New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4388347 =146 (1.090)4 (0.030) 4/480 NT 2.7% coding (1024/3324 nt) mscM mechanosensitive channel protein, miniconductance
?NC_000913 4388514 = 142 (1.070)coding (857/3324 nt) mscM mechanosensitive channel protein, miniconductance
Rejected: Frequency below/above cutoff threshold.

GCAGAAAACAGCGCCGATTTGCCGAAAGATATCGTCGCGCAATTCAAAATTAACCGCGAACTATCGGCGGCTTTGAATCAACAGGCGCAGCGGATGGATCTCGTTGCCTCGCAACAGCGTCAGGCTGCCAGCCAGACGTTACAGGTCCGGCAGGCGTTGAATACGCTGCGTGAACAGTCGCAATGGCTGGGATCGTCCAATCTGCTCGGCGAAGCGCTGCGGGCGCAGGTGGCACGGCTGCCGGAAATGCCGAAACCACAACAGCTTGATACCGAAATGGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4388629‑4388347
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAGCGCGCTAATTCCTGGCGGTTATTGGCAGACAGCTGCG  >  NC_000913/4388514‑4388793
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
GCAGAAAACAGCGCCGATTTGCCGAAAGATATCGTCGCGCAATTCAAAATTAACCGCGAACTATCGGCGGCTTTGAATCAACAGGCGCAGCGGATGGATCTCGTTGCCTCGCAACAGCGTCAGGCTGCCAGCCAGCCGTTACAGGCCCGGCAGGCGTTGAATACGCTGCGTGAACAGTCGCAATGGCTGGGATCGTCCAATCTGCTCGGCTAAGCGGTGCGGGCGCAGGGGGCCGGGGCGCCGGAAATTGCGAAACCACAACAGCTTGATACCGAAATGGC                                                                                                                                                                                                                                                                                            >  2:662853/1‑281
  AGAAAACAGCGCCGATTTGCCGAAAGATATCGTCGCGCAATTCAAAATTAACCGCGAACTATCGGCGGCTTTGAATCAACAGGCGCAGCGGATGGATCTCGTTGCCTCGCAACAGCGTCAGGCTGCCAGCCAGACGTTACAGGTCCGGCAGGCGTTGAATACGCTGCGTGAACAGTCGCAATGGCTGGGATCGTCCAATCTGCTCGGCGAAGCGCTGCGGGCGCAGGTGGCACGGCTGCCGGAAATGCCGAAACCACAACAGCTTGATACCGAAATGGCG                                                                                                                                                                                                                                                                                           >  1:649975/1‑280
  AGAAAACAGCGCCGATTTGCCGAAAGATATCGTCGCGCAATTCAAAATTAACCGCGAACTATCGGCGGCTTTGAATCAACAGGCGCAGCGGATGGATCTCGTTGCCTCGCAACAGCGTCAGGCTGCCAGCCAGACGTTACAGGTCCGGCAGGCGTTGAATACGCTGCGTGAACAGTCGCAATGGCTGGGATCGTCCAATCTGCTCGGCGAAGCGCTGCGGGCGCAGGTGGCACGGCTGCCGGAAATGCCGAAACCACAACAGCTTGATACCGAAATGGCG                                                                                                                                                                                                                                                                                           <  2:649975/280‑1
                                                                                                                                     AGACGTTACAGGTCCGGCAGGCGTTGAATACGCTGCGTGAACAGTCGCAATGGCTGGGCTCGTCCAATCTGCTCGGCGAAGCGCTGCGGGCGCAGGTGGCACGGCTGCCGGAAATGCCGAAACCACAACAGCTTGATACCGAAATGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCCGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTAC                                                                                                                                                        >  1:95136/1‑280
                                                                                                                                      GACGTTACAGGTCCGGCAGGTGTTGAATACGCTGCGTGAACAGTCACAATGGCTGGGATCGTCCAATCTGCTCGGCGAAGCGCTGCGGGCGCAGGTGGCACGGCTGCCGGAAATGCCGAAACCACAACAGCTTGATACCGAAATGGCGCTGTTGCGAGGCAACTAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTACTTTCGAATTGACCGACGAGCTCTTTGGGCAAATCGGACCTGTTTTCTGCCAGCAATTGGGTACT                                                                                                                                                       >  2:1352849/1‑280
                                                                                                                                                                                                         CTGCTCGGCGAAAGGCTGCGGGGGCAGGTGGCACGGCTGCCGGAAATGCCGAAACCACAACAGCTTGATACCGAAATGGCGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACG                                                                                   <  1:1352849/281‑1
                                                                                                                                                                                                                                                      tagaCGAAACCCCAACGGCTTGATACCGAAATGCCGCTGTTGCGAGGAAACGGGATCCTTCCGCTGCGCCTGTTGATTCAAGGCCGCCAATATTTCGCGGTTAATTTTTAATTGCGCGACGATATCTTTCGTCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCGCCGCCTCAAGCTGACTGTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAGC                                      <  2:95136/277‑1
                                                                                                                                                                                                                                                                                  ggcTGGTGCTGTTGGGAGGCAACGAGATCCAGCCGGTGCGCCTGTTGTTTCAAAGCGTGCGATAGTTCGCGGTTAATTTTTAATTGCGCGACGATATCTTGCGGCAAATCGGCGCTGTTTTCTGCCAGCTATTCGGTACTTGCCAGCTCGCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGGGCGTAAGCGCGCTAATTCCTGGCGGTTATTGGCAG           <  2:1162813/277‑1
                                                                                                                                                                                                                                                                                          CTGTTGCGAGACAACGAGATCCATCCTCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAGCGCGCTAATTCCTGGCGGTTATTGGCAGACAGCTGCG  <  1:671694/281‑1
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
GCAGAAAACAGCGCCGATTTGCCGAAAGATATCGTCGCGCAATTCAAAATTAACCGCGAACTATCGGCGGCTTTGAATCAACAGGCGCAGCGGATGGATCTCGTTGCCTCGCAACAGCGTCAGGCTGCCAGCCAGACGTTACAGGTCCGGCAGGCGTTGAATACGCTGCGTGAACAGTCGCAATGGCTGGGATCGTCCAATCTGCTCGGCGAAGCGCTGCGGGCGCAGGTGGCACGGCTGCCGGAAATGCCGAAACCACAACAGCTTGATACCGAAATGGCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4388629‑4388347
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACTTTCCAGCGCCCGCTCCGCCTCAAGCTGACGTTGGCTGTTTAATTGATTACGCAAGGCCTGCAAATACGCATCCAGTTGCTGGCTCTCTTTTTCCGCCAGCTCTGAGCGTAAGCGCGCTAATTCCTGGCGGTTATTGGCAGACAGCTGCG  >  NC_000913/4388514‑4388793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.