Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,809 G→T G1189C (GGT→TGT)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,8090GT97.7% 446.0 / NA 135G1189C (GGT→TGT) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (59/70);  total (64/71)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

TATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTT  >  NC_000913/4184562‑4185053
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
aaTGAACATCGATCAGATCCTCGAAACCCCCGTGGGTATTGCTACGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACtgtg                                                                                                                                                                                                                                                   <  1:615679/250‑1 (MQ=255)
    aaCATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGaa                                                                                                                                                                                                                                               >  2:94621/1‑251 (MQ=255)
         cGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACATGCGCAAAGGTATGCCAATCGCCAGGCCGGTGTTCGACTGTGCGAaagaag                                                                                                                                                                                                                                          >  2:639968/1‑251 (MQ=255)
         cGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGAGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGTAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGGGCgaaggaag                                                                                                                                                                                                                                          >  2:615830/1‑251 (MQ=255)
         cGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCACCAAGACGTCGCGAAACTGCGCGCATTCATGCAGCGTGCGTACGACCTGGGCGCTGACGTTCGCCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATCCGTCTGGCGGAAAACCTGCGCAAAGGTATGCAAATCGCAACGCCGGTGTTCGACAGGGCGAaagaag                                                                                                                                                                                                                                          >  2:830580/1‑251 (MQ=255)
          ggTCAAATCCTCGAAACCCCCCTGGGTATGGCTGCGAAAGGTATCGGCGAAAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGCCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGc                                                                                                                                                                                                                                         <  2:755244/251‑1 (MQ=255)
           gTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAaagaagaa                                                                                                                                                                                                                                        >  2:706789/1‑249 (MQ=255)
                  ccTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGTGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCC‑GTGTTCGACTGTGCGAAAGAAGCAGAAATTaa                                                                                                                                                                                                                                >  1:935484/1‑251 (MQ=255)
                      gAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAaga                                                                                                                                                                                                                             >  2:586984/1‑251 (MQ=255)
                         aCCCAACTTGGTATGGCTGCGAAAGGTATCGGCGACAAGTACAACGCCCAGCTGAAACAGCAGCAAGGAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGGTCGTCAGAAAGTTGACCTTAGTACCTTCAGCGATGAAGAATTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGGCAATCGCCACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAgct                                                                                                                                                                                                                          <  2:1255832/251‑1 (MQ=255)
                            cACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAgctgct                                                                                                                                                                                                                       <  1:650477/251‑1 (MQ=255)
                             aCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGctgctg                                                                                                                                                                                                                      >  1:1221590/1‑251 (MQ=255)
                                      tGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGc                                                                                                                                                                                                             <  1:813980/251‑1 (MQ=255)
                                      tGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGc                                                                                                                                                                                                             <  1:1208765/251‑1 (MQ=255)
                                          tGCGAGAGGTATCGGCGACAAGCTCAACGCCATGCTGAACCAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGAcc                                                                                                                                                                                                         <  1:748987/251‑1 (MQ=255)
                                          tGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGAcc                                                                                                                                                                                                         >  2:1209009/1‑251 (MQ=255)
                                               aaGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGTGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAACCTTGGCGACCTGCCg                                                                                                                                                                                                    >  1:315632/1‑251 (MQ=255)
                                               aaGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAAGTAAAGGCCTGCTGAACCTTTGCTACCTGCCg                                                                                                                                                                                                    >  2:1303125/1‑251 (MQ=255)
                                               aaGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATAGCAACGCCGGTGTTCGACTGTGCGAAAGAAGAAGAAATTAAAGAGCGGCGGAAAATTGGGGACCTGCCg                                                                                                                                                                                                    >  2:1260308/1‑251 (MQ=255)
                                                aGGTATCGGCGACAAGATCAACGCCATGCTGAAACCGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGGGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGa                                                                                                                                                                                                   <  1:1033451/251‑1 (MQ=255)
                                                 ggTATCGGCGACAATATCAATGCCATGCGGAAACAGCAGCAAGCAGTCGCGAAACTGCGCAGATTCATCCGGCGGGCGTACGTTAGGGGCGCTGACGTTCGTCAGAAAGTTGACCTGTGTACCTTCAGCGTTGAAGAAGCTATGCGTCTGGCTGAAATCCTGCGCAAAGGTATGCCAATCGCAACGTCGTTGTTCGACTGTGCGAAAGAAGCACAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGAc                                                                                                                                                                                                  <  2:863249/251‑1 (MQ=255)
                                                     tCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTcc                                                                                                                                                                                              >  1:1276503/1‑251 (MQ=255)
                                                      cGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCg                                                                                                                                                                                             >  2:814156/1‑251 (MQ=255)
                                                         cGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTc                                                                                                                                                                                          >  1:188551/1‑251 (MQ=255)
                                                         cGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTc                                                                                                                                                                                          >  1:1083898/1‑251 (MQ=255)
                                                         cGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCAAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTc                                                                                                                                                                                          >  2:460226/1‑251 (MQ=255)
                                                         cGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTAGGCCAATCGCAACGCCGGTGTTCGACTGGGCGAAAGAAGCAGAAATTAAAGAGCGGCTGAAACTTGGCGACCTGCCGACTTCCGGTc                                                                                                                                                                                          >  2:1029285/1‑251 (MQ=255)
                                                         cGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGACAGAAAGTTGACCTGAGTACCTTCAGCGATGAGGAAGTGATGCGTCTGGCTGAAAACCTGCGAAAAGGCATGCCAACCGCAACGCCGGTGGTCGACTGTGCGAAAGAGGCAGCAAATAAAGAGCTGCTGAAACTTGGCGCACTGCCGACGTCCGGTc                                                                                                                                                                                          >  2:1278488/1‑251 (MQ=255)
                                                         cGACAAGATCAACGCCATGCTGAAACAGCAACAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTc                                                                                                                                                                                          >  1:297135/1‑251 (MQ=255)
                                                          gACAAGATCAACGCCAGGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCa                                                                                                                                                                                         <  2:897013/251‑1 (MQ=255)
                                                                  cAAGGCCAAGCTGAAACAGCAGCAAGAAGTCGCGAACCTGCGCGAATTCGTCCGGGTTGCGTACGTACTGGTGGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGAGGAAGGAGTTATGCGTCTGGCTGAAAACCTGCGCGAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGcc                                                                                                                                                                                 <  2:476/251‑1 (MQ=255)
                                                                  cAACGCCATGCTGAAACAGCAGCCAGAAGGCGCGAAACTGCGCGGGTGCATCCAGCGTGCGTACGTGCTGGACGCTCACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAATACCTGCGCAAAGGTAGGCCAATCGCAACGCCGGTGTTAGACTGTGCGAAAGAAGCAAAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGcc                                                                                                                                                                                 <  2:144333/251‑1 (MQ=255)
                                                                    aCGCCATGCTTAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAACGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCTCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTg                                                                                                                                                                               <  1:1260054/251‑1 (MQ=255)
                                                                          tGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGAt                                                                                                                                                                         <  1:1285673/251‑1 (MQ=255)
                                                                          tGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGAt                                                                                                                                                                         >  1:885257/1‑251 (MQ=255)
                                                                           gCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCg                                                                                                                                                                                                    <  2:1229853/223‑1 (MQ=255)
                                                                                     cagcaAAAAGTCGCGAAACTGCGCGAATTAATCCGGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGGGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTgg                                                                                                                                                              <  2:501861/251‑1 (MQ=255)
                                                                                       gcaAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGCAAGAAGCAGAAACTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGAAGGTCGCACTGGtc                                                                                                                                                            >  2:933932/1‑250 (MQ=255)
                                                                                       gcaAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTAACTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTAAGATCCGCCTGTACGATGGTCGCACTGGTg                                                                                                                                                            >  2:714309/1‑251 (MQ=255)
                                                                                            gagtgtcgAAACTGCGCGAATTCATCCGTCGTGCGTACGGTCTGTGCGATGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGGCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGGAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAAATCCGCCTGTACGATGGTCGCACTGGTGAACAg                                                                                                                                                       <  2:297209/245‑1 (MQ=255)
                                                                                            aagTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGCGCATCTGAACCTTGGCGACCTGACGACTTCCGGTCAGATCCGCCTGCACGATGGGCGCCTTGGTGACCAg                                                                                                                                                       >  2:10105/1‑251 (MQ=255)
                                                                                                  ggAAACTCCGCGGATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCTTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTGAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAg                                                                                                                                                 <  2:315706/250‑1 (MQ=255)
                                                                                                  cgAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAag                                                                                                                                                                                                                              >  1:1096856/1‑174 (MQ=255)
                                                                                                  cgAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAag                                                                                                                                                                                                                              >  2:1216291/1‑174 (MQ=255)
                                                                                                  cgAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGAACCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAg                                                                                                                                                 >  2:1141816/1‑251 (MQ=255)
                                                                                                  cgAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGAGCTGACGTTCGTCAGAAAGTTGACTTGAGTACCTTCTGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCACAGGGATGCCAATCGCAACGCCGGTGTTCGACTGCGCGAAAGAAGCAGAAATTAAAGAGATGCTGAAACTGGGCGACTTGCCGACGTCAGCTCAGATGCGCCTGTACGATGGTCGCACTGGTGAACAGTGCGAg                                                                                                                                                 >  1:822006/1‑251 (MQ=255)
                                                                                                     aaCTGCGCGAATTCAGCCAGGGTGCGGACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGt                                                                                                                                              <  2:922384/251‑1 (MQ=255)
                                                                                                               aTTCATCCAGCGTGCGTACGATGTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGGACCTTCAGCGATGAAGAAGTTATGGGTCTGGCTGAAAGCCGGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCGGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCg                                                                                                                                    <  2:365018/251‑1 (MQ=255)
                                                                                                                        gcgtgcgtACGATCTGGGCGCTGACGTGCGTCAGTAAGTTTACCTGAGTACCTGCAGCGATAAAGAAGTTATGCGTCTGGCTGAAGACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtaca                                                                                                                           <  1:933736/251‑1 (MQ=255)
                                                                                                                         cgtgagtACGATCTGGGCGCTAGCGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAGAGTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacat                                                                                                                          <  2:196405/251‑1 (MQ=255)
                                                                                                                            gcgtACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGGACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacatgta                                                                                                                       <  1:1402930/251‑1 (MQ=255)
                                                                                                                             cgtACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTtacatgtac                                                                                                                      <  1:1326121/251‑1 (MQ=255)
                                                                                                                                 cGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGc                                                                                                                                                                                                                                         <  2:550268/132‑1 (MQ=255)
                                                                                                                                   aTCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGcc                                                                                                                                                                                 <  1:155886/186‑1 (MQ=255)
                                                                                                                                   aTCTCGGCGCTGCCAGTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTCTGCGTCTGTCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTg                                                                                                                <  2:83797/251‑1 (MQ=255)
                                                                                                                                      tGGGCGCGGGCGTTCGTCAGAAAGTTTACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAATACCTGAGCGAAGGTATGCGAGTCGCAACGCCGGTGGTCGACTGGGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGaaa                                                                                                             <  2:534494/251‑1 (MQ=255)
                                                                                                                                        ggCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGCTTGCATGTACCTGCTGACACt                                                                                                           >  2:278956/1‑251 (MQ=255)
                                                                                                                                           gtgtccgttcaagaaaaggTTCACATGAGTAACTTATACGTTGAATAAGTTATGCGTCTGGTTGAAAACATGCGCAAAGGTATGCCAATCTCAACGCCGGTGTTACACTATGCGAAAGAAGCGGAAATTAGAGAGATACTGAACCTTGTCGACCAGCCGACTTGCGGTCAGATCCGCCTGTACGATGTTCGCACTGGTGAACAGTTCGGGCGTCCGATAACCGTTGGTTACATGTACATGCTGAAACTGaa                                                                                                        <  1:1299801/233‑1 (MQ=255)
                                                                                                                                           gcTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGaa                                                                                                        <  1:706629/251‑1 (MQ=255)
                                                                                                                                                    cGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGt                                                                                               >  2:920436/1‑251 (MQ=255)
                                                                                                                                                    cGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGt                                                                                               <  1:8462/251‑1 (MQ=255)
                                                                                                                                                         gAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAATGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACt                                                                                                                                                                                                                 >  1:550145/1‑132 (MQ=255)
                                                                                                                                                          aaaGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTcgacga                                                                                         <  2:885442/251‑1 (MQ=255)
                                                                                                                                                          aaaGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTcgacga                                                                                         >  2:144685/1‑251 (MQ=255)
                                                                                                                                                           aaGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGTGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACAGTGCGAACGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACTTGCTGAAACTGAACACCCTGGTTgatgac                                                                                        >  1:268515/1‑251 (MQ=255)
                                                                                                                                                           aaGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAAc                                                                                                                                                         >  2:155943/1‑186 (MQ=255)
                                                                                                                                                                 gccTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTTAAACTTGGCGACCTGCCAACTTCCGGTCAGATCCACCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATg                                                                                  <  1:204689/250‑1 (MQ=255)
                                                                                                                                                                 agcagagaACCTTAAGAGATGAAGAAGGGAGGCGGCTGGCTGAAAACCTGCGCAAAGGTATGCCAAGCGCAACGCCAGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGGGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATg                                                                                  <  2:268584/247‑1 (MQ=255)
                                                                                                                                                                 aCCTGGGTACCTTCAGGGATGAAGAAGTTATGCGTCTGGCTGAAAACCGGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATg                                                                                  <  2:1343562/251‑1 (MQ=255)
                                                                                                                                                                 aCCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATg                                                                                  <  1:94564/251‑1 (MQ=255)
                                                                                                                                                                 aCCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATg                                                                                  <  1:144627/251‑1 (MQ=255)
                                                                                                                                                                 aCCTGAGTACCTTCAACGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCACCGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATg                                                                                  <  1:555276/251‑1 (MQ=255)
                                                                                                                                                                 aCCTGAGTACCTTAAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCCCAAAGGTATGCCAATCGCAACGCCGGTGTTCACCTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGGCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATg                                                                                  <  2:482031/251‑1 (MQ=255)
                                                                                                                                                                    tgcgTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCAc                                                                               <  2:1084135/248‑1 (MQ=255)
                                                                                                                                                                          ccTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACt                                                                                                           <  2:265968/217‑1 (MQ=255)
                                                                                                                                                                              cAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCa                                                                     <  2:1276776/251‑1 (MQ=255)
                                                                                                                                                                              cAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCGGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCa                                                                     <  1:714143/251‑1 (MQ=255)
                                                                                                                                                                              cAGCAATGAAGAAGTTCTGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTTGACGACAAGATGCACGCGCGTTCCa                                                                     <  1:1221765/251‑1 (MQ=255)
                                                                                                                                                                                                 cggcTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGGACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGt                                                  <  1:308605/248‑1 (MQ=255)
                                                                                                                                                                                                 cGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAAc                                                                                                                                      >  2:628098/1‑167 (MQ=255)
                                                                                                                                                                                                 cGTCCGGCTGAAAACCTGCGCAAAGGTATGCCAACCGCAACGCCGGAGGTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAATTTTGCGACCTGCCGACTTCGGGCCAGATCCGCTTGTACGATGGTAGCACTGGAGAACAGTGCGAACGTCCGCTAACCGTTGGTTACATCTAAATGCCGAAACTGCACCACCTGGCAGCAGACAAGACACAGGCGCGTTCACCCGGATACTACATCCCGGt                                                  >  2:1120804/1‑251 (MQ=255)
                                                                                                                                                                                                  gTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGAt                                                                                   >  1:265899/1‑217 (MQ=255)
                                                                                                                                                                                                      ggCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTc                                             <  1:701817/251‑1 (MQ=255)
                                                                                                                                                                                                      ggCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTc                                             <  1:1029062/251‑1 (MQ=255)
                                                                                                                                                                                                           tgcatcagaggccggttATGCCAATCGCAACGCCGTGGTTCGACTGTGCTAAAGAAGCAGAAATTAAGGAGCTGCTGAACCTTGGCGACCTGCCGACTTCCGGTCAGTTCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTcagcag                                        <  1:626356/235‑1 (MQ=255)
                                                                                                                                                                                                                cTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCt                                   <  2:951128/251‑1 (MQ=255)
                                                                                                                                                                                                                cTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCt                                   >  2:180378/1‑251 (MQ=255)
                                                                                                                                                                                                                cTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCt                                   <  1:460119/251‑1 (MQ=255)
                                                                                                                                                                                                                  gcgcAAAGGTATCCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCt                                   <  1:780299/249‑1 (MQ=255)
                                                                                                                                                                                                                  gcgcAAAGATAGGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGTCCTGCCGACTTcc                                                                                                                                                                                              <  1:925720/94‑1 (MQ=255)
                                                                                                                                                                                                                   cgcAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGCTGCATGGGCGTTCACCCGGTTCTTAACGCATGGTTACTCAGCAGCCCCTggg                                >  2:1346751/1‑251 (MQ=255)
                                                                                                                                                                                                                   cgcAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTggg                                >  2:617121/1‑251 (MQ=255)
                                                                                                                                                                                                                   cgcAAAGGTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCACCAGCCGCTggg                                >  2:865864/1‑251 (MQ=255)
                                                                                                                                                                                                                         ggTATGCCAATCGCAACGCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTcagcag                                        <  1:1220424/237‑1 (MQ=255)
                                                                                                                                                                                                                                          gCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATACGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTCCATGTACAAGCTGAAACTGAAACACCTGGCCGAAGACAAGCTGTAAGTGCGCACCACCGGTTCTTACAGCCTGGTTACTAAGCAGCCGCCGGGTGGTAAGGGCCAGTCAGGTGGTc         >  2:408989/1‑251 (MQ=255)
                                                                                                                                                                                                                                          gCCGGTGTTCGACTGTGCGAAAGAAGCAGAAATCAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCATGTACGATGGt                                                                                                                                                                      >  2:925907/1‑94 (MQ=255)
                                                                                                                                                                                                                                              gtgtTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTcgacga                                                                                         <  1:277068/167‑1 (MQ=255)
                                                                                                                                                                                                                                              gtgtTCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATg                                                                                  <  1:1010996/174‑1 (MQ=255)
                                                                                                                                                                                                                                              gtgtTCGACTGTGCGAAAGAAGCAGAAATGAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGTTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTg                                                                                                                <  2:4009/144‑1 (MQ=255)
                                                                                                                                                                                                                                                 ttCGACTGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTccggtctcttataca  >  2:1220668/1‑237 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
TATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAAGGTATGCCAATCGCAACGCCGGTGTTCGACGGTGCGAAAGAAGCAGAAATTAAAGAGCTGCTGAAACTTGGCGACCTGCCGACTTCCGGTCAGATCCGCCTGTACGATGGTCGCACTGGTGAACAGTTCGAGCGTCCGGTAACCGTTGGTTACATGTACATGCTGAAACTGAACCACCTGGTCGACGACAAGATGCACGCGCGTTCCACCGGTTCTTACAGCCTGGTTACTCAGCAGCCGCTGGGTGGTAAGGCACAGTTCGGTGGTCAGCGTTT  >  NC_000913/4184562‑4185053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑