New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4405038	180 (1.210)	4 (0.030)	4/500	13.5	2.2%	coding (352/1299 nt)	purA	adenylosuccinate synthetase
	?	NC_000913	= 4405046	179 (1.230)					coding (360/1299 nt)	purA	adenylosuccinate synthetase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCGATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4404748‑4405038 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑acgcgcTTTCTCACGCGCGTTATCCAGCGCAACGTGATAATCAAGGATCAGCGGACATGCTTCAGACAGCAGCAGACGCTCACGAACGGGGATGCCACGGTCTTCCAGTTCTTTCATCTCTTTCATCAGCGCGGCCGGAGACAGCACAACACCGTTACCGATGATGCTGGTTACATTCTCGCGGAGAATACCTGATGGAATAAGATGGAGAACGGTTTTTTCACCGTTGATTACGAGAGTATGGCCTGCGTTGTGACCGCCCTGGTAGCGTACAACATATTTAGCCCGT  <  NC_000913/4405046‑4404764                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                TCGATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCGGCTGATCCTTGCTTATCACGTTACGCTGGCTAATGC                                                                                                                                                                                                                                                                                               >  2:970081/1‑289       TTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCgcga                                                                                                                                                                                                                                                                                         >  1:703446/1‑285                                                                                                                                                                                                                                               GTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCTTTCTCACGCGCGTTATCCAGCGCAACGTGATAATCAAGGATCAGCGGACATGCTTCAGACAGCAGCAGACGCTCACGAACGGGGATGCCACGGTCTTCCAGTTCTTTCATCTCTTTCATCAGCGCGGCC                                                                                                                                                           >  1:1225054/1‑183                                                                                                                                                                                                                                               GTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGCTTTCTCACGCGCGTTATCCAGCGCAACGTGATAATCAAGGATCAGCGGACATGCTTCAGACAGCAGCAGACGCTCACGAACGGGGATGCCACGGTCTTCCAGTTCTTTCATCTCTTTCATCAGCGCGGCC                                                                                                                                                           <  2:1225054/183‑1                                                                                                                                                                                                                                                                              CACGTTGCGCTGGATAACGCGCTTTCTCACGCGCGTTATCCAGCGCAACGTGATAATCAAGGATCAGCGGACATGCTTCAGACAGCAGCAGACGCTCACGAA                                                                                                                                                                                                             <  1:1265420/102‑1                                                                                                                                                                                                                                                                              CACGTTGCGCTGGATAACGCGCTTTCTCACGCGCGTTATCCAGCGCAACGTGATAATCAAGGATCAGCGGACATGCTTCAGACAGCAGCAGACGCTCACGAA                                                                                                                                                                                                             >  2:1265420/1‑102                                                                                                                                                                                                                                                                                              ACGCGCTTTCACGCGCGCGTTATCCAGCGGAACGTGATAATCAAGGATCAGCGGACATGCTTCAGACAGCAGCAGACGCTCACGAACGGGGATGCCACGGTCTTCCAGTTCTTGCATCTCTTTCATCAGCGCGGTCGGAGACAGCACAACACCGTTACCGATGATGCTGGTTGCATTCTCGCGGAGAATACCTGATGGAATAAGATGGAGAACGGTTTTTTCACCGTTGATTACGAGAGTATGGCCTGCGTTGTGACCGCCCTGGTAGCGTACAACATATTTAGCCCGT  <  2:187183/289‑1                                                                                                                                                                                                                                                                                               CGCGCTTTCTCACGCGCGTTATCCAGCGCAACGTGATAATCAAGGATCAGCGGACATGCTTCAGACAGCAGCAGACGCTCACGAACGGGGATGCCACGGTCTTCCAGTTCTTTCATCTCTTTCATCAGCGCGGCCGGAGACAGCACAACACCGTTACCGATGATGCTGGTTACATTCTCGCGGAGAATACCTGATGGAATAAGATGGAGAACGGTTTTTTCACCGTTGATTACGAGAGTATGGCCTGCG                                         >  1:251348/1‑249                                                                                                                                                                                                                                                                                               CGCGCTTTCTCACGCGCGTTATCCAGCGCAACGTGATAATCAAGGATCAGCGGACATGCTTCAGACAGCAGCAGACGCTCACGAACGGGGATGCCACGGTCTTCCAGTTCTTTCATCTCTTTCATCAGCGCGGCCGGAGACAGCACAACACCGTTACCGATGATGCTGGTTACATTCTCGCGGA                                                                                                          >  1:347998/1‑184                                                                                                                                                                                                                                                                                               CGCGCTTTCTCACGCGCGTTATCCAGCGCAACGTGATAATCAAGGATCAGCGGACATGCTTCAGACAGCAGCAGACGCTCACGAACGGGGATGCCACGGTCTTCCAGTTCTTTCATCTCTTTCATCAGCGCGGCCGGAGACAGCACAACACCGTTACCGATGATGCTGGTTACATTCTCGCGGA                                                                                                          <  2:347998/184‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                TCGATCTTCTGACTGAACGGGCTAAATATGTTGTACGCTACCAGGGCGGTCACAACGCAGGCCATACTCTCGTAATCAACGGTGAAAAAACCGTTCTCCATCTTATTCCATCAGGTATTCTCCGCGAGAATGTAACCAGCATCATCGGTAACGGTGTTGTGCTGTCTCCGGCCGCGCTGATGAAAGAGATGAAAGAACTGGAAGACCGTGGCATCCCCGTTCGTGAGCGTCTGCTGCTGTCTGAAGCATGTCCGCTGATCCTTGATTATCACGTTGCGCTGGATAACGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4404748‑4405038 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑acgcgcTTTCTCACGCGCGTTATCCAGCGCAACGTGATAATCAAGGATCAGCGGACATGCTTCAGACAGCAGCAGACGCTCACGAACGGGGATGCCACGGTCTTCCAGTTCTTTCATCTCTTTCATCAGCGCGGCCGGAGACAGCACAACACCGTTACCGATGATGCTGGTTACATTCTCGCGGAGAATACCTGATGGAATAAGATGGAGAACGGTTTTTTCACCGTTGATTACGAGAGTATGGCCTGCGTTGTGACCGCCCTGGTAGCGTACAACATATTTAGCCCGT  <  NC_000913/4405046‑4404764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.