1 (MQ=255)
                                                                                                          cTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGt                                                                                                                                                                 <  2:1186717/281‑1 (MQ=255)
                                                                                                                   cagcaAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGATGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGCGAACAGTTCGGTCGGCCGGTCACCGTTgg                                                                                                                                                       >  2:226734/1‑281 (MQ=255)
                                                                                                                   cagcaAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTTGTGAACAGTTCGGCCTTCCGGTAACCGTTgg                                                                                                                                                       >  2:621740/1‑281 (MQ=255)
                                                                                                                    agcaAGGAGTCGCGAAACTTCGCGAATTCATCCCGCGTGCGTACGATCTGGGGGGTGACGTTCGTGCGGAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCGTCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGGGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGt                                                                                                                                                      <  2:1041053/281‑1 (MQ=255)
                                                                                                                    agcaAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGt                                                                                                                                                      <  1:685793/281‑1 (MQ=255)
                                                                                                                    agcaAGAAGGCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGt                                                                                                                                                      <  2:696177/281‑1 (MQ=255)
                                                                                                                     gcaAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGt                                                                                                                                                      <  2:1185396/280‑1 (MQ=255)
                                                                                                                               gcgAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacatgta                                                                                                                                              <  2:354239/278‑1 (MQ=255)
                                                                                                                               gcgAAACTCCGCGAATTCATCCAGCCTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacatgta                                                                                                                                              <  1:309225/278‑1 (MQ=255)
                                                                                                                                                 ctcccaggtgcgtgcGAGTTGGGGGTTGACGGTCGTCAGAGAGTTGCCCTGAGTACCTTAAGCGATGAAGAAGTTAGGCGTCTGGCTAACAACCTGCGCAAAGGTATGCCAAGCGCAACGCAGGTGTGCGAATGGACCAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGGCCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAaccacc                                                                                                                          <  2:1133707/273‑1 (MQ=255)
                                                                                                                                                 aTCCAGCGTGCGTACGATCTGGGCGCTGACGGTCGTCAGATAGTTGACCTGAGTCCCTTCAGCGATGAAGAAGGTATTCGTCTGGCTGAAAACCTGCGCAGAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTGCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAaccacc                                                                                                                          <  2:376730/280‑1 (MQ=255)
                                                                                                                                                  tCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCt                                                                                                                         >  1:195784/1‑280 (MQ=255)
                                                                                                                                                      gcgtgcgtACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAGGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTcg                                                                                                                    <  1:323747/281‑1 (MQ=255)
                                                                                                                                                       cgtgcgtACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCg                                                                                                                                                                                            <  2:419868/209‑1 (MQ=255)
                                                                                                                                                       cgtgcgtACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCg                                                                                                                                                                                            >  1:419868/1‑209 (MQ=255)
                                                                                                                                                       cgtgcgtACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTcga                                                                                                                   >  2:1124623/1‑281 (MQ=255)
                                                                                                                                                       cgtgcgtACGATCTGGGCGCTGACGTGCGTCAGAAAGTTCACCTGAGTACCCTCAGCGATGAAAAAGTTATGCGTCTGGCTCAAAACCTGCGCAACGGTATGCCAATCGCAACGCCGGTGTTCGACGGCGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTGGGCGACCTTCCGCCTCCCGGTCAAGTCGGCCTGTAAGATGGCCGACCTGCAGAACAGTTCGACCGTCCGGTACACCGTTGGTACCGCACCCTGGTGTAACTGACCCACCTGGTcga                                                                                                                   >  2:1007648/1‑281 (MQ=255)
                                                                                                                                                         tgcgtACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacatgta                                                                                                                                              <  1:60184/252‑1 (MQ=255)
                                                                                                                                                           cgtACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGATGAAGTTATTCGTCTGGCTGAAAACCTGCACAAAGGTATGCCAACCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCATATCCGACTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTcgacga                                                                                                                >  1:432198/1‑280 (MQ=255)
                                                                                                                                                           cgtACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGAc                                                                                                                                                                                                                                  >  1:972616/1‑167 (MQ=255)
                                                                                                                                                           cgtACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGAc                                                                                                                                                                                                                                  <  2:972616/167‑1 (MQ=255)
                                                                                                                                                           cgtACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCa                                                                                                                                                                                          >  1:664726/1‑207 (MQ=255)
                                                                                                                                                           cgtACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCa                                                                                                                                                                                          <  2:664726/207‑1 (MQ=255)
                                                                                                                                                                      ggCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTTTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACTCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCAcg                                                                                                     <  2:420177/280‑1 (MQ=255)
                                                                                                                                                                      ggCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCAcg                                                                                                     >  2:948565/1‑280 (MQ=255)
                                                                                                                                                                          cTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGt                                                                                                >  1:435806/1‑281 (MQ=255)
                                                                                                                                                                            gACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacatgtaca                                                                                                                                            >  1:892254/1‑235 (MQ=255)
                                                                                                                                                                               gttctgcggacaGTTGTCCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGTTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCa                                                                                            <  1:793792/269‑1 (MQ=255)
                                                                                                                                                                               gTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCa                                                                                            <  1:1124623/280‑1 (MQ=255)
                                                                                                                                                                               gTTAGTAAGAAAGTTGACCTGAGTACCTTCAGCGGTGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGGGCGAAAGAAGCAGAAATTAAAGAGCTGGTGAAACTGGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCa                                                                                            <  2:589854/280‑1 (MQ=255)
                                                                                                                                                                                ttCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTg                                                                                                                                                        >  1:591340/1‑219 (MQ=255)
                                                                                                                                                                                ttCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTg                                                                                                                                                        <  2:591340/219‑1 (MQ=255)
                                                                                                                                                                                   gTCAGAAAGTTGACCTGAGTACCTTCGGGGATGAAGAAGTTATGCGTCTGGCTAAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCgg                                                                                        <  1:1017245/280‑1 (MQ=255)
                                                                                                                                                                                   gTCAGAAAGTTGACCTAAATACCTGCAGCGATGAAGAAGATATGCGGCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCgg                                                                                        <  1:867139/280‑1 (MQ=255)
                                                                                                                                                                                   gTCAAAAAGTTGACCTGAATACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAAGTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCgg                                                                                        <  1:512662/280‑1 (MQ=255)
                                                                                                                                                                                                    aGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTa                                                                       >  2:422211/1‑280 (MQ=255)
                                                                                                                                                                                                           tCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTg                                                                                                                                                                                    <  2:461675/165‑1 (MQ=255)
                                                                                                                                                                                                              gCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCg                                                            >  1:1178663/1‑281 (MQ=255)
                                                                                                                                                                                                                 ctGAATAAGTTAAGCGTCTGGCTGACAACCTGGCAAAAGTTTTGCCAATCGAAACGCCGGTGTTCGACTGTACGAAAAAAGAAACAAATAACAAACCGCTGAACCTTGACGACCTGCCGACTTCCGGTCAAGTCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTAGGTTGCATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTg                                                         <  2:48740/280‑1 (MQ=255)
                                                                                                                                                                                                                  tGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTgg                                                        >  1:56378/1‑281 (MQ=255)
                                                                                                                                                                                                                  tGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTggg                                                       <  1:213311/281‑1 (MQ=255)
                                                                                                                                                                                                                        agTTATGCGTCTGGCTGAAACCCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGCGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTa                                                  <  1:461813/281‑1 (MQ=255)
                                                                                                                                                                                                                          tttagcgttcggCTGAAAGCCTGCGCAAAGGTATGCCAATAGCAACCCCCGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGATGGTAAg                                                <  1:662015/271‑1 (MQ=255)
                                                                                                                                                                                                                            aTGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGaaa                                                                                                                                    >  2:1234149/1‑195 (MQ=255)
                                                                                                                                                                                                                            aTGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGaaa                                                                                                                                    <  1:1234149/195‑1 (MQ=255)
                                                                                                                                                                                                                             tgggTCTGGCTGAAAACCGGCGCAAAGGTATGCCAATCGCAACGCCGGTGGTCGACGGTGCGAAAGAAGCCGAAATTAAACAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCACACTGGTGAGCAGTTCGAGCTTCCGGTAACCGTTGGTTACATGTGCATGCTGAAACGGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGca                                             <  2:504094/278‑1 (MQ=255)
                                                                                                                                                                                                                                   tGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTg                                                                                                                                                        <  2:1069492/168‑1 (MQ=255)
                                                                                                                                                                                                                                    ggCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATc                                                                                                                                                                                                             <  2:643057/115‑1 (MQ=255)
                                                                                                                                                                                                                                    ggCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCg                                      <  1:256968/281‑1 (MQ=255)
                                                                                                                                                                                                                                           aaCCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTcagcag                                                               >  1:567491/1‑249 (MQ=255)
                                                                                                                                                                                                                                           aaCCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTcagcag                                                               <  2:567491/249‑1 (MQ=255)
                                                                                                                                                                                                                                                gcgcAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTTCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCCGTAACCGTTCGTTACATGTACATGCTTAAACTGAACCACCTGGTCGAGGACAAGATGCACGCGCGTTCCACCGGTTTTTAAAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCCCAGCGCGGTGGTCGGCGtt                          >  2:448045/1‑281 (MQ=255)
                                                                                                                                                                                                                                                      aGGTATGCCAATCGCAAGGCCGGTGTTGGACTGTCCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTTCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCgggg                    <  1:737401/281‑1 (MQ=255)
                                                                                                                                                                                                                                                           tGCCCATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATg               >  1:940629/1‑281 (MQ=255)
                                                                                                                                                                                                                                                                  cGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGATGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTTGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCgggg                    <  2:85193/269‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gCCGGTGTTCGACTGAGCTAAAGAAGCAGGAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGgcgc  <  1:935599/281‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                            
CGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTTCGGGGAGATGGAAGTGTGGGCGC  >  NC_000913/4184532‑4185076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: