Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,815,810 Δ1 bp intergenic (‑42/+24) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,8090G.96.2% 393.8 / 15.3 107intergenic (‑41/+25)pyrE/rphorotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base . (54/46);  minor base A (3/1);  total (58/49)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.74e-01

Only 100 of 112 total aligned reads displayed.

AAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTC  >  NC_000913/3815565‑3816056
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       
aaaCAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGCTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAAAAAACTGGCGCTGATAAGGTTTCATCCCTTCGCTCCCTATCTTACTTTTCTACAGACAAAAAAAAgcaaccc                                                                                                                                                                                                                                                   >  1:294651/1‑245 (MQ=255)
        gATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTACAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAAATCGACAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAACCTGGCGCTGATATGGTTTCATGCAGTCGCTCGTCAACTTACTTATCCTCAGAAAAAAAAAAGCGAATCATCAGTCg                                                                                                                                                                                                                                          >  2:932434/1‑251 (MQ=255)
        gATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGGAAACTCGCCAAACTTTAACACCTGCCTGCTAAGCGCAAATTCAATAAACTGGCACTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACCCTTCCGTCg                                                                                                                                                                                                                                          >  2:379169/1‑251 (MQ=255)
         aTCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCACACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACCGACAAAAAAAAGCGACTCATCAGTCGc                                                                                                                                                                                                                                         >  1:2428/1‑251 (MQ=255)
         aTCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGc                                                                                                                                                                                                                                         >  1:123806/1‑251 (MQ=255)
         aTCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACCCATAAGTCGc                                                                                                                                                                                                                                         >  2:326675/1‑251 (MQ=255)
         aTCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTAAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCCCATCTTACTTTTCTACAGACAAAAAAAAGAGACTAATCAGTCGc                                                                                                                                                                                                                                         >  2:810678/1‑251 (MQ=255)
         aTCGAACTAAATGCCGGAATCCACTAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTCGCGCCCGTATCTAAGCGTAAACTCGCCAAACTTTAACACTTGCATGCTAAGCGCAAATCCAATAAACCGGTGTTGTTCTGGTTCCATGCCTTCGCCCAACATCTTAATTTACTACAGAGAAAAAAAAgctattcagaagtccg                                                                                                                                                                                                                                          >  2:326201/1‑236 (MQ=255)
          tCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGCCAAAAAAAAGCGACTCATCAGTCGcc                                                                                                                                                                                                                                        >  1:732270/1‑251 (MQ=255)
                  gcgcggaggtgttaataagagacaggCGTAAAAACGGCCTAACAGTGCCAGAGCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCATTCGCCTTAAAAAt                                                                                                                                                                                                                                <  1:182509/226‑1 (MQ=255)
                    tggctgAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCa                                                                                                                                                                                                                              <  1:810524/246‑1 (MQ=255)
                           aTCCACCCACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCCAACCTTAACACCTGCTTGCTAAGCGCAAATTCAAAAAACTGGCGCTGATATGGTTTACTGCCTTCGCCCCCCATATTACTGTTCTACCACCAAACAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGcc                                                                                                                                                                                                                       >  2:502642/1‑251 (MQ=255)
                           aTCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGcc                                                                                                                                                                                                                       >  1:860635/1‑251 (MQ=255)
                                ccaACGCTTCAGCGTAAAAACGGCCCAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATCCAATAAACTGGCGCTGATATGGATTCATGCCTTCGCTCCTCATCTTACTTTTCAACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCAAgcgc                                                                                                                                                                                                                  >  1:945314/1‑251 (MQ=255)
                                  aaCGCTTCAGCGAAAAAACGGCCTACCAGGGCCAGGTCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAGACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgccg                                                                                                                                                                                                                <  2:934621/251‑1 (MQ=255)
                                     gCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATCTGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAggcggcacc                                                                                                                                                                                                             <  2:57272/251‑9 (MQ=255)
                                      cTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCACGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTCCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAACGACTCCTCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCtt                                                                                                                                                                                                            >  1:546748/1‑251 (MQ=255)
                                                   aCGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGAATCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCCCCTTCCGCGTCGCTACa                                                                                                                                                                                               >  2:1059674/1‑251 (MQ=255)
                                                      gCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCCGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGACTTCTGCGTCGCTACAATg                                                                                                                                                                                            >  1:45104/1‑251 (MQ=255)
                                                            cAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcg                                                                                                                                                                                      <  1:1059481/251‑1 (MQ=255)
                                                              ctGCCAGTCCGCCCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgat                                                                                                                                                                                    <  2:218597/250‑1 (MQ=255)
                                                                   aGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACAACTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGCTTCATGCCTTCGCTCCTCCTCTTACTTTTCTACAGACAAAACAAAGCGACCTATCAGTCGCCTTAAAAATCAGTTTGCCAGCGGCGCCTTCTGCTTctgtcttataaccacactacgcc                                                                                                                                                                               >  2:182565/1‑229 (MQ=255)
                                                                    gatctggcCCGGTAATAAACAACCCGGCGTTCGAGAAAGTGGGGATGTTTCGCCCGGATTTCCACGTAAGCTCGACAAAATTTCACACCTTCTTGCTAAGCGCAAATTCAATACACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCTACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCt                                                                                                                                                                              <  2:590772/245‑1 (MQ=255)
                                                                      tCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCg                                                                                                                                                                            <  2:198986/251‑1 (MQ=255)
                                                                         cgcCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGc                                                                                                                                                                         <  1:245421/251‑1 (MQ=255)
                                                                         cccccGCTATTAAACGGCCCGGCGTTGAAGTAATAGTGGCTTTTTCGCCCGGATTTCAGCCTAAACTCGCCACACTTTAACCCCTCCTTGCTAATCGGCAATTCATTAAACTGGCGCTGATATGGTTTCTTGCCTTCGCTCCTCATCTTACTTTTCTACATACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGc                                                                                                                                                                         <  2:945493/249‑1 (MQ=255)
                                                                              ggTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCagag                                                                                                                                                                    <  1:337379/251‑1 (MQ=255)
                                                                                 atcagaCAGCCCGACGTTGAACAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTCCTAAGCGCTAATTCAATAAACTGGCGCTGATATAGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCca                                                                                                                                                                 <  1:326122/246‑1 (MQ=255)
                                                                                 aTTGAACGGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAATCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAGTCCCCTCGGGCCAGAGCca                                                                                                                                                                 <  1:501465/251‑1 (MQ=255)
                                                                                 aTTAATCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCca                                                                                                                                                                 <  1:481348/251‑1 (MQ=255)
                                                                                 aTTAAACGGCCCAGCGTTGAATAAATAGTGGCTTTTTCTCCCGGATTTCAGCTTAAACTCGCCAAACGTTAACCCCTGCTTGCTAAGCGCAAATTCAATAAGCGGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGTCAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCca                                                                                                                                                                 <  1:467721/251‑1 (MQ=255)
                                                                                 aTTAAACAGCCCGGCGTTGAAGAAATGGGGGATTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATTCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCca                                                                                                                                                                 <  2:378145/251‑1 (MQ=255)
                                                                                 aTTAAACAGCCCGGCGTTGAAGAAATAGGGGCTGTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCca                                                                                                                                                                 <  1:326596/251‑1 (MQ=255)
                                                                                      aCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACCCCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCAAGAGCCAACAAg                                                                                                                                                            >  1:668745/1‑251 (MQ=255)
                                                                                      aCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAg                                                                                                                                                            >  1:959527/1‑251 (MQ=255)
                                                                                      aCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTAATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAATCCTCTCGGGCCAGTGTCAACAAg                                                                                                                                                            >  1:552301/1‑251 (MQ=255)
                                                                                      aCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCCGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGCGCCAACAAg                                                                                                                                                            >  2:460683/1‑251 (MQ=255)
                                                                                      aCAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCAGGATTTCAGCGTAAACTCGACAAACTTTAACAACTGCTTGCTATGTGCTAATTCAATAAACTGGCGCTGATACGTTTTCAAGCTATAGCTCCTCCTCTTCCTTTTCTTCAGGCAAAAACAACCGCATTATTCGCTGCCTCAAAAATCAGTTTGCCAGTGGCGACTTCAGCGTCCCTACACTGGTATCGACTCCCCTctgtctagtacccacacg                                                                                                                                                            >  2:30571/1‑234 (MQ=255)
                                                                                        aGCCCGGCGTTGAAGAAATAGGGGCTTTTCCGCCCGGATTTCAGCGTAATCTCGCCAAACTTTCACACCTACTCGCTAAGCGCAAATTCATTAAACTTTCGCTGAATTGATTTCATGCCTTTGCTCTTCATCTTACTTTTCAACAGACATAAAAAAGTGACTCATCAGTCGCCTTTCAATTATACTCGCCAGAGCCTCCTTCTGCGACGCTATAATGGATTCGAGTTCCCTCGGGCCAGCGCCAACAAGAt                                                                                                                                                          >  2:40812/1‑251 (MQ=255)
                                                                                            cGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgat                                                                                                                                                                                    >  1:146634/1‑221 (MQ=255)
                                                                                                 ttGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCt                                                                                                                                                                              >  1:841179/1‑222 (MQ=255)
                                                                                                       aaaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAGAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                                                                                                           >  1:229003/1‑251 (MQ=255)
                                                                                                       aaaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                                                                                                           >  1:478791/1‑251 (MQ=255)
                                                                                                       aaaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                                                                                                           >  1:248258/1‑251 (MQ=255)
                                                                                                       aaaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCCCttactg                                                                                                                                           >  2:986925/1‑247 (MQ=255)
                                                                                                       aaaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCCCTTCATg                                                                                                                                           >  1:990483/1‑251 (MQ=255)
                                                                                                       aaaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCCGGGCCAGAGCAAAAAAGATGAGTAGCTCTTCATg                                                                                                                                           >  2:654911/1‑251 (MQ=255)
                                                                                                       aaaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTAAAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGGGTAGGCCTTAATg                                                                                                                                           >  2:481453/1‑251 (MQ=255)
                                                                                                       aaaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTAATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCATTCTGCGTCGCTACAATGGATTCGATTCCACTCGGGACAGAGCCAACAAGATGAGTAGCTCTTCATg                                                                                                                                           >  1:132816/1‑251 (MQ=255)
                                                                                                       aaaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCCACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTCCAATGGATGCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGGAGCTCTTCacg                                                                                                                                           >  2:927408/1‑249 (MQ=255)
                                                                                                       aaaTAGGGGCTTTTGCGCCCGGATCTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGTGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTCCATg                                                                                                                                           >  1:297921/1‑251 (MQ=255)
                                                                                                         aTAGGGGCTTTTGCGTCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATACACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCCACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATggg                                                                                                                                         >  1:359903/1‑251 (MQ=255)
                                                                                                         aTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGAAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATggg                                                                                                                                         <  2:478896/251‑1 (MQ=255)
                                                                                                                cTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACgg                                                                                                                                  <  1:87368/251‑1 (MQ=255)
                                                                                                                cTTTTGCACCCGGCTGTAAGCGAAAACTCGCAAACCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACATACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACgg                                                                                                                                  <  1:531581/251‑1 (MQ=255)
                                                                                                                 ttttGCGCCCGGATTTCAGTGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTAGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCTGCCTTCTGCGTCTCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGGGacctgc                                                                                                                                 >  1:123834/1‑246 (MQ=255)
                                                                                                                 ttttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGATTCATGCCTTCGCCCCTCATCTTACTTTTCTACAGCCAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTTCCAGCGACGCCCTCTGCGGCGCTACAATGGATTCGACTCACATCGGGCCACAGCCAACAATATGAGTAGCTCTTCATCGGTGAACGGc                                                                                                                                 >  2:984983/1‑251 (MQ=255)
                                                                                                                   ttGCGCCCGGATTTCAACGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTc                                                                                                                               <  1:653351/251‑1 (MQ=255)
                                                                                                                     ccacccGAATTTCAGCGTAAACTCACCAAACTTTAACACCTGCTTGCTAAGCACAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGc                                                                                                                             <  1:382506/248‑1 (MQ=255)
                                                                                                                        gcctgATTACAGCATAAACTCACAAAACTTTAACACCATCTTGCTAAGCGCAAATTGAATAAACTGACGCTGATATTGTTTAAGTCCATCCCTCCTCATCTTACTTGTATACAGACAAAAAAAAGCGACTCATCATTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCTATTCCCCTCGGGCCGGAGCCAACAAGATGGGTAGCTCTTCATGGGTGAACGGCTCGCCtt                                                                                                                          <  2:552409/247‑1 (MQ=255)
                                                                                                                        cccGGATTTCAGCTGAAACTCGGCATACTTTAACACCTGCTTGCTGAGCGAGAGTTCAATGAGCTGGTGCTGATATGGTTCCATGCCTTCGCTACTCATCTCACTAGTATATAGACAAAAAAAAGCGTCTCAGCGGTGGGCTTAAAAATAGGTTAGCCCGCGGCGCCCACTGCGTCGCGACAATGGATTCGATTCCCCTCGGGCCGGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGTCTCGCCtt                                                                                                                          <  2:752211/251‑1 (MQ=255)
                                                                                                                        cccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAGAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                                                                                                                          <  2:229064/251‑1 (MQ=255)
                                                                                                                        cccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                                                                                                                          <  1:131388/251‑1 (MQ=255)
                                                                                                                        cccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                                                                                                                          <  1:379089/251‑1 (MQ=255)
                                                                                                                        cccGGAGTTCAGGGTAAACTCGCAAAACTGTACCACCTGCTTGTTAACCGCAAATTCAAGAACTTGGCGCTGTTATGGTTTCATGCCTCCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAATCGACGCATCAGACGCCTTAAAAATCCGTTTGCCAGCGCCGCCTTCTGCGTCGCGACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                                                                                                                          <  2:82152/251‑1 (MQ=255)
                                                                                                                           ggATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCctgctg                                                                                                                       >  2:325483/1‑251 (MQ=255)
                                                                                                                           ggATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAAGGCTGGCATTCTg                                                                                                                       >  1:244189/1‑251 (MQ=255)
                                                                                                                           ggATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGACGCCTTCTGCGTCGCTACAATGGAGTCGATTCCCCTCGGGCCAGAGCCAACAAGATGATTAGCTCTTAATGGGTGACCGGGTTCCCTTCtt                                                                                                                       >  2:1023893/1‑250 (MQ=255)
                                                                                                                                    cGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCt                                                                                                              >  1:406317/1‑251 (MQ=255)
                                                                                                                                    cGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAAAAAGATGAGTAGCCCTTCATGGGTGAACGGGTCGCCTTCCGCCGTccccg                                                                                                              >  2:900811/1‑250 (MQ=255)
                                                                                                                                     gTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                                                             >  1:578350/1‑251 (MQ=255)
                                                                                                                                              cccaaCTTTAACCCCTGCTTGCTAAGCCCAAATTCAATAAACTTGCGCTGATATGTTTTCATGCCTTCGCTCCTCCTCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCGCTTCAat                                                                                                    <  2:668868/248‑1 (MQ=255)
                                                                                                                                               cAAACTTTAACACCTGCTTGCTAAGCGCAAAGTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTGTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatg                                                                                                   <  1:882169/251‑1 (MQ=255)
                                                                                                                                                          cccTGCTTGCTAAGCGCAAATTCAATGAACTGGAGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTc                                                                                        <  2:193373/250‑1 (MQ=255)
                                                                                                                                                          acCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTc                                                                                        <  1:900645/251‑1 (MQ=255)
                                                                                                                                                                      aacgcAAATTAGTAAAGCTGGCGCTGTTATGTTTTCATTCCTTCCCTCCTCACCTTACTTTGCTCCAGACAAAAAAATGCGACTCATCAGTCGCCTTAAAAATCTGTTGGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCAc                                                                            <  2:123858/249‑1 (MQ=255)
                                                                                                                                                                          cAAATTCAAAAGACGGGCTCTTATATTGTTTCATCCCTTCCCTCCTCTTCTTACTTTTCTACAGACAAAAAAAAGCGAGTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACg                                                                        <  2:959707/251‑1 (MQ=255)
                                                                                                                                                                                  aTAACCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTc                                                                <  1:325404/251‑1 (MQ=255)
                                                                                                                                                                                  aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTc                                                                <  1:460581/251‑1 (MQ=255)
                                                                                                                                                                                   tAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                                                               >  1:260703/1‑251 (MQ=255)
                                                                                                                                                                                              cTGATATGCTTTCATGCCTTCCCTCCTCATCTTACTTTTCTCCAGACAAAAAAAAGCGACCCCTCATTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCgg                                                     <  2:732408/251‑1 (MQ=255)
                                                                                                                                                                                                gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                                                                                                                          <  1:1004033/180‑1 (MQ=255)
                                                                                                                                                                                                     ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCca                                                                                                                                                                 <  2:789049/136‑1 (MQ=255)
                                                                                                                                                                                                                  cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACCCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATGcc                                 >  1:390508/1‑251 (MQ=255)
                                                                                                                                                                                                                   gCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCACCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGTCCTCCGCGGCAGAGTCTTCAACGTATTCCa                                >  1:730040/1‑251 (MQ=255)
                                                                                                                                                                                                                      cctcATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTTGCCTTCTTCCGTCCCCTGCACTTCAATGATGCGCCCGGCTTCGCTTGTCACTACGTTCATGTCGGTCTCTGCGGCAGCGCCTACACCGTCTTCCAgct                             >  2:388631/1‑249 (MQ=255)
                                                                                                                                                                                                                        tcATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatga                                                                                                  >  2:1004214/1‑180 (MQ=255)
                                                                                                                                                                                                                        tcATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCg                           >  1:370856/1‑251 (MQ=255)
                                                                                                                                                                                                                        tcATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCg                           >  1:670942/1‑251 (MQ=255)
                                                                                                                                                                                                                        tcATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGCCGCTACAATGGATTCGATTCCCCTCGGGCCCGACCCAACAAGATTCGTAGCTCTTCACGGTTCCACGCCTCGCCTTCTGCCGTCCCCTGCACTTCAATGTTGCGCCCGTCTTCGCTCATCCCTACGTTCCTGTCGGTTCCTTTGCCACATCCTTCAACGTCTTCCAGAttg                           >  2:988615/1‑249 (MQ=255)
                                                                                                                                                                                                                        tcATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTACGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAATATGAGTAGCTCTTAATAGGTGACCGGCCCGCCTTATCCCGTACCCGGCACTTCAATGATGGGCGCGTTTTCGGTCATACCTACGTTCATGTCGGTCGCTGCGGCAGGGTCTTCACCGTATTctacactc                           >  2:731813/1‑244 (MQ=255)
                                                                                                                                                                                                                        tcATCTTACTTTTCTACAGACAAAAAAA‑‑GCGACCCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCCTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCCTGTCGGCCTCTGCGGCAGCGTCCTCAACCTATTCCAGATCGc                          >  1:435657/1‑251 (MQ=255)
                                                                                                                                                                                                                             ttACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATggg                                                                                                                                         >  1:788899/1‑136 (MQ=255)
                                                                                                                                                                                                                               aCTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAACTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTGGATTACCGTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGTTCGCCTACTGACGGCCCCTGCACTTCAATGATGCGACCGTCTTCGGTCAGCACTACGGTAATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGACCGAAAAcag                    >  1:663493/1‑249 (MQ=255)
                                                                                                                                                                                                                                             caaaaaaaaGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGcc                                                                                            <  1:1015045/165‑1 (MQ=255)
                                                                                                                                                                                                                                               aataaaaGCGACTCATCAGGCGTCTTAAAACTGCGTTTTCCAGCGCCCCCTACTGCGTCGCTACAATGGATTCGATGCCCGTCGGGCCAGAGCCCACAAGATGAGTAGCTCTTCATGGGTGGACGTCTGACCTGCTGCCGTCCCCAGCACTTCAAGGAGGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACaa     <  2:198834/248‑1 (MQ=255)
                                                                                                                                                                                                                                                 aaaaAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCGGTACACAAtt   >  1:935824/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 aaaaAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTATTCGGTCATCACTACGTTCATGTCGTCCTCTGCGGCAGAGACTTCAACGTATTTCAGCTCGCAAACCGCTTCGCCGTTCGCAAtt   >  2:720597/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  aaaaGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGAATCCGATTTCTCTCGGGCCAGAGACAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCTTGCACTGCAATGACCCCCCCGTCTTCGTTTCTCACCACCTTCATGTGAGCCTCTCTGGCAAAGCCCTCACCGTATTCACACCCGAAAACCGCTTcccactctacactcc  >  2:253871/1‑237 (MQ=255)
                                                                                                                                                                                                                                                  aaaGGCGACTCAACAGGCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTc  <  2:248331/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       
AAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTC  >  NC_000913/3815565‑3816056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: