New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1828681	89 (0.660)	4 (0.030)	4/468	NT	4.5%	coding (1054/1479 nt)	astD	succinylglutamic semialdehyde dehydrogenase
	?	NC_000913	= 1828695	86 (0.660)					coding (1040/1479 nt)	astD	succinylglutamic semialdehyde dehydrogenase
Rejected: Frequency below/above cutoff threshold.

GCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1828395‑1828681 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccctgcttgCGCCGCGCTTATTACAAGCAGGGACATCGTTGCTGACGCCGGGGATCATTGAAATGACAGGCGTTGCTGGCGTACCAGATGAAGAGGTGTTCGGACCGTTATTGCGCGTCTGGCGTTATGATACTTTCGATGAAGCGATTCGAATGGCGAATAACACTCGCTTCGGACTCTCTTGCGGTCTGGTTTCCCCCGAGCGGGAAAAGTTCGATCAACTGTTGCTGGAGGCGCGGGCGGGGATTGTTAACTGGAACAAACCGCTTACCGGTGCTGCCAGTACCG  <  NC_000913/1828695‑1828417                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        GCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCC                                                                                                                                                                                                                                                                                                <  1:382918/280‑1     CAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCT                                                                                                                                                                                                                                                                                           <  1:1351163/281‑1      AATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTGGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCACCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGCTCCCCGGCGTCAGCAACGATGGCCCTGCT                                                                                                                                                                                                                                                                                           >  1:335660/1‑280                                  GCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTC                                                                                                                                                                                                                                                                                                 <  1:1117976/246‑1                                                                                            GATCGAACTTTTCCCCCTCGGGGGAAACCAGGCCGCAATAGAGTCCGAAGCGAGTTTTATTCGCCATTCGAATCGCTTCATCGAAAGTGTCATAACGCCAGACACACAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCAGCGCAAGCAGGGACATCGTTGCTGACGCCGGGGATCATTGAAATGACAGGCGTTGCTGGCGTACCAGATGAAGA                                                                                                                                                                                                    <  1:231464/281‑1                                                                                                  ACTTTTCCCCCGCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTAGTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGACATCGTTGCTGACGCCGGGGATCATTGAAATGACAGGCGTTGCTGGCGTACCAGATGAAGAGGTGTT                                                                                                                                                                                              <  1:1361654/281‑1                                                                                                                                                                                                                                               ccCCTGTTCTTTCAATGACCCCCGGCGGCACCCACGATGTCCCTGCTTGGGCCGCGCTTATTACAAGCAGGGACATCGTTGCTGGACCCGGGGATCATTGAAATGGCAGGCGTTGCTGGCGTGCCAGATGAAGAGGTGTTCGGACCGTTATTGCGCGTCTGGCGTTATGATACTTTCGATGAAGCGATTCGAATGGCGAATAACACTCGCTTCGGACTCTCTTGCGGTCTGGTTTCCCCCGAGCGGGAAAAGTTCGATCAACTGTTGCTGGAGGCGCGGGC                                                 <  2:1361654/279‑1                                                                                                                                                                                                                                                    GTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGCGCCGCGCTTATTACAAGCAGGGACATCGTTGCTGACGCCGGGGATCATTGAAATGACAGGCGTTGCTGGCGTACCAGATGAAGAGGTGTTCGGACCGTTATTGCGCGTCTGGCGGTATGATACTTTCGATGAAGCGATTCGAATGGCGAATAACACTCGCTTCGGACTCTCTTGCGGGCTGGTTTCCGCCGAGCGGGAAAAGGTCGTTCAACTGTTGCTGGAGGCGGGGGCGGGGg                                            >  2:107743/1‑280                                                                                                                                                                                                                                                                                              TGCGCCGCGCTTATTACAAGCAGGGACATCGTTGCTGACGCCGGGGATCATTGAAATGACAGGCGTTGCTGGCGTACCAGATGAAGAGGTGTTCGGACCGTTATTGCGCGTCTGGCGTTATGATACTTTCGATGAAGCGATTCGAATGGCGAATAACACTCGCTTCGGACTCTCTTGCGGTCTGGTTTCCCCCGAGCGGGAAAAGTTCGATCAACTGTTGCTGGAGGCGCGGGCGGGGATTGTTAACTGGAACAAACCGCTTACCGGTGCTGCCAGTACCG  <  1:551520/281‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        GCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCTCCAGCAACAGTTGATCGAACTTTTCCCGCTCGGGGGAAACCAGACCGCAAGAGAGTCCGAAGCGAGTGTTATTCGCCATTCGAATCGCTTCATCGAAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1828395‑1828681 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccctgcttgCGCCGCGCTTATTACAAGCAGGGACATCGTTGCTGACGCCGGGGATCATTGAAATGACAGGCGTTGCTGGCGTACCAGATGAAGAGGTGTTCGGACCGTTATTGCGCGTCTGGCGTTATGATACTTTCGATGAAGCGATTCGAATGGCGAATAACACTCGCTTCGGACTCTCTTGCGGTCTGGTTTCCCCCGAGCGGGAAAAGTTCGATCAACTGTTGCTGGAGGCGCGGGCGGGGATTGTTAACTGGAACAAACCGCTTACCGGTGCTGCCAGTACCG  <  NC_000913/1828695‑1828417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.