Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,495,101 T→C 5.8% intergenic (‑30/‑187) ydcI ← / → ydcJ putative DNA‑binding transcriptional regulator/putative metalloenzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,495,1010TC 5.8% 225.8 / 2.6 69intergenic (‑30/‑187)ydcI/ydcJputative DNA‑binding transcriptional regulator/putative metalloenzyme
Reads supporting (aligned to +/- strand):  ref base T (35/30);  new base C (2/2);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

GCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGCCATGTACCAGCAAGAAGTTCCGCAATATGGCAC  >  NC_000913/1494821‑1495379
                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                      
gCGTCAAAAACTCCGACTCCATGCGTTAAAAATTGTTCCCCGGGTAAGTTAATTTGCGCCCCCTGACGACCACGCTCAAACAGCGAGCGCCAGTCAGCTGCTCCAGTTTATTCAATGTCTTAGAGAGCGCAGGTTGACGTAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTg                                                                                                                                                                                                                                                                                       <  2:1202335/281‑1 (MQ=255)
 cGTCAATAACTCTGCATGCATGCGTTACAAATTGTTCGCCGGGGAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGGTGGTCCAGTTCATTCAAAGTCTTAGAGGGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCt                                                                                                                                                                                                                                                                                        <  2:367773/280‑1 (MQ=255)
  gTCAAGAACTCTGACTGCATGTGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGa                                                                                                                                                                                                                                                                                      >  2:284753/1‑281 (MQ=255)
                   cctGCTTTAAAAATTGTTCGCCGGGTAATGTAAGTTGCGCCCCCTGACTACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCGCCAGTTCATTCCATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGa                                                                                                                                                                                                                                                                     <  2:369385/279‑1 (MQ=255)
                     tGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCCGACAAGTGAAGTTGTTTGa                                                                                                                                                                                                                                                                     >  1:1007212/1‑279 (MQ=255)
                     tGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCCGACAAGTGAAGTTGTTTGa                                                                                                                                                                                                                                                                     <  2:1007212/279‑1 (MQ=255)
                          tAAAAGTTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGAt                                                                                                                                                                                                                                                              <  1:903630/281‑1 (MQ=255)
                                  gTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGAt                                                                                                                                                                                                                                                      <  1:284753/281‑1 (MQ=255)
                                                gtacgTTTCGCCCCCTGACGACCACGCTCAAACCAGCGCGGGCCAGTCAGCTGCTCCATTTCATTCAATGTCTTAGGTGGCGCTGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATattatt                                                                                                                                                                                                                                        <  2:1221856/281‑1 (MQ=255)
                                                              cTGACGACCACGCTCAGACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCGTTCAATGTCTTAGAGTGCGCAAGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACGGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAATGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGc                                                                                                                                                                                                                            <  1:336960/279‑1 (MQ=255)
                                                               ttacgccCACGCTCAAACAAGCTAGCGCCAGTCACCTGCTCCAGTTCATTCAATTTCTTAGATAGCGCAGGTTGACTCAAATTAAGGGTGTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACTAATGTATGAAGGTGGCGCAAACGGATGCTCTGACTAAACAGTCTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCaa                                                                                                                                                                                                                          <  2:470213/278‑1 (MQ=255)
                                                                       aCGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAAtt                                                                                                                                                                                                                        >  1:470213/1‑274 (MQ=255)
                                                                        cGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCTCAAAGTTCCTTGTTGTGCGACAGCTACGCTTGTCTGAAGGTGGTGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGTTGTTAAAAACGAAGCGGTGTCGATATCAAGTGCCGTTGTTTGGTTATGAGAACTTGATTGCAAAATATTATTAACAATTAAAGCATTTATGTTACa                                                                                                                                                                                                                >  2:837052/1‑281 (MQ=255)
                                                                        cGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACa                                                                                                                                                                                                                >  1:426240/1‑281 (MQ=255)
                                                                                   gcgagcgCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGat                                                                                                                                                                                                     >  1:72077/1‑281 (MQ=255)
                                                                                                       ccAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCgcggc                                                                                                                                                                                 >  1:445224/1‑281 (MQ=255)
                                                                                                                    tGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATAttatta                                                                                                                                                                                                                                       <  2:1013835/214‑1 (MQ=255)
                                                                                                                    tGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATAttatta                                                                                                                                                                                                                                       >  1:1013835/1‑214 (MQ=255)
                                                                                                                              agCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAATGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAAttctt                                                                                                                                                          >  1:961683/1‑281 (MQ=255)
                                                                                                                              agCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACGATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTGGATTATGATAACTTGATTGCAAATTATTATTAACAATCAAAGCAATTATGTTACAGCAAAATGGATAATATTGAAGTTTTCGCGGCGAGAAAACAGTCTGTAAAttctt                                                                                                                                                          >  2:523331/1‑281 (MQ=255)
                                                                                                                                cgcAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTcc                                                                                                                                                        >  2:494760/1‑281 (MQ=255)
                                                                                                                                cgcAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTcc                                                                                                                                                        >  2:1020345/1‑281 (MQ=255)
                                                                                                                                cgcAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTAAAGCAAACTGGATAATATTGATGTTTTAGAGGCGAGATCAAAGTTTGTAAATTCTTcc                                                                                                                                                        >  2:775316/1‑281 (MQ=255)
                                                                                                                                cgcAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCCGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTcc                                                                                                                                                        >  1:1132497/1‑281 (MQ=255)
                                                                                                                                     gTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGc                                                                                                                                                     <  1:494760/279‑1 (MQ=255)
                                                                                                                                        gACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGAAAATATTGATGTTTTCGCGGCGAGATTAAAGTTTGTAAATTCTTCACGCAagag                                                                                                                                                >  1:505662/1‑281 (MQ=255)
                                                                                                                                               agtAAGGGTTTCAGCCGCGGGGCCCAAAGTTCCTTGTTGTGCGACAGCTACTAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCCGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATg                                                                                                                                          <  2:1132497/278‑1 (MQ=255)
                                                                                                                                                ttAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCg                                                                                                                                        <  2:72077/281‑1 (MQ=255)
                                                                                                                                                        ttCGGCCGCGCGCCCCAAAGTTCCTTGTTTGTGGACAGCTACGAATGTATGGAGTTGGCGCAAACGGATGCGCTGACTTAACAGACTATTTTTTTCCATAAGCTATGTTAAAAACGGAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTa                                                                                                                                <  2:505662/281‑1 (MQ=255)
                                                                                                                                                          cAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCTACCACTACGAATTTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGAGTTGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTaca                                                                                                                              <  1:1179604/281‑1 (MQ=255)
                                                                                                                                                          cAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAATCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTaca                                                                                                                              <  2:805947/281‑1 (MQ=255)
                                                                                                                                                                               cTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGCCTATTTTTTTCCCTACGCGATGTTCAAAACGAAGCGGTGTCGCTGACAAGTGACGTTGTTTGCTTATGATAACTTGATTGCAAAATATTATTAACAATTAACGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGCCCCCAGTTTGTAAATTCTACCCGCAAGAGTGAATCCTGTTACCTACACTCCATATTACTTCCCAcgg                                                                                                          >  2:210682/1‑278 (MQ=255)
                                                                                                                                                                                       tgCGACAGCTACGAATGTATGAATGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATTTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGa                                                                                                  <  2:961683/281‑1 (MQ=255)
                                                                                                                                                                                               cTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGAt                                                                                                                                                                                                                                                                    >  1:53681/1‑111 (MQ=255)
                                                                                                                                                                                               cTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGAt                                                                                                                                                                                                                                                                    <  2:53681/111‑1 (MQ=255)
                                                                                                                                                                                               cTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCACGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATgg                                                                                          >  1:501825/1‑281 (MQ=255)
                                                                                                                                                                                                         aTGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCAAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCAt                                                                                >  2:335442/1‑281 (MQ=255)
                                                                                                                                                                                                          tGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAg                                                                                                                                                                                                                                                                             <  1:248200/91‑1 (MQ=255)
                                                                                                                                                                                                          tGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAg                                                                                                                                                                                                                                                                             >  2:248200/1‑91 (MQ=255)
                                                                                                                                                                                                             aGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCAc                                                                             <  1:335442/280‑1 (MQ=255)
                                                                                                                                                                                                               gTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGaca                                                                                                <  1:200935/259‑1 (MQ=255)
                                                                                                                                                                                                                 ggCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGTAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTAATGCCCCCTGGCGGCAGAACCTATGGCGAAcctatctcttata                                                                         >  2:200935/1‑268 (MQ=255)
                                                                                                                                                                                                                  gcgcAAACGGATGTGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCa                                                                                                                         >  1:114032/1‑231 (MQ=255)
                                                                                                                                                                                                                  gcgcAAACGGATGTGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCa                                                                                                                         <  2:114032/231‑1 (MQ=255)
                                                                                                                                                                                                                  gcgcAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGa                                                                                                                                                                                                      <  2:1209088/154‑1 (MQ=255)
                                                                                                                                                                                                                  gcgcAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGa                                                                                                                                                                                                      >  1:1209088/1‑154 (MQ=255)
                                                                                                                                                                                                                      aaaCGGATGCGGTGGCTAAACAGACTAATTTTTTCCAGAAGCGATGTTAAAAACGAAGCGGGGTCGCTGACAAGGGAAGTTGTTTGATTATGATAACTTGGTTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATga                                                                    <  2:426240/280‑1 (MQ=255)
                                                                                                                                                                                                                            aTGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCgg                                                             >  1:501982/1‑281 (MQ=255)
                                                                                                                                                                                                                                      tAAACAGACGATTGTTTTCCATAAGCGATGTTAAAAACTAAGCGGTTTCGCTGACAAGTGAAGTTGTTTGTGTATGATAACTTGATTGCGAAATATTATTAACAATTAAAGCAATTATTGTACAGCAAGATGGATAATATTGATGTTTTCACGGCTAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTTGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGtttt                                                   <  2:501825/281‑1 (MQ=255)
                                                                                                                                                                                                                                             aCTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAgg                                             <  1:1020345/280‑1 (MQ=255)
                                                                                                                                                                                                                                              cTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGATGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGATCACTGGAGGCAGACATTATGGCGAACAGCATTACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGc                                            >  1:491873/1‑280 (MQ=255)
                                                                                                                                                                                                                                              cTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGa                                                                                                                                                                                                                                                                     <  2:246423/63‑1 (MQ=255)
                                                                                                                                                                                                                                              cTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGa                                                                                                                                                                                                                                                                     >  1:246423/1‑63 (MQ=255)
                                                                                                                                                                                                                                              cTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGc                                            >  1:171979/1‑280 (MQ=255)
                                                                                                                                                                                                                                               tATTTTTTTCCATAAGCGATGTTAAAAAAGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAAATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTgg                                                                                                         >  2:289560/1‑218 (MQ=255)
                                                                                                                                                                                                                                               tATTTTTTTCCATAAGCGATGTTAAAAAAGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAAATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTgg                                                                                                         <  1:289560/218‑1 (MQ=255)
                                                                                                                                                                                                                                                  ttttttCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCACGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATAACGGCGGAGGAGATTCGGGAACAGTTTTCGTAGGccaggt                                       >  1:352001/1‑276 (MQ=255)
                                                                                                                                                                                                                                                      ttCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGGAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAg                                    <  2:445224/280‑1 (MQ=255)
                                                                                                                                                                                                                                                       tCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGACCAGCATCACGGCCGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGcc                                  >  1:936543/1‑281 (MQ=255)
                                                                                                                                                                                                                                                           tAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATAttatta                                                                                                                                                                                                                                       >  2:374441/1‑80 (MQ=255)
                                                                                                                                                                                                                                                           tAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATAttatta                                                                                                                                                                                                                                       <  1:374441/80‑1 (MQ=255)
                                                                                                                                                                                                                                                           tAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATg                                                                                                                                          <  2:787810/173‑1 (MQ=255)
                                                                                                                                                                                                                                                           tAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATg                                                                                                                                          >  1:787810/1‑173 (MQ=255)
                                                                                                                                                                                                                                                           tAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTCGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGAAAATATTGATGTTTTCGCGGCTAGATCACAGTGTGTAAATTCATACAGCAAGAGGGAATGCGGATACCTACACTCCAGATTACTACACACTGGAGGCCGACCCTAGGGCGCACAGAATCGAGGCGGAGGAAATTCGGGAAAAGTCTCGGCGGGAAATGTCACCGATGt                              >  2:1133746/1‑281 (MQ=255)
                                                                                                                                                                                                                                                           tAAGCGATGTTAAAAACGAAGCGGTGTCGCTGAAAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTTTTTCCGCAAGAGTGAATGCGGTTACCGACACTCCAGATTACTGACTACTGGAGGCCGACACTATGGAGAACa                                                                                    >  1:697691/1‑227 (MQ=255)
                                                                                                                                                                                                                                                                            gAAGCTGTGTCGCTGACAAGTGAAGGTTTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTACCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGc                                            <  2:258912/250‑1 (MQ=255)
                                                                                                                                                                                                                                                                              aGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCaa                                          >  1:258912/1‑250 (MQ=255)
                                                                                                                                                                                                                                                                              aGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGCCAGCCAGGTCACAGCAAGAAGTTCCga            >  2:444070/1‑279 (MQ=255)
                                                                                                                                                                                                                                                                              aGCGGTGTCGCTGACAAGTGAAATTGTTTGATTATGATAACTAGATTGCACAATATTATTAGCAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGCAGGTAACTCCTTCACGCAAGAGTGAATGCGGTTACCTACACTCCAGATAACTGACCAGTGGAGGCAGACACTATGGCGAACAGCAACCCGCCGGCTGAGATTCGGGAACAGATTTCGCAGGCAACGGTAGCCATGGAACAGAAAGAAGATCAGc            >  2:896727/1‑280 (MQ=255)
                                                                                                                                                                                                                                                                                       gCTGACAAGGAAAAGTTTGTGATTATGATAAGTTGATTGCAAAATATTATTAACAACTAAAGCGATTATTGTACGGAGTAGTGGAGAATATTGATGTTTTCGCGGCGCGATCACAGTGTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCGACACTCCAGCTGACTGACCACTGGAGGCAGACACTATGGCGAACAGCATAACGGCGGATTAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGCCATGTACCAGCAAGAAGTTCCGCAATATGGCAc  <  2:553649/281‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         tGACAATTGAAGTTGTTTGATAATAAGAACTTTAATGCAAAAAATTATTAACAGTTAAAGCAATTGTGTTAAAACAAAATGGATAATATTGATGGTTTCGCGGCGAGAGCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGTTTACCTACACTCCAGATTACTAACCACTGGAGGCAGAAACTATGGCGGACAGCATCACGGCGGATGGGATTCGGGAACAGTTTTCGCAGGCAATGTCAGCCATGTACCAGCAAGAAGTTCCGCAATATGGCAc  <  2:491873/279‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                      
GCGTCAAGAACTCTGACTGCATGCGTTAAAAATTGTTCGCCGGGTAAGGTAAGTTGCGCCCCCTGACGACCACGCTCAAACAAGCGAGCGCCAGTCAGCTGCTCCAGTTCATTCAATGTCTTAGAGAGCGCAGGTTGACTCAAATTAAGGGTTTCAGCCGCGCGCCCCAAAGTTCCTTGTTGTGCGACAGCTACGAATGTATGAAGGTGGCGCAAACGGATGCGCTGACTAAACAGACTATTTTTTTCCATAAGCGATGTTAAAAACGAAGCGGTGTCGCTGACAAGTGAAGTTGTTTGATTATGATAACTTGATTGCAAAATATTATTAACAATTAAAGCAATTATGTTACAGCAAAATGGATAATATTGATGTTTTCGCGGCGAGATCACAGTTTGTAAATTCTTCCCGCAAGAGTGAATGCGGTTACCTACACTCCAGATTACTGACCACTGGAGGCAGACACTATGGCGAACAGCATCACGGCGGATGAGATTCGGGAACAGTTTTCGCAGGCAATGTCAGCCATGTACCAGCAAGAAGTTCCGCAATATGGCAC  >  NC_000913/1494821‑1495379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: