Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 414,144 T→G 6.2% N537T (AAC→ACC)  sbcC ← exonuclease, dsDNA, ATP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913414,1440TG 6.2% 200.8 / 4.2 65N537T (AAC→ACC) sbcCexonuclease, dsDNA, ATP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (29/31);  new base G (2/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

TGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTT  >  NC_000913/414001‑414273
                                                                                                                                               |                                                                                                                                 
tGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTc                                                                                                                               <  4:356637/148‑1 (MQ=255)
 gACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTc                                                                                                                               >  5:60301/1‑147 (MQ=255)
 gACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATAACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTc                                                                                                                               >  1:133650/1‑147 (MQ=255)
 gACGGCTTACCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTc                                                                                                                               >  4:204183/1‑147 (MQ=255)
   cGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTACCGTCGCACATTCTTCACCTAGCTTTTTAACTTCGt                                                                                                                                   >  6:325031/1‑141 (MQ=255)
      cTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAgcgc                                                                                                                         >  5:237557/1‑148 (MQ=255)
          ccATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAg                                                                                                                      >  7:140153/1‑147 (MQ=255)
              tgttgAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTc                                                                                                                               >  5:143793/1‑134 (MQ=255)
              tgttgAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTc                                                                                                                               <  6:143793/134‑1 (MQ=255)
              tgttgAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGAGTCCAGTTGCCCCCGTAGCGTCGCACCTTCTTGCCCGAGCTGTTTAACTTCGATTTCCAGCGCCAGTAATc                                                                                                                 >  4:310452/1‑148 (MQ=255)
                 tgAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGAGCTTCGTTTTCATCACGATGAAGCTGCTTTGTTATGGCGTACAGTTGCCAACGTAGCGTCGCACATTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCAAGTAATc                                                                                                                 >  1:311977/1‑145 (MQ=255)
                  gAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCgag                                                                                                              <  2:133650/147‑1 (MQ=255)
                  gAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCgag                                                                                                              <  3:204183/147‑1 (MQ=255)
                  gAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCgag                                                                                                              <  8:140153/147‑1 (MQ=255)
                   aGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGAc                                                                                                            <  6:60301/148‑1 (MQ=255)
                        gTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGAt                                                                                                        >  3:217144/1‑147 (MQ=255)
                         tgcttgctCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTa                                                                                                      <  6:237557/148‑1 (MQ=255)
                               ctCATCTTGTAGGAGTCTTTGCGCTTCGTTTTCATAACGCTGAAGCTGCTTTGTTTTGGCGTCCAGTTGACCACGTAGCTTCGCACCTTCATCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGcc                                                                                                 <  2:311977/147‑1 (MQ=255)
                                  aTCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAg                                                                                               <  4:356061/146‑1 (MQ=255)
                                   tCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTa                                                                                                      >  2:125664/1‑138 (MQ=255)
                                   tCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTa                                                                                                      <  1:125664/138‑1 (MQ=255)
                                       gTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTcc                                                                                          <  3:310452/146‑1 (MQ=255)
                                        tCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAgc                                                                                       >  1:252460/1‑148 (MQ=255)
                                           gAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTaa                                                                                                     <  2:90520/131‑1 (MQ=255)
                                           gAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTaa                                                                                                     >  1:90520/1‑131 (MQ=255)
                                               cTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTcc                                                                                          <  3:310091/138‑1 (MQ=255)
                                               cTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTcc                                                                                          >  4:310091/1‑138 (MQ=255)
                                               cTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGAt                                                                                <  3:213452/148‑1 (MQ=255)
                                                  tGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACg                                                                             >  6:138333/1‑148 (MQ=255)
                                                    cgcTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGcc                                                                           >  8:188503/1‑148 (MQ=255)
                                                        tCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGcc                                                                                    <  2:269555/135‑1 (MQ=255)
                                                        tCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGcc                                                                                    >  1:269555/1‑135 (MQ=255)
                                                            tttCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGAccgc                                                                   >  3:55862/1‑148 (MQ=255)
                                                            tttCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGAccgc                                                                   >  1:216754/1‑148 (MQ=255)
                                                              tcatcaCGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGAccgcc                                                                  <  6:125930/147‑1 (MQ=255)
                                                               catcaCGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGAccgccg                                                                 <  5:277582/147‑1 (MQ=255)
                                                               catcaCGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGAccgccg                                                                 <  4:217144/147‑1 (MQ=255)
                                                                           gctgctTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGa                                                    <  7:190683/148‑1 (MQ=255)
                                                                                  tGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACcaca                                              <  2:252460/147‑1 (MQ=255)
                                                                                      aTGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGt                                          <  7:188503/147‑1 (MQ=255)
                                                                                         gCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGc                                      <  2:216754/148‑1 (MQ=255)
                                                                                          cGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCa                                     <  5:138333/148‑1 (MQ=255)
                                                                                           gTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAgcgc                                                                                     >  4:286/1‑99 (MQ=255)
                                                                                           gTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAgcgc                                                                                     <  3:286/99‑1 (MQ=255)
                                                                                             ccAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGa                                                    <  2:200960/130‑1 (MQ=255)
                                                                                             ccAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGa                                                    >  1:200960/1‑130 (MQ=255)
                                                                                                gTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGGTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTTGTGGAACCACaaa                                            >  3:12487/1‑135 (MQ=255)
                                                                                                gTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGGTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACaaa                                            <  4:17430/135‑1 (MQ=255)
                                                                                                gTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGGTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACaaa                                            >  3:17430/1‑135 (MQ=255)
                                                                                                gTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGGTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACaaa                                            <  4:12487/135‑1 (MQ=255)
                                                                                                  tGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGAc                             >  1:382047/1‑148 (MQ=255)
                                                                                                        cGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACATCGTTTTCCAGCGCCAGTAATCGAGACTGAATAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGATGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCt                       >  1:263374/1‑148 (MQ=255)
                                                                                                           aGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGt                                          <  6:214554/126‑1 (MQ=255)
                                                                                                           aGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGt                                          >  5:214554/1‑126 (MQ=255)
                                                                                                                    ccTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCAc           <  4:55862/148‑1 (MQ=255)
                                                                                                                     cttcttCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGa                                                    >  7:10039/1‑106 (MQ=255)
                                                                                                                     cttcttCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGa                                                    <  8:10039/106‑1 (MQ=255)
                                                                                                                      ttcttcACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGt         >  4:134698/1‑148 (MQ=255)
                                                                                                                       tcttcACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGtt        >  4:118372/1‑148 (MQ=255)
                                                                                                                        cttcACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGc                                 <  3:94439/122‑1 (MQ=255)
                                                                                                                        cttcACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGc                                 >  4:94439/1‑122 (MQ=255)
                                                                                                                             ccGAGCTTTTTAACTTCGTTTTCCAGCACCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCtt  >  7:136916/1‑148 (MQ=255)
                                                                                                                                       tAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTg                               >  4:9612/1‑109 (MQ=255)
                                                                                                                                       tAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTg                               <  3:9612/109‑1 (MQ=255)
                                                                                                                                             cGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTa                    >  6:168751/1‑114 (MQ=255)
                                                                                                                                             cGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTa                    <  5:168751/114‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                 
TGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAACTTCGTTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACCACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTT  >  NC_000913/414001‑414273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: