New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2594668	87 (0.850)	4 (0.040)	4/464	9.1	4.3%	coding (1192/2016 nt)	tmcA	elongator methionine tRNA (ac4C34) acetyltransferase
	?	NC_000913	= 2594881	92 (0.910)					coding (979/2016 nt)	tmcA	elongator methionine tRNA (ac4C34) acetyltransferase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

AAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTATCCGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCGCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2594418‑2594668 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgGAAAAAATGGTCAGCGAGGCACTGGTTTTTGACGATGAAAACTTCACCCATACACCACAAGGCAATATTGTCATTTCCGCATTTGAACAGACGTTATGGCAAAGCGATCCAGAAACGCCGTTAAAGGTTTATCAGCTCTTGTCTGGTGCGCACTATCGGACTTCGCCGCTGGATTTACGCCGGATGATGGATGCACCAGGGCAACATTTTTTACAGGCGGCTGGCGAAAACGA  <  NC_000913/2594881‑2594649                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      AAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTATCCGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCGCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTG                                                                                                                                                                                                                                           >  1:810659/1‑251         AGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTATCCGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCGCCTGACTGAGTTGTTGAGATAATCCACCCCCATCCACCAGCCACAGCGCCCCGGAAATCCCGTTTTCCCCAGCCGCCTGGAAAAAAT                                                                                                                                                                                                                                   >  2:743693/1‑251                                                                             GTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCGCCTGACTGAGTTGTTGAGATAATCCACCCCCATCCACCAGCCACAGCGCCCCGGCAATCCCGTTTTCGCCAGCCGCCTGGAAAAAAGGGTCAGCGAGGCACTGGTTTTTGCCGATGAAAACTTCACCCATACACCACAAGGCAATATTGTCATTT                                                                                                                                                               >  2:801447/1‑251                                                                                                                                                                                        TGTTGAGCTAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGGAAAAAATGGTCAGCGAGGCACTGGTTTTTGACGATGAAAACTTCACCCATACACCACAAGGCAATATTGTCATTTCCGCATTTGAACAGACGTTATGGCAAAGCGATCCAGAAACGCCGTTAAAGGTTTATCAGCTCTTGTCTGGTGCGCACTATCGGACTTCGCCGCTGGATTTACGCCGG                                                    <  1:801304/251‑1                                                                                                                                                                                                                                          gcgaaggggagcacCCTGGAAAAAATGGTCAGGGAGGCACTGATTTTTGACGATGAAAACTGCCCCCGTACACAACAAAGCAATATTGACATTTCCGCATTTGAACAGACGTTATGGCAAAGCGATCCATAAACGCCGTTAGAGGTTTATCAGCTCTGGTCTGGTGCGCACTGTCGGACTTCGCCGCTGGATTGACGCCGGATGATGGATGCACCAGGGCAACATTTTTTACAGGCGGATGGCGAAAACGA  <  1:592054/237‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      AAAGCACCAGCAATAAGTTGCCGCCCTGTGCCTTCCCGCTGACGAGCCGGATGAACTGCTATCCGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCGCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2594418‑2594668 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgGAAAAAATGGTCAGCGAGGCACTGGTTTTTGACGATGAAAACTTCACCCATACACCACAAGGCAATATTGTCATTTCCGCATTTGAACAGACGTTATGGCAAAGCGATCCAGAAACGCCGTTAAAGGTTTATCAGCTCTTGTCTGGTGCGCACTATCGGACTTCGCCGCTGGATTTACGCCGGATGATGGATGCACCAGGGCAACATTTTTTACAGGCGGCTGGCGAAAACGA  <  NC_000913/2594881‑2594649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.