New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 349741557 (0.740)9 (0.120) 9/450 4.9 13.5% coding (413/807 nt) nirC nitrite transporter
?NC_000913 3497429 = 58 (0.750)coding (427/807 nt) nirC nitrite transporter
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

TGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3497184‑3497415
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGGCTAAAACCACTGCACCGGCAATGGTACTCTTCTTCAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGTTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTAC  >  NC_000913/3497429‑3497653
                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
TGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTCTGGCTAAAAC                                                                                                                                                                                                                        >  2:98888/1‑243
              gtTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTCTGGCTAAAACCACTGCACCGGCAATGGTACTC                                                                                                                                                                                                  <  2:800922/249‑1
                 ATCGCCTTAACGCTGGTGATTATTGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGAAAACCTGGCTGGGTAACCTGCTCGGTTCCGTCTTAGTTGCCATGCTCTATAGCTGTGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTCTGGCTAAAACCCCTGCACCGGCAAGGGTGCTCTTC                                                                                                                                                                                               >  2:529496/1‑251
                                                                               CACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTCTGGCTAAAACCACTGCACCGGCATTGGTACTCTTCTTCAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTCGCAATACGGATGGCGCTGCGCACTGA                                                                                                                                 >  2:291627/1‑251
                                                                                      GGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTCTGGCTAAAACCACTGCACCGGCAATGGTACTCTTCTTCAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCG                                                                                                                          >  2:281456/1‑251
                                                                                                                        TGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGTTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTCTGGCTAAAACCACTGCACCGGCAATGGTACTCTTCTTCAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGG                                                                                        <  1:291611/251‑1
                                                                                                                                        GCAAACCTGGCTGGGTAACCTGGTCGGTTCCCTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTCTGGCTAAGACCACTGCACCGGCAATGGTACTCTTCTTCAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGG                                                                        <  1:281442/251‑1
                                                                                                                                                                                                  GGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGTCTGGCTAAAACCACTGCACCGGCAATGGTACTCTTCTTCAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTCTCTGGAGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGTTCGCGCTCTCCTGGTTCGGCAACCAC              >  2:337724/1‑251
                                                                                                                                                                                                              CTGCGGCCGGTAGATACCAGCATCGTCTGGCGAAAACCACTGCACCGGCAATGGTACTCTTCTTCAAAGGTGCATTGTGTAACTGGCTGGTGTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGTTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTAC  <  1:529465/251‑1
                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
TGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTGAACTGTTCACCGGACACACCATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGGTAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3497184‑3497415
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTGGCTAAAACCACTGCACCGGCAATGGTACTCTTCTTCAAAGGTGCATTGTGTAACTGGCTGGTTTGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTATCGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGTTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTAC  >  NC_000913/3497429‑3497653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.