New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 3618253142 (0.970)4 (0.030) 4/448 12.9 2.7% coding (478/807 nt) nikE nickel ABC transporter ATPase
?NC_000913 = 3618266 147 (1.040)coding (491/807 nt) nikE nickel ABC transporter ATPase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

ACCGCTGGCGAAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCAGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGATCTCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTAAGCGGCGGCCAGCTCCAGCGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3617997‑3618253
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccagcgcgCGAGCCAGGCAGACGCGCTGGAGCTGGCCGCCGCTTAACTGCGGTGGGCGTTTGTCGAGAACGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGCGCGGATTCACGGCGCTGATGGAGTCCTGAAATACCATCTGAATATCGCGGCGGAACGCTTTACGCTGGG  <  NC_000913/3618266‑3618020
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
ACCGCTGGCGAAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCAGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGATCTCGATGACAGCGTTCTCGACAAACGCCAACCGCAGTTAAGCGGCGGCCAGCCCC                                                                                                                                                                                                                                                                >  1:775658/1‑251
ACCGCTGGCGAAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCAGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGATCTCGATGACAGCGTTCTCCACAAACGCCCACCCCAGTTAAGCTGCCGCCAGCCCC                                                                                                                                                                                                                                                                >  2:1437749/1‑251
ACCGCTGGCGAAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCAGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTAGCACTGAAAAAATCCGAACAACTGGCCCGCGCCAGCGAAATGCTGCAGGCGGTCGATCGCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTAAGCGGCGGCCAGCACC                                                                                                                                                                                                                                                                >  2:1347153/1‑251
  CGCTGGCGCAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCAGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCAACCTGCTATCACTGAAAAAATCCGACCAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGATCTCGATGACAGCGTTCTCGCCAAACGCCCACCGCAGTTAAGCGGCTGCAAGCCCCcg                                                                                                                                                                                                                                                              >  2:383828/1‑249
   GCTGGCGAAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCAGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGAACTCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTAAGCGGCGGCCAGCTCCAGC                                                                                                                                                                                                                                                             >  1:872800/1‑251
     TGGCGAAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCCGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCAAGCGAAATGCTGCAGGCGGTAGATCTCGATGACACCGTTCTCGACAAACGCCCACCGCAGTTAAGCGGCGGCCAGCTCCAGCcc                                                                                                                                                                                                                                                           >  1:578530/1‑249
     TGGCGAAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCAGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGATCTCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTCAGCGGCGGCACGCCCCAGgcg                                                                                                                                                                                                                                                           >  2:1313920/1‑248
     TGGCGAAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCAGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGATCTCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTACGCGGCGGCCAGCTCCAGCGC                                                                                                                                                                                                                                                           >  1:818906/1‑251
     TGGCGAAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCAGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGATCTCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTAAGCGGCGGCCAGCTCCAGCGC                                                                                                                                                                                                                                                           >  1:287966/1‑251
     TGGCGAAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCAGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGATCTCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTAAGCGGCGGCCAGCTCCAGCGC                                                                                                                                                                                                                                                           >  1:1163622/1‑251
     TGGCGAAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCAGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGATCTCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTAAGCGGCGGCCAGCTCCAGCGC                                                                                                                                                                                                                                                           >  1:1079565/1‑251
      ggctaacgcCAAGCGCGCCCAGCTTAAAGCGGTCGGCCGGTATATTCAGATGGTATTGCAGGACGCCATCAGCGCCGGGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGATCTCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTAAGCGGCGGCCAGCTCCAGCGCG                                                                                                                                                                                                                                                          <  2:1197748/242‑1
                                                                       CCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGATCTCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTAAGCGGCGGCCAGCTCCAGCG                                                                                                                                                                                                                                                            >  2:451133/1‑184
                                                                                                                                                                                               TCGATCTCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTAAGCGGCGGCCAGCTCCAGCGCGCGAGCCAGGCAGACGCGCTGGAGCTGGCCGCCGCTTAACTGCGGTGGGCGTTTGTCGAGAACGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGCGCGGATT                                                                 <  1:551207/251‑1
                                                                                                                                                                                                     TCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTAAGCGGCGGCCAGCTCCAGCGCGCGAGCCAGGCAGACGCGCTGGAGCTGGCCGCCGCTTAACTGCGGTGGGCGTCTGTCGAGAACGCGGTCATCGAGATCGACCGCCTTCAGGATTTCGCTGGCGTGCGGCAGGTCTGCGGATTTTTTCAGTGATAGGAGGTGGGCCACCGGTTCCCGCAGGAACGCGGTCAGGGTTTTGCGCGGGTTCcaggc                                                           >  2:551155/1‑246
                                                                                                                                                                                                                              CCCACCGCAGTTAAGCGGCGGCCAGCTCCAGCGCGCGAGCCAGGCAGACGCGCTGGAGCTGGCCGCCGCTTAACTGCGGTGGGCGTTTGTCGAGAACGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCCGCCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGCGCGGATTCACGGCGCCGATGGGGTCCTGAAATACCATC                                 >  1:623886/1‑251
                                                                                                                                                                                                                                   atcagtagtttcggttcgaccgCCAGCGCGCGAGCCAGGCAGACGCGCTGGAGCTGGCCGCCGCTTAACTGCGGTGGGCGTTTGTCGAGAACGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGCTTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGCTCTCTCGCACGGTTTTGCGCGGAGTCTCGGTGCTGTCTGGTGCCTCACATACCACCTGAAT                            >  2:395961/23‑251
                                                                                                                                                                                                                                           AGCGGCGGCCACCTCCGGCGCGCGAGCCAGGCAGACGCGCTGGGCCTGGCCTCCGCTTAACTGCGGTGGGCGTTTGTCGAGAACGCTGTCATCGGGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGCGCGGATTCACGGCGCTGATGGAGTCCTGAAATACCATCTGAATATCGCGGC                    <  2:623823/251‑1
                                                                                                                                                                                                                                                         CCAGCGCGCGAGCCAGGCAGACGCGCTGGAGCTGGCCGCCGCTTAACTGCGGTGGGCGTTTGTCGAGAACGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGCGCGGATTCACGGCGCTGATGGAGTCCTGAAATACCATCTGAATATCGCGGCGGAACGCTTTACGC      >  1:188550/1‑251
                                                                                                                                                                                                                                                          CAGCGCGCGAGCCAGGCAGACGCGCTGGAGCTGGCCGCCGCTTAACTGCGGTGGGCGTTTGTCGAGAACGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGCGCGGATTCACGGCGCTGATGGAGTCCTGAAATACCATCTGAA                             >  1:785508/1‑227
                                                                                                                                                                                                                                                           AGCGCGCGAGCCAGGCAGACGCGCTGGAGCTGGCCGCCGCTTAACTGCGGTGGGCGTTTGTCGAGAACGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGCGCGGATTCACGGCGCTGATGGAGTCCTGAAATACCATCTGAATATCGCGGCGGAACGCTTTACGCTG    <  1:378120/251‑1
                                                                                                                                                                                                                                                             CGCGCGAGCCAGGCAGACGCGCTGGAGCTGGCCGCCGCTTAACTGCGGTGGGCGTTTGTCGAGAACGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGCGCGGATTCACGGCGCTGATGGAGTCCTGAAATACCATCTGAATATCGCGGCGGACCGCTTTACGCTAGG  >  2:1335337/1‑251
                                                                                                                                                                                                                                                             CGCGCGAGCCAGGCAGACGCGCTGGAGCTGGCCGCCGCTTAACTGCGGTGGGCGTTTGTCGAGAACGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGCGCGGATTCACGGCGCTGATGGAGTCCTGAAATACAATCTGAATATCGCGGCGGAACGCTTTACGCTGGG  >  1:680975/1‑251
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
ACCGCTGGCGAAACTCAATCGCGCCCAGCGTAAAGCGTTCCGCCGCGATATTCAGATGGTATTTCAGGACTCCATCAGCGCCGTGAATCCGCGCAAAACCGTGCGCGAGATCCTGCGTGAACCGATGCGCCACCTGCTATCACTGAAAAAATCCGAACAACTGGCGCGCGCCAGCGAAATGCTGAAGGCGGTCGATCTCGATGACAGCGTTCTCGACAAACGCCCACCGCAGTTAAGCGGCGGCCAGCTCCAGCGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3617997‑3618253
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccagcgcgCGAGCCAGGCAGACGCGCTGGAGCTGGCCGCCGCTTAACTGCGGTGGGCGTTTGTCGAGAACGCTGTCATCGAGATCGACCGCCTTCAGCATTTCGCTGGCGCGCGCCAGTTGTTCGGATTTTTTCAGTGATAGCAGGTGGCGCATCGGTTCACGCAGGATCTCGCGCACGGTTTTGCGCGGATTCACGGCGCTGATGGAGTCCTGAAATACCATCTGAATATCGCGGCGGAACGCTTTACGCTGGG  <  NC_000913/3618266‑3618020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.