Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,801,032 C→T G323E (GGA→GAA)  waaR ← lipopolysaccharide 1,2‑glucosyltransferase; UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,801,0320CT97.1% 474.9 / 0.2 141G323E (GGA→GAA) waaRlipopolysaccharide 1,2‑glucosyltransferase; UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base T (58/78);  total (59/82)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

TCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTCCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAATTGCC  >  NC_000913/3800886‑3801175
                                                                                                                                                  |                                                                                                                                               
  aTTTAATACATTGATGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTga                                                                                                                                             <  6:130355/149‑1 (MQ=255)
  aTTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATACACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTga                                                                                                                                             <  6:218865/149‑1 (MQ=255)
  aTTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTga                                                                                                                                             <  5:49148/149‑1 (MQ=255)
   tttAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCt                                                                                                                                               >  8:45778/1‑146 (MQ=255)
   tttAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTgag                                                                                                                                            <  1:270391/149‑1 (MQ=255)
   tttAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTgag                                                                                                                                            <  1:61619/149‑1 (MQ=255)
       aTACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGCCTCCTGCTATAATTTCTGAGatat                                                                                                                                        >  8:248984/1‑149 (MQ=255)
       aTACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGatat                                                                                                                                        <  6:174559/149‑1 (MQ=255)
        tACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGAtata                                                                                                                                       <  2:122200/149‑1 (MQ=255)
          cATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATaa                                                                                                                                      <  4:151755/148‑1 (MQ=255)
          cATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAat                                                                                                                                     <  6:265913/149‑1 (MQ=255)
           aTTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAatg                                                                                                                                    <  7:212557/149‑1 (MQ=255)
           aTTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAatg                                                                                                                                    <  2:287025/149‑1 (MQ=255)
           aTTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAat                                                                                                                                     >  4:174522/1‑148 (MQ=255)
                 gTACTTTGAATTGTTTTCATCTGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCATTTAAATGTTATTTACGGTAATATTTTAGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTg                                                                                                                              <  4:299160/149‑1 (MQ=255)
                 gTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTg                                                                                                                              <  7:190119/149‑1 (MQ=255)
                    cTTTGCATTGTTTTCATCGGTAAAAACACACAAGTCTGTGATCTTGCTCTTCTGAATCATAATATACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCa                                                                                                                            <  6:215002/148‑1 (MQ=255)
                    cTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCAc                                                                                                                           >  5:199918/1‑149 (MQ=255)
                     tttGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCAc                                                                                                                           >  1:325223/1‑148 (MQ=255)
                      ttGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACt                                                                                                                          >  5:155364/1‑148 (MQ=255)
                       tGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTa                                                                                                                         >  6:236196/1‑148 (MQ=255)
                          aTTGTTTTCATGGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACTGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGtt                                                                                                                               >  4:212417/1‑139 (MQ=255)
                          aTTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTaa                                                                                                                        >  3:319188/1‑146 (MQ=255)
                           ttGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGa                                                                                                                    <  8:296864/149‑1 (MQ=255)
                              ttttCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATAATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTCCTGAGATATAATGATGTTGCACTAAAAGATGt                                                                                                                 <  7:130608/149‑1 (MQ=255)
                                ttCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGtt                                                                                                                <  6:8138/148‑1 (MQ=255)
                                 tCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGttt                                                                                                               <  8:189093/148‑1 (MQ=255)
                                 tCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGttt                                                                                                               <  6:51863/148‑1 (MQ=255)
                                 tCATCGGTAAAAACAAACAAGTCTTTGATCTTGCGCTTCTGAATCATTATAAACCAGTTAAATGTTATTTACGTTAATATTTTCGTGAAAGATAACAGACTCCTGCTATTATTTCTGAGATATAATGATGTTGCACTAAAAGATGttt                                                                                                               <  2:27046/148‑1 (MQ=255)
                                 tCATCGGGAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGttt                                                                                                               <  6:12086/148‑1 (MQ=255)
                                   aTCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTtata                                                                                                            <  8:109237/149‑1 (MQ=255)
                                   aTCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGATAAATGTTATTTACGTTAATATTTTCGCCAAAGATAACAGACTCCTGCTATAATTTCAGAGATATAATGATGTTGCACTAAAATATGTTtata                                                                                                            >  1:45588/1‑149 (MQ=255)
                                       gTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCtt                                                                                                        >  1:156065/1‑149 (MQ=255)
                                        tAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATACCAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCttt                                                                                                       >  4:299804/1‑149 (MQ=255)
                                        tAAAAACAACCAAGTCTTTGATCTTGCGCTTCAGAATCATAAAAAACCAGTTAAATGTTATTAACCGTTATATTTTCGGCAAAGATAACAGACTCCGGCTATAAATTCTGAGATATAATGAAGTTGCACTAATAGATGTTTATATCttt                                                                                                       >  8:392727/1‑149 (MQ=255)
                                           aaaCAATCAAGTCTTTGATATTTCTATTCTTAATCATAATAAAACATTTAAATGTTATTTACGGTAATATTTTCGTCAAATATAACTGAATCCTGCTATAATTTCTGAGATATATTGATGTTGCACTAAAAGATGt                                                                                                                 <  5:401842/136‑1 (MQ=255)
                                           aaaCAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCtttttt                                                                                                    <  6:155364/149‑1 (MQ=255)
                                                     gTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCaa                                                                                           >  2:175706/1‑148 (MQ=255)
                                                      tCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCaata                                                                                         <  3:340509/149‑1 (MQ=255)
                                                      tCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCaata                                                                                         <  8:223145/149‑1 (MQ=255)
                                                        tttGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTtat                                                                                                             >  7:374707/1‑127 (MQ=255)
                                                          tGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAAtt                                                                                      <  6:169250/148‑1 (MQ=255)
                                                          tGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTAAGTTAATATTTTCGTCAAAGATAACAGACTCCTTCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAAtt                                                                                      <  1:169066/148‑1 (MQ=255)
                                                              cTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATATCAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTc                                                                                             >  6:228413/1‑137 (MQ=255)
                                                              cTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTc                                                                                             <  5:228413/137‑1 (MQ=255)
                                                                tGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATTTAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCg                                                                               >  8:237382/1‑149 (MQ=255)
                                                                 gCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGAt                                                                                   <  6:361804/144‑1 (MQ=255)
                                                                 gCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTc                                                                                <  6:192654/147‑1 (MQ=255)
                                                                 gCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCg                                                                               >  8:114466/1‑148 (MQ=255)
                                                                  ctctTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCTGCATAGATATCAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCga                                                                              <  1:64628/148‑2 (MQ=255)
                                                                    cttctGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCAtc                                                                           >  7:1616/1‑149 (MQ=255)
                                                                     ttctGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCAtct                                                                          >  2:171310/1‑149 (MQ=255)
                                                                     ttctGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCAtct                                                                          >  7:24214/1‑149 (MQ=255)
                                                                       ctGAATCATAATAAACCAGTTAAATGTTATTTACGTTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCAtctc                                                                         <  4:176742/148‑1 (MQ=255)
                                                                           aTCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGga                                                                    <  8:61729/149‑1 (MQ=255)
                                                                             cATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGga                                                                    >  5:19705/1‑147 (MQ=255)
                                                                              ataataATCTAGTTTAATGTTATTTACTGTAATATTTTGTGCAAAGATTAGAGACTCCTGCTTTAATTGCTGAGATTTAATGATGTTTCAGTAAAAGTTGTTTATATCTTTTTTTAAATTCTATAATTTATTTCGCATCTCGTggtgt                                                                  <  8:261802/148‑4 (MQ=255)
                                                                              ataataAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGgaga                                                                  <  5:236196/148‑1 (MQ=255)
                                                                                aataaACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGt                                                                >  8:394073/1‑148 (MQ=255)
                                                                                  taaACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTCCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCAt                                                             <  2:24825/149‑1 (MQ=255)
                                                                                       cAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTc                                                        <  1:275807/149‑1 (MQ=255)
                                                                                       cAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTc                                                        >  8:225134/1‑149 (MQ=255)
                                                                                         gTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGt                                                                       >  5:127132/1‑132 (MQ=255)
                                                                                         gTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGt                                                                       <  6:127132/132‑1 (MQ=255)
                                                                                              aTGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAgggg                                                  >  7:407847/1‑148 (MQ=255)
                                                                                                gTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGaa                                               >  3:121740/1‑149 (MQ=255)
                                                                                                gTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATATAGTGGAGAGTCATCTTTACAtggttaa                                               >  7:377013/1‑145 (MQ=255)
                                                                                                 ttATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGaa                                               <  7:402849/148‑1 (MQ=255)
                                                                                                   aTTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAAtt                                             <  3:305926/148‑1 (MQ=255)
                                                                                                    tttACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAAtttt                                           >  7:122629/1‑149 (MQ=255)
                                                                                                     ttACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTc                                          >  7:30624/1‑149 (MQ=255)
                                                                                                      tACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTc                                          <  6:75573/148‑1 (MQ=255)
                                                                                                        cGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTa                                        <  2:285973/148‑1 (MQ=255)
                                                                                                        cGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTa                                        <  7:45778/148‑1 (MQ=255)
                                                                                                        cGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTa                                        >  1:357330/1‑148 (MQ=255)
                                                                                                         ggTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTaaa                                      <  3:231354/149‑1 (MQ=255)
                                                                                                         ggTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTaaa                                      <  2:235046/149‑1 (MQ=255)
                                                                                                           tAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCAt                                                                            <  2:4717/109‑1 (MQ=255)
                                                                                                           tAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGc                                    >  4:242233/1‑149 (MQ=255)
                                                                                                           tAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGc                                    >  1:184403/1‑149 (MQ=255)
                                                                                                             atatTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTa                                  >  4:228423/1‑149 (MQ=255)
                                                                                                              tatTTTCGGCAAAGATAACATACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTAGTGGAGAGTCATCTTTCCAGGGGGAATTTTATAAAGCTAt                                 >  7:151674/1‑149 (MQ=255)
                                                                                                              tatTTTCGGCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTAt                                 <  6:65791/149‑1 (MQ=255)
                                                                                                                     ggCAAAGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTtata                           <  7:225134/148‑1 (MQ=255)
                                                                                                                         aaGATAACAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTAtt                      <  2:156065/149‑1 (MQ=255)
                                                                                                                             tAACAGACTCCTGCTATAATTTCTGATATATAATGATGTTGCACTAAAATATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCTCATCTCTTGTAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTAc                  <  3:236248/149‑1 (MQ=255)
                                                                                                                                cAGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTGTATATATTTTTTTAAATTAAATAATTGATTTCGCATCAAGAGTATATTAATCTTTCAAGGGTGAATTTTATAAAGCTATTTTATATTATTTTa                   >  5:156028/1‑145 (MQ=255)
                                                                                                                                 aGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGAt              <  4:100092/149‑1 (MQ=255)
                                                                                                                                 aGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGAt              >  5:154152/1‑149 (MQ=255)
                                                                                                                                 aGACTCCTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGAt              <  5:286885/149‑1 (MQ=255)
                                                                                                                                  gACTCCTGCTATAATTTCTGAGATATAATGATGTTTCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATg             <  2:45588/149‑1 (MQ=255)
                                                                                                                                       cTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTtata                           <  3:102590/130‑1 (MQ=255)
                                                                                                                                       cTGCTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTtata                           >  4:102590/1‑130 (MQ=255)
                                                                                                                                          cTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGga                                                                    <  2:160428/86‑1 (MQ=255)
                                                                                                                                          cTATAATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAAt      <  3:146494/148‑1 (MQ=255)
                                                                                                                                           tataATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGGTATTTTATAGTATTTTACCGATGGATAAATTg    >  5:124767/1‑149 (MQ=255)
                                                                                                                                            ataATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAATTGc   >  4:51273/1‑149 (MQ=255)
                                                                                                                                            ataATTCCTCCCAAAAAAGGAGGTTGCACAAAACCATGTTAAATCCTTTTTTAAAATTAAAAAATTCATTTCGCACCCCGGGTCTCGGATCCTTCCCCGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAATTGcc  <  8:263243/149‑1 (MQ=255)
                                                                                                                                             taATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAATTGcc  <  4:319188/149‑1 (MQ=255)
                                                                                                                                             taATTTCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAATTGc   <  7:263280/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TCATTTAATACATTGAGGTACTTTGAATTGTTTTCATCGGTAAAAACAACCAAGTCTTTGATCTTGCTCTTCTGAATCATAATAAACCAGTTAAATGTTATTTACGGTAATATTTTCGGCAAAGATAACAGACTCCTGCTATAATTCCTGAGATATAATGATGTTGCACTAAAAGATGTTTATATCTTTTTTTAAATTCAATAATTGATTTCGCATCTCGTGGAGAGTCATCTTTCCAGGGGGAATTTTCTAAAGCTATTTTATAGTATTTTACCGATGGATAAATTGCC  >  NC_000913/3800886‑3801175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: