Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 169,078 C→A S532* (TCG→TAG)  fhuA → ferrichrome outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913169,0780CA100.0% 133.3 / NA 39S532* (TCG→TAG) fhuAferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/20);  total (19/20)

ATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTCCGATTGTAGTTACTGGTGCCGTGTATAATCTCACTAAAACC  >  NC_000913/168952‑169220
                                                                                                                              |                                                                                                                                              
aTCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGtt                                                                                                                          >  6:40746/1‑149 (MQ=255)
aTCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGtt                                                                                                                          >  2:48804/1‑149 (MQ=255)
   aaGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTgg                                                                                                                        <  1:111258/148‑1 (MQ=255)
   aaGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGATTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTgg                                                                                                                        >  3:113151/1‑148 (MQ=255)
            ttAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATgg                                                                                                               >  5:57994/1‑148 (MQ=255)
              aaCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGt                                                                                                              >  1:93254/1‑147 (MQ=255)
              aaCCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGt                                                                                                              <  2:93254/147‑1 (MQ=255)
                     ttGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTc                                                                                                     <  8:7552/149‑1 (MQ=255)
                                  cGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGt                                                                                                              <  6:60187/127‑1 (MQ=255)
                                  cGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGt                                                                                                              >  5:60187/1‑127 (MQ=255)
                                  cGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTc                                                                                                     <  4:39906/136‑1 (MQ=255)
                                  cGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTc                                                                                                     >  3:39906/1‑136 (MQ=255)
                                   gATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAgg                                                                                       >  3:23746/1‑149 (MQ=255)
                                       aaCGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATg                                                                                                                >  3:112731/1‑120 (MQ=255)
                                       aaCGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATg                                                                                                                <  4:112731/120‑1 (MQ=255)
                                            gatgaCAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTa                                                                              >  8:77667/1‑149 (MQ=255)
                                             atgaCAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTa                                                                              <  5:40746/148‑1 (MQ=255)
                                               gaCAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATg                                                                            >  6:127973/1‑148 (MQ=255)
                                                  aaaCAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGt                                                                        >  4:140605/1‑149 (MQ=255)
                                                           aCCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGa                                                                <  5:127973/148‑1 (MQ=255)
                                                            ccTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGaaa                                                              <  6:57994/149‑1 (MQ=255)
                                                                               cTACCTGTTTGATTATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTATAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTc                                           <  4:113151/149‑1 (MQ=255)
                                                                                  ccTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAgg                                                                                       <  7:136623/102‑1 (MQ=255)
                                                                                  ccTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAatat                                                           >  1:146975/1‑130 (MQ=255)
                                                                                  ccTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAatat                                                           <  2:146975/130‑1 (MQ=255)
                                                                                  ccTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTCCg                                         <  3:140605/148‑1 (MQ=255)
                                                                                  ccTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTAGCACCGTCTAAAgg                                                                                       >  8:136623/1‑102 (MQ=255)
                                                                                          aTAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTCCGATTGTAGtt                                <  4:23746/149‑1 (MQ=255)
                                                                                           tAATGGTGTAACACCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTAGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGAACAGGAAGATTGTCCGATTGTAGTTa                               >  1:106640/1‑149 (MQ=255)
                                                                                                     acacCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTCCGATTGTAGTTACTGGTGCCgt                     <  1:48804/149‑1 (MQ=255)
                                                                                                       acCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGaa                                                                          >  4:47424/1‑94 (MQ=255)
                                                                                                       acCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTCCGAtt                                      <  5:75485/130‑1 (MQ=255)
                                                                                                       acCTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTCCGAtt                                      >  6:75485/1‑130 (MQ=255)
                                                                                                         cTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGt                                                                  >  2:82568/1‑100 (MQ=255)
                                                                                                         cTTACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGt                                                                  <  1:82568/100‑1 (MQ=255)
                                                                                                          ttACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGt                                                                               <  3:113748/86‑1 (MQ=255)
                                                                                                          ttACTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGt                                                                               >  4:113748/1‑86 (MQ=255)
                                                                                                            aCTTCAGCTATAGCGAATAGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTCCGATTGTAGTTACTGGTGCCGTGTATaa               <  7:77667/148‑1 (MQ=255)
                                                                                                                        gCGAATAGTTTGAACATTCTTCTAAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGTTAAGCAGTATGAAGGAGGCGTGAAATATGTACCGTAAGATTGTCCGATTGTAGTTACTGGTGCCGTGTATAATCTCACTAAAAcc  <  2:106640/149‑1 (MQ=255)
                                                                                                                              |                                                                                                                                              
ATCAAGAATCTCTTAACCGCGTTGCCGGGACGACCGATAAACGTGATGACAAACAGTTTACCTGGCGTGGTGGTGTTAACTACCTGTTTGATAATGGTGTAACACCTTACTTCAGCTATAGCGAATCGTTTGAACCTTCTTCGCAAGTTGGGAAGGATGGTAATATTTTCGCACCGTCTAAAGGTAAGCAGTATGAAGTCGGCGTGAAATATGTACCGGAAGATCGTCCGATTGTAGTTACTGGTGCCGTGTATAATCTCACTAAAACC  >  NC_000913/168952‑169220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑