Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913894,9520TA21.2% 67.4 / 14.4 33intergenic (+56/‑39)potF/potGputrescine ABC transporter periplasmic binding protein/putrescine ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (12/14);  new base A (3/4);  total (15/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTCGTGCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGT  >  NC_000913/894805‑895094
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ttCGTGCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCAtt                                                                                                                                                <  7:133679/148‑1 (MQ=255)
    tGCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGt                                                                                                                                           >  1:41591/1‑149 (MQ=255)
      cGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgc                                                                                                                                         <  2:41591/149‑1 (MQ=255)
       gAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgc                                                                                                                                         >  4:38851/1‑148 (MQ=255)
         aGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgcgaa                                                                                                                                      <  3:38851/149‑3 (MQ=255)
             gTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgcgaattgc                                                                                                                                  <  2:185775/149‑7 (MQ=255)
              ttCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCtt                                                                                                                                     >  5:100182/1‑145 (MQ=255)
              ttCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCtt                                                                                                                                     <  6:100182/145‑1 (MQ=255)
              ttCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCACAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGcac                                                                                                                                 >  3:89283/1‑149 (MQ=255)
               tCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGcaca                                                                                                                                >  1:69544/1‑149 (MQ=255)
                           tGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCg                                                                                                                    <  6:156033/149‑1 (MQ=255)
                               ggATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGgaga                                                                                                                <  7:61016/149‑1 (MQ=255)
                                    cGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGagcagc                                                                                                           <  4:118200/149‑1 (MQ=255)
                                        aaTCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgc                                                                                                                                         >  3:32897/1‑115 (MQ=255)
                                        aaTCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATAATGGTgc                                                                                                                                         <  4:32897/115‑1 (MQ=255)
                                            gACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAAt                                                                                                    <  8:146875/148‑1 (MQ=255)
                                                               cgTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGcac                                                                                                                                 >  8:22763/1‑100 (MQ=255)
                                                               cgTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGcac                                                                                                                                 <  7:22763/100‑1 (MQ=255)
                                                                gTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAg                                                                               >  6:112223/1‑149 (MQ=255)
                                                                             aaGAGCAGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGt                                                                   >  2:169796/1‑148 (MQ=255)
                                                                                agCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAgg                                                               <  5:112223/149‑1 (MQ=255)
                                                                                     aaaaTAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTg                                                          >  3:129608/1‑149 (MQ=255)
                                                                                                             cGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACAATCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAAc                                  <  7:45270/149‑1 (MQ=255)
                                                                                                                          cacaCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGgcgc                                                            >  4:89645/1‑110 (MQ=255)
                                                                                                                          cacaCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGgcgc                                                            <  3:89645/110‑1 (MQ=255)
                                                                                                                          cacaCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATcc                      >  1:74954/1‑148 (MQ=255)
                                                                                                                             acCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACAt                                                                                                                             >  7:125927/1‑42 (MQ=255)
                                                                                                                             acCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACAt                                                                                                                             <  8:125927/42‑1 (MQ=255)
                                                                                                                             acCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTAc                   >  6:71480/1‑148 (MQ=255)
                                                                                                                               ccgccgGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGaa                                                                                                     <  2:111197/64‑1 (MQ=255)
                                                                                                                               ccgccgGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGaa                                                                                                     >  1:111197/1‑64 (MQ=255)
                                                                                                                                      cAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAAc         <  1:169796/149‑1 (MQ=255)
                                                                                                                                       aaTTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACa        <  4:89283/149‑1 (MQ=255)
                                                                                                                                             cACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGt  <  2:74954/149‑1 (MQ=255)
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TTCGTGCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGACGCCGCTATTAGAAATCCGCAACCTGACCAAATCCTACGATGGTCAACATGCGGT  >  NC_000913/894805‑895094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑