Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 191,997 G→A 5.6% G48D (GGT→GAT)  pyrH → uridylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913191,9970GA 5.6% 238.4 / 2.9 71G48D (GGT→GAT) pyrHuridylate kinase
Reads supporting (aligned to +/- strand):  ref base G (32/35);  new base A (2/2);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

AAACATGGCTACCAATGCAAAACCCGTCTATAAACGCATTCTGCTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCA  >  NC_000913/191851‑192141
                                                                                                                                                  |                                                                                                                                                
aaaCATGGCTACCAATGCAAAACCCGTCTATAAACGCATTCAGCTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTggg                                                                                                                                                  >  7:273932/1‑147 (MQ=255)
          aCCAATGCAAAACCCGTCTATAAACGCATTCTGCTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTtg                                                                                                                                       <  4:217428/148‑1 (MQ=255)
               tGCAAACCCCGTCTATAAACGCATTCTGCTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTtggtgtg                                                                                                                                  >  1:108851/1‑148 (MQ=255)
                  aaaaCCCGTCTATAAACGCATTCTGCTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTtggtgtggtg                                                                                                                               <  3:61093/148‑1 (MQ=255)
                  aaaaCCCGTCTATAAACGCATTCAGCTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTtggtgtggtg                                                                                                                               <  8:273932/148‑1 (MQ=255)
                     aCCCGTCTATAAACGCATTCTGCTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCa                                                                                                                                            <  7:267787/132‑1 (MQ=255)
                     aCCCGTCTATAAACGCATTCTGCTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCa                                                                                                                                            >  8:267787/1‑132 (MQ=255)
                        cGTCTATAAACGCATTCTGCTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATtggt                                                                                                                         >  1:60699/1‑148 (MQ=255)
                                       tCTGCTTAAGTTGAGTGGCGAAGCTCTGCAGGTCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTc                                                                                                                                             <  8:389723/113‑1 (MQ=255)
                                       tCTGCTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTc                                                                                                                                             >  7:389723/1‑113 (MQ=255)
                                       tCTGCTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTc                                                                                                          >  6:131763/1‑148 (MQ=255)
                                       tCTGCTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTc                                                                                                          >  6:137345/1‑148 (MQ=255)
                                       tCTACTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTa                                                                                                                  <  7:230721/140‑1 (MQ=255)
                                       tCTACTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTa                                                                                                                  >  8:230721/1‑140 (MQ=255)
                                                    aGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTc                                                                                             <  5:137345/148‑1 (MQ=255)
                                                    aGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTc                                                                                             <  5:131763/148‑1 (MQ=255)
                                                       ggCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTgg                                                                                          >  2:70648/1‑148 (MQ=255)
                                                                  gCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGATATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGTGAAAGCggg                                                                                >  8:320654/1‑147 (MQ=255)
                                                                  gCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGATATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCggg                                                                                >  4:215242/1‑147 (MQ=255)
                                                                   cAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTa                                                                              <  2:108851/148‑1 (MQ=255)
                                                                      ggCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGa                                                                           <  2:60699/148‑1 (MQ=255)
                                                                       gCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTtggtgtggtg                                                                                                                               <  1:297294/95‑1 (MQ=255)
                                                                       gCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTtggtgtggtg                                                                                                                               >  2:297294/1‑95 (MQ=255)
                                                                        cACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGAAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAAc                                                                         <  8:220654/148‑1 (MQ=255)
                                                                            gAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGgcgc                                                                                                  <  4:336418/119‑1 (MQ=255)
                                                                            gAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGgcgc                                                                                                  >  3:336418/1‑119 (MQ=255)
                                                                            gAAGGCTTCGGGATTGATGCAAGCATACTGGATCCTATGGCTCAGGAAATCAAAGAACTGGTTGAAGTGGGGATTCAGGTTGGTGTGTTGATTGGTGGGGGTAACCAGTTCCGTGGCGCTGGTCGGGCGAAAGCGGGTATGAACcgcg                                                                     >  2:52269/1‑148 (MQ=255)
                                                                               ggCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGATATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGTGAAAGCGGGTATGAACCGCGTtg                                                                  <  7:320654/148‑1 (MQ=255)
                                                                               ggCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGATATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTtg                                                                  <  3:215242/148‑1 (MQ=255)
                                                                                gCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGTACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTtgt                                                                 <  1:70648/148‑1 (MQ=255)
                                                                                  ttCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTgg                                                               >  3:41232/1‑148 (MQ=255)
                                                                                    cGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAg                                                                                    >  4:13446/1‑125 (MQ=255)
                                                                                    cGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAg                                                                                    <  3:13446/125‑1 (MQ=255)
                                                                                    cGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTtgtg                                                                >  8:346166/1‑145 (MQ=255)
                                                                                    cGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTtgtg                                                                <  7:346166/145‑1 (MQ=255)
                                                                                          tGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACca                                                        >  8:153961/1‑147 (MQ=255)
                                                                                            aTGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACAt                                                     <  7:153961/148‑1 (MQ=255)
                                                                                              gCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATgg                                                   >  8:42581/1‑148 (MQ=255)
                                                                                                aaGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATgggg                                                 <  7:210364/148‑1 (MQ=255)
                                                                                                   cATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATg                                              >  7:178075/1‑148 (MQ=255)
                                                                                                   cATACTGGATCGTATGGCGCAGGAAATCAAAGAACTGGTTGTCCTGGGTATTCAGGTTGGTGTCGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGACCCGGGTTGTGGGCGACCTCATGGGGATg                                              <  1:52269/148‑1 (MQ=255)
                                                                                                      aCTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGa                                                           >  3:46988/1‑132 (MQ=255)
                                                                                                      aCTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGa                                                           <  4:46988/132‑1 (MQ=255)
                                                                                                      aCTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATgggg                                                 >  2:101545/1‑142 (MQ=255)
                                                                                                      aCTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTg                                           >  6:50243/1‑148 (MQ=255)
                                                                                                      aCTGGATCGTATGGCGCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATgggg                                                 <  1:101545/142‑1 (MQ=255)
                                                                                                         ggATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTtggtgtggtg                                                                                                                               <  5:52938/61‑1 (MQ=255)
                                                                                                         ggATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTtggtgtggtg                                                                                                                               >  6:52938/1‑61 (MQ=255)
                                                                                                            tCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATg                                                    >  3:514/1‑133 (MQ=255)
                                                                                                            tCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATg                                                    <  4:514/133‑1 (MQ=255)
                                                                                                              gTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGCTCTGGCGAAAGAGGGTATGAAACGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCg                                    >  2:273891/1‑147 (MQ=255)
                                                                                                               tATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTa                                  <  8:178075/148‑1 (MQ=255)
                                                                                                                  ggCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGt                                                                               <  3:278516/100‑1 (MQ=255)
                                                                                                                  ggCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGt                                                                               >  4:278516/1‑100 (MQ=255)
                                                                                                                  ggCTCAGGAAATCAAAGAACTGGTTGAACTGGGTAGTCAGGGTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAAt                                >  4:269855/1‑147 (MQ=255)
                                                                                                                      cAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACg                           >  2:315690/1‑148 (MQ=255)
                                                                                                                       aGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACgg                          <  5:50243/148‑1 (MQ=255)
                                                                                                                         gAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATg                                              <  1:57793/126‑1 (MQ=255)
                                                                                                                         gAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATg                                              >  2:57793/1‑126 (MQ=255)
                                                                                                                            aTCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGAcc                                     >  6:8366/1‑132 (MQ=255)
                                                                                                                            aTCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGAcc                                     <  5:8366/132‑1 (MQ=255)
                                                                                                                              cAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACcaca                                                      <  5:40250/113‑1 (MQ=255)
                                                                                                                              cAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACcaca                                                      >  6:40250/1‑113 (MQ=255)
                                                                                                                              cAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCt                       <  4:29326/144‑1 (MQ=255)
                                                                                                                              cAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCt                       >  3:29326/1‑144 (MQ=255)
                                                                                                                                     cTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTg            <  7:42581/148‑1 (MQ=255)
                                                                                                                                      tGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCa                   >  4:137348/1‑140 (MQ=255)
                                                                                                                                      tGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCa                   <  3:137348/140‑1 (MQ=255)
                                                                                                                                        gTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTGTGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCt                       >  4:322768/1‑134 (MQ=255)
                                                                                                                                        gTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTGTGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCt                       <  3:322768/134‑1 (MQ=255)
                                                                                                                                         ttGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGAtgc        <  4:41232/148‑1 (MQ=255)
                                                                                                                                              cTGGGTATTCAGGTTGGTGTGGTGATTTGTGGGGGTAACCTGTTCCGTGGCGCTGTTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGTGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGAtgcactgc   <  1:273891/148‑1 (MQ=255)
                                                                                                                                                 ggTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTg            >  8:131315/1‑136 (MQ=255)
                                                                                                                                                 ggTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTg            <  7:131315/136‑1 (MQ=255)
                                                                                                                                                 ggTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGAtgcactgca  <  1:315690/146‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AAACATGGCTACCAATGCAAAACCCGTCTATAAACGCATTCTGCTTAAGTTGAGTGGCGAAGCTCTGCAGGGCACTGAAGGCTTCGGTATTGATGCAAGCATACTGGATCGTATGGCTCAGGAAATCAAAGAACTGGTTGAACTGGGTATTCAGGTTGGTGTGGTGATTGGTGGGGGTAACCTGTTCCGTGGCGCTGGTCTGGCGAAAGCGGGTATGAACCGCGTTGTGGGCGACCACATGGGGATGCTGGCGACCGTAATGAACGGCCTGGCAATGCGTGATGCACTGCA  >  NC_000913/191851‑192141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: