Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,834,784 C→T 5.6% S291S (AGC→AGT norW → NADH:flavorubredoxin oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,834,7840CT 5.6% 238.7 / 2.5 71S291S (AGC→AGTnorWNADH:flavorubredoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (36/31);  new base T (2/2);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

TGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATACCGA  >  NC_000913/2834639‑2834930
                                                                                                                                                 |                                                                                                                                                  
tGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAgcgc                                                                                                                                                  >  7:376498/1‑148 (MQ=255)
  gCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTAGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTATcgcgc                                                                                                                                                >  4:264380/1‑147 (MQ=255)
  gCACGACGCGCCGGGCTCACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTAAGCGCGa                                                                                                                                                <  8:326651/148‑1 (MQ=255)
   cACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGAt                                                                                                                                               <  8:376498/148‑1 (MQ=255)
      gacgCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGcgcg                                                                                                                                                 <  2:176201/143‑1 (MQ=255)
      gacgCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGcgcg                                                                                                                                                 >  1:176201/1‑143 (MQ=255)
        cgcgCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGt                                                                                                                                            >  4:286889/1‑146 (MQ=255)
        cgcgCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGt                                                                                                                                            <  3:286889/146‑1 (MQ=255)
          cgcCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGcgcg                                                                                                                                                 <  1:220336/139‑1 (MQ=255)
          cgcCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGcgcg                                                                                                                                                 >  2:220336/1‑139 (MQ=255)
             cGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGAGTCAACTTAGCGCGATGGTGCTGGc                                                                                                                                      >  3:229831/1‑147 (MQ=255)
             cGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGAATTGCCGTTCCTCCAGCCGCTTCAACTTAGCGCGATGGTGCTGGc                                                                                                                                      >  8:188857/1‑147 (MQ=255)
                    aCGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAAt                                                                                                                               <  1:309618/147‑1 (MQ=255)
                      gATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCATCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAAtctt                                                                                                                            <  7:188857/148‑1 (MQ=255)
                        ttAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGcgcg                                                                                                                                                 >  1:106132/1‑125 (MQ=255)
                        ttAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGcgcg                                                                                                                                                 <  2:106132/125‑1 (MQ=255)
                                  cgTGTGAGTCGATAGTTATCTGCAAACCAGTAATACCGATAGTTACGCGCTGGGCGATTGCGCGGAAATTACCGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGGGCTGGCAAAAACTCTTCTCGGCAATAac                                                                                                                <  5:262135/148‑1 (MQ=255)
                                             aTAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGaa                                                                                                     <  4:229831/148‑1 (MQ=255)
                                               aGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAAc                                                                                                   >  6:336238/1‑148 (MQ=255)
                                                  tATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAAtcttc                                                                                                                           >  2:168212/1‑121 (MQ=255)
                                                  tATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAAtcttc                                                                                                                           <  1:168212/121‑1 (MQ=255)
                                                      tGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGaa                                                                                                     <  1:51452/139‑1 (MQ=255)
                                                      tGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGaa                                                                                                     >  2:51452/1‑139 (MQ=255)
                                                                 aaTACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTg                                                                                                       >  5:282756/1‑126 (MQ=255)
                                                                 aaTACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTg                                                                                                       <  6:282756/126‑1 (MQ=255)
                                                                 aaTACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGa                                                                                          >  3:38350/1‑139 (MQ=255)
                                                                    aCCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCaaaaa                                                                                                                                 >  1:118465/1‑97 (MQ=255)
                                                                    aCCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCaaaaa                                                                                                                                 <  2:118465/97‑1 (MQ=255)
                                                                    aCCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAacac                                                                                                              >  7:240734/1‑116 (MQ=255)
                                                                    aCCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAacac                                                                                                              <  8:240734/116‑1 (MQ=255)
                                                                    aCCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGaaaa                                                                              >  7:296653/1‑148 (MQ=255)
                                                                    aCCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGaaaa                                                                              >  1:83303/1‑148 (MQ=255)
                                                                    aCCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGaaaa                                                                              >  5:162843/1‑148 (MQ=255)
                                                                         tatTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCATGTATTTCAGTTCCTCAAGCAGATTCAACTTAGCGCGATGGTGCTTGCAAAAAATCTTCTCGTCAATTACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCaaa                                                                         <  3:264380/148‑1 (MQ=255)
                                                                         tatTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCaaa                                                                         <  6:162843/148‑1 (MQ=255)
                                                                         tatTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCaaa                                                                         <  5:183748/148‑1 (MQ=255)
                                                                           tttACGCGCTGGGCGATTGCGCGGAATTTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCaaaaaa                                                                                                                                <  3:261609/91‑1 (MQ=255)
                                                                            ttACTCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCAGAATCAACTTAGCGCGATGGTGCAGGCAAAAAAt                                                                                                                               >  4:261609/1‑91 (MQ=255)
                                                                            ttACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACg                                                                      >  7:46004/1‑148 (MQ=255)
                                                                             tACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGc                                                                     >  8:78029/1‑148 (MQ=255)
                                                                                 cgcTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCgg                                                                  >  4:237169/1‑147 (MQ=255)
                                                                                 cgcTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGa                                                                 <  7:78029/148‑1 (MQ=255)
                                                                                 cgcTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGa                                                                 <  5:336238/148‑1 (MQ=255)
                                                                                 cgcTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGa                                                                 <  3:237169/148‑1 (MQ=255)
                                                                                      ggCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAAtt                                                              <  2:83303/146‑1 (MQ=255)
                                                                                       gCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCaaa                                                                         <  1:310922/134‑1 (MQ=255)
                                                                                       gCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCaaa                                                                         >  2:310922/1‑134 (MQ=255)
                                                                                            tGCGCGGAAATTAACGGTCAGCTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATACCCCGCCGCTGAAACTCCCGGCCATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTg                                                       >  5:365925/1‑147 (MQ=255)
                                                                                                              cAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAAccc                                    >  4:248672/1‑148 (MQ=255)
                                                                                                                 gTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGc                                 >  2:274942/1‑148 (MQ=255)
                                                                                                                  tATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCg                                >  6:349295/1‑148 (MQ=255)
                                                                                                                  tATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCg                                <  3:248672/148‑1 (MQ=255)
                                                                                                                     tGCCGTTCCTCCAGCCGATTCAACTTAGTGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCaaa                                                                         >  8:292214/1‑104 (MQ=255)
                                                                                                                     tGCCGTTCCTCCAGCCGATTCAACTTAGTGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCaaa                                                                         <  7:292214/104‑1 (MQ=255)
                                                                                                                     tGCCGTTCCTCCAGCCGATTCAACTTAGTGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAg                                  >  5:238178/1‑143 (MQ=255)
                                                                                                                     tGCCGTTCCTCCAGCCGATTCAACTTAGTGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAg                                  <  6:238178/143‑1 (MQ=255)
                                                                                                                     tGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCa                                   <  4:54113/142‑1 (MQ=255)
                                                                                                                     tGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCa                                   >  3:54113/1‑142 (MQ=255)
                                                                                                                     tGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGt                               >  3:307464/1‑146 (MQ=255)
                                                                                                                      gCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAg                            >  7:216078/1‑148 (MQ=255)
                                                                                                                           tcctccAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGAtt                        >  2:176701/1‑147 (MQ=255)
                                                                                                                             ctccAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTAc                     <  8:46004/148‑1 (MQ=255)
                                                                                                                              tccAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACg                    >  1:63497/1‑148 (MQ=255)
                                                                                                                               ccAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGc                   >  7:172516/1‑148 (MQ=255)
                                                                                                                                 aGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTg                 >  2:358920/1‑148 (MQ=255)
                                                                                                                                  gCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTgg                >  1:293556/1‑148 (MQ=255)
                                                                                                                                     gATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCaa             <  2:293556/148‑1 (MQ=255)
                                                                                                                                     gATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCaa             <  8:172516/148‑1 (MQ=255)
                                                                                                                                       ttCAACTTAGCGCGATGGTGCTGGCAAATAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAAACCCGGAATTCCCGCTGCATCTGTCAGGAGAAACCAAGCGGCAGGATTTACGCTGGCAAAt           >  2:265393/1‑148 (MQ=255)
                                                                                                                                       ttCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAAt           >  3:50631/1‑148 (MQ=255)
                                                                                                                                        tCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAAt           >  7:84718/1‑147 (MQ=255)
                                                                                                                                          aaCTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTa         <  5:349295/147‑1 (MQ=255)
                                                                                                                                          aaCTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTa         <  1:274942/147‑1 (MQ=255)
                                                                                                                                          aaCTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTa         <  1:176701/147‑1 (MQ=255)
                                                                                                                                            cTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATa      >  7:366990/1‑148 (MQ=255)
                                                                                                                                            cTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATa      <  1:358920/148‑1 (MQ=255)
                                                                                                                                              tAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCaa             <  3:57691/139‑1 (MQ=255)
                                                                                                                                              tAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCaa             >  4:57691/1‑139 (MQ=255)
                                                                                                                                              tAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATAcc    >  3:77031/1‑148 (MQ=255)
                                                                                                                                                gcgcGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATACCGa  <  4:77031/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TGGCACGACGCGCCGGGCTGACGATTAATCGCGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATACCGATATTTACGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCGATGGTGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCGGAATTACCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATACCGA  >  NC_000913/2834639‑2834930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: