BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,183,214	C→T	T657I (ACC→ATC)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,183,214	0	C	T	99.3%	1071.5 / NA	287	T657I (ACC→ATC)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (140/143); total (141/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

GTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAG  >  NC_000913/4182969‑4183459

  ccGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCcagcag                                                                                                                                                                                                                                                <  2:1761680/251‑1 (MQ=255)

      gtACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGGCCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAggtgg                                                                                                                                                                                                                                            <  2:1269704/251‑1 (MQ=255)

       tACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAggtggt                                                                                                                                                                                                                                           <  1:671474/251‑1 (MQ=255)

            acaGACTAACGAATACGGTTTCCTTGAGACTCCGTATCGTACAGTGACCGACGGAGTAGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATGGCCCAGGAGAACTCCAACATGGATGAAGCAGGCCACTTCGTAGAAGATCTGGTAACTTGCAGTAGCAAAGGAGAATCAAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTTTCCATCCCGCAGGTGGTATCCg                                                                                                                                                                                                                                      >  1:766076/1‑251 (MQ=255)

                  tAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGCCTACATGGACGTATCCATCcagcag                                                                                                                                                                                                                                                >  2:1660840/1‑235 (MQ=255)

                  tAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTAACCATCCAGCAGGTGGGATCCGTCGGTg                                                                                                                                                                                                                                >  2:659069/1‑251 (MQ=255)

                  tAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCAGCAAGGTTGACTACATGGACGTATCCATCAAGCAGGTGGTATCCGTCGGtt                                                                                                                                                                                                                                >  2:171415/1‑250 (MQ=255)

                    aCGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCg                                                                                                                                                                                                                              >  1:276211/1‑251 (MQ=255)

                      gAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTACCTTGCCGTAGCAAAGGCGAATCCAGCTTGATCAGCAGCGACCCGGTTGACTACATGGACGTATCAATCCAGCAGGTGGTATc                                                                                                                                                                                                                                        >  2:1550780/1‑239 (MQ=255)

                            ggCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGCTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGACGGTATCCGTCTGTTCGTCCCttan                                                                                                                                                                                                                      >  2:1320919/1‑248 (MQ=255)

                             gCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATc                                                                                                                                                                                                                     >  1:2048991/1‑251 (MQ=255)

                                tgtgtagaagaggcagtcggaacgggACCGACGGTGTTGTAACTCGCGAAATTCACTGCCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACGTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCg                                                                                                                                                                                                                  <  2:262729/226‑1 (MQ=255)

                                tCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCg                                                                                                                                                                                                                  <  1:1716196/251‑1 (MQ=255)

                                  ctggagaCTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGtt                                                                                                                                                                                                                <  2:428151/248‑1 (MQ=255)

                                       gaCTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTc                                                                                                                                                                                                                                    >  2:1209390/1‑226 (MQ=255)

                                       gaCTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTgg                                                                                                                                                                                                           >  1:2306341/1‑251 (MQ=255)

                                       gaCTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAAATACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGCTCCCGTTCCTgg                                                                                                                                                                                                           >  2:1355143/1‑251 (MQ=255)

                                       gaCCCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTTCGTCCCTGATCCCGTTCCTgg                                                                                                                                                                                                           >  1:437134/1‑251 (MQ=255)

                                          tCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAac                                                                                                                                                                                                        <  1:1504887/251‑1 (MQ=255)

                                          tCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAac                                                                                                                                                                                                        <  1:1766139/251‑1 (MQ=255)

                                          gacagaTCGTAAAGTGACCGAGGGTGTTTTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAac                                                                                                                                                                                                        <  2:1770803/246‑1 (MQ=255)

                                           ccGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAaca                                                                                                                                                                                                       >  2:705699/1‑251 (MQ=255)

                                            cGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCATGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGACGACCTGGGAACTGGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGCCCCGGTTGAGTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAacag                                                                                                                                                                                                      <  2:2221178/251‑2 (MQ=255)

                                                     aaGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGCAGACCTGGTAACTTGCCGTAGAAAAGGCGAATACAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATACGGCAGGTGGTATCCGTCGGTGCGTCCCTGACCCCGTTCCTGGAACCAGATGACGcc                                                                                                                                                                                             >  2:228019/1‑251 (MQ=255)

                                                     aaGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGcc                                                                                                                                                                                             >  1:1912169/1‑251 (MQ=255)

                                                     aaGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGcc                                                                                                                                                                                             >  1:18152/1‑251 (MQ=255)

                                                     aaGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCAAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGcc                                                                                                                                                                                             >  1:355915/1‑251 (MQ=255)

                                                       gTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGt                                                                                                                                                                                                                                     >  1:1435800/1‑209 (MQ=255)

                                                         gACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAcc                                                                                                                                                                                         >  1:495689/1‑251 (MQ=255)

                                                         gACCGACGGTGTTGTAACTGACGAAATTCACGACCTGTCGGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAcc                                                                                                                                                                                         <  2:1544242/251‑1 (MQ=255)

                                                              aaggggttgaAACTGACGAAATACACTAACTCTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTCGAAGACCTGGTAACTTGCCGTAGCAACGACGACTCCAGCTTGTTCAGCCGCGACCAGGGTGCCTACATGGACGTATCCCTCAAGAAGGTGCTCTCCGTCGGTTCGTACCTGATCCAGTTACGGGTACACGATTACGCCacacgcgga                                                                                                                                                                                    >  2:1725592/6‑243 (MQ=255)

                                                              aCGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGtt                                                                                                                                                                                                                <  2:2172925/223‑1 (MQ=255)

                                                                 gtgtTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATcc                                                                                                                                                                                                                                       <  2:1898929/197‑1 (MQ=255)

                                                                   gttgtAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGAt                                                                                                                                                                               <  1:159063/251‑1 (MQ=255)

                                                                       tAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTCCCTGGAACACGATGACGCCAACCGTGCATTGATGGGt                                                                                                                                                                           >  2:755159/1‑251 (MQ=255)

                                                                        aaCTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAAc                                                                                                                                                                                          >  1:262753/1‑235 (MQ=255)

                                                                        aaCTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTg                                                                                                                                                                          <  1:991834/251‑1 (MQ=255)

                                                                             aCGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAAc                                                                                                                                                                     >  1:396360/1‑251 (MQ=255)

                                                                                  aTTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTa                                                                                                                                                                                                                                          <  1:1347527/177‑1 (MQ=255)

                                                                                   ttCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCg                                                                                                                                                                                                                  <  1:84455/200‑1 (MQ=255)

                                                                                        tACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGATTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCa                                                                                                                                                          <  1:1579417/251‑1 (MQ=255)

                                                                                         aCCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGACGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGCAGAATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCAAACCGTGCATTGATGCGTGCGAACATGCAACGTCAg                                                                                                                                                         >  2:1579263/1‑251 (MQ=255)

                                                                                         aCCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTc                                                                                                                                                                                                               >  1:1899125/1‑197 (MQ=255)

                                                                                         aCCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAg                                                                                                                                                         >  2:327547/1‑251 (MQ=255)

                                                                                         aCCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAg                                                                                                                                                         >  1:2006309/1‑251 (MQ=255)

                                                                                                        aagaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACCCTg                                                                                                                                          >  2:2121484/1‑251 (MQ=255)

                                                                                                          taagaggcagaCGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcg                                                                                                                                        <  2:873791/241‑1 (MQ=255)

                                                                                                             ggCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATTGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCACCCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgggc                                                                                                                                     >  2:340804/1‑251 (MQ=255)

                                                                                                               gaccgactttATCGCCCAGGCGAACTCCAACGTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACGTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTg                                                                                                                                   <  2:2076979/243‑1 (MQ=255)

                                                                                                               catcTACGTTATCGCACAGGGCAACTCCAACTTGGATGAACAAGACCACTTCGTAAAAGACCTGGTTACTTTCCGTAGCAAAGGCTAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTTATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGTGTGCGAACATGCACCGTCAGGCCGTTCCGACTCTGCGCGCTg                                                                                                                                   <  2:1460806/248‑1 (MQ=255)

                                                                                                                 aCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATg                                                                                                                                                                              >  1:13960/1‑206 (MQ=255)

                                                                                                                    aCGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAg                                                                                                                              <  1:340834/251‑1 (MQ=255)

                                                                                                                    aCGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTAACCGTCTGTGCGTCCCTGATCCCGTTCCTGGAACAGGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGACCGTTCCGACTCTGCGCGCTGATAAg                                                                                                                              <  1:1629765/251‑1 (MQ=255)

                                                                                                                    aCGTTAGCGCCCAGACGAACTCCAACTTGGATGAAGAAGGCCACTTCGTATAAGACCTGGTAACTTTCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAg                                                                                                                              <  2:815264/251‑1 (MQ=255)

                                                                                                                     cGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTGGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGGGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGc                                                                                                                             <  2:396327/251‑1 (MQ=255)

                                                                                                                         aTCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCt                                                                                                                         >  2:613544/1‑251 (MQ=255)

                                                                                                                                aGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttggt                                                                                                                  >  1:184745/1‑251 (MQ=255)

                                                                                                                                  gCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc                                                                                                                >  1:655646/1‑251 (MQ=255)

                                                                                                                                          cAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGActc                                                                                                                                            >  1:1022441/1‑215 (MQ=255)

                                                                                                                                                ggATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCTTAGCAAAGGCGAATCCATCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTg                                                                                                  <  1:228038/251‑1 (MQ=255)

                                                                                                                                                    gaagaaGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATggg                                                                                                                                                                            >  1:1735791/1‑173 (MQ=255)

                                                                                                                                                          gacacgtcggtggaagaCCTTGGAACTTGCCGTGGCAAACGCGAAGCCACCGTGGTCAGCCCCGACCAGTTTGACTATATGGACGTATCCATCCACCATGTGTGTGACGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGCCGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGt                                                                                        <  2:1406114/239‑1 (MQ=255)

                                                                                                                                                                 tCGTAGAGGACCTGGTAACTTGCCGTAGCAAATGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTcc                                                                                 <  2:1045047/251‑1 (MQ=255)

                                                                                                                                                                 tCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTg                                                                                               <  1:30933/237‑1 (MQ=255)

                                                                                                                                                                   gtaacagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCATCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCTGTGCTTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCg                                                                                                                                                     <  2:1172904/176‑1 (MQ=255)

                                                                                                                                                                      gaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGgtgt                                                                            <  1:1308718/251‑1 (MQ=255)

                                                                                                                                                                      gaagaCCTGGTAACTTGCCGGAGCAAAGGCGAATCCAGCTTGGTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGgtgt                                                                            <  2:2006106/251‑1 (MQ=255)

                                                                                                                                                                         gaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAAc                                                                         <  1:1153212/251‑1 (MQ=255)

                                                                                                                                                                           ccTGCTAACTTGCCGTAGCAAAGGCTAATCCAGCTTGTTCAGCCGCGACCACGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTg                                                                                                                                   <  1:1474813/191‑1 (MQ=255)

                                                                                                                                                                              ggTTACTTGCGTTAGCAAAGGCGAATCATGCTTGTTCAGCCGCGCCCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTCTCCTTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGTCGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCTTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCgg                                                                    <  2:184732/251‑1 (MQ=255)

                                                                                                                                                                                  aCTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATACGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGc                                                                >  1:1138622/1‑251 (MQ=255)

                                                                                                                                                                                       ccGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGc                                                                                                                             >  1:1079058/1‑185 (MQ=255)

                                                                                                                                                                                        cGTAGGAAAGGCGTATCCAGCTTGTTCGTCCGCGCCCAGGTTGACTACATGGGCGTATCCAACCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACg                                                          <  2:2048781/251‑1 (MQ=255)

                                                                                                                                                                                        cGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACg                                                          <  2:1602080/251‑1 (MQ=255)

                                                                                                                                                                                        cGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACg                                                          <  1:2212663/251‑1 (MQ=255)

                                                                                                                                                                                        cGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACg                                                          <  1:2166843/251‑1 (MQ=255)

                                                                                                                                                                                         gTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgt                                                         >  1:2153117/1‑251 (MQ=255)

                                                                                                                                                                                         gTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgt                                                         >  1:941214/1‑251 (MQ=255)

                                                                                                                                                                                         gTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgt                                                         <  1:726721/251‑1 (MQ=255)

                                                                                                                                                                                           aGCAAAGGCGAATCCAGCTTGTTCGGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtgg                                                       <  2:355885/251‑1 (MQ=255)

                                                                                                                                                                                           aGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtgg                                                       >  2:1153124/1‑251 (MQ=255)

                                                                                                                                                                                           aGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtgg                                                       >  2:289081/1‑251 (MQ=255)

                                                                                                                                                                                           aGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACgtgg                                                       <  1:1696761/251‑1 (MQ=255)

                                                                                                                                                                                           aGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCAATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGAATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGTGCGCTGATAAGc                                                                                                                             >  1:1172993/1‑181 (MQ=255)

                                                                                                                                                                                                 ggCGAATCCATCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACa                                                                                                                                                                    <  2:567825/136‑1 (MQ=255)

                                                                                                                                                                                                   cGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGGGTCg                                               >  1:624394/1‑251 (MQ=255)

                                                                                                                                                                                                      tcccAGCTTGTTCAGCCGCTACCAGGTTTACTACATTGACTTATCCATCAAGCTGGTGGTATCCGTCGGTGCGTCCCTTATCCCGTTCCTGGAACACGATGGCGCCAACCGTGCATTGATTGGTGCGAACATGCAACGTCATTCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTTCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGGTc                                            <  2:2162801/249‑1 (MQ=255)

                                                                                                                                                                                                      aTCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACg                                                          >  1:54589/1‑237 (MQ=255)

                                                                                                                                                                                                      aTCCAGCTGGGTCAGCCGCTACCAGGTTGACTACATGTCCGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCGGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGAGGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTc                                            <  2:38845/251‑1 (MQ=255)

                                                                                                                                                                                                               tttCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTgg                                   <  2:2295826/250‑1 (MQ=255)

                                                                                                                                                                                                                 tCAGCCGCGACCAAGTTGACTACATTGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACAAGATGACGCGAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGACGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGAt                                 <  2:2106131/251‑1 (MQ=255)

                                                                                                                                                                                                                         gACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCg                         >  1:2077413/1‑251 (MQ=255)

                                                                                                                                                                                                                             aGGTTGACTACATGGACATATCCATCCAGCAGGTGGTTTCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATc                     <  1:570845/251‑1 (MQ=255)

                                                                                                                                                                                                                              ggTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTc                                                                                  <  1:1208516/189‑1 (MQ=255)

                                                                                                                                                                                                                                      aCATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAg                                                                                                                              <  2:928023/137‑1 (MQ=255)

                                                                                                                                                                                                                                      aCATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTAt                                                                                                          <  2:1115171/157‑1 (MQ=255)

                                                                                                                                                                                                                                        aTGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGt          <  1:2121698/251‑1 (MQ=255)

                                                                                                                                                                                                                                              gTATCCACCCAGCAGGTGGTATCCTTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGa    <  1:1519149/251‑1 (MQ=255)

                                                                                                                                                                                                                                                atccatccAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAAAGTGGTGGCGTCGTTCAGTTCGTGGATGCTTCCCGTATCGTTTTCCAAGTTAACGAAg  >  2:1915711/1‑251 (MQ=255)

                                                                                                                                                                                                                                                atccatccAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCCCGCTGATACGCCGCTGGTTGGTACTGGCATGGCACGTGCTGTTGCCGTTGACTCCGGTGTACCTGCGGGCGCTTAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTCTCGTTTTCAAAGTTAACGAAg  >  2:526424/1‑251 (MQ=255)

GTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAG  >  NC_000913/4182969‑4183459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`