New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3505613	61 (0.650)	3 (0.030)	3/470	9.1	5.0%	coding (408/1086 nt)	yhfS	FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
	?	NC_000913	3505821 =	56 (0.640)					coding (200/1086 nt)	yhfS	FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGAT  >  NC_000913/3505549‑3506088                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                            ccAGCACATCTTCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCTCCATCTTCTCAATAATAACCCGTGTCGTCGGATAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCTCCAGCCCTCGCTCCCTGCACCAGCgccgccgcc                                                                                                                                                                                                                                                                           >  1:638092/1‑275 (MQ=255)             ccAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGCCAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGATGCCCCGGTTTGAGAAAAGCCGCCAGCCCGGCGCGAATAGCGCCAGTCCCCGCCCCCTGCACCAGCCCCCCAGCCTGTTCGGGaaa                                                                                                                                                                                                                                                                >  2:58968/1‑274 (MQ=255)                gCACGTAGCTGTCCTGCGGCTGCGGGCGCGTATGATGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGCCCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAAGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCa                                                                                                                                                                                                                                                            <  1:716780/275‑1 (MQ=255)                                     gctgGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCCCAATAATAACCCGTGCCGTCGGGTAAACAGGCGCGTCATGCACCCGAAGACTCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCCACCGCGCCGCAGCCTGCGCGGGAAAGGCATCAGCCACCACCTGTGcctc                                                                                                                                                                                                                                        >  2:184796/1‑272 (MQ=255)                                                   ggaaagcgcgcGGCACCCGTTGGTGACTGGTGGACGACCGGCGTAAGTCCCGAAAGGTCATGCACACAAACAGTACGAGGCGTCAGGCCCAGCTGCACAGTAATAACACGTTTCATCGGTGAACCGGGCGCGTCAGGCACCAAAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCACGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAAcgc                                                                                                                                                                                                                          <  2:139186/267‑1 (MQ=255)                                                       ccAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATcagcc                                                                                                                                                                                                                                                      <  1:849300/242‑1 (MQ=255)                                                           cgccgcATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGAt                                                                                                                                                                                                                 >  1:824306/1‑275 (MQ=255)                                                           cgccgaATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGAt                                                                                                                                                                                                                 <  1:773430/275‑1 (MQ=255)                                                                          ttgCTCGTCGACGACCTGCTTCAGGGCCAAAAGGTCATTGAAAGCAACGGGAATAAGCGTCAGCCCCATACGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCAGGCACCAGAAGACGCGGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCGGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGtt                                                                                                                                                                                                   <  2:776636/274‑1 (MQ=255)                                                                          ttgCTCATCGCCTGCCTGCTTCAGTGCCGAAAGGTACTTAAAATCAAAAGTAATAAGCGCCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGGCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGTCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGtt                                                                                                                                                                                                   <  2:822178/274‑1 (MQ=255)                                                                          ttgATCGTCGACGGCCTGCTTCAGGGCGCACAGGTCATTGAAATCAACAGTACTAAGCGTCAGCCCCGTCTACTCAATAATAACCCGTGTCGTCGGGTAAACGGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTGTGAGCAAAGCCGCCAGCCCGGCGCGAACCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGtt                                                                                                                                                                                                   <  2:368889/274‑1 (MQ=255)                                                                          tcgcgactcgACGCCCGGCTTCCGGGCCGACACGTCATTGAAATCAACAGGAATAAGCGTCAGCCCCATCTGCTCAGTAATAACCCGGGTCGTCGGGTAACCAGGCGCGTCATGCACCAGAAGACGCGCCCCCGGGTTGAGCAAGGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGtt                                                                                                                                                                                                   <  2:817011/267‑1 (MQ=255)                                                                                         cTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGcc                                                                                                                                                                                                                                                                           <  1:927641/187‑1 (MQ=255)                                                                                                    ccGACAGGTCAAATAAATCAACAGTAATAAGCGTCCGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGTTAAACAGGCTCTTCATCCATCAGAAAACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGAGCGAAACGCGCCACTCCCCGCCCCCAGCACCAACGCCGCAGCCTGCCCGCGAAACGCCTCAGCCACCACCTGTACAGCACGCTGGGTAACGCTCGGTTGACTCACTCCCGCCGCTAAACCTAAATCACCTGCATCAAGAAAc                                                                                                                                                                        >  2:698135/1‑275 (MQ=255)                                                                                                     cGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACt                                                                                                                                                                       <  1:58968/275‑1 (MQ=255)                                                                                                                     tCAACAGTAATAAGCGTCAGCCTCATCTGCCAAATAATAACCCGTGCCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGAAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCGCCAGCGCCGCAGCCGGTGCGTGAAATGCACCAGCCAGCCCCCGTTCCAAACGCCGGGTAAGGCGCGATGGCTTAAGTCCTGTCTGTAAGCCACATTCCCCCCCAGTAAGAAAACCGCGCCTGGGGAAAt                                                                                                                                                        >  2:740999/1‑274 (MQ=255)                                                                                                                      cAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCAAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGCAATGCACCAGCCAGCACCTGTTCCACACGTTGGGTAACGCGCGGTGGCTCCCGTCCTGGCGTTAAGCCCAAATCACCGCCCGCAAGCAAACCGCTGCCGGGGAAATgg                                                                                                                                                      >  2:659017/1‑275 (MQ=255)                                                                                                                       aaCAGTAATTAGCGTCAGCCCCAGCTGCTCAAGAACAACCCGTGTCGTCGGGTAAACAGCCGCGTCATGCACCAAAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGc                                                                                                                                                     <  1:887283/275‑1 (MQ=255)                                                                                                                              ataaGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCCGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACCCGCTGGGCAACGCGCGGTTGATTCAGTCTCGGCGTTAAGCCTAAATCACCGCCCGTAAGAAACTCGCTGCAGGGGAAATGGCGACaaa                                                                                                                                               >  2:786236/1‑274 (MQ=255)                                                                                                                                 aGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCCCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCACTGCCGGGGAAATGGCGAAAAATGCt                                                                                                                                           >  1:768216/1‑275 (MQ=255)                                                                                                                                      cAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGa                                                                                                                                                   >  2:629156/1‑262 (MQ=255)                                                                                                                                          cccATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACaaa                                                                                                                                               <  1:629156/262‑1 (MQ=255)                                                                                                                                                  cTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCCCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGCAACTCGCTGCCGGGGCAATGGCGACAAATGCAATCCACCAGCGCcaaa                                                                                                                           >  2:829587/1‑273 (MQ=255)                                                                                                                                                            cacgccgtggccgGGAAGACAGGCGCGTCCGTCGCCATAAGCCGCGGCCCCGGGTGGTCCCACCCCACCAGGCCGGCGCGGTTCGCGCCGGTCCCCGCGCCCTGCACCAGCGGCTCAGCCTGTGCGTGAAATGCATCATCCAGCACCTGTACCATACGCTGGTTATCGCGCGGTTGATTCAGGCCTGGCGGTAAGCCTAAATCACCGCCAGTAAGAAACTCGCGGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTtgctg                                                                                                                <  2:200300/264‑1 (MQ=255)                                                                                                                                                            aCCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGACATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTtgct                                                                                                                 >  2:627264/1‑274 (MQ=255)                                                                                                                                                             cccGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCAGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTtgctg                                                                                                                >  1:2000/1‑274 (MQ=255)                                                                                                                                                                gtgcCGTCGGGGACACAGGCGCGGCATGCACCAGAGGCCACTGCCCAGGTGTGATCACGTCCGCCAGCCCCGCGCGGATCGGTCCAGTCCCCACGCCCAGCACCAGCGCGGGAGGCTGTGCGTAGAATGCATCGGCCAGCACCTGTGCCACACGTTGGGTAACGCGCGGTTGAGTCAGTCCTGGCGTTAAGCCTAAAGCACCGCCAGTAAGAAACTCGCTGCCGGTGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTgcgc                                                                                                             <  2:158375/274‑1 (MQ=255)                                                                                                                                                                 tgtCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCCTCAGCCAGCACCTGTTCCACCCGCTGGGTAACGCGCGGTTGCTTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACACGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCt                                                                                                           >  1:46167/1‑275 (MQ=255)                                                                                                                                                                 tgtCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGtt                                                                                                                                                                                                                    >  2:529042/1‑170 (MQ=255)                                                                                                                                                                 tgtCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCCCCGCCAGTAAGAAACTCGCTGCCGGGGCAATGGCGACAAAAGCCATCCACCACCCCAAACTGCTTTTGCTGCGcc                                                                                                            >  2:868346/1‑274 (MQ=255)                                                                                                                                                                 tgtCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCAACCAGCGCAAACTGCTTTtgctggcccc                                                                                                           >  2:4410/1‑270 (MQ=255)                                                                                                                                                                 tgtCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGATAGCCAACAGCCAAAACTGCTTTTGGTTCGCCt                                                                                                           >  2:23188/1‑275 (MQ=255)                                                                                                                                                                 tgtCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGCGAAATGCATCAGCCAGCACCTGTTCCCAACGTTGGGTAACGCGCGGTTGATTCACTCCTGGCGCTAAGCCTAAATCACCGCCCGAAAGAAACCCGCTGCCGGGGAAATGGCGACAACATCTATCCACCCGCGCAAACTCCTTTTCCTGcccca                                                                                                           >  2:121647/1‑274 (MQ=255)                                                                                                                                                                     gtcgGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGAtt                                                                                                                                                                                                                <  1:529042/170‑1 (MQ=255)                                                                                                                                                                            aaaCAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTc                                                                                                <  1:868222/275‑1 (MQ=255)                                                                                                                                                                                  gcgcGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGACCTGCACCAGCGCCGCAGCCCGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTTATTCAGTCCTCGCGTTAAGCCAAAATCACCCCCAGTAAAAAACTCGCTGCCGGGGAAATGGCGACAAATGCTAGCCAACGGCCCAAACTGCTTTCGCTCCGCACCAATAATCGGCAGGCtt                                                                                          >  2:326343/1‑275 (MQ=255)                                                                                                                                                                                     cgTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCCAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTg                                                                                        <  1:440642/274‑1 (MQ=255)                                                                                                                                                                                       gcATGCACCGCAGGAGGCTGCCCGGGGGTGGGCAGAGCCGCCAGCCCGGCTCGAGTCGCGCCAGTCGCCCCGCCCAGCACCGGGGCCGCAGCTTGTGCGTGAAGTGCATCGGCCACGACCCGTGCCGCACGCTGGGTAACGCTGGGGTGATTCAGTCCTGGCGTTAAGCCTAAATCTCCGCCAGTAAGAAACTCGCGGGGGGGGAAGTGGGGACAAATGCTATCCACCAGCGGAGGCTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCa                                                                                      <  2:65048/273‑1 (MQ=255)                                                                                                                                                                                           gCACCACAGGGCGCGGCCCCGGTTTGAGCAAAGCCGCCCGCCCGGCGGGAATCGAGCCAGTCCCCGCGCCCTGAACCAGCGCCGCAGGCTTTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGa                                                                                 <  2:768216/275‑1 (MQ=255)                                                                                                                                                                                                agaagaCGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCCGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGATTTTGCTGCGCCTCAATAAACGTCAGGCTTTGCAGAGGAAAAGt                                                                            >  2:879029/1‑275 (MQ=255)                                                                                                                                                                                                 gaggccGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAAc                                                                              <  1:868346/267‑1 (MQ=255)                                                                                                                                                                                                       gCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCa                                                                                                                                                                                       >  2:466562/1‑161 (MQ=255)                                                                                                                                                                                                        cgaccccCGGTTGGGCAAAGCCGCCAGCCCGGGGCGAATCGCGCCAGTCCCCGCGCCCTGGACCAGCGCCGCAGCCTGGGCGTGAAATGCGTCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATGCAGTCGTGGCGTTAAGCCTGAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAg                                                                    <  2:7389/272‑1 (MQ=255)                                                                                                                                                                                                        cTGCCCCGGTTTGAGCAAAGCCACCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCCTCATa                                                                     >  1:479230/1‑274 (MQ=255)                                                                                                                                                                                                         tGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTTCCGGGGCAAAGGCGATAAATGCCAACCACCAACGCAAACTGATTTTGTCGCGCCTCAACAATCGTCCGGCATTGCAGAGGAAACGTCTTTATAg                                                                    >  2:419114/1‑274 (MQ=255)                                                                                                                                                                                                         tGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCGTTGCAGAGGAAACGTCTTCATAg                                                                    >  2:773705/1‑274 (MQ=255)                                                                                                                                                                                                           ccccGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGc                                                                                                                                                                                   <  1:466562/161‑1 (MQ=255)                                                                                                                                                                                                                           gccgccAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGga                                                 <  1:579999/275‑1 (MQ=255)                                                                                                                                                                                                                                cAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCGCCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGgaacgaa                                            <  1:740999/275‑1 (MQ=255)                                                                                                                                                                                                                                cAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGgaacgaa                                            <  1:121647/275‑1 (MQ=255)                                                                                                                                                                                                                                cAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGgaacgaa                                            <  1:627264/275‑1 (MQ=255)                                                                                                                                                                                                                                     cGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGc                                       <  1:4410/275‑1 (MQ=255)                                                                                                                                                                                                                                          cgAATCGCGCCCGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGc                                  <  1:879029/275‑1 (MQ=255)                                                                                                                                                                                                                                                         cccACGCCCTGAACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTAGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTACTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACAtt                    <  1:326343/274‑1 (MQ=255)                                                                                                                                                                                                                                                              gcCATGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCGAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTcagcag              <  1:659017/275‑1 (MQ=255)                                                                                                                                                                                                                                                              gaccTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAACCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTcagcag              <  1:352889/273‑1 (MQ=255)                                                                                                                                                                                                                                                                ccagCACCAGCGCCGAAGGCTGTGCGTGAAATGCGGCAGCCAGCACCTGTGCCAAACGCTGGGTAACGGGCGGTTGATTCAGGCGTGGCTTTAAGCCTAAATCACCGCCAGTAAGAAACTCGATGGCGGGGAAAGGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCTCCTCAATATTCGTCAGGCTATGCAGAGGAAACGTCTTCATAGGTGCCTTCCTAAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGaa            <  2:661517/272‑1 (MQ=255)                                                                                                                                                                                                                                                                 cTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCGATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGTCGGAACGAACAGGCCAAGCCAGGCAAGAACAttaacacgaa            >  2:876334/1‑266 (MQ=255)                                                                                                                                                                                                                                                                  tGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATa          <  1:829587/275‑1 (MQ=255)                                                                                                                                                                                                                                                                  tGCACCAGCGCCGCAGCCCGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGAAAACTGCTTTTGCTGCGCCCCAATAATCCTCAGCCTTTTCATCGGAAACGTCTTCTTAGGGTCCTTCCTTGTTCCGCAAAGCACTGGCAAAGCCAGGCAAAAACACTCACCaaaa            >  2:700063/1‑273 (MQ=255)                                                                                                                                                                                                                                                                     cccggggcggcggCCTGTGCTTGAAATGCATCAGCCAGCACCTGTTCCCCACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCg       <  2:824306/263‑1 (MQ=255)                                                                                                                                                                                                                                                                      ccAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATAc         <  1:184796/272‑1 (MQ=255)                                                                                                                                                                                                                                                                       cAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGt     <  1:23188/275‑1 (MQ=255)                                                                                                                                                                                                                                                                          cgccgcAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTa                             >  2:101522/1‑248 (MQ=255)                                                                                                                                                                                                                                                                          cgccgcAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTCTGCTGCGCCTCAATCATCGCCAGGCTTTGCAGAGCAAACGTCTTCATCGGTGCCTTCCTTAAGCCGGAACGCACAGGCCAAGCCAGTAAAAAACATTCAGCAGAATACCggggct  >  2:130484/1‑271 (MQ=255)                                                                                                                                                                                                                                                                            ccgcAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGc                                  <  1:539184/241‑1 (MQ=255)                                                                                                                                                                                                                                                                            ccgcAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGc                                  >  2:539184/1‑241 (MQ=255)                                                                                                                                                                                                                                                                              gcAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGa                         <  1:101522/248‑1 (MQ=255)                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                            CCAGCACATCTGCCAGCACGTAGCTGTCCTGCGGCTGCTGGCGCGTATGCTGCACCAGCGCCGCATCCGGTTGTTGCTCGTCGACGACCTGCTTCAGTGCCGACAGGTCATTGAAATCAACAGTAATAAGCGTCAGCCCCATCTGCTCAATAATAACCCGTGTCGTCGGGTAAACAGGCGCGTCATGCACCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGTCCTGGCGTTAAGCCTAAATCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGCCTTCCTTAAGCCGGAACGAACAGGCCAAGCCAGTAAAGAACATTCAGCAGAATACCGGTGAT  >  NC_000913/3505549‑3506088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.